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The map label for this gene is intS [H]

Identifier: 238897122

GI number: 238897122

Start: 5122188

End: 5123363

Strand: Direct

Name: intS [H]

Synonym: KP1_5380

Alternate gene names: 238897122

Gene position: 5122188-5123363 (Clockwise)

Preceding gene: 238897118

Following gene: 238897123

Centisome position: 97.59

GC content: 45.41

Gene sequence:

>1176_bases
ATGGCACTTACAGATATACAAATCAAACGAGCAAAGCCCCAAGAAAAGCCATATACATTGAACGATGGGCAAGGCCTGTC
GTTGCTTATCAACCCCGATGGCTCGAAGGGCTGGCGGTTCCGTTTCCGCCTTGCTGGGAAAGCGCGGTTAATGTCATTTG
GTAGCTACGACCTTGTGAGCCTCGCAGAAGCACGTGAGAAACGCGAGACGTCCCGTAAGCAGGTTGCAAATGGCATTGAC
CCCGTTGAGGAACGCAAATCACAAAAACTCGCTCAGAAGCTATCAACAGAAAACTCTTTCGAAGCCATATGTCGAGAATG
GCACACTAACAAAGCTGACCGCTGGACAGTGGCCTATCGCGAAGAAATCATTAAGACATTCGAGAAAGATGTGTTCCCGT
TCATTGGTAAACGCCCTAACAGTGAAATCAAGCCGTTAGAACTGCTTGAAGTATTGCGGCGAATAGAGAAGCGTGGAGCG
CTGGAGAAAACACGCAAAGTGCGCCAAAGATGTGGCGAGGTTTATCGATATGCAATTATAACTGGCCGCGCTGAATATAA
CCCAGCACCTGATTTAGCTATCGCCTTGGCCGTTCCTAAGCAGAAGCACCACCCTTTTTTATCTGCCGAAGAATTACCTC
ATTTTATTCGTGATCTGGAAGCGTACACCGGTAGCATCATCACCAAAAATGCTACAAAGATAATCATGCTAACTGGTGTA
AGAACGCAGGAGATGCGCTTTGCTACGGGGGATGAAATTGATTTAGAAAAGGCAATTTGGGAAATCCCAGCAGAACGGAT
GAAAATGCGTAGGCCGCATATTGTTCCTTTATCTACCCAGGTCATTGACCTCTTTAGGCAACTCAAGCCTATTACCGGTC
ATTATCCTTATATTTTTATCGGCAGAAAAAACCGCAGTAAGCCAATTTCGAAAGAAAGTGTCTCTCAAGTGATTGAGTTA
CTTGGCTACAAAGGCCGTGCCACAGGTCACGGATTCAGACATACAATGTCCACTATCCTGCATGAGCAGGGATTTGATAG
TGCATGGATTGAAATGCAATTAGCGCACGTTGATAAAAATAAAATTCGCGGCATTTACAATCATGCTCAATACATAGAAG
ATCGAACGAAAATGATGCAGTGGTATAGTGATCTTTTCACTGAGCCCAAAAGGTGA

Upstream 100 bases:

>100_bases
ATGACTCATCCGTAACCGTCCGACAAGTTCTCACTGAATCCAAGATAAATATGTATAGTGATGTGTATAGATTTTTTATA
CACATTTTTGGCTTATACAC

Downstream 100 bases:

>100_bases
AAATAATGAGTGCAAGTTTAGATGATGTAAAAGATTTTGACTTTGTGATCTCAGAAGCATTTAGAATAGATGTTGGATTA
GATTCACCTAATTTTAGAGA

Product: phage integrase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 391; Mature: 390

Protein sequence:

>391_residues
MALTDIQIKRAKPQEKPYTLNDGQGLSLLINPDGSKGWRFRFRLAGKARLMSFGSYDLVSLAEAREKRETSRKQVANGID
PVEERKSQKLAQKLSTENSFEAICREWHTNKADRWTVAYREEIIKTFEKDVFPFIGKRPNSEIKPLELLEVLRRIEKRGA
LEKTRKVRQRCGEVYRYAIITGRAEYNPAPDLAIALAVPKQKHHPFLSAEELPHFIRDLEAYTGSIITKNATKIIMLTGV
RTQEMRFATGDEIDLEKAIWEIPAERMKMRRPHIVPLSTQVIDLFRQLKPITGHYPYIFIGRKNRSKPISKESVSQVIEL
LGYKGRATGHGFRHTMSTILHEQGFDSAWIEMQLAHVDKNKIRGIYNHAQYIEDRTKMMQWYSDLFTEPKR

Sequences:

