Click here to switch to the map view.

The map label for this gene is trmE [H]

Identifier: 238897217

GI number: 238897217

Start: 5217275

End: 5218639

Strand: Direct

Name: trmE [H]

Synonym: KP1_5488

Alternate gene names: 238897217

Gene position: 5217275-5218639 (Clockwise)

Preceding gene: 238897216

Following gene: 238897218

Centisome position: 99.4

GC content: 63.15

Gene sequence:

>1365_bases
ATGAGCCATAACGACACTATCGTCGCCCAGGCAACCCCTCCGGGACGCGGGGGTGTGGGCATCCTGCGTATCTCCGGCCT
TAAGGCGCGCGACGTCGCGCAGGCGGTGCTGGGCAAGCTGCCGAAGCCGCGCTATGCCGACTACCTGCCGTTCAACGACG
TTGACGGTACCCCGCTGGATCAGGGGATTGCGCTGTGGTTCCCCGGGCCGAACTCCTTTACCGGGGAAGATGTGCTTGAG
CTGCAGGGCCACGGCGGCCCGGTCATTCTCGACCTGCTGCTTAAACGTATTCTGACCCTGCCGGGCCTGCGCATCGCCAG
GCCGGGTGAGTTTTCCGAGCGCGCGTTCCTCAACGACAAGCTCGATCTGGCGCAGGCAGAGGCCATCGCCGACCTTATCG
ACGCCAGTTCAGAGCAGGCGGCGCGCTCGGCGCTGAATTCGCTGCAGGGGGCGTTCTCCGCGCGGGTGAATCATCTTGTG
GAAGCGCTCACTCACCTGCGCATCTACGTGGAAGCGGCCATTGACTTCCCGGATGAAGAAATCGATTTCCTCTCCGACGG
CAAAATTGAAGCCCAGCTGAACGAGGTGATGGCCGATCTCGACGCCGTCCGCGCGGAAGCTCGTCAGGGCAGCCTGCTGC
GCGAAGGGATGAAAGTGGTGATCGCCGGGCGGCCAAATGCCGGTAAATCAAGCCTGCTGAACGCCCTGGCGGGCCGCGAA
GCGGCGATCGTCACCGATATCGCCGGTACCACCCGCGACGTGCTACGCGAGCATATCCATATCGACGGCATGCCGCTGCA
TATCATCGATACCGCAGGCCTGCGCGATGCTAATGACGAAGTGGAACGTATCGGCATCGAGCGCGCCTGGCAGGAGATTG
CCCAGGCCGATCGGGTGCTGTTTATGGTCGATGGCACCACCACCGGCGCCGTTGACCCGGCGGAGATCTGGCCAGACTTT
ATCGAGCGCCTGCCCGCTAAACTGCCGATTACCGTGGTGCGCAATAAAGCCGATGTCACCGGCGAAGCGCTCGGTATCAG
CGAAGTAAATGGCCACTCACTTATTCGCCTGTCGGCACGTACCGGCGAAGGCGTGGAGGTGCTACGTAACCACCTCAAGC
AGAGCATGGGCTTCGATACCAATATGGAAGGCGGCTTCCTCGCCCGCCGCCGTCACCTGCAGGCCCTGGAAGAGGCGGCG
AACCATCTGCAGCAAGGCAAAGCCCAGCTGCTGGGCGCCTGGGCCGGCGAGCTGCTGGCGGAAGAGCTGCGCCTCGCGCA
GCAGGCGCTGAGCGAGATCACCGGGGAGTTCACTTCCGACGATCTGCTGGGCCGCATCTTCTCCAGCTTCTGCATCGGTA
AGTAA

Upstream 100 bases:

>100_bases
CGCATCTGCGACTTGGATAATTTAGAGTAGTTACGGTAACGTTAATGCCAGAGAAGGCGGTCAATGGACCGCCTTTTTTA
CATCTACATAGAGAGTCACC

Downstream 100 bases:

>100_bases
CCGCCAGTCCGTAAACGTTCACTAACCCGTATTAACGCCGCGTTAATACGGGTTTTTTCTTGCCCTTTGCGGCGCAACGT
TCTCTACTTTTATCTCCCCC

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 454; Mature: 453

Protein sequence:

>454_residues
MSHNDTIVAQATPPGRGGVGILRISGLKARDVAQAVLGKLPKPRYADYLPFNDVDGTPLDQGIALWFPGPNSFTGEDVLE
LQGHGGPVILDLLLKRILTLPGLRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNEVMADLDAVRAEARQGSLLREGMKVVIAGRPNAGKSSLLNALAGRE
AAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRDANDEVERIGIERAWQEIAQADRVLFMVDGTTTGAVDPAEIWPDF
IERLPAKLPITVVRNKADVTGEALGISEVNGHSLIRLSARTGEGVEVLRNHLKQSMGFDTNMEGGFLARRRHLQALEEAA
NHLQQGKAQLLGAWAGELLAEELRLAQQALSEITGEFTSDDLLGRIFSSFCIGK

Sequences:

>Translated_454_residues
MSHNDTIVAQATPPGRGGVGILRISGLKARDVAQAVLGKLPKPRYADYLPFNDVDGTPLDQGIALWFPGPNSFTGEDVLE
LQGHGGPVILDLLLKRILTLPGLRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNEVMADLDAVRAEARQGSLLREGMKVVIAGRPNAGKSSLLNALAGRE
AAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRDANDEVERIGIERAWQEIAQADRVLFMVDGTTTGAVDPAEIWPDF
IERLPAKLPITVVRNKADVTGEALGISEVNGHSLIRLSARTGEGVEVLRNHLKQSMGFDTNMEGGFLARRRHLQALEEAA
NHLQQGKAQLLGAWAGELLAEELRLAQQALSEITGEFTSDDLLGRIFSSFCIGK
>Mature_453_residues
SHNDTIVAQATPPGRGGVGILRISGLKARDVAQAVLGKLPKPRYADYLPFNDVDGTPLDQGIALWFPGPNSFTGEDVLEL
QGHGGPVILDLLLKRILTLPGLRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLVE
ALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNEVMADLDAVRAEARQGSLLREGMKVVIAGRPNAGKSSLLNALAGREA
AIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRDANDEVERIGIERAWQEIAQADRVLFMVDGTTTGAVDPAEIWPDFI
ERLPAKLPITVVRNKADVTGEALGISEVNGHSLIRLSARTGEGVEVLRNHLKQSMGFDTNMEGGFLARRRHLQALEEAAN
HLQQGKAQLLGAWAGELLAEELRLAQQALSEITGEFTSDDLLGRIFSSFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082991, Length=468, Percent_Identity=36.7521367521368, Blast_Score=249, Evalue=3e-66,
Organism=Homo sapiens, GI193082988, Length=500, Percent_Identity=33.8, Blast_Score=236, Evalue=3e-62,
Organism=Homo sapiens, GI193082993, Length=462, Percent_Identity=37.2294372294372, Blast_Score=234, Evalue=1e-61,
Organism=Escherichia coli, GI2367268, Length=454, Percent_Identity=94.273127753304, Blast_Score=861, Evalue=0.0,
Organism=Escherichia coli, GI87082120, Length=91, Percent_Identity=39.5604395604396, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17507259, Length=456, Percent_Identity=28.7280701754386, Blast_Score=167, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6323665, Length=492, Percent_Identity=30.6910569105691, Blast_Score=214, Evalue=3e-56,
Organism=Drosophila melanogaster, GI45550813, Length=483, Percent_Identity=32.0910973084886, Blast_Score=212, Evalue=4e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 49084; Mature: 48953

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHNDTIVAQATPPGRGGVGILRISGLKARDVAQAVLGKLPKPRYADYLPFNDVDGTPLD
CCCCCEEEEECCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
QGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTLPGLRIARPGEFSERAFLNDK
CCEEEEECCCCCCCCCCHHEECCCCCHHHHHHHHHHHHHCCCCEEECCCCCCHHHHCCCH
LDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLVEALTHLRIYVEAAIDFPDEE
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCH
IDFLSDGKIEAQLNEVMADLDAVRAEARQGSLLREGMKVVIAGRPNAGKSSLLNALAGRE
HCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHCCCEEEEECCCCCCHHHHHHHHHCCC
AAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRDANDEVERIGIERAWQEIAQADRVL
CEEEECCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCEE
FMVDGTTTGAVDPAEIWPDFIERLPAKLPITVVRNKADVTGEALGISEVNGHSLIRLSAR
EEEECCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCEECCCCEEEEEECC
TGEGVEVLRNHLKQSMGFDTNMEGGFLARRRHLQALEEAANHLQQGKAQLLGAWAGELLA
CCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EELRLAQQALSEITGEFTSDDLLGRIFSSFCIGK
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SHNDTIVAQATPPGRGGVGILRISGLKARDVAQAVLGKLPKPRYADYLPFNDVDGTPLD
CCCCEEEEECCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
QGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTLPGLRIARPGEFSERAFLNDK
CCEEEEECCCCCCCCCCHHEECCCCCHHHHHHHHHHHHHCCCCEEECCCCCCHHHHCCCH
LDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLVEALTHLRIYVEAAIDFPDEE
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCH
IDFLSDGKIEAQLNEVMADLDAVRAEARQGSLLREGMKVVIAGRPNAGKSSLLNALAGRE
HCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHCCCEEEEECCCCCCHHHHHHHHHCCC
AAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRDANDEVERIGIERAWQEIAQADRVL
CEEEECCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCEE
FMVDGTTTGAVDPAEIWPDFIERLPAKLPITVVRNKADVTGEALGISEVNGHSLIRLSAR
EEEECCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCEECCCCEEEEEECC
TGEGVEVLRNHLKQSMGFDTNMEGGFLARRRHLQALEEAANHLQQGKAQLLGAWAGELLA
CCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EELRLAQQALSEITGEFTSDDLLGRIFSSFCIGK
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA