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The map label for this gene is hprA [H]

Identifier: 238916024

GI number: 238916024

Start: 60777

End: 61739

Strand: Direct

Name: hprA [H]

Synonym: EUBELI_00057

Alternate gene names: 238916024

Gene position: 60777-61739 (Clockwise)

Preceding gene: 238916023

Following gene: 238916027

Centisome position: 2.83

GC content: 41.95

Gene sequence:

>963_bases
ATGAAGATACTTATGTTGGAAACTATGACATACGGGGATGATATTTCGCTTGAGCAGTTTAACAGGCTTGGCGAGGTTGT
GACATACAGCTTAAGTTCTTATGAGGAAGCTGTTGCAAGAATGAAAGAGCATAATCCTGATGTCGTTATTATTAATAAAA
CTAACATGAACGCAGAGTGCATGGCAGCTGCGCCTAATCTTAAGATGGTGGCAGAGGCAGCTACAGGCTACAACAATATT
GATATAGAATATGCCAAAGCGCATGGAATAAGGGTTGCCAATGTTGCCGGATATTCAACACAGTCAGTTATACAGCACAC
ATTTGCACTTGCATTTTATCTTATCGAGAAGATGAGATATTATGATGACTTCGTAAAGAGCGGTGAGTATGCCGAGTGGC
CTTGCTTCTCACATTTTGCAAATGTGTTTCACGAACTGGCAGGAAAGACATGGGGAATTGTCGGACTTGGCAATATAGGA
AGAGGCGTTGCTAAGATTGCGGCAGACTTTGGCTGTAATGTAATATACTATTCAGCAAGCGGTCACAGCTATGATGTGCC
ATACGAAAGATATGAGCTTGATGAATTTTTGAAGAAATCGGACATTGTGTCAATTCATGCACCGCTTAACAAATATACAG
AAAATCTGTTTAATTATGAGACACTTAAGAAGATGAAGAGCAGCGCAGTTATTCTGAATCTTGGACGTGGCCCGATTGTT
AATGACGCAGACCTTGTAAAAGCGCTTAACGAAGGCGTGATTGCAGCGGCAGGACTTGATGTAATAACTACTGAGCCGGT
TGTTAAGGACAATCCTCTTCTTACAATTAAGGACAGCAACAAGCTTATCGTTACCCCTCATGTTGCATGGGCGACATACG
AGGCGAGAACCCGCCTGATGGACGAGATATATCTTAATATCAGGGCATATATGGACGGGGAAGAGCGAAATGTTATTGTT
TAA

Upstream 100 bases:

>100_bases
AAATGTAGACAGGGAGCTTGTGAAACTAAATAAACAGTACAGGGAAATTATTAAAAATGTACGATAATTATGCAAAAATG
TGCAGAAACGGAGGATTATT

Downstream 100 bases:

>100_bases
TGTATGGTAATAATTAGATGTTAAAAAGAAGAATGCTTTTTTAAAACGCAATATATCAAATAAGATTGATATTCTTCGCG
CAAAAAGTCAATGAATTTCT

Product: (R)-2-hydroxyacid dehydrogenase

Products: NA

Alternate protein names: GDH; Glyoxylate reductase; Hydroxypyruvate dehydrogenase; NADH-dependent hydroxypyruvate reductase; HPR; HPR-A [H]

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MKILMLETMTYGDDISLEQFNRLGEVVTYSLSSYEEAVARMKEHNPDVVIINKTNMNAECMAAAPNLKMVAEAATGYNNI
DIEYAKAHGIRVANVAGYSTQSVIQHTFALAFYLIEKMRYYDDFVKSGEYAEWPCFSHFANVFHELAGKTWGIVGLGNIG
RGVAKIAADFGCNVIYYSASGHSYDVPYERYELDEFLKKSDIVSIHAPLNKYTENLFNYETLKKMKSSAVILNLGRGPIV
NDADLVKALNEGVIAAAGLDVITTEPVVKDNPLLTIKDSNKLIVTPHVAWATYEARTRLMDEIYLNIRAYMDGEERNVIV

Sequences:

>Translated_320_residues
MKILMLETMTYGDDISLEQFNRLGEVVTYSLSSYEEAVARMKEHNPDVVIINKTNMNAECMAAAPNLKMVAEAATGYNNI
DIEYAKAHGIRVANVAGYSTQSVIQHTFALAFYLIEKMRYYDDFVKSGEYAEWPCFSHFANVFHELAGKTWGIVGLGNIG
RGVAKIAADFGCNVIYYSASGHSYDVPYERYELDEFLKKSDIVSIHAPLNKYTENLFNYETLKKMKSSAVILNLGRGPIV
NDADLVKALNEGVIAAAGLDVITTEPVVKDNPLLTIKDSNKLIVTPHVAWATYEARTRLMDEIYLNIRAYMDGEERNVIV
>Mature_320_residues
MKILMLETMTYGDDISLEQFNRLGEVVTYSLSSYEEAVARMKEHNPDVVIINKTNMNAECMAAAPNLKMVAEAATGYNNI
DIEYAKAHGIRVANVAGYSTQSVIQHTFALAFYLIEKMRYYDDFVKSGEYAEWPCFSHFANVFHELAGKTWGIVGLGNIG
RGVAKIAADFGCNVIYYSASGHSYDVPYERYELDEFLKKSDIVSIHAPLNKYTENLFNYETLKKMKSSAVILNLGRGPIV
NDADLVKALNEGVIAAAGLDVITTEPVVKDNPLLTIKDSNKLIVTPHVAWATYEARTRLMDEIYLNIRAYMDGEERNVIV

Specific function: Plays a central role in assimilation of carbon. It converts hydroxypyruvate to glycerate as a key step in the serine cycle, and may also play an important role in C2 reactions, by interconverting glyoxylate and glycolate [H]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308577, Length=299, Percent_Identity=30.4347826086957, Blast_Score=130, Evalue=1e-30,
Organism=Homo sapiens, GI61743967, Length=254, Percent_Identity=31.8897637795276, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI4557497, Length=254, Percent_Identity=31.8897637795276, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI6912396, Length=257, Percent_Identity=30.3501945525292, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI145580578, Length=254, Percent_Identity=30.7086614173228, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI4557499, Length=254, Percent_Identity=30.7086614173228, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI145580575, Length=284, Percent_Identity=30.6338028169014, Blast_Score=119, Evalue=3e-27,
Organism=Escherichia coli, GI87082289, Length=297, Percent_Identity=32.6599326599327, Blast_Score=138, Evalue=4e-34,
Organism=Escherichia coli, GI1787645, Length=260, Percent_Identity=24.6153846153846, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1789279, Length=267, Percent_Identity=26.2172284644195, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI1788660, Length=322, Percent_Identity=26.0869565217391, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI87081824, Length=182, Percent_Identity=29.1208791208791, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17532191, Length=313, Percent_Identity=32.5878594249201, Blast_Score=137, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI25147481, Length=256, Percent_Identity=28.90625, Blast_Score=100, Evalue=7e-22,
Organism=Saccharomyces cerevisiae, GI6324055, Length=332, Percent_Identity=29.2168674698795, Blast_Score=119, Evalue=8e-28,
Organism=Saccharomyces cerevisiae, GI6320925, Length=327, Percent_Identity=27.82874617737, Blast_Score=112, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6322116, Length=327, Percent_Identity=26.9113149847095, Blast_Score=106, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6324964, Length=258, Percent_Identity=28.6821705426357, Blast_Score=96, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6321253, Length=262, Percent_Identity=27.0992366412214, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324980, Length=109, Percent_Identity=33.0275229357798, Blast_Score=67, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24646446, Length=260, Percent_Identity=34.2307692307692, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24646448, Length=260, Percent_Identity=34.2307692307692, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24646452, Length=260, Percent_Identity=34.2307692307692, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24646450, Length=260, Percent_Identity=34.2307692307692, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI62472511, Length=260, Percent_Identity=34.2307692307692, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI28574286, Length=265, Percent_Identity=32.8301886792453, Blast_Score=128, Evalue=4e-30,
Organism=Drosophila melanogaster, GI19921140, Length=306, Percent_Identity=28.7581699346405, Blast_Score=115, Evalue=3e-26,
Organism=Drosophila melanogaster, GI28571528, Length=258, Percent_Identity=29.8449612403101, Blast_Score=114, Evalue=7e-26,
Organism=Drosophila melanogaster, GI45552429, Length=265, Percent_Identity=29.0566037735849, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24585514, Length=265, Percent_Identity=29.0566037735849, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI28574282, Length=265, Percent_Identity=29.0566037735849, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI28574284, Length=265, Percent_Identity=29.0566037735849, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI45551003, Length=265, Percent_Identity=29.0566037735849, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24585516, Length=239, Percent_Identity=28.0334728033473, Blast_Score=99, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.29 [H]

Molecular weight: Translated: 35801; Mature: 35801

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS00670 D_2_HYDROXYACID_DH_2 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILMLETMTYGDDISLEQFNRLGEVVTYSLSSYEEAVARMKEHNPDVVIINKTNMNAEC
CEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEE
MAAAPNLKMVAEAATGYNNIDIEYAKAHGIRVANVAGYSTQSVIQHTFALAFYLIEKMRY
EEECCCHHEEEHHHCCCCCCEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHH
YDDFVKSGEYAEWPCFSHFANVFHELAGKTWGIVGLGNIGRGVAKIAADFGCNVIYYSAS
HHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHCCCCEEEEECC
GHSYDVPYERYELDEFLKKSDIVSIHAPLNKYTENLFNYETLKKMKSSAVILNLGRGPIV
CCCCCCCHHHHHHHHHHHHCCEEEEECCHHHHHHHHHCHHHHHHHCCCEEEEECCCCCCC
NDADLVKALNEGVIAAAGLDVITTEPVVKDNPLLTIKDSNKLIVTPHVAWATYEARTRLM
CCHHHHHHHHCCEEEECCCCEEECCCCCCCCCEEEEECCCEEEEECCHHEEHHHHHHHHH
DEIYLNIRAYMDGEERNVIV
HHHHEEEEEEECCCCCCCCC
>Mature Secondary Structure
MKILMLETMTYGDDISLEQFNRLGEVVTYSLSSYEEAVARMKEHNPDVVIINKTNMNAEC
CEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEE
MAAAPNLKMVAEAATGYNNIDIEYAKAHGIRVANVAGYSTQSVIQHTFALAFYLIEKMRY
EEECCCHHEEEHHHCCCCCCEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHH
YDDFVKSGEYAEWPCFSHFANVFHELAGKTWGIVGLGNIGRGVAKIAADFGCNVIYYSAS
HHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHCCCCEEEEECC
GHSYDVPYERYELDEFLKKSDIVSIHAPLNKYTENLFNYETLKKMKSSAVILNLGRGPIV
CCCCCCCHHHHHHHHHHHHCCEEEEECCHHHHHHHHHCHHHHHHHCCCEEEEECCCCCCC
NDADLVKALNEGVIAAAGLDVITTEPVVKDNPLLTIKDSNKLIVTPHVAWATYEARTRLM
CCHHHHHHHHCCEEEECCCCEEECCCCCCCCCEEEEECCCEEEEECCHHEEHHHHHHHHH
DEIYLNIRAYMDGEERNVIV
HHHHEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8144463; 1729225; 1657886 [H]