>Translated_391_residues
MALTDIQIKRAKPQEKPYTLNDGQGLSLLINPDGSKGWRFRFRLAGKARLMSFGSYDLVSLAEAREKRETSRKQVANGID
PVEERKSQKLAQKLSTENSFEAICREWHTNKADRWTVAYREEIIKTFEKDVFPFIGKRPNSEIKPLELLEVLRRIEKRGA
LEKTRKVRQRCGEVYRYAIITGRAEYNPAPDLAIALAVPKQKHHPFLSAEELPHFIRDLEAYTGSIITKNATKIIMLTGV
RTQEMRFATGDEIDLEKAIWEIPAERMKMRRPHIVPLSTQVIDLFRQLKPITGHYPYIFIGRKNRSKPISKESVSQVIEL
LGYKGRATGHGFRHTMSTILHEQGFDSAWIEMQLAHVDKNKIRGIYNHAQYIEDRTKMMQWYSDLFTEPKR
>Mature_390_residues
ALTDIQIKRAKPQEKPYTLNDGQGLSLLINPDGSKGWRFRFRLAGKARLMSFGSYDLVSLAEAREKRETSRKQVANGIDP
VEERKSQKLAQKLSTENSFEAICREWHTNKADRWTVAYREEIIKTFEKDVFPFIGKRPNSEIKPLELLEVLRRIEKRGAL
EKTRKVRQRCGEVYRYAIITGRAEYNPAPDLAIALAVPKQKHHPFLSAEELPHFIRDLEAYTGSIITKNATKIIMLTGVR
TQEMRFATGDEIDLEKAIWEIPAERMKMRRPHIVPLSTQVIDLFRQLKPITGHYPYIFIGRKNRSKPISKESVSQVIELL
GYKGRATGHGFRHTMSTILHEQGFDSAWIEMQLAHVDKNKIRGIYNHAQYIEDRTKMMQWYSDLFTEPKR

Specific function: Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI1788690, Length=387, Percent_Identity=45.7364341085271, Blast_Score=367, Evalue=1e-103,
Organism=Escherichia coli, GI145693166, Length=385, Percent_Identity=40.5194805194805, Blast_Score=294, Evalue=7e-81,
Organism=Escherichia coli, GI1788974, Length=386, Percent_Identity=38.0829015544041, Blast_Score=264, Evalue=7e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR023109 [H]

Pfam domain/function: PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 45381; Mature: 45250

Theoretical pI: Translated: 10.30; Mature: 10.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALTDIQIKRAKPQEKPYTLNDGQGLSLLINPDGSKGWRFRFRLAGKARLMSFGSYDLVS
CCCCCCEEECCCCCCCCEEECCCCCEEEEECCCCCCCCEEEEEECCCHHHHCCCCCHHHH
LAEAREKRETSRKQVANGIDPVEERKSQKLAQKLSTENSFEAICREWHTNKADRWTVAYR
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHH
EEIIKTFEKDVFPFIGKRPNSEIKPLELLEVLRRIEKRGALEKTRKVRQRCGEVYRYAII
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHEE
TGRAEYNPAPDLAIALAVPKQKHHPFLSAEELPHFIRDLEAYTGSIITKNATKIIMLTGV
ECCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCEEECCCCEEEEEECC
RTQEMRFATGDEIDLEKAIWEIPAERMKMRRPHIVPLSTQVIDLFRQLKPITGHYPYIFI
CHHHEEECCCCCCHHHHHHHHCCHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEE
GRKNRSKPISKESVSQVIELLGYKGRATGHGFRHTMSTILHEQGFDSAWIEMQLAHVDKN
ECCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCHHHEEEHHHCCHH
KIRGIYNHAQYIEDRTKMMQWYSDLFTEPKR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
ALTDIQIKRAKPQEKPYTLNDGQGLSLLINPDGSKGWRFRFRLAGKARLMSFGSYDLVS
CCCCCEEECCCCCCCCEEECCCCCEEEEECCCCCCCCEEEEEECCCHHHHCCCCCHHHH
LAEAREKRETSRKQVANGIDPVEERKSQKLAQKLSTENSFEAICREWHTNKADRWTVAYR
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHH
EEIIKTFEKDVFPFIGKRPNSEIKPLELLEVLRRIEKRGALEKTRKVRQRCGEVYRYAII
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHEE
TGRAEYNPAPDLAIALAVPKQKHHPFLSAEELPHFIRDLEAYTGSIITKNATKIIMLTGV
ECCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCEEECCCCEEEEEECC
RTQEMRFATGDEIDLEKAIWEIPAERMKMRRPHIVPLSTQVIDLFRQLKPITGHYPYIFI
CHHHEEECCCCCCHHHHHHHHCCHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEE
GRKNRSKPISKESVSQVIELLGYKGRATGHGFRHTMSTILHEQGFDSAWIEMQLAHVDKN
ECCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCHHHEEEHHHCCHH
KIRGIYNHAQYIEDRTKMMQWYSDLFTEPKR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]