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The map label for this gene is oprM [H]

Identifier: 238897145

GI number: 238897145

Start: 5144948

End: 5146312

Strand: Direct

Name: oprM [H]

Synonym: KP1_5408

Alternate gene names: 238897145

Gene position: 5144948-5146312 (Clockwise)

Preceding gene: 238897144

Following gene: 238897146

Centisome position: 98.03

GC content: 64.18

Gene sequence:

>1365_bases
ATGTTTCGTTTAAGTTTTATGTTTATCGCGCTCCTGACCGCGGGCTGCGTCTCGCTTGACCCGCATTATGATCGCCCGGC
CGCGCCGGTGCCGGCCACCCTGCCCGGGGCGCACGGCGAATCGACGGCGGTGGTCGGCGACTGGCAGAAAGTGGTCAATG
ACGCGCGGCTGAAAAAATTGGTCAGCATCGCGCTGAACAGCAACCGCGATGTGCAAAAGGCGCTGGCCGATATCGAGGCC
GCGCGCGCCCAGTATGGCGAAACGCGGGCATCGCTGTTTCCCACCGTTGATGCCGAACTGAGCCACACCCGCAGCAAAAC
GGTGGCCAGCGGCCTGAGCTCCGCTTCACAGGCCGACGGCGCGGTCTCCAGCTTCGAACTGGACTTGTTCGGCAAGAACC
AGAGCCTGTCGCGCGCGGCGCGCGAAACCTGGCTTGCCAGCGAATTCACCGCGCAGAATACCCGGCTGACGATGATTGCC
GATTTGACCACCGCCTGGGTGACGCTGGCGACGGATAACAGCAATCTGGCGCTGGCGCAGCAGACCATGGACAGCGCGGC
AAACTCGCGCAACATCGTCGCCCGCCAGATGGCGGTTGGCACCGCCTCAGCCGGCGATGTAAGCTCGGCGGAAAGCGTCT
ATCAGCAGGCGCGCGCCAGCGTCGCCAGCTATCGCACGCTGGTGGCGCAGGATAAAAACGCCATTAACCTGCTGGCGGGG
GAAACCGTGCCGGAGAGCCTGCTGCCCGGCACTCTCGAGAGTCTCGGGGATAACAGCATCGCGCTGGTGCCGGCGGGCGT
GTCGTCGTCGGTGCTGCTGCGTCGGCCGGACATTCAGGAAGCGGAGCACAATCTGAAGAGCGCCAATGCCGATATCGGCG
CGGCGCGGGCGAACTTCTTCCCGTCGATTTCCCTGACCGCCAGCGCCGGGGTCGGCAGCGACTCGCTGTCGTCGCTGTTC
AGCCACGGGATGCAGGTGTGGTCGTTCGCCCCCTCCATCAGCCTGCCGCTGTTCACCGGCGGCAGCAACCTGTCGCAGCT
GCGCTATGCGGAAGCGGAGAAAAAGGGGCTGATCGCCACCTACGAGAAATCGATTCAGAGCGCGTTTAAGGACGTTGCCG
ATGCGCTGGCGCGCCGGGAAACGCTGAGCGAAGAGCTTGATGCTCAGCGGCAGTATGTCGCCGCCGAGCAGACATCGCTG
GATATCGCCATGAAGAGCTATCAGGCCGGGGTCGGCGATTATCTGTCGGTGCTCACCGCTCAGCGCACGCTGTGGTCGGC
GAAAACGACGCTGCTGTCCCTGCAGCAGACCGACCTCAATAACCGGATCACCCTCTGGCAGTCGCTGGGCGGCGGCGCCA
GTTAA

Upstream 100 bases:

>100_bases
CACCCTGACGGCCACCCTGCTGGCGGTGTTCTTCGTACCGCTGTTCTTCGTGCTGGTGAAACGTCTCTTCACCCGCCAAC
GTCCTTCTCAGGAGTAAGGT

Downstream 100 bases:

>100_bases
ACTTACCCGCCGGGCGACCGAGCCCGGCCTCTACAAGGAATCGTTATGGCACGTGTTTCCCTCTCCTGGGCGCTGATCCT
CGGCCTGCTGTCGGGTATCG

Product: putative outer membrane efflux protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 454; Mature: 454

Protein sequence:

>454_residues
MFRLSFMFIALLTAGCVSLDPHYDRPAAPVPATLPGAHGESTAVVGDWQKVVNDARLKKLVSIALNSNRDVQKALADIEA
ARAQYGETRASLFPTVDAELSHTRSKTVASGLSSASQADGAVSSFELDLFGKNQSLSRAARETWLASEFTAQNTRLTMIA
DLTTAWVTLATDNSNLALAQQTMDSAANSRNIVARQMAVGTASAGDVSSAESVYQQARASVASYRTLVAQDKNAINLLAG
ETVPESLLPGTLESLGDNSIALVPAGVSSSVLLRRPDIQEAEHNLKSANADIGAARANFFPSISLTASAGVGSDSLSSLF
SHGMQVWSFAPSISLPLFTGGSNLSQLRYAEAEKKGLIATYEKSIQSAFKDVADALARRETLSEELDAQRQYVAAEQTSL
DIAMKSYQAGVGDYLSVLTAQRTLWSAKTTLLSLQQTDLNNRITLWQSLGGGAS

Sequences:

>Translated_454_residues
MFRLSFMFIALLTAGCVSLDPHYDRPAAPVPATLPGAHGESTAVVGDWQKVVNDARLKKLVSIALNSNRDVQKALADIEA
ARAQYGETRASLFPTVDAELSHTRSKTVASGLSSASQADGAVSSFELDLFGKNQSLSRAARETWLASEFTAQNTRLTMIA
DLTTAWVTLATDNSNLALAQQTMDSAANSRNIVARQMAVGTASAGDVSSAESVYQQARASVASYRTLVAQDKNAINLLAG
ETVPESLLPGTLESLGDNSIALVPAGVSSSVLLRRPDIQEAEHNLKSANADIGAARANFFPSISLTASAGVGSDSLSSLF
SHGMQVWSFAPSISLPLFTGGSNLSQLRYAEAEKKGLIATYEKSIQSAFKDVADALARRETLSEELDAQRQYVAAEQTSL
DIAMKSYQAGVGDYLSVLTAQRTLWSAKTTLLSLQQTDLNNRITLWQSLGGGAS
>Mature_454_residues
MFRLSFMFIALLTAGCVSLDPHYDRPAAPVPATLPGAHGESTAVVGDWQKVVNDARLKKLVSIALNSNRDVQKALADIEA
ARAQYGETRASLFPTVDAELSHTRSKTVASGLSSASQADGAVSSFELDLFGKNQSLSRAARETWLASEFTAQNTRLTMIA
DLTTAWVTLATDNSNLALAQQTMDSAANSRNIVARQMAVGTASAGDVSSAESVYQQARASVASYRTLVAQDKNAINLLAG
ETVPESLLPGTLESLGDNSIALVPAGVSSSVLLRRPDIQEAEHNLKSANADIGAARANFFPSISLTASAGVGSDSLSSLF
SHGMQVWSFAPSISLPLFTGGSNLSQLRYAEAEKKGLIATYEKSIQSAFKDVADALARRETLSEELDAQRQYVAAEQTSL
DIAMKSYQAGVGDYLSVLTAQRTLWSAKTTLLSLQQTDLNNRITLWQSLGGGAS

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=452, Percent_Identity=36.5044247787611, Blast_Score=277, Evalue=9e-76,
Organism=Escherichia coli, GI2367348, Length=424, Percent_Identity=24.5283018867925, Blast_Score=97, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 48145; Mature: 48145

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRLSFMFIALLTAGCVSLDPHYDRPAAPVPATLPGAHGESTAVVGDWQKVVNDARLKKL
CEEHHHHHHHHHHHCHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHH
VSIALNSNRDVQKALADIEAARAQYGETRASLFPTVDAELSHTRSKTVASGLSSASQADG
HHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCC
AVSSFELDLFGKNQSLSRAARETWLASEFTAQNTRLTMIADLTTAWVTLATDNSNLALAQ
CCEEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCEEEEEEEECCCCEEHHH
QTMDSAANSRNIVARQMAVGTASAGDVSSAESVYQQARASVASYRTLVAQDKNAINLLAG
HHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEC
ETVPESLLPGTLESLGDNSIALVPAGVSSSVLLRRPDIQEAEHNLKSANADIGAARANFF
CCCCHHHCCHHHHHCCCCCEEEEECCCCCCEEEECCCHHHHHHHHHHCCCCCCHHHHCCC
PSISLTASAGVGSDSLSSLFSHGMQVWSFAPSISLPLFTGGSNLSQLRYAEAEKKGLIAT
CCEEEEECCCCCCHHHHHHHHCCHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHCCCHHH
YEKSIQSAFKDVADALARRETLSEELDAQRQYVAAEQTSLDIAMKSYQAGVGDYLSVLTA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
QRTLWSAKTTLLSLQQTDLNNRITLWQSLGGGAS
HHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCC
>Mature Secondary Structure
MFRLSFMFIALLTAGCVSLDPHYDRPAAPVPATLPGAHGESTAVVGDWQKVVNDARLKKL
CEEHHHHHHHHHHHCHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHH
VSIALNSNRDVQKALADIEAARAQYGETRASLFPTVDAELSHTRSKTVASGLSSASQADG
HHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCC
AVSSFELDLFGKNQSLSRAARETWLASEFTAQNTRLTMIADLTTAWVTLATDNSNLALAQ
CCEEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCEEEEEEEECCCCEEHHH
QTMDSAANSRNIVARQMAVGTASAGDVSSAESVYQQARASVASYRTLVAQDKNAINLLAG
HHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEC
ETVPESLLPGTLESLGDNSIALVPAGVSSSVLLRRPDIQEAEHNLKSANADIGAARANFF
CCCCHHHCCHHHHHCCCCCEEEEECCCCCCEEEECCCHHHHHHHHHHCCCCCCHHHHCCC
PSISLTASAGVGSDSLSSLFSHGMQVWSFAPSISLPLFTGGSNLSQLRYAEAEKKGLIAT
CCEEEEECCCCCCHHHHHHHHCCHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHCCCHHH
YEKSIQSAFKDVADALARRETLSEELDAQRQYVAAEQTSLDIAMKSYQAGVGDYLSVLTA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
QRTLWSAKTTLLSLQQTDLNNRITLWQSLGGGAS
HHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]