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The map label for this gene is trmH [H]

Identifier: 238897101

GI number: 238897101

Start: 5095438

End: 5096127

Strand: Direct

Name: trmH [H]

Synonym: KP1_5354

Alternate gene names: 238897101

Gene position: 5095438-5096127 (Clockwise)

Preceding gene: 238897100

Following gene: 238897102

Centisome position: 97.08

GC content: 61.16

Gene sequence:

>690_bases
ATGAACTCAAAGCGCTACGAGCGTATCTGCGAGATGCTCGCCAGGCGTCAGCCTGACCTGACGGTCTGCATGGAGCAGGT
CCATAAGCCTCATAACGTCTCTGCGGTCATCCGGACCGCAGATGCCGTTGGCGTACACGAAGTGCATGCCGTCTGGCCGA
GCAGCCGGATGCGTACCATGGCCTCCGCCGCCGCGGGCAGCAACAGCTGGGTGCAGGTTAAAACCCACCGCACTATCGCA
GATGCCGTCGGCCACCTGAAAGGCCAGGGCATGCAAATTCTCGCCACTCACCTTTCCGACAAAGCCATCGATTTTCGTGA
AATCGACTATACGCGTCCAACCTGCATCCTGATGGGCCAGGAGAAAACCGGCATCACCCAGGAAGCGCTGGCGCTGGCCG
ATCAGGACATCATTATCCCGATGATCGGCATGGTGCAGTCGCTGAACGTCTCCGTCGCCTCGGCGCTGATCCTCTACGAG
GCGCAGCGCCAGCGGCAGAATGCCGGGATGTACCAACGCGCCAACAGCATGCTGCCGCCACAAGAGCAGCAGCGCCTGCT
GTTCGAAGGCGGCTATCCGGTGCTGGCCCGTGTCGCCAGACAAAAAGGTCTGCCTTACCCCCACGTTAACGAGCAGGGCG
AAGTGGAAGCCGATGCCGCGTGGTGGGCTACCATGCAGGCTGCGAGGTAA

Upstream 100 bases:

>100_bases
CGCCTGACCGCGCGCGACCGCGTGCATCTGGCGAATATCATGCGCAAAATCCGCGTCATGCCGGACGTGATTAAAGTCAC
CCGTAACCGAAACTAGCTTT

Downstream 100 bases:

>100_bases
ACCCAATGTCAGGCCGCCTGTTAGATGCCGTTCCGCTCAGTTCACTCACCGGTGTGGGCGCTGCGCAGAGCAGCAAACTG
GCGAAGATTGGCCTGCATAC

Product: tRNA guanosine-2'-O-methyltransferase

Products: NA

Alternate protein names: tRNA [Gm18] methyltransferase [H]

Number of amino acids: Translated: 229; Mature: 229

Protein sequence:

>229_residues
MNSKRYERICEMLARRQPDLTVCMEQVHKPHNVSAVIRTADAVGVHEVHAVWPSSRMRTMASAAAGSNSWVQVKTHRTIA
DAVGHLKGQGMQILATHLSDKAIDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
AQRQRQNAGMYQRANSMLPPQEQQRLLFEGGYPVLARVARQKGLPYPHVNEQGEVEADAAWWATMQAAR

Sequences:

>Translated_229_residues
MNSKRYERICEMLARRQPDLTVCMEQVHKPHNVSAVIRTADAVGVHEVHAVWPSSRMRTMASAAAGSNSWVQVKTHRTIA
DAVGHLKGQGMQILATHLSDKAIDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
AQRQRQNAGMYQRANSMLPPQEQQRLLFEGGYPVLARVARQKGLPYPHVNEQGEVEADAAWWATMQAAR
>Mature_229_residues
MNSKRYERICEMLARRQPDLTVCMEQVHKPHNVSAVIRTADAVGVHEVHAVWPSSRMRTMASAAAGSNSWVQVKTHRTIA
DAVGHLKGQGMQILATHLSDKAIDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
AQRQRQNAGMYQRANSMLPPQEQQRLLFEGGYPVLARVARQKGLPYPHVNEQGEVEADAAWWATMQAAR

Specific function: Specifically methylates guanosine-18 in various tRNAs [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1790083, Length=228, Percent_Identity=91.6666666666667, Blast_Score=439, Evalue=1e-125,
Organism=Escherichia coli, GI1790623, Length=160, Percent_Identity=32.5, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022724
- InterPro:   IPR001537 [H]

Pfam domain/function: PF12105 SpoU_methylas_C; PF00588 SpoU_methylase [H]

EC number: =2.1.1.34 [H]

Molecular weight: Translated: 25515; Mature: 25515

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSKRYERICEMLARRQPDLTVCMEQVHKPHNVSAVIRTADAVGVHEVHAVWPSSRMRTM
CCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHEECCCHHHHHH
ASAAAGSNSWVQVKTHRTIADAVGHLKGQGMQILATHLSDKAIDFREIDYTRPTCILMGQ
HHHHCCCCCEEEEEECHHHHHHHHHHCCCCHHHHHHHHCCCCCCCEECCCCCCEEEEECC
EKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYEAQRQRQNAGMYQRANSMLPP
CCCCCHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCC
QEQQRLLFEGGYPVLARVARQKGLPYPHVNEQGEVEADAAWWATMQAAR
HHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MNSKRYERICEMLARRQPDLTVCMEQVHKPHNVSAVIRTADAVGVHEVHAVWPSSRMRTM
CCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHEECCCHHHHHH
ASAAAGSNSWVQVKTHRTIADAVGHLKGQGMQILATHLSDKAIDFREIDYTRPTCILMGQ
HHHHCCCCCEEEEEECHHHHHHHHHHCCCCHHHHHHHHCCCCCCCEECCCCCCEEEEECC
EKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYEAQRQRQNAGMYQRANSMLPP
CCCCCHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCC
QEQQRLLFEGGYPVLARVARQKGLPYPHVNEQGEVEADAAWWATMQAAR
HHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]