Click here to switch to the map view.

The map label for this gene is spoT [H]

Identifier: 238897100

GI number: 238897100

Start: 5093313

End: 5095433

Strand: Direct

Name: spoT [H]

Synonym: KP1_5353

Alternate gene names: 238897100

Gene position: 5093313-5095433 (Clockwise)

Preceding gene: 238897099

Following gene: 238897101

Centisome position: 97.04

GC content: 57.14

Gene sequence:

>2121_bases
TTGTATCTGTTTGAAAGCCTGAATCAGCTGATTCAAAACTACCTGCCGGAAGACCAGATTAAGCGTCTGCGGCAGGCGTA
TCTCGTTGCACGTGACGCTCACGAGGGTCAGACACGTTCAAGCGGTGAACCCTATATCACGCACCCGGTCGCGGTAGCCT
GCATTCTGGCCGAGATGAAGCTCGACTATGAAACGCTGATGGCCGCGCTGCTGCATGATGTGATTGAAGATACCCCCGCC
ACCTACCAGGACATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGACAAGCTCAA
GTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAAATGATCATGGCGATGGTGCAGGACATCCGCGTCATCC
TCATCAAGCTTGCCGACCGCACCCACAACATGCGTACTCTGGGCTCGCTGCGCCCGGATAAACGCCGACGCATCGCCCGC
GAAACGCTGGAAATTTACAGCCCGCTGGCGCACCGTTTAGGTATCCATCACATCAAAACCGAGCTCGAAGAGCTGGGCTT
TGAAGCGCTGTACCCTAATCGCTACCGCGTCATTAAAGAGGTGGTCAAAGCGGCGCGCGGCAACCGTAAGGAGATGATTC
AAAAGATCCTCTCCGAAATCGAAGGGCGTTTGCAGGAAGCGGGGATCCCCTGCCGCGTCAGCGGTCGCGAGAAGCATCTA
TACTCCATCTACTGTAAAATGGTGCTCAAAGAGCAACGTTTTCACTCGATCATGGATATCTACGCCTTCCGCGTTATCGT
CCATGATGCCGATATCTGCTATCGCGTGCTCGGCCAGATGCACAGCCTCTACAAACCGCGTCCGGGTCGTTTTAAAGACT
ACATCGCCATTCCGAAAGCTAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCCCACGGCGTCCCGGTCGAGGTA
CAAATTCGTACCGAAGACATGGACCAGATGGCGGAGATGGGGGTAGCGGCGCACTGGGCTTATAAAGAGCACGGCGGCGA
AAGCAGCACCACCGCGCAAATCCGCGCTCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAGCAGAGCGCCGGTAGCTCGT
TTGAATTTATCGAGAGCGTAAAATCCGATCTGTTCCCCGATGAGATTTACGTTTTCACCCCGGAAGGGCGCATTGTCGAA
CTGCCTGCCGGCGCCACGCCGGTGGACTTCGCCTACGCGGTGCATACCGATATCGGTCATGCCTGCGTCGGCGCGCGCGT
CGACCGTCAGCCCTATCCGCTGTCGCAGCCGCTCTCCAGCGGTCAGACTGTCGAAATTATCACCGCGCCGGGCGCTCGCC
CGAACGCCGCCTGGCTGAACTTTGTCGTCAGCTCGAAAGCGCGCGCCAAGATCCGTCAGCTGCTGAAAAACCTTAAGCGC
GACGATTCGGTTAGCCTTGGCCGTCGTCTGCTCAATCACGCGCTGGGCGGAAGCCGCAAACTTGCCGAGATCCCACCGGA
GAATATCCAGCGCGAACTCGATCGCATGAAGCTGGCCTCGCTTGACGATCTGCTGGCGGAAATCGGTCTGGGCAACGCCA
TGAGCGTCGTGGTGGCAAAAAACCTGCAGCAGGGCGAAGCCGCTGCGGCGCCGGTACCGGCTAACGCGTCGAACCACGGT
CATCTGCCAATCAAAGGCGCCGATGGCGTGTTGATCACCTTCGCCAAGTGCTGTCGTCCAATTCCAGGTGACCCGATTAT
CGCCCACGTCAGCCCGGGTAAAGGGCTGGTTATCCACCATGAATCCTGTCGTAACATTCGCGGCTACCAGAAAGAGCCGG
AGAAATTTATGGCGGTTGAGTGGGATAAAGAGACCGCTCAGGAGTTCATCACCGAAATCAAGGTGGACATGTTTAACCAC
CAGGGCGCGCTGGCTAACCTGACGGCGGCGATCAACACCGCGTCTTCCAATATCCAGAGCCTGAATACGGAAGAGAAAGA
TGGCCGCGTATACAGCGCCTTTATCCGCCTGACCGCGCGCGACCGCGTGCATCTGGCGAATATCATGCGCAAAATCCGCG
TCATGCCGGACGTGATTAAAGTCACCCGTAACCGAAACTAG

Upstream 100 bases:

>100_bases
CGTACGTGAGCGCCAGGAACAGCAAGAGCAGGAAGCCGCTGAATTACAAGCCGTTACCGCGATTGCTGAAGGTCGTCGTT
AATCACACAGCGGGTCGCCC

Downstream 100 bases:

>100_bases
CTTTATGAACTCAAAGCGCTACGAGCGTATCTGCGAGATGCTCGCCAGGCGTCAGCCTGACCTGACGGTCTGCATGGAGC
AGGTCCATAAGCCTCATAAC

Product: bifunctional (p)ppGpp synthetase II/ guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 706; Mature: 706

Protein sequence:

>706_residues
MYLFESLNQLIQNYLPEDQIKRLRQAYLVARDAHEGQTRSSGEPYITHPVAVACILAEMKLDYETLMAALLHDVIEDTPA
TYQDMEQLFGKSVAELVEGVSKLDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILSEIEGRLQEAGIPCRVSGREKHL
YSIYCKMVLKEQRFHSIMDIYAFRVIVHDADICYRVLGQMHSLYKPRPGRFKDYIAIPKANGYQSLHTSMIGPHGVPVEV
QIRTEDMDQMAEMGVAAHWAYKEHGGESSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFTPEGRIVE
LPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLSSGQTVEIITAPGARPNAAWLNFVVSSKARAKIRQLLKNLKR
DDSVSLGRRLLNHALGGSRKLAEIPPENIQRELDRMKLASLDDLLAEIGLGNAMSVVVAKNLQQGEAAAAPVPANASNHG
HLPIKGADGVLITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQEFITEIKVDMFNH
QGALANLTAAINTASSNIQSLNTEEKDGRVYSAFIRLTARDRVHLANIMRKIRVMPDVIKVTRNRN

Sequences:

>Translated_706_residues
MYLFESLNQLIQNYLPEDQIKRLRQAYLVARDAHEGQTRSSGEPYITHPVAVACILAEMKLDYETLMAALLHDVIEDTPA
TYQDMEQLFGKSVAELVEGVSKLDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILSEIEGRLQEAGIPCRVSGREKHL
YSIYCKMVLKEQRFHSIMDIYAFRVIVHDADICYRVLGQMHSLYKPRPGRFKDYIAIPKANGYQSLHTSMIGPHGVPVEV
QIRTEDMDQMAEMGVAAHWAYKEHGGESSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFTPEGRIVE
LPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLSSGQTVEIITAPGARPNAAWLNFVVSSKARAKIRQLLKNLKR
DDSVSLGRRLLNHALGGSRKLAEIPPENIQRELDRMKLASLDDLLAEIGLGNAMSVVVAKNLQQGEAAAAPVPANASNHG
HLPIKGADGVLITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQEFITEIKVDMFNH
QGALANLTAAINTASSNIQSLNTEEKDGRVYSAFIRLTARDRVHLANIMRKIRVMPDVIKVTRNRN
>Mature_706_residues
MYLFESLNQLIQNYLPEDQIKRLRQAYLVARDAHEGQTRSSGEPYITHPVAVACILAEMKLDYETLMAALLHDVIEDTPA
TYQDMEQLFGKSVAELVEGVSKLDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILSEIEGRLQEAGIPCRVSGREKHL
YSIYCKMVLKEQRFHSIMDIYAFRVIVHDADICYRVLGQMHSLYKPRPGRFKDYIAIPKANGYQSLHTSMIGPHGVPVEV
QIRTEDMDQMAEMGVAAHWAYKEHGGESSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFTPEGRIVE
LPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLSSGQTVEIITAPGARPNAAWLNFVVSSKARAKIRQLLKNLKR
DDSVSLGRRLLNHALGGSRKLAEIPPENIQRELDRMKLASLDDLLAEIGLGNAMSVVVAKNLQQGEAAAAPVPANASNHG
HLPIKGADGVLITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQEFITEIKVDMFNH
QGALANLTAAINTASSNIQSLNTEEKDGRVYSAFIRLTARDRVHLANIMRKIRVMPDVIKVTRNRN

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into G

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Escherichia coli, GI1790082, Length=706, Percent_Identity=96.3172804532578, Blast_Score=1410, Evalue=0.0,
Organism=Escherichia coli, GI1789147, Length=683, Percent_Identity=32.796486090776, Blast_Score=386, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI25143118, Length=139, Percent_Identity=33.0935251798561, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24650996, Length=118, Percent_Identity=40.6779661016949, Blast_Score=75, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 79494; Mature: 79494

Theoretical pI: Translated: 9.00; Mature: 9.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYLFESLNQLIQNYLPEDQIKRLRQAYLVARDAHEGQTRSSGEPYITHPVAVACILAEMK
CCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEHHHHHHHHHHHHH
LDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSKLDKLKFRDKKEAQAENFR
CCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
KMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIARETLEIYSPLAHRLGIHHIKT
HHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILSEIEGRLQEAGIPCRVSGREKHL
HHHHCCCHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHH
YSIYCKMVLKEQRFHSIMDIYAFRVIVHDADICYRVLGQMHSLYKPRPGRFKDYIAIPKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCC
NGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMGVAAHWAYKEHGGESSTTAQIRAQRWM
CCHHHHHHHHCCCCCCCEEEEEECCCHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHH
QSLLELQQSAGSSFEFIESVKSDLFPDEIYVFTPEGRIVELPAGATPVDFAYAVHTDIGH
HHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEECCCCCEEECCCCCCCCHHHEEHHCCCCH
ACVGARVDRQPYPLSQPLSSGQTVEIITAPGARPNAAWLNFVVSSKARAKIRQLLKNLKR
HHHCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
DDSVSLGRRLLNHALGGSRKLAEIPPENIQRELDRMKLASLDDLLAEIGLGNAMSVVVAK
CCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
NLQQGEAAAAPVPANASNHGHLPIKGADGVLITFAKCCRPIPGDPIIAHVSPGKGLVIHH
CCCCCCCCCCCCCCCCCCCCCCEECCCCCHHHHHHHHCCCCCCCCEEEEECCCCCEEEEC
ESCRNIRGYQKEPEKFMAVEWDKETAQEFITEIKVDMFNHQGALANLTAAINTASSNIQS
HHHCCCCCCCCCCCHHEEEECCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
LNTEEKDGRVYSAFIRLTARDRVHLANIMRKIRVMPDVIKVTRNRN
CCCCCCCCHHEEHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MYLFESLNQLIQNYLPEDQIKRLRQAYLVARDAHEGQTRSSGEPYITHPVAVACILAEMK
CCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEHHHHHHHHHHHHH
LDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSKLDKLKFRDKKEAQAENFR
CCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
KMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIARETLEIYSPLAHRLGIHHIKT
HHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILSEIEGRLQEAGIPCRVSGREKHL
HHHHCCCHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHH
YSIYCKMVLKEQRFHSIMDIYAFRVIVHDADICYRVLGQMHSLYKPRPGRFKDYIAIPKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCC
NGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMGVAAHWAYKEHGGESSTTAQIRAQRWM
CCHHHHHHHHCCCCCCCEEEEEECCCHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHH
QSLLELQQSAGSSFEFIESVKSDLFPDEIYVFTPEGRIVELPAGATPVDFAYAVHTDIGH
HHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEECCCCCEEECCCCCCCCHHHEEHHCCCCH
ACVGARVDRQPYPLSQPLSSGQTVEIITAPGARPNAAWLNFVVSSKARAKIRQLLKNLKR
HHHCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
DDSVSLGRRLLNHALGGSRKLAEIPPENIQRELDRMKLASLDDLLAEIGLGNAMSVVVAK
CCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
NLQQGEAAAAPVPANASNHGHLPIKGADGVLITFAKCCRPIPGDPIIAHVSPGKGLVIHH
CCCCCCCCCCCCCCCCCCCCCCEECCCCCHHHHHHHHCCCCCCCCEEEEECCCCCEEEEC
ESCRNIRGYQKEPEKFMAVEWDKETAQEFITEIKVDMFNHQGALANLTAAINTASSNIQS
HHHCCCCCCCCCCCHHEEEECCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
LNTEEKDGRVYSAFIRLTARDRVHLANIMRKIRVMPDVIKVTRNRN
CCCCCCCCHHEEHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]