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The map label for this gene is pagL [H]

Identifier: 238897118

GI number: 238897118

Start: 5115466

End: 5116845

Strand: Direct

Name: pagL [H]

Synonym: KP1_5376

Alternate gene names: 238897118

Gene position: 5115466-5116845 (Clockwise)

Preceding gene: 238897117

Following gene: 238897122

Centisome position: 97.46

GC content: 57.46

Gene sequence:

>1380_bases
ATGAAATTAACCGTATTAGGCGGGGGCGGCGTTCGCTCTGCATTTTTAGCGAAATCTCTGGCCTATAACGCTCACCGCAT
TGGTTTAACAGAAGTCGTTTTTCTCGATAACTCTGCTGATAATCTGGCGATATTTGGCGAAATTGCCCGCTATGTATTTA
ATACCATTCGCCCGGATATTCAGTTTAGTACCACCACCGACCCGGTCGCAGCGCTGCAGGACGCCAACTATATCATTACC
ACCCTGCGGGTTGGCGGCGATGAAAGCCGCATTCGCGACGAACGAATTGCGCTTGAGCATAACACTCTCGGCCAGGAAAC
CACCGGCGCCGGCGGTTTCGCGATGGCCATGCGCTCGATCCCGGCGATCCTTCGCTATTGCCGGCTGATCGAAGAACATG
CCGCCGAAGACGCTATTCTGTTTAACTTTACCAACCCGTCAGGTCTGGTGACGGAAGCGATTATTAAGTCCGGCTTTAAG
CGCCGGGTCTATGGAATCTGCGATGCCCCCTCCGAGCTGATTCGTGAACTGCCGGCGATCCTTGGCTGTGAGGAGCGCGA
CCTCAGCGTGGAATGCTATGGTCTGAACCATTTCTCCTGGTTTACCCACTTCACCGTCCGTGGGGAAGAGGTGACCGAAC
GGCTGATCGCCAGCCCGGAACTGTACCAGAAGACGGCGATGCAGTACTTCTCCCCGGAGCTGGTACGGCTTTGCGATAAT
CAGCTCCTCAATGAATATCTCTATTATTACTACTATCGCGACGAGGCGCTGAAGGCGATTCAGGGGGCCGGCGAGACGCG
CGGAGAGCAGATCGCCCGCATCAATCAGGAGATGCGCGAGGCGCTGCGCACCGTGGATGCCAGAACGCAACCGGAGGCCG
CCTTCACCATCTGGATGCAGCATTATCTGCGCCGGGAAAACAGCTATATGCAGAACGAATCCCGGCAGGAGAAATTCCAC
ACCCGCGAACCGCTGACGCTGCGCCAGTTTATCGAAGAGCCGGACACCGGCGGCTATGCCGGAGTGGCGCTGGATATCCT
GGAAGCGGTGAACAGCACCACCACCAAGCGGATCGTGGTGTCGATCCAGAATAATGGCACGCTCGATTTTTTACGCCCGG
ACGACGTGATCGAGATCAGCTGCGACCTGAGCCGGGATGGCCTCAAGCCGGTGACGCCGGTGAAGGTGCCGACAGCGCAG
AAGAATATGATTGCCTGCGTGAAGGAGTATGAGCGGCTGGCGGTGGCGGCGATCCTGCAGCAGGATAAATCGCTGGCGGT
GCGGGCGCTGATGGCGCACCCGCTGATCGGTTCCTATTCGCTGGCGAAAACCCTGGTCGAAGCCTACCTCGACGATGAGC
AGTTCGCCGCCTGGCGGTAA

Upstream 100 bases:

>100_bases
ACGCAATCGAAATGAAGTTCAGGTTATTTATGGCGTCAAAGTGGGACAGGTACGTTCCCGGGTTGATAACTGGTTAGCAG
AAAATTAATGAGGAGTATTT

Downstream 100 bases:

>100_bases
TCCGCTGCCGATGGGATTCCCCCGCTCGCACCGCGCTTAGCGGGGCTACGAACGGAGCGGTTTGTGTTTTATGTAGGCCG
GGTCAGGCGTAAGCCGCCAC

Product: putative glycoside hydrolase

Products: D-glucose; D-glucose 6-phosphate

Alternate protein names: NA

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MKLTVLGGGGVRSAFLAKSLAYNAHRIGLTEVVFLDNSADNLAIFGEIARYVFNTIRPDIQFSTTTDPVAALQDANYIIT
TLRVGGDESRIRDERIALEHNTLGQETTGAGGFAMAMRSIPAILRYCRLIEEHAAEDAILFNFTNPSGLVTEAIIKSGFK
RRVYGICDAPSELIRELPAILGCEERDLSVECYGLNHFSWFTHFTVRGEEVTERLIASPELYQKTAMQYFSPELVRLCDN
QLLNEYLYYYYYRDEALKAIQGAGETRGEQIARINQEMREALRTVDARTQPEAAFTIWMQHYLRRENSYMQNESRQEKFH
TREPLTLRQFIEEPDTGGYAGVALDILEAVNSTTTKRIVVSIQNNGTLDFLRPDDVIEISCDLSRDGLKPVTPVKVPTAQ
KNMIACVKEYERLAVAAILQQDKSLAVRALMAHPLIGSYSLAKTLVEAYLDDEQFAAWR

Sequences:

>Translated_459_residues
MKLTVLGGGGVRSAFLAKSLAYNAHRIGLTEVVFLDNSADNLAIFGEIARYVFNTIRPDIQFSTTTDPVAALQDANYIIT
TLRVGGDESRIRDERIALEHNTLGQETTGAGGFAMAMRSIPAILRYCRLIEEHAAEDAILFNFTNPSGLVTEAIIKSGFK
RRVYGICDAPSELIRELPAILGCEERDLSVECYGLNHFSWFTHFTVRGEEVTERLIASPELYQKTAMQYFSPELVRLCDN
QLLNEYLYYYYYRDEALKAIQGAGETRGEQIARINQEMREALRTVDARTQPEAAFTIWMQHYLRRENSYMQNESRQEKFH
TREPLTLRQFIEEPDTGGYAGVALDILEAVNSTTTKRIVVSIQNNGTLDFLRPDDVIEISCDLSRDGLKPVTPVKVPTAQ
KNMIACVKEYERLAVAAILQQDKSLAVRALMAHPLIGSYSLAKTLVEAYLDDEQFAAWR
>Mature_459_residues
MKLTVLGGGGVRSAFLAKSLAYNAHRIGLTEVVFLDNSADNLAIFGEIARYVFNTIRPDIQFSTTTDPVAALQDANYIIT
TLRVGGDESRIRDERIALEHNTLGQETTGAGGFAMAMRSIPAILRYCRLIEEHAAEDAILFNFTNPSGLVTEAIIKSGFK
RRVYGICDAPSELIRELPAILGCEERDLSVECYGLNHFSWFTHFTVRGEEVTERLIASPELYQKTAMQYFSPELVRLCDN
QLLNEYLYYYYYRDEALKAIQGAGETRGEQIARINQEMREALRTVDARTQPEAAFTIWMQHYLRRENSYMQNESRQEKFH
TREPLTLRQFIEEPDTGGYAGVALDILEAVNSTTTKRIVVSIQNNGTLDFLRPDDVIEISCDLSRDGLKPVTPVKVPTAQ
KNMIACVKEYERLAVAAILQQDKSLAVRALMAHPLIGSYSLAKTLVEAYLDDEQFAAWR

Specific function: Phospho-alpha-glucosidase that catalyzes the hydrolysis of p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate, but is not able to cleave 'natural' phospho-alpha-glucosides produced via the phosphoenolpyruvate-dependent sugar phosphotransferase system (PEP-

COG id: COG1486

COG function: function code G; Alpha-galactosidases/6-phospho-beta-glucosidases, family 4 of glycosyl hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 4 family [H]

Homologues:

Organism=Escherichia coli, GI1788029, Length=455, Percent_Identity=26.1538461538462, Blast_Score=148, Evalue=9e-37,
Organism=Escherichia coli, GI1790560, Length=483, Percent_Identity=21.7391304347826, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019802
- InterPro:   IPR001088
- InterPro:   IPR022616
- InterPro:   IPR015955
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02056 Glyco_hydro_4; PF11975 Glyco_hydro_4C [H]

EC number: 3.2.1.86

Molecular weight: Translated: 51739; Mature: 51739

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLTVLGGGGVRSAFLAKSLAYNAHRIGLTEVVFLDNSADNLAIFGEIARYVFNTIRPDI
CEEEEECCCCHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCE
QFSTTTDPVAALQDANYIITTLRVGGDESRIRDERIALEHNTLGQETTGAGGFAMAMRSI
EECCCCCHHHHHCCCCEEEEEEEECCCHHHHHHHHHEEECCCCCCCCCCCCHHHHHHHHH
PAILRYCRLIEEHAAEDAILFNFTNPSGLVTEAIIKSGFKRRVYGICDAPSELIRELPAI
HHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCHHHHEEECCCCHHHHHHHHHHH
LGCEERDLSVECYGLNHFSWFTHFTVRGEEVTERLIASPELYQKTAMQYFSPELVRLCDN
HCCCCCCCEEEEEECCCEEEEEEEEEECHHHHHHHHCCHHHHHHHHHHHHCHHHHHHHHH
QLLNEYLYYYYYRDEALKAIQGAGETRGEQIARINQEMREALRTVDARTQPEAAFTIWMQ
HHHHHHHEEEEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
HYLRRENSYMQNESRQEKFHTREPLTLRQFIEEPDTGGYAGVALDILEAVNSTTTKRIVV
HHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCEEEEEE
SIQNNGTLDFLRPDDVIEISCDLSRDGLKPVTPVKVPTAQKNMIACVKEYERLAVAAILQ
EEECCCEEEEECCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
QDKSLAVRALMAHPLIGSYSLAKTLVEAYLDDEQFAAWR
CCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHCCCC
>Mature Secondary Structure
MKLTVLGGGGVRSAFLAKSLAYNAHRIGLTEVVFLDNSADNLAIFGEIARYVFNTIRPDI
CEEEEECCCCHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCE
QFSTTTDPVAALQDANYIITTLRVGGDESRIRDERIALEHNTLGQETTGAGGFAMAMRSI
EECCCCCHHHHHCCCCEEEEEEEECCCHHHHHHHHHEEECCCCCCCCCCCCHHHHHHHHH
PAILRYCRLIEEHAAEDAILFNFTNPSGLVTEAIIKSGFKRRVYGICDAPSELIRELPAI
HHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCHHHHEEECCCCHHHHHHHHHHH
LGCEERDLSVECYGLNHFSWFTHFTVRGEEVTERLIASPELYQKTAMQYFSPELVRLCDN
HCCCCCCCEEEEEECCCEEEEEEEEEECHHHHHHHHCCHHHHHHHHHHHHCHHHHHHHHH
QLLNEYLYYYYYRDEALKAIQGAGETRGEQIARINQEMREALRTVDARTQPEAAFTIWMQ
HHHHHHHEEEEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
HYLRRENSYMQNESRQEKFHTREPLTLRQFIEEPDTGGYAGVALDILEAVNSTTTKRIVV
HHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCEEEEEE
SIQNNGTLDFLRPDDVIEISCDLSRDGLKPVTPVKVPTAQKNMIACVKEYERLAVAAILQ
EEECCCEEEEECCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
QDKSLAVRALMAHPLIGSYSLAKTLVEAYLDDEQFAAWR
CCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.18 {p-nitrophenyl-beta-D-glucopyranoside-6-phosphate}} 0.46 {phenyl} 0.5 {salicin} 0.19 {methyl} [C]

Substrates: 6-phospho-beta-D-glucosyl-(1,4)-D-glucose; H2O

Specific reaction: 6-phospho-beta-D-glucosyl-(1,4)-D-glucose + H2O = D-glucose + D-glucose 6-phosphate

General reaction: O-Glycosyl bond hydrolysis [C]

Inhibitor: AMP; Phosphoenolpyruvate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]