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The map label for this gene is actII-3 [H]

Identifier: 238915998

GI number: 238915998

Start: 29452

End: 31542

Strand: Direct

Name: actII-3 [H]

Synonym: EUBELI_00031

Alternate gene names: 238915998

Gene position: 29452-31542 (Clockwise)

Preceding gene: 238915996

Following gene: 238915999

Centisome position: 1.37

GC content: 37.16

Gene sequence:

>2091_bases
ATGAAAAAGTTTGGAAAAGTTGTTGTTAAGTTAAGAATTCCAATTCTGGTTCTTAGCTTCCTGCTGTTGATTCCATCAGT
TCTTGGTTACTTTAATACCAGGGTTAATTACGATATCCTGTATTATCTGCCAAGTGATATCGACACTATGCAGGGACAGG
ACATTTTGCTTGATGATTTCGGAAAGGGCGCTTATGCAATGGTCGTCGTTGACGGAATGAATAAGTCAAATGTTTCTAAG
TTGGTAAAGAAGGTTGAGGGGGTTGACCATGTAGCGTCAGTTATCTCTTATAGTGGAATAGTAGGAGATGATGTTCCTTC
AGAAATTCTTCCTGATAAGTTCCGTTCTTATTTTGAAAATGAGGATTCAGGAGCTACATTGTTTGCAATATTCTTTGATG
ATACAACATCAAGTGACGATACAATGAAGGCTATTCAGGAAGTAAGAGATGTTACGGATAATCAGTGTTATATTGCCGGT
ATGTCTGCTGTAGTTACAGATACTAAGACAATGGCAGAAAAGGAGACTCCTTTTTATGTACTTGTAGCGGTTGTTCTTGT
ATGTATCGTACTTGCTATATTTATGGATTCATTCCTTGTACCAGTATTCTTTATGTTAAGTATAGGTATGGCAATTGTGT
ATAACCTTGGTTCTAATTATTTCTTAGGTGAGGTTTCATACATAACGAAAGCACTGGCAGCGGTACTTCAGCTTGGTGTA
ACTCTGGATTATTCTATATTCTTATGGCACAGTTACAAGGAAGCTAAGGAAGAAACTCCAGATGATCATCAAGAAGCTAT
GGCGGTTGCTATTGGGAATACACTTACATCAGTTGTTGGTTCTTCCATAACAACTGTTGCAGGATTCATCGCACTCTGCT
TTATGAGCTTTACACTTGGTCTTGACCTTGGTGTTGTTATGGCAAAGGGTGTTGTATTGGGTGTTATTGGTTGTGTTACA
ATACTTCCTTCCCTTATTCTTACATTTGACAAGGCTCTTGAGAAGACAATGCATAGAGAGATTATGCCGAATTTTGATAA
GCCGGCAAGATGGATAGTTAATCATTCATGGATATTCCTTATAATATTCGTTCTGCTTCTTGGCCCGGCAATCTACGGAT
ATAACAATACTAAGGTTTACTATGATTTATCTGATACACTTCCAGAGAAGCTTAACTGTTCGCAGGCTAACAAGCTGCTT
GCCGACAACTTTGACGGAACTAATTCTATATATATGATTCTTGCAGACAGTAATCTTTCAGCAGAGGATTCTAATGCAAT
GATGAATGAGGTAAATGATCTGGACGGAATTTCATTTGCATTAAGTATTGACTCAGCACTCGGTGGAGAAATCCCTACGG
AAATGCTTCCTGATTCGCTTGTTTCAGAGCTTAAGGGTGATGAATACCAGATTATGATGGTATCAACTAATTACACAATT
GCTTCTGATGAAATCAATGACCAGATTAATAAGGTTGATGCAATTGCAAAGAAGTATGATGCTAAATCTATGGTAATTGG
AGAAGCTCCTTGTACTAAGGATCTTATCACTATTACTGATAAGGATTTTAAGACAGTTAGTGCAGTTTCAATTGTTGCGA
TATTCTTTATTATTTTCTTCGTATTAAAGTCAATATCTCTTCCGGTTATTCTGGTTGCTGCTATTGAATTTGCTATATTT
GTTAATATGGGTATTCCATATTATACAGGAACAACGATACCATTTATATCTTCGGTAGTAATCGGAACTATTCAGCTTGG
TGCGACAGTCGATTATGCAATTCTTATGACTACAAGATATAAGAGAGAAAGAGCCGCTGGTAACTCTAAGAAAGAGGCTA
TATCAATAGCACTTGGAACATCTATTCCATCTATTATTGTCAGCGCACTTGGTTTCTTCGCAGCAACATTTGGTGTAGGT
ATGATAGCCAGTGTTGATATGATTGCTTCACTTTGTACACTTATGGCACGAGGCGCAATCATCAGTATGTTTGTCGTTAT
ATTTGTTCTTCCATCACTTTTCGTATTGCTTGATAAGGTGATTATTCATACAAGTTTAGGTTTTAAGCCGAAGAAGAACA
GTCAAAATTAA

Upstream 100 bases:

>100_bases
AATTGAAAATGACTATCGGTCATATTTGTTAAAGACATGAATACTTATATGACTAAGGGTTGTTGGCATTTGCATCTTAC
AGAAAGGCGTGGTTATTTAT

Downstream 100 bases:

>100_bases
ATTAGGAGGATTTATTATGGTAAAGAAGAATTTATTCAGATATAGTTCAGTTTTAATGTGTGTTGTTCTTACTGCGGCAA
GTCTTGTTGGCTGCGGTGCT

Product: hypothetical protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 696; Mature: 696

Protein sequence:

>696_residues
MKKFGKVVVKLRIPILVLSFLLLIPSVLGYFNTRVNYDILYYLPSDIDTMQGQDILLDDFGKGAYAMVVVDGMNKSNVSK
LVKKVEGVDHVASVISYSGIVGDDVPSEILPDKFRSYFENEDSGATLFAIFFDDTTSSDDTMKAIQEVRDVTDNQCYIAG
MSAVVTDTKTMAEKETPFYVLVAVVLVCIVLAIFMDSFLVPVFFMLSIGMAIVYNLGSNYFLGEVSYITKALAAVLQLGV
TLDYSIFLWHSYKEAKEETPDDHQEAMAVAIGNTLTSVVGSSITTVAGFIALCFMSFTLGLDLGVVMAKGVVLGVIGCVT
ILPSLILTFDKALEKTMHREIMPNFDKPARWIVNHSWIFLIIFVLLLGPAIYGYNNTKVYYDLSDTLPEKLNCSQANKLL
ADNFDGTNSIYMILADSNLSAEDSNAMMNEVNDLDGISFALSIDSALGGEIPTEMLPDSLVSELKGDEYQIMMVSTNYTI
ASDEINDQINKVDAIAKKYDAKSMVIGEAPCTKDLITITDKDFKTVSAVSIVAIFFIIFFVLKSISLPVILVAAIEFAIF
VNMGIPYYTGTTIPFISSVVIGTIQLGATVDYAILMTTRYKRERAAGNSKKEAISIALGTSIPSIIVSALGFFAATFGVG
MIASVDMIASLCTLMARGAIISMFVVIFVLPSLFVLLDKVIIHTSLGFKPKKNSQN

Sequences:

>Translated_696_residues
MKKFGKVVVKLRIPILVLSFLLLIPSVLGYFNTRVNYDILYYLPSDIDTMQGQDILLDDFGKGAYAMVVVDGMNKSNVSK
LVKKVEGVDHVASVISYSGIVGDDVPSEILPDKFRSYFENEDSGATLFAIFFDDTTSSDDTMKAIQEVRDVTDNQCYIAG
MSAVVTDTKTMAEKETPFYVLVAVVLVCIVLAIFMDSFLVPVFFMLSIGMAIVYNLGSNYFLGEVSYITKALAAVLQLGV
TLDYSIFLWHSYKEAKEETPDDHQEAMAVAIGNTLTSVVGSSITTVAGFIALCFMSFTLGLDLGVVMAKGVVLGVIGCVT
ILPSLILTFDKALEKTMHREIMPNFDKPARWIVNHSWIFLIIFVLLLGPAIYGYNNTKVYYDLSDTLPEKLNCSQANKLL
ADNFDGTNSIYMILADSNLSAEDSNAMMNEVNDLDGISFALSIDSALGGEIPTEMLPDSLVSELKGDEYQIMMVSTNYTI
ASDEINDQINKVDAIAKKYDAKSMVIGEAPCTKDLITITDKDFKTVSAVSIVAIFFIIFFVLKSISLPVILVAAIEFAIF
VNMGIPYYTGTTIPFISSVVIGTIQLGATVDYAILMTTRYKRERAAGNSKKEAISIALGTSIPSIIVSALGFFAATFGVG
MIASVDMIASLCTLMARGAIISMFVVIFVLPSLFVLLDKVIIHTSLGFKPKKNSQN
>Mature_696_residues
MKKFGKVVVKLRIPILVLSFLLLIPSVLGYFNTRVNYDILYYLPSDIDTMQGQDILLDDFGKGAYAMVVVDGMNKSNVSK
LVKKVEGVDHVASVISYSGIVGDDVPSEILPDKFRSYFENEDSGATLFAIFFDDTTSSDDTMKAIQEVRDVTDNQCYIAG
MSAVVTDTKTMAEKETPFYVLVAVVLVCIVLAIFMDSFLVPVFFMLSIGMAIVYNLGSNYFLGEVSYITKALAAVLQLGV
TLDYSIFLWHSYKEAKEETPDDHQEAMAVAIGNTLTSVVGSSITTVAGFIALCFMSFTLGLDLGVVMAKGVVLGVIGCVT
ILPSLILTFDKALEKTMHREIMPNFDKPARWIVNHSWIFLIIFVLLLGPAIYGYNNTKVYYDLSDTLPEKLNCSQANKLL
ADNFDGTNSIYMILADSNLSAEDSNAMMNEVNDLDGISFALSIDSALGGEIPTEMLPDSLVSELKGDEYQIMMVSTNYTI
ASDEINDQINKVDAIAKKYDAKSMVIGEAPCTKDLITITDKDFKTVSAVSIVAIFFIIFFVLKSISLPVILVAAIEFAIF
VNMGIPYYTGTTIPFISSVVIGTIQLGATVDYAILMTTRYKRERAAGNSKKEAISIALGTSIPSIIVSALGFFAATFGVG
MIASVDMIASLCTLMARGAIISMFVVIFVLPSLFVLLDKVIIHTSLGFKPKKNSQN

Specific function: Participates In The Efflux Of Aminoglycosides. Confers Resistance To A Variety Of These Substances. [C]

COG id: COG1033

COG function: function code R; Predicted exporters of the RND superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mmpL family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004707
- InterPro:   IPR004869
- InterPro:   IPR000731 [H]

Pfam domain/function: PF03176 MMPL [H]

EC number: NA

Molecular weight: Translated: 75953; Mature: 75953

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: PS50156 SSD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKFGKVVVKLRIPILVLSFLLLIPSVLGYFNTRVNYDILYYLPSDIDTMQGQDILLDDF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCEEEEECC
GKGAYAMVVVDGMNKSNVSKLVKKVEGVDHVASVISYSGIVGDDVPSEILPDKFRSYFEN
CCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCHHHHHHHHCC
EDSGATLFAIFFDDTTSSDDTMKAIQEVRDVTDNQCYIAGMSAVVTDTKTMAEKETPFYV
CCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCEEEEECHHHHHCHHHHHHCCCCHHH
LVAVVLVCIVLAIFMDSFLVPVFFMLSIGMAIVYNLGSNYFLGEVSYITKALAAVLQLGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHCC
TLDYSIFLWHSYKEAKEETPDDHQEAMAVAIGNTLTSVVGSSITTVAGFIALCFMSFTLG
CCCEEEEEEECHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
LDLGVVMAKGVVLGVIGCVTILPSLILTFDKALEKTMHREIMPNFDKPARWIVNHSWIFL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCHHHHH
IIFVLLLGPAIYGYNNTKVYYDLSDTLPEKLNCSQANKLLADNFDGTNSIYMILADSNLS
HHHHHHHCCHHCCCCCCEEEEECHHCCCCCCCHHHHHHHHHCCCCCCCEEEEEEECCCCC
AEDSNAMMNEVNDLDGISFALSIDSALGGEIPTEMLPDSLVSELKGDEYQIMMVSTNYTI
CCCHHHHHHHHHCCCCCEEEEEECHHCCCCCCHHHCCHHHHHHHCCCCEEEEEEECCCEE
ASDEINDQINKVDAIAKKYDAKSMVIGEAPCTKDLITITDKDFKTVSAVSIVAIFFIIFF
ECHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHH
VLKSISLPVILVAAIEFAIFVNMGIPYYTGTTIPFISSVVIGTIQLGATVDYAILMTTRY
HHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
KRERAAGNSKKEAISIALGTSIPSIIVSALGFFAATFGVGMIASVDMIASLCTLMARGAI
HHHHHCCCCCHHEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ISMFVVIFVLPSLFVLLDKVIIHTSLGFKPKKNSQN
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MKKFGKVVVKLRIPILVLSFLLLIPSVLGYFNTRVNYDILYYLPSDIDTMQGQDILLDDF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCEEEEECC
GKGAYAMVVVDGMNKSNVSKLVKKVEGVDHVASVISYSGIVGDDVPSEILPDKFRSYFEN
CCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCHHHHHHHHCC
EDSGATLFAIFFDDTTSSDDTMKAIQEVRDVTDNQCYIAGMSAVVTDTKTMAEKETPFYV
CCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCEEEEECHHHHHCHHHHHHCCCCHHH
LVAVVLVCIVLAIFMDSFLVPVFFMLSIGMAIVYNLGSNYFLGEVSYITKALAAVLQLGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHCC
TLDYSIFLWHSYKEAKEETPDDHQEAMAVAIGNTLTSVVGSSITTVAGFIALCFMSFTLG
CCCEEEEEEECHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
LDLGVVMAKGVVLGVIGCVTILPSLILTFDKALEKTMHREIMPNFDKPARWIVNHSWIFL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCHHHHH
IIFVLLLGPAIYGYNNTKVYYDLSDTLPEKLNCSQANKLLADNFDGTNSIYMILADSNLS
HHHHHHHCCHHCCCCCCEEEEECHHCCCCCCCHHHHHHHHHCCCCCCCEEEEEEECCCCC
AEDSNAMMNEVNDLDGISFALSIDSALGGEIPTEMLPDSLVSELKGDEYQIMMVSTNYTI
CCCHHHHHHHHHCCCCCEEEEEECHHCCCCCCHHHCCHHHHHHHCCCCEEEEEEECCCEE
ASDEINDQINKVDAIAKKYDAKSMVIGEAPCTKDLITITDKDFKTVSAVSIVAIFFIIFF
ECHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHH
VLKSISLPVILVAAIEFAIFVNMGIPYYTGTTIPFISSVVIGTIQLGATVDYAILMTTRY
HHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
KRERAAGNSKKEAISIALGTSIPSIIVSALGFFAATFGVGMIASVDMIASLCTLMARGAI
HHHHHCCCCCHHEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ISMFVVIFVLPSLFVLLDKVIIHTSLGFKPKKNSQN
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1878971; 12000953 [H]