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The map label for this gene is gidA [H]

Identifier: 238897248

GI number: 238897248

Start: 5246529

End: 5248418

Strand: Reverse

Name: gidA [H]

Synonym: KP1_5524

Alternate gene names: 238897248

Gene position: 5248418-5246529 (Counterclockwise)

Preceding gene: NA

Following gene: 238897247

Centisome position: 100.0

GC content: 57.41

Gene sequence:

>1890_bases
ATGTTTTATCAGGATCCTTTTGACGTCATTATCATTGGCGGGGGTCATGCAGGCACTGAGGCCGCAATGGCCGCAGCGCG
TATGGGTCAACAAACCCTGCTTTTGACACACAATATCGACACGCTGGGGCAAATGAGTTGCAACCCGGCGATTGGTGGCA
TTGGGAAAGGACACCTGGTAAAAGAAGTGGATGCGCTCGGCGGTTTGATGGCCAAAGCGATCGATCAGGCTGGCATTCAG
TTTAGGATACTAAACGCCAGCAAAGGACCGGCAGTGCGCGCCACCCGCGCCCAGGCTGATCGCGTGCTTTATCGTCAGGC
AGTACGTACCGCGCTGGAGAACCAGCCGAACCTGATGATCTTCCAGCAGGCGGTTGAAGATCTGATTGTCGAAAACGATC
GCGTCGTGGGCGCCGTCACCCAGATGGGCCTCAAATTCCGCGCTAAAGCGGTGGTGCTGACCGTCGGGACCTTCCTTGAC
GGCAAAATCCATATTGGTCTGGACAACTACAGCGGCGGCCGCGCCGGCGATCCGCCGTCTATCCCGCTGTCTCGTCGTCT
GCGTGAATTGCCTCTGCGCGTAAGTCGTCTGAAAACCGGTACTCCGCCGCGTATTGACTCGCGCACCATCGATTTCAGCG
TGCTGGCGCAGCAGCATGGCGATAACCCGATGCCGGTCTTCTCGTTCATGGGATCCGCGGATCAGCATCCGCGCCAGGTA
CCGTGCTATATCACCCACACTAACGAGAAAACCCACGATGTGATCCGCAATAACCTCGATCGCAGTCCTATGTACGCCGG
AGTGATCGAAGGGATCGGACCTCGCTATTGCCCGTCGATCGAAGACAAAGTCATGCGCTTTGCCGATCGCAACCAGCATC
AGATCTTCCTCGAGCCGGAAGGACTGACCTCAAACGAAATTTATCCGAACGGTATATCCACCAGCCTACCGTTTGATGTG
CAAATGCAAATCGTCCGCTCGATGCAGGGGATGGAGAATGCGCGGATCGTCCGTCCAGGCTACGCTATTGAGTACGATTT
CTTCGACCCGCGCGATCTGAAGCCAACGCTTGAGAGCAAATTTATTCAGGGTCTGTTCTTCGCCGGCCAAATCAACGGTA
CCACCGGTTATGAAGAGGCGGCGGCTCAGGGGCTGCTGGCCGGACTTAACGCCGCCCGCTTCTCTGCGGAAAAAGAGGGC
TGGGCGCCGCGTCGCGATCAGGCTTACCTCGGCGTGCTGGTAGATGACCTCTGCACCCTGGGTACCAAAGAACCGTACCG
CATGTTTACTTCCCGCGCTGAATATCGTCTGATGCTGCGCGAAGACAACGCCGATTTGCGTCTGACCGAACAGGGGCGCG
AGCTGGGTCTGGTGGATGACGAACGCTGGGCGCGCTACAACGAGAAGCTGGAAAGCATCGAACGTGAACGCCAGCGTCTG
AAATCCACCTGGGTGAATCCGCAGGCAGAGAGCGCCAACGAAGTGAACGCTCACCTGACCGCGCCGTTGTCGCGTGAAGC
CAGCGGCGAAGATCTGCTGCGTCGTCCGGAAATGACCTACGAACAGCTGGTTCAACTCTCGCCATTCACCCCGGGACTGG
AAGATCGTCAGGCGGCTGAACAGGTAGAGATCCAGGTGAAGTATGAGGGCTATATCGCGCGCCAGCAGGATGAGATTGAG
AAACAACAGCGCAATGAGAATACCTTGCTGCCAGCGACCCTCGACTACCGCCAGGTCACAGGGCTGTCTAACGAGGTGAT
TGCCAAACTGAACGACCATAAACCGTCGTCGATAGGGCAGGCGTCACGTATCTCCGGGATCACCCCGGCGGCGATCTCTA
TTTTGCTGGTTTGGCTGAAAAAGCAGGGGATGCTGCGCCGCAGCGCCTGA

Upstream 100 bases:

>100_bases
CTCAGGATCCCGGAGTCTTTCTCGAAAGACGAAAGTTGAGTAGAATCCACGGCCCGGGCTTCAATCCATTTTCATACCGC
TTTATGCGAGGCAGACCACC

Downstream 100 bases:

>100_bases
TTGCGGCTGCCTGCCGCGCTGATTTCCCAGTTGCATAATCAGCGTTCGCCTTTATCATTCATAGCCCGGTAAGCGTTAGC
GCCTCCGGGCTCATTATTTT

Product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A [H]

Number of amino acids: Translated: 629; Mature: 629

Protein sequence:

>629_residues
MFYQDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQMSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQ
FRILNASKGPAVRATRAQADRVLYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVSRLKTGTPPRIDSRTIDFSVLAQQHGDNPMPVFSFMGSADQHPRQV
PCYITHTNEKTHDVIRNNLDRSPMYAGVIEGIGPRYCPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDV
QMQIVRSMQGMENARIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAARFSAEKEG
WAPRRDQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTEQGRELGLVDDERWARYNEKLESIERERQRL
KSTWVNPQAESANEVNAHLTAPLSREASGEDLLRRPEMTYEQLVQLSPFTPGLEDRQAAEQVEIQVKYEGYIARQQDEIE
KQQRNENTLLPATLDYRQVTGLSNEVIAKLNDHKPSSIGQASRISGITPAAISILLVWLKKQGMLRRSA

Sequences:

>Translated_629_residues
MFYQDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQMSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQ
FRILNASKGPAVRATRAQADRVLYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVSRLKTGTPPRIDSRTIDFSVLAQQHGDNPMPVFSFMGSADQHPRQV
PCYITHTNEKTHDVIRNNLDRSPMYAGVIEGIGPRYCPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDV
QMQIVRSMQGMENARIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAARFSAEKEG
WAPRRDQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTEQGRELGLVDDERWARYNEKLESIERERQRL
KSTWVNPQAESANEVNAHLTAPLSREASGEDLLRRPEMTYEQLVQLSPFTPGLEDRQAAEQVEIQVKYEGYIARQQDEIE
KQQRNENTLLPATLDYRQVTGLSNEVIAKLNDHKPSSIGQASRISGITPAAISILLVWLKKQGMLRRSA
>Mature_629_residues
MFYQDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQMSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQ
FRILNASKGPAVRATRAQADRVLYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVSRLKTGTPPRIDSRTIDFSVLAQQHGDNPMPVFSFMGSADQHPRQV
PCYITHTNEKTHDVIRNNLDRSPMYAGVIEGIGPRYCPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDV
QMQIVRSMQGMENARIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAARFSAEKEG
WAPRRDQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTEQGRELGLVDDERWARYNEKLESIERERQRL
KSTWVNPQAESANEVNAHLTAPLSREASGEDLLRRPEMTYEQLVQLSPFTPGLEDRQAAEQVEIQVKYEGYIARQQDEIE
KQQRNENTLLPATLDYRQVTGLSNEVIAKLNDHKPSSIGQASRISGITPAAISILLVWLKKQGMLRRSA

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34 [H]

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family [H]

Homologues:

Organism=Homo sapiens, GI74024895, Length=630, Percent_Identity=46.984126984127, Blast_Score=530, Evalue=1e-150,
Organism=Homo sapiens, GI19882217, Length=655, Percent_Identity=45.1908396946565, Blast_Score=515, Evalue=1e-146,
Organism=Homo sapiens, GI183227703, Length=670, Percent_Identity=44.1791044776119, Blast_Score=510, Evalue=1e-144,
Organism=Escherichia coli, GI2367273, Length=629, Percent_Identity=94.912559618442, Blast_Score=1228, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17534255, Length=642, Percent_Identity=40.6542056074766, Blast_Score=471, Evalue=1e-133,
Organism=Saccharomyces cerevisiae, GI6321202, Length=619, Percent_Identity=45.8804523424879, Blast_Score=521, Evalue=1e-148,
Organism=Drosophila melanogaster, GI24658174, Length=639, Percent_Identity=47.887323943662, Blast_Score=536, Evalue=1e-152,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595 [H]

Pfam domain/function: PF01134 GIDA [H]

EC number: NA

Molecular weight: Translated: 70117; Mature: 70117

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS50096 IQ ; PS01280 GIDA_1 ; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFYQDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQMSCNPAIGGIGKGHLV
CCCCCCCCEEEECCCCCCHHHHHHHHHCCCEEEEEEECCCHHHCCCCCCCCCCCCCCHHH
KEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRVLYRQAVRTALENQPNLMI
HHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCEEH
FQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLDGKIHIGLDNYSGGRAGDPPS
HHHHHHHHHHCCCCEEHHHHHHCHHHCCEEEEEEEECEECCEEEEEECCCCCCCCCCCCC
IPLSRRLRELPLRVSRLKTGTPPRIDSRTIDFSVLAQQHGDNPMPVFSFMGSADQHPRQV
CCHHHHHHHCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHCCCCCCCCCCC
PCYITHTNEKTHDVIRNNLDRSPMYAGVIEGIGPRYCPSIEDKVMRFADRNQHQIFLEPE
CEEEEECCCHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEEECCC
GLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENARIVRPGYAIEYDFFDPRDLKPTLESK
CCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEECCCEEEEECCCCCCCCCHHHHHH
FIQGLFFAGQINGTTGYEEAAAQGLLAGLNAARFSAEKEGWAPRRDQAYLGVLVDDLCTL
HHHHEEEEEEECCCCCHHHHHHHHHHHHCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHC
GTKEPYRMFTSRAEYRLMLREDNADLRLTEQGRELGLVDDERWARYNEKLESIERERQRL
CCCCHHHHHHCCCCEEEEEEECCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
KSTWVNPQAESANEVNAHLTAPLSREASGEDLLRRPEMTYEQLVQLSPFTPGLEDRQAAE
HHHCCCCCCCCCCCCCEEEECCCCCCCCCHHHHHCCCCCHHHHHHCCCCCCCCCHHHHHC
QVEIQVKYEGYIARQQDEIEKQQRNENTLLPATLDYRQVTGLSNEVIAKLNDHKPSSIGQ
EEEEEEEECCEEECCHHHHHHHHCCCCCCCCEECCHHHHCCCCHHHHHHCCCCCCCCCCC
ASRISGITPAAISILLVWLKKQGMLRRSA
HHHCCCCCHHHHHHHHHHHHHCCCHHCCC
>Mature Secondary Structure
MFYQDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQMSCNPAIGGIGKGHLV
CCCCCCCCEEEECCCCCCHHHHHHHHHCCCEEEEEEECCCHHHCCCCCCCCCCCCCCHHH
KEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRVLYRQAVRTALENQPNLMI
HHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCEEH
FQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLDGKIHIGLDNYSGGRAGDPPS
HHHHHHHHHHCCCCEEHHHHHHCHHHCCEEEEEEEECEECCEEEEEECCCCCCCCCCCCC
IPLSRRLRELPLRVSRLKTGTPPRIDSRTIDFSVLAQQHGDNPMPVFSFMGSADQHPRQV
CCHHHHHHHCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHCCCCCCCCCCC
PCYITHTNEKTHDVIRNNLDRSPMYAGVIEGIGPRYCPSIEDKVMRFADRNQHQIFLEPE
CEEEEECCCHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEEECCC
GLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENARIVRPGYAIEYDFFDPRDLKPTLESK
CCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEECCCEEEEECCCCCCCCCHHHHHH
FIQGLFFAGQINGTTGYEEAAAQGLLAGLNAARFSAEKEGWAPRRDQAYLGVLVDDLCTL
HHHHEEEEEEECCCCCHHHHHHHHHHHHCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHC
GTKEPYRMFTSRAEYRLMLREDNADLRLTEQGRELGLVDDERWARYNEKLESIERERQRL
CCCCHHHHHHCCCCEEEEEEECCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
KSTWVNPQAESANEVNAHLTAPLSREASGEDLLRRPEMTYEQLVQLSPFTPGLEDRQAAE
HHHCCCCCCCCCCCCCEEEECCCCCCCCCHHHHHCCCCCHHHHHHCCCCCCCCCHHHHHC
QVEIQVKYEGYIARQQDEIEKQQRNENTLLPATLDYRQVTGLSNEVIAKLNDHKPSSIGQ
EEEEEEEECCEEECCHHHHHHHHCCCCCCCCEECCHHHHCCCCHHHHHHCCCCCCCCCCC
ASRISGITPAAISILLVWLKKQGMLRRSA
HHHCCCCCHHHHHHHHHHHHHCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA