Click here to switch to the map view.

The map label for this gene is pfkA [H]

Identifier: 238916027

GI number: 238916027

Start: 62956

End: 64191

Strand: Direct

Name: pfkA [H]

Synonym: EUBELI_00060

Alternate gene names: 238916027

Gene position: 62956-64191 (Clockwise)

Preceding gene: 238916024

Following gene: 238916028

Centisome position: 2.94

GC content: 38.35

Gene sequence:

>1236_bases
ATGAGTATGTTAAAGAATATTCTGGTAGGTCAGTCAGGCGGACCGACATCGGTTATTAATTCATCATTATATGGAGTGAT
TGAGGAAGGACTTAGAAATAAGGAAAAGATTGGCACTGTGTATGGCATGGTGCATGGAATTGAGGGATTTCTGGCAGGCA
ATTTCATTAATCTTTCTGAAGTGGCAGATAATGAGCCAATTGATAGATTAAAGATAACACCGGCTTCTTTTCTTGGAAGT
TGCAGGTATATGCTTCCAGAAGATTTAGGGGATAATGTGTATGATAAATTGTTTGATACATTTGCACAGATGAACATTGG
ATATGTGTTCTATATTGGTGGCAATGATTCAATGGATACAGTGTCAAAATTATCAAGAGTGGCATTATTGAAGAAATCGG
ACATAAGATTTATAGGTGTACCTAAGACAATTGATAATGATCTTGTAATGACAGATCATACTCCAGGGTATGGTTCAACT
GCAAAATATGTTGCTTCAACATTAAAAGAAATTATACTTGACGCAACATGTTATGCACATCCGTCTGTGACAATTGTTGA
ACTTATGGGAAGGCATGCAGGCTGGGTTACAGCGGCATCAGTACTTGCTAGAACAGAGTATAGCAGAAATCCTTATCTTA
TATATATGCCAGAGCATGCATTTGATATGGAAGAATTCAAGAAGTCACTTAAGGCGGCACTTGAACAGGAAACTGCAGTT
GTTGTGTGCGTATCAGAAGGAATTGCGGACAAAGACGGCAGATTCATATGTGAATATGCAGATGCAGCATCGGTTGATGG
TTTTGGACATAAGATGCTTACAGGCTGTGGAAAGTATCTTGAAAATTATGTTAAAGCAGAATTTGGAATCAAATGCAGAA
GTATTGAGCTTAATCTTCCACAGAGATGTTCATCACTTCTTGCTTCAGCAACAGATATTGATGAAGCCGAAAAAGCAGGA
AAGGCAGCGGTTGTAGCAGCTCTTGACGGTGAAACTGGAAAAATGATTACGTTTGTAAGAGATGATACTAACGGATATGA
GATTAATTACGGACTTGCAGATGTGAACGATATATGCAATAAGGAAAAGAAGTTCCCAGCAGAATGGATAACCGCAGATG
GAACAGATATTTCTGATGAGTATATTAAGTATGTAAGACCACTTATTCAGGGAACTAATATTGCGGAGTATTCAGATGGG
GTGCCGGTTCATCTGAAGCCTGCTTATTACAGATAA

Upstream 100 bases:

>100_bases
TCCTATAAGAAAATAAAAAATGCGACTACCGATAGGTATCATGTTGCAATCAATAATCGACAGGTGATACAATCTAAGCA
ACAGCAATAAGGTAGGAGGC

Downstream 100 bases:

>100_bases
CATTAAATATTAAATAAAAATAAAAATGACAGCACGCTATTGACAGCGTGCTGTCATTTTTGTAATATATCAAAGCTACT
TAAGAATTTTATTAAAAGAA

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase [H]

Number of amino acids: Translated: 411; Mature: 410

Protein sequence:

>411_residues
MSMLKNILVGQSGGPTSVINSSLYGVIEEGLRNKEKIGTVYGMVHGIEGFLAGNFINLSEVADNEPIDRLKITPASFLGS
CRYMLPEDLGDNVYDKLFDTFAQMNIGYVFYIGGNDSMDTVSKLSRVALLKKSDIRFIGVPKTIDNDLVMTDHTPGYGST
AKYVASTLKEIILDATCYAHPSVTIVELMGRHAGWVTAASVLARTEYSRNPYLIYMPEHAFDMEEFKKSLKAALEQETAV
VVCVSEGIADKDGRFICEYADAASVDGFGHKMLTGCGKYLENYVKAEFGIKCRSIELNLPQRCSSLLASATDIDEAEKAG
KAAVVAALDGETGKMITFVRDDTNGYEINYGLADVNDICNKEKKFPAEWITADGTDISDEYIKYVRPLIQGTNIAEYSDG
VPVHLKPAYYR

Sequences:

>Translated_411_residues
MSMLKNILVGQSGGPTSVINSSLYGVIEEGLRNKEKIGTVYGMVHGIEGFLAGNFINLSEVADNEPIDRLKITPASFLGS
CRYMLPEDLGDNVYDKLFDTFAQMNIGYVFYIGGNDSMDTVSKLSRVALLKKSDIRFIGVPKTIDNDLVMTDHTPGYGST
AKYVASTLKEIILDATCYAHPSVTIVELMGRHAGWVTAASVLARTEYSRNPYLIYMPEHAFDMEEFKKSLKAALEQETAV
VVCVSEGIADKDGRFICEYADAASVDGFGHKMLTGCGKYLENYVKAEFGIKCRSIELNLPQRCSSLLASATDIDEAEKAG
KAAVVAALDGETGKMITFVRDDTNGYEINYGLADVNDICNKEKKFPAEWITADGTDISDEYIKYVRPLIQGTNIAEYSDG
VPVHLKPAYYR
>Mature_410_residues
SMLKNILVGQSGGPTSVINSSLYGVIEEGLRNKEKIGTVYGMVHGIEGFLAGNFINLSEVADNEPIDRLKITPASFLGSC
RYMLPEDLGDNVYDKLFDTFAQMNIGYVFYIGGNDSMDTVSKLSRVALLKKSDIRFIGVPKTIDNDLVMTDHTPGYGSTA
KYVASTLKEIILDATCYAHPSVTIVELMGRHAGWVTAASVLARTEYSRNPYLIYMPEHAFDMEEFKKSLKAALEQETAVV
VCVSEGIADKDGRFICEYADAASVDGFGHKMLTGCGKYLENYVKAEFGIKCRSIELNLPQRCSSLLASATDIDEAEKAGK
AAVVAALDGETGKMITFVRDDTNGYEINYGLADVNDICNKEKKFPAEWITADGTDISDEYIKYVRPLIQGTNIAEYSDGV
PVHLKPAYYR

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Escherichia coli, GI1790350, Length=369, Percent_Identity=23.5772357723577, Blast_Score=84, Evalue=2e-17,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012829
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 45119; Mature: 44988

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMLKNILVGQSGGPTSVINSSLYGVIEEGLRNKEKIGTVYGMVHGIEGFLAGNFINLSE
CCHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEHHH
VADNEPIDRLKITPASFLGSCRYMLPEDLGDNVYDKLFDTFAQMNIGYVFYIGGNDSMDT
HCCCCCCCEEEECHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHH
VSKLSRVALLKKSDIRFIGVPKTIDNDLVMTDHTPGYGSTAKYVASTLKEIILDATCYAH
HHHHHHHHHHCCCCEEEEECCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCC
PSVTIVELMGRHAGWVTAASVLARTEYSRNPYLIYMPEHAFDMEEFKKSLKAALEQETAV
CCCHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCEE
VVCVSEGIADKDGRFICEYADAASVDGFGHKMLTGCGKYLENYVKAEFGIKCRSIELNLP
EEEEECCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCH
QRCSSLLASATDIDEAEKAGKAAVVAALDGETGKMITFVRDDTNGYEINYGLADVNDICN
HHHHHHHHHHCCCHHHHHCCCEEEEEEECCCCCCEEEEEEECCCCEEEEECCCCHHHHHC
KEKKFPAEWITADGTDISDEYIKYVRPLIQGTNIAEYSDGVPVHLKPAYYR
HHHCCCHHHEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCC
>Mature Secondary Structure 
SMLKNILVGQSGGPTSVINSSLYGVIEEGLRNKEKIGTVYGMVHGIEGFLAGNFINLSE
CHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEHHH
VADNEPIDRLKITPASFLGSCRYMLPEDLGDNVYDKLFDTFAQMNIGYVFYIGGNDSMDT
HCCCCCCCEEEECHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHH
VSKLSRVALLKKSDIRFIGVPKTIDNDLVMTDHTPGYGSTAKYVASTLKEIILDATCYAH
HHHHHHHHHHCCCCEEEEECCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCC
PSVTIVELMGRHAGWVTAASVLARTEYSRNPYLIYMPEHAFDMEEFKKSLKAALEQETAV
CCCHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCEE
VVCVSEGIADKDGRFICEYADAASVDGFGHKMLTGCGKYLENYVKAEFGIKCRSIELNLP
EEEEECCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCH
QRCSSLLASATDIDEAEKAGKAAVVAALDGETGKMITFVRDDTNGYEINYGLADVNDICN
HHHHHHHHHHCCCHHHHHCCCEEEEEEECCCCCCEEEEEEECCCCEEEEECCCCHHHHHC
KEKKFPAEWITADGTDISDEYIKYVRPLIQGTNIAEYSDGVPVHLKPAYYR
HHHCCCHHHEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12840036 [H]