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The map label for this gene is proS

Identifier: 238916041

GI number: 238916041

Start: 79484

End: 80923

Strand: Direct

Name: proS

Synonym: EUBELI_00074

Alternate gene names: 238916041

Gene position: 79484-80923 (Clockwise)

Preceding gene: 238916040

Following gene: 238916043

Centisome position: 3.71

GC content: 42.71

Gene sequence:

>1440_bases
ATGGCAGAAGATAAGAAATTAGTAGAAGCGATTACATCCATGAAAGAGGATTTCGCTCAGTGGTACACAGATGTGTGTAA
GAAGGCAGAGCTTATGTCTTACTCAAGCGTCAAGGGATGCATGATTTTTAAGCCGGCTGGATATGCAATCTGGGAGAATA
TTAAGAATGAGATGGACAGAAGATTCAAGGAAACAGGTGTTGAGAATGTTTATCTTCCAATGTTCATTCCAGAGAGCCTT
CTTGAGGTTGAGAAGGATCATGTAGAAGGATTTGCACCAGAAGTTGCATGGGTAACTTATGGCGGACTTAATCCACTTCA
GGAAAGAATGTGTGTAAGACCTACATCAGAGACACTTTTCTGTGATTTCTATAAAGATGAGATACAGTCATACAGAGATT
TACCAAAGGTATATAACCAGTGGTGTTCAGTTGTAAGATGGGAGAAGGAGACAAGACCATTCCTTCGTTCAAGAGAGTTC
TTATGGCAGGAAGGCCATACAGCACATGCTACAGCAGAAGAGGCTGAGGCACGTACACAGCAGATGCTTAACCTTTACGC
TGACTTCTGTGAGGAAGTTCTTGCAATTCCTGTAATTAAGGGACGTAAGACTGATAAGGAGAAGTTTGCAGGTGCAGAGG
CTACGTATACTATCGAGGCGCTTATGCATGATGGTAAGGCCCTCCAGTCAGGTACAAGCCATAACTTTGGTGATGGATTT
GCCAAAGCATTTGGTATCCAGTACACAGATAAGGATAATAAGTTAAAATATGTTCACCAGACTTCATGGGGAACAACAAC
AAGACTTATCGGTGCTGTTATCATGACTCATGGTGATGATTCAGGACTTGTGCTTCCACCTAAGGTTGCACCTGTTCAGG
TTGATGTTATTCCTATTATGCAGAAGAAGGCAGGCGTTCTTGACAAGGCTTATGAAGTTGGCCAGGCTCTTAAGGCAGCA
GGACTTCGTGTTAAGGTTGATGATTCAGATAAGAATCCTGGCTGGAAGTTCTCTGAGCAGGAGATGCGTGGTATCCCTGT
AAGAGTTGAGATGGGACCAAGAGATATTGAGGCTAATCAGGCTGTTATTGTTCGTCGTGATACAAGAGAGAAGATTACTG
TATCCATCGATGAGCTTGCAGACAGAATTCCAGAAATTCTTGATACTATCCAGAAGGATATGCTTGAGAGAGCAAGAGCT
CACAGAGAAGCTCATACATATACAGCTCTTAATTATGATGAATTCAAGGATACAGCAGCTAATAAGGCTGGATTCATCAA
GGCTATGTGGTGTGGAGACCAGGCTTGTGAGGACAAGATTAAGGAAGATACTACATGTACATCAAGATGTATGCCATTTG
CACAGGAGAAGTTAAGTGATGTGTGCGTATGCTGTGGTAAGCCGGCTAAGGCTATGGTTTACTGGGGTAAGGCATATTAA

Upstream 100 bases:

>100_bases
ACTCTAGTGGTGCCGTCAATGGCAGGCGGACAATGCGAAGCAGCATTCGCATCAGGCTCCGTATGAAACGGGTCTGTAAT
GTCATAGAAAGAGGTTTATT

Downstream 100 bases:

>100_bases
TTCTAAGAAGTGTTAATTATAATAACCCTCATCGGATTTGCCAGAAGGCAGCCGGTGAGGGTTATTTTATGATTATATTA
TTTGATATGTTCAATCATTG

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MAEDKKLVEAITSMKEDFAQWYTDVCKKAELMSYSSVKGCMIFKPAGYAIWENIKNEMDRRFKETGVENVYLPMFIPESL
LEVEKDHVEGFAPEVAWVTYGGLNPLQERMCVRPTSETLFCDFYKDEIQSYRDLPKVYNQWCSVVRWEKETRPFLRSREF
LWQEGHTAHATAEEAEARTQQMLNLYADFCEEVLAIPVIKGRKTDKEKFAGAEATYTIEALMHDGKALQSGTSHNFGDGF
AKAFGIQYTDKDNKLKYVHQTSWGTTTRLIGAVIMTHGDDSGLVLPPKVAPVQVDVIPIMQKKAGVLDKAYEVGQALKAA
GLRVKVDDSDKNPGWKFSEQEMRGIPVRVEMGPRDIEANQAVIVRRDTREKITVSIDELADRIPEILDTIQKDMLERARA
HREAHTYTALNYDEFKDTAANKAGFIKAMWCGDQACEDKIKEDTTCTSRCMPFAQEKLSDVCVCCGKPAKAMVYWGKAY

Sequences:

>Translated_479_residues
MAEDKKLVEAITSMKEDFAQWYTDVCKKAELMSYSSVKGCMIFKPAGYAIWENIKNEMDRRFKETGVENVYLPMFIPESL
LEVEKDHVEGFAPEVAWVTYGGLNPLQERMCVRPTSETLFCDFYKDEIQSYRDLPKVYNQWCSVVRWEKETRPFLRSREF
LWQEGHTAHATAEEAEARTQQMLNLYADFCEEVLAIPVIKGRKTDKEKFAGAEATYTIEALMHDGKALQSGTSHNFGDGF
AKAFGIQYTDKDNKLKYVHQTSWGTTTRLIGAVIMTHGDDSGLVLPPKVAPVQVDVIPIMQKKAGVLDKAYEVGQALKAA
GLRVKVDDSDKNPGWKFSEQEMRGIPVRVEMGPRDIEANQAVIVRRDTREKITVSIDELADRIPEILDTIQKDMLERARA
HREAHTYTALNYDEFKDTAANKAGFIKAMWCGDQACEDKIKEDTTCTSRCMPFAQEKLSDVCVCCGKPAKAMVYWGKAY
>Mature_478_residues
AEDKKLVEAITSMKEDFAQWYTDVCKKAELMSYSSVKGCMIFKPAGYAIWENIKNEMDRRFKETGVENVYLPMFIPESLL
EVEKDHVEGFAPEVAWVTYGGLNPLQERMCVRPTSETLFCDFYKDEIQSYRDLPKVYNQWCSVVRWEKETRPFLRSREFL
WQEGHTAHATAEEAEARTQQMLNLYADFCEEVLAIPVIKGRKTDKEKFAGAEATYTIEALMHDGKALQSGTSHNFGDGFA
KAFGIQYTDKDNKLKYVHQTSWGTTTRLIGAVIMTHGDDSGLVLPPKVAPVQVDVIPIMQKKAGVLDKAYEVGQALKAAG
LRVKVDDSDKNPGWKFSEQEMRGIPVRVEMGPRDIEANQAVIVRRDTREKITVSIDELADRIPEILDTIQKDMLERARAH
REAHTYTALNYDEFKDTAANKAGFIKAMWCGDQACEDKIKEDTTCTSRCMPFAQEKLSDVCVCCGKPAKAMVYWGKAY

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro)

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily

Homologues:

Organism=Homo sapiens, GI62241042, Length=493, Percent_Identity=44.421906693712, Blast_Score=414, Evalue=1e-116,
Organism=Escherichia coli, GI1786392, Length=134, Percent_Identity=27.6119402985075, Blast_Score=66, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI71991342, Length=495, Percent_Identity=41.8181818181818, Blast_Score=394, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI71991346, Length=363, Percent_Identity=46.831955922865, Blast_Score=350, Evalue=1e-96,
Organism=Saccharomyces cerevisiae, GI6321808, Length=510, Percent_Identity=43.921568627451, Blast_Score=437, Evalue=1e-123,
Organism=Drosophila melanogaster, GI24649468, Length=501, Percent_Identity=43.5129740518962, Blast_Score=400, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24649466, Length=501, Percent_Identity=43.5129740518962, Blast_Score=399, Evalue=1e-111,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYP_EUBE2 (C4Z1D7)

Other databases:

- EMBL:   CP001104
- RefSeq:   YP_002929558.1
- GeneID:   7956809
- GenomeReviews:   CP001104_GR
- KEGG:   eel:EUBELI_00074
- OMA:   PGWKFNE
- ProtClustDB:   PRK08661
- GO:   GO:0005737
- HAMAP:   MF_01571
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004499
- InterPro:   IPR017449
- InterPro:   IPR016061
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.30.110.30
- PANTHER:   PTHR11451:SF6
- PRINTS:   PR01046
- SMART:   SM00946
- TIGRFAMs:   TIGR00408

Pfam domain/function: PF03129 HGTP_anticodon; PF09180 ProRS-C_1; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd; SSF64586 Pro-tRNA_synth_II_C

EC number: =6.1.1.15

Molecular weight: Translated: 54405; Mature: 54273

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEDKKLVEAITSMKEDFAQWYTDVCKKAELMSYSSVKGCMIFKPAGYAIWENIKNEMDR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHH
RFKETGVENVYLPMFIPESLLEVEKDHVEGFAPEVAWVTYGGLNPLQERMCVRPTSETLF
HHHHCCCCCEEEEEECCHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHCCCCCCCEE
CDFYKDEIQSYRDLPKVYNQWCSVVRWEKETRPFLRSREFLWQEGHTAHATAEEAEARTQ
EHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
QMLNLYADFCEEVLAIPVIKGRKTDKEKFAGAEATYTIEALMHDGKALQSGTSHNFGDGF
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCHHEEHHHHHHCCHHHHCCCCCCCCCHH
AKAFGIQYTDKDNKLKYVHQTSWGTTTRLIGAVIMTHGDDSGLVLPPKVAPVQVDVIPIM
HHHHCCEEECCCCCEEEEEECCCCHHHHHHHHHHEEECCCCCCEECCCCCCEEEEEEEHH
QKKAGVLDKAYEVGQALKAAGLRVKVDDSDKNPGWKFSEQEMRGIPVRVEMGPRDIEANQ
HHHCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCHHHHCCCCEEEECCCCCCCCCC
AVIVRRDTREKITVSIDELADRIPEILDTIQKDMLERARAHREAHTYTALNYDEFKDTAA
EEEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHC
NKAGFIKAMWCGDQACEDKIKEDTTCTSRCMPFAQEKLSDVCVCCGKPAKAMVYWGKAY
CCCCCEEEEECCCHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHCCCCCCEEEEECCCC
>Mature Secondary Structure 
AEDKKLVEAITSMKEDFAQWYTDVCKKAELMSYSSVKGCMIFKPAGYAIWENIKNEMDR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHH
RFKETGVENVYLPMFIPESLLEVEKDHVEGFAPEVAWVTYGGLNPLQERMCVRPTSETLF
HHHHCCCCCEEEEEECCHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHCCCCCCCEE
CDFYKDEIQSYRDLPKVYNQWCSVVRWEKETRPFLRSREFLWQEGHTAHATAEEAEARTQ
EHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
QMLNLYADFCEEVLAIPVIKGRKTDKEKFAGAEATYTIEALMHDGKALQSGTSHNFGDGF
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCHHEEHHHHHHCCHHHHCCCCCCCCCHH
AKAFGIQYTDKDNKLKYVHQTSWGTTTRLIGAVIMTHGDDSGLVLPPKVAPVQVDVIPIM
HHHHCCEEECCCCCEEEEEECCCCHHHHHHHHHHEEECCCCCCEECCCCCCEEEEEEEHH
QKKAGVLDKAYEVGQALKAAGLRVKVDDSDKNPGWKFSEQEMRGIPVRVEMGPRDIEANQ
HHHCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCHHHHCCCCEEEECCCCCCCCCC
AVIVRRDTREKITVSIDELADRIPEILDTIQKDMLERARAHREAHTYTALNYDEFKDTAA
EEEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHC
NKAGFIKAMWCGDQACEDKIKEDTTCTSRCMPFAQEKLSDVCVCCGKPAKAMVYWGKAY
CCCCCEEEEECCCHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHCCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA