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The map label for this gene is truA [H]

Identifier: 238916297

GI number: 238916297

Start: 353050

End: 353811

Strand: Direct

Name: truA [H]

Synonym: EUBELI_00331

Alternate gene names: 238916297

Gene position: 353050-353811 (Clockwise)

Preceding gene: 238916296

Following gene: 238916298

Centisome position: 16.47

GC content: 42.52

Gene sequence:

>762_bases
ATGCGAAGAATAAAGCTTATCGTAGCTTATGATGGAACAGAATATTCCGGCTGGCAGATTCAGCCGGAAGCACCAACTAT
AGAAATGTGTCTTGACAAGGCAATTCGCGAGCTTACAGGTGAAAATGTGCATGTGACCGGAGCAAGCCGTACAGACGCAG
GTGTGCATGCATATGGAAATGTAGCTGTATTTGATACGGAATCGACAATACCTGGAGACAGATTCACATTTGCACTAAAC
AGATTCCTGCCGGATTCAATTGTTATTCAGGATTCATGGGAAGTGTCTGGAGATTTTCACCCAAGGCACTGTAACACAAG
AAAGACTTACGAATACCGCATACTTAATACTGTTGTTCCGTTGCCTCAGAAGCGCAATTTCACATGGCATGTTACTGGAA
GTATTGATATTGAAAAGATGAAAGAAGCTGCCGCATATATTGTTGGTGAACATGATTTCAAAAGCTTCTGCTGTGTGAGA
ACTCAGGCAGAATCGACTGTCAGGACTATATATTCACTTGAGGTTCTGCAGGAAGGCAGTGAGATAATAATCCGTATTAA
AGGAAATGGATTCTTATACAACATGGTAAGAATTATTACAGGCACGCTTATTCAGGTAGGAAAAGGAAGATTCAAGCCTG
AATATGTGAAGCAGATGTTAGAGGCAAAGGACAGGACTGTTGCAGGGCAGACTGCTCCTCCACAGGGACTTACACTTGTT
GGCATTGAATATGTGGATAATGATGACGCAAGGGTTGTCTGA

Upstream 100 bases:

>100_bases
CATTATTGTGGTTAAGGTTACGCTTAAGTTAATATAATCAGTGTGTATACAGATATGACATTTATAATAGGCTGATTCTG
GCTGAATAAGGAGGAACGCC

Downstream 100 bases:

>100_bases
TGTGGATAACTTTTTTGGATAAAACATTTGCAAAAGTATATTGACACACGTGGTTATGTGTTATATAATTCAGAAATGTG
GCGTGATAAGTGCGCCCATA

Product: tRNA-pseudouridine synthase I

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MRRIKLIVAYDGTEYSGWQIQPEAPTIEMCLDKAIRELTGENVHVTGASRTDAGVHAYGNVAVFDTESTIPGDRFTFALN
RFLPDSIVIQDSWEVSGDFHPRHCNTRKTYEYRILNTVVPLPQKRNFTWHVTGSIDIEKMKEAAAYIVGEHDFKSFCCVR
TQAESTVRTIYSLEVLQEGSEIIIRIKGNGFLYNMVRIITGTLIQVGKGRFKPEYVKQMLEAKDRTVAGQTAPPQGLTLV
GIEYVDNDDARVV

Sequences:

>Translated_253_residues
MRRIKLIVAYDGTEYSGWQIQPEAPTIEMCLDKAIRELTGENVHVTGASRTDAGVHAYGNVAVFDTESTIPGDRFTFALN
RFLPDSIVIQDSWEVSGDFHPRHCNTRKTYEYRILNTVVPLPQKRNFTWHVTGSIDIEKMKEAAAYIVGEHDFKSFCCVR
TQAESTVRTIYSLEVLQEGSEIIIRIKGNGFLYNMVRIITGTLIQVGKGRFKPEYVKQMLEAKDRTVAGQTAPPQGLTLV
GIEYVDNDDARVV
>Mature_253_residues
MRRIKLIVAYDGTEYSGWQIQPEAPTIEMCLDKAIRELTGENVHVTGASRTDAGVHAYGNVAVFDTESTIPGDRFTFALN
RFLPDSIVIQDSWEVSGDFHPRHCNTRKTYEYRILNTVVPLPQKRNFTWHVTGSIDIEKMKEAAAYIVGEHDFKSFCCVR
TQAESTVRTIYSLEVLQEGSEIIIRIKGNGFLYNMVRIITGTLIQVGKGRFKPEYVKQMLEAKDRTVAGQTAPPQGLTLV
GIEYVDNDDARVV

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=266, Percent_Identity=31.5789473684211, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI199559785, Length=259, Percent_Identity=28.5714285714286, Blast_Score=71, Evalue=8e-13,
Organism=Escherichia coli, GI1788657, Length=248, Percent_Identity=36.2903225806452, Blast_Score=162, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI71985046, Length=265, Percent_Identity=26.4150943396226, Blast_Score=81, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6321112, Length=243, Percent_Identity=27.5720164609054, Blast_Score=72, Evalue=7e-14,
Organism=Drosophila melanogaster, GI116007752, Length=305, Percent_Identity=27.5409836065574, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 28523; Mature: 28523

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRIKLIVAYDGTEYSGWQIQPEAPTIEMCLDKAIRELTGENVHVTGASRTDAGVHAYGN
CCEEEEEEEECCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEECC
VAVFDTESTIPGDRFTFALNRFLPDSIVIQDSWEVSGDFHPRHCNTRKTYEYRILNTVVP
EEEEECCCCCCCCCEEEEEHHHCCCEEEEEECCCCCCCCCCCCCCCCCEEEEEEEEEECC
LPQKRNFTWHVTGSIDIEKMKEAAAYIVGEHDFKSFCCVRTQAESTVRTIYSLEVLQEGS
CCCCCCEEEEEECCCCHHHHHHHHHEEECCCCCCCEEEEECCHHHHHHHHHHHHHHHCCC
EIIIRIKGNGFLYNMVRIITGTLIQVGKGRFKPEYVKQMLEAKDRTVAGQTAPPQGLTLV
EEEEEECCCCEEHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEECCCCCCCCCEEEE
GIEYVDNDDARVV
EEEEECCCCCEEC
>Mature Secondary Structure
MRRIKLIVAYDGTEYSGWQIQPEAPTIEMCLDKAIRELTGENVHVTGASRTDAGVHAYGN
CCEEEEEEEECCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEECC
VAVFDTESTIPGDRFTFALNRFLPDSIVIQDSWEVSGDFHPRHCNTRKTYEYRILNTVVP
EEEEECCCCCCCCCEEEEEHHHCCCEEEEEECCCCCCCCCCCCCCCCCEEEEEEEEEECC
LPQKRNFTWHVTGSIDIEKMKEAAAYIVGEHDFKSFCCVRTQAESTVRTIYSLEVLQEGS
CCCCCCEEEEEECCCCHHHHHHHHHEEECCCCCCCEEEEECCHHHHHHHHHHHHHHHCCC
EIIIRIKGNGFLYNMVRIITGTLIQVGKGRFKPEYVKQMLEAKDRTVAGQTAPPQGLTLV
EEEEEECCCCEEHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEECCCCCCCCCEEEE
GIEYVDNDDARVV
EEEEECCCCCEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA