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The map label for this gene is ligB [H]

Identifier: 238897097

GI number: 238897097

Start: 5090406

End: 5092103

Strand: Reverse

Name: ligB [H]

Synonym: KP1_5350

Alternate gene names: 238897097

Gene position: 5092103-5090406 (Counterclockwise)

Preceding gene: 238897105

Following gene: 238897095

Centisome position: 97.02

GC content: 63.49

Gene sequence:

>1698_bases
GTGATGGAGGAGGGGACTGCAATGCGTAAAGGAGGGTGGTGGCTGGCGTTAGGGATGTTCTCTGCCAGCGCGCTGGCGAC
GTGTCCGGACTGGCCGCCGGCCAGAGGAAGGCAGGAGACATCACGCCTTCATCAGCAAATTGTCGCCTGGAAAGAGGCTT
ACTGGCGGCAGGGCGCCAGCGGAGTGAGCGATGACGTTTACGACCAGCTCACCCTGCGCCTGGCGCAGTGGCGACAATGC
TTTCCGGGCGCGACGCCTGAGGATGACGATCTGCCTCCGCCCACCGGCGATGCGCGTCATCCGGTGGCCCATACCGGCGT
GCGCAAGCTGGCGGATGAGGACAGCGTTGCCCGCTGGATGAAAAATAAATCCGACCTGTGGATACAGCCTAAAGTGGACG
GCGTGGCGGTGACTCTCGTTTATCGCCAGGGGCGTCTGGTTCAGGCCATTAGCCGCGGCGATGGTCTACGCGGTGAGGCA
TGGACCGCCCGAGCGCGGCAGATCCCGGCGCTGGCGAAAGTGATGACCGGTGAACTGGCCGACAGCGTACTGCAGGGGGA
ACTGTTTCTGCGCCGGGACGGCCATGTCCAGCAGCGGGCCGGGGGGATGAATGCGCGGGCGAAAGTCGCCGGGTTGATGA
TGCGAGCCGACGCGGCGGCGGCGCTCAGTCAACTGGACGTTTTTATCTGGGCCTGGCCAGACGGCCCGTCGGATATGCGC
CGTCGTCAGCAGCTGCTGGCGCAGGCGGGGTTTAAGTACAGCGGACAATATACCCATCCGGTGTCGCGCATCGAGCAGGT
GGCGCAGTGGCGCCAGCGCTGGTATCGCTCGCCGCTGCCCTTCGTCAGCGACGGTGTGATTGTGCGCGAAGGACGCGAGC
CGCCGGGACGAGTCTGGTCGCCCGGAAAGGGCGAGTGGCTGGCAGCCTGGAAATATCCTCCGGCATCGCGGGTGATGCAG
GTACGGGCGATCCGTTTCAGTACCGGCCGCAGCGGCAGACTCAACGTGGTGGCCGAGCTTGAGCCCCAGCGGCTGGATGA
TAAACGCGTGCAGCGGGTTAACGTTGGCTCTGTGTCCCGCTGGCAGATGCTGGATATTGGGGTTGGCGATCAGCTGCAGA
TCAGTCTGGCAGGGCAGGGTATTCCTCGGGTCGACGCCGTCGTCTGGCGGACGGCCGAGCGCCATAAACCGACGCCGCCG
CCGGCGAAATTTAATGCGCTAACCTGCTATTTCGCCACCCCTGAGTGCAGCGAACAGTTTCTTTCCAGGCTGATCTGGTT
GTCATCAAAGTCCGCCCTGAATGTCGATGGCGTGGGGGAAAATTTATGGCGCGTCATTCAGCAGCAAAACCCGATGACGC
ATATCTTTTCTTGGCTGGCGCTTACCGTTGAGCAGCTGCAGGCGTTGCCAGGGATTTCCGCCGCCCGCGGGCAGCACCTC
TGGCATCAGTTTGATCTCGTTCGCAAACGACCTTTTATCCGCTGGGTGCTGGCGATGGGGATCCCGGTGCCGCAGGGGGC
GCTGGCGCAGCTCGAAAGCGAGAACTGGCATTTGCTGGCGGCAAAAAGTGAAGCGCAATGGCGCACGCTGCCGGGCGTGG
GGGAGATCAGAGCCCGCCAGCTGGTGGCTTTCCTGCACCATCCGGACGTGGTGGCGCTGGCGCAATGGCTTAGCGGGCAG
CGCATCCCCGGATTTTAA

Upstream 100 bases:

>100_bases
CAGCCGTTGCCGCAAAGAGGCGTAAATCCTGGAAGGCGCTTCGCAAGGCGAAAAAGCTTCCGTCGCGGCTCGCGGCATTC
TGCAGCAGACTCTCCGCCAG

Downstream 100 bases:

>100_bases
GGCGGTTAGTGGGCGTCATGGGTGTAGTGGAAGACCGGTAGCCCCAGTTTCCAGCGTAGCGCCAGCATGCGGGCGGTAAA
GCCGAACAGCAGTGTAGCCA

Product: NAD-dependent DNA ligase LigB

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] B [H]

Number of amino acids: Translated: 565; Mature: 565

Protein sequence:

>565_residues
MMEEGTAMRKGGWWLALGMFSASALATCPDWPPARGRQETSRLHQQIVAWKEAYWRQGASGVSDDVYDQLTLRLAQWRQC
FPGATPEDDDLPPPTGDARHPVAHTGVRKLADEDSVARWMKNKSDLWIQPKVDGVAVTLVYRQGRLVQAISRGDGLRGEA
WTARARQIPALAKVMTGELADSVLQGELFLRRDGHVQQRAGGMNARAKVAGLMMRADAAAALSQLDVFIWAWPDGPSDMR
RRQQLLAQAGFKYSGQYTHPVSRIEQVAQWRQRWYRSPLPFVSDGVIVREGREPPGRVWSPGKGEWLAAWKYPPASRVMQ
VRAIRFSTGRSGRLNVVAELEPQRLDDKRVQRVNVGSVSRWQMLDIGVGDQLQISLAGQGIPRVDAVVWRTAERHKPTPP
PAKFNALTCYFATPECSEQFLSRLIWLSSKSALNVDGVGENLWRVIQQQNPMTHIFSWLALTVEQLQALPGISAARGQHL
WHQFDLVRKRPFIRWVLAMGIPVPQGALAQLESENWHLLAAKSEAQWRTLPGVGEIRARQLVAFLHHPDVVALAQWLSGQ
RIPGF

Sequences:

>Translated_565_residues
MMEEGTAMRKGGWWLALGMFSASALATCPDWPPARGRQETSRLHQQIVAWKEAYWRQGASGVSDDVYDQLTLRLAQWRQC
FPGATPEDDDLPPPTGDARHPVAHTGVRKLADEDSVARWMKNKSDLWIQPKVDGVAVTLVYRQGRLVQAISRGDGLRGEA
WTARARQIPALAKVMTGELADSVLQGELFLRRDGHVQQRAGGMNARAKVAGLMMRADAAAALSQLDVFIWAWPDGPSDMR
RRQQLLAQAGFKYSGQYTHPVSRIEQVAQWRQRWYRSPLPFVSDGVIVREGREPPGRVWSPGKGEWLAAWKYPPASRVMQ
VRAIRFSTGRSGRLNVVAELEPQRLDDKRVQRVNVGSVSRWQMLDIGVGDQLQISLAGQGIPRVDAVVWRTAERHKPTPP
PAKFNALTCYFATPECSEQFLSRLIWLSSKSALNVDGVGENLWRVIQQQNPMTHIFSWLALTVEQLQALPGISAARGQHL
WHQFDLVRKRPFIRWVLAMGIPVPQGALAQLESENWHLLAAKSEAQWRTLPGVGEIRARQLVAFLHHPDVVALAQWLSGQ
RIPGF
>Mature_565_residues
MMEEGTAMRKGGWWLALGMFSASALATCPDWPPARGRQETSRLHQQIVAWKEAYWRQGASGVSDDVYDQLTLRLAQWRQC
FPGATPEDDDLPPPTGDARHPVAHTGVRKLADEDSVARWMKNKSDLWIQPKVDGVAVTLVYRQGRLVQAISRGDGLRGEA
WTARARQIPALAKVMTGELADSVLQGELFLRRDGHVQQRAGGMNARAKVAGLMMRADAAAALSQLDVFIWAWPDGPSDMR
RRQQLLAQAGFKYSGQYTHPVSRIEQVAQWRQRWYRSPLPFVSDGVIVREGREPPGRVWSPGKGEWLAAWKYPPASRVMQ
VRAIRFSTGRSGRLNVVAELEPQRLDDKRVQRVNVGSVSRWQMLDIGVGDQLQISLAGQGIPRVDAVVWRTAERHKPTPP
PAKFNALTCYFATPECSEQFLSRLIWLSSKSALNVDGVGENLWRVIQQQNPMTHIFSWLALTVEQLQALPGISAARGQHL
WHQFDLVRKRPFIRWVLAMGIPVPQGALAQLESENWHLLAAKSEAQWRTLPGVGEIRARQLVAFLHHPDVVALAQWLSGQ
RIPGF

Specific function: Catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction [H]

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD-dependent DNA ligase family. LigB subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082305, Length=551, Percent_Identity=54.0834845735027, Blast_Score=632, Evalue=0.0,
Organism=Escherichia coli, GI1788750, Length=565, Percent_Identity=25.1327433628319, Blast_Score=107, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020923
- InterPro:   IPR004150
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994 [H]

Pfam domain/function: PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB [H]

EC number: =6.5.1.2 [H]

Molecular weight: Translated: 63426; Mature: 63426

Theoretical pI: Translated: 10.58; Mature: 10.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMEEGTAMRKGGWWLALGMFSASALATCPDWPPARGRQETSRLHQQIVAWKEAYWRQGAS
CCCCCCCCCCCCEEEEEEHHCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
GVSDDVYDQLTLRLAQWRQCFPGATPEDDDLPPPTGDARHPVAHTGVRKLADEDSVARWM
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHH
KNKSDLWIQPKVDGVAVTLVYRQGRLVQAISRGDGLRGEAWTARARQIPALAKVMTGELA
CCCCCEEEECCCCCEEEEEEEECCHHHHHHHCCCCCCCCHHHHHHHHCHHHHHHHHHHHH
DSVLQGELFLRRDGHVQQRAGGMNARAKVAGLMMRADAAAALSQLDVFIWAWPDGPSDMR
HHHHHCCEEEECCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCEEEEEECCCCHHHHH
RRQQLLAQAGFKYSGQYTHPVSRIEQVAQWRQRWYRSPLPFVSDGVIVREGREPPGRVWS
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCC
PGKGEWLAAWKYPPASRVMQVRAIRFSTGRSGRLNVVAELEPQRLDDKRVQRVNVGSVSR
CCCCCEEEEECCCCHHHHHHHHEEEECCCCCCCEEEEEECCCCCCCHHHHHEECCCCCCC
WQMLDIGVGDQLQISLAGQGIPRVDAVVWRTAERHKPTPPPAKFNALTCYFATPECSEQF
EEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCHHHHHH
LSRLIWLSSKSALNVDGVGENLWRVIQQQNPMTHIFSWLALTVEQLQALPGISAARGQHL
HHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
WHQFDLVRKRPFIRWVLAMGIPVPQGALAQLESENWHLLAAKSEAQWRTLPGVGEIRARQ
HHHHHHHHHCHHHHHHHHHCCCCCCCHHHHHCCCCEEEEEECCCCCCCCCCCCHHHHHHH
LVAFLHHPDVVALAQWLSGQRIPGF
HHHHHCCCCHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MMEEGTAMRKGGWWLALGMFSASALATCPDWPPARGRQETSRLHQQIVAWKEAYWRQGAS
CCCCCCCCCCCCEEEEEEHHCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
GVSDDVYDQLTLRLAQWRQCFPGATPEDDDLPPPTGDARHPVAHTGVRKLADEDSVARWM
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHH
KNKSDLWIQPKVDGVAVTLVYRQGRLVQAISRGDGLRGEAWTARARQIPALAKVMTGELA
CCCCCEEEECCCCCEEEEEEEECCHHHHHHHCCCCCCCCHHHHHHHHCHHHHHHHHHHHH
DSVLQGELFLRRDGHVQQRAGGMNARAKVAGLMMRADAAAALSQLDVFIWAWPDGPSDMR
HHHHHCCEEEECCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCEEEEEECCCCHHHHH
RRQQLLAQAGFKYSGQYTHPVSRIEQVAQWRQRWYRSPLPFVSDGVIVREGREPPGRVWS
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCC
PGKGEWLAAWKYPPASRVMQVRAIRFSTGRSGRLNVVAELEPQRLDDKRVQRVNVGSVSR
CCCCCEEEEECCCCHHHHHHHHEEEECCCCCCCEEEEEECCCCCCCHHHHHEECCCCCCC
WQMLDIGVGDQLQISLAGQGIPRVDAVVWRTAERHKPTPPPAKFNALTCYFATPECSEQF
EEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCHHHHHH
LSRLIWLSSKSALNVDGVGENLWRVIQQQNPMTHIFSWLALTVEQLQALPGISAARGQHL
HHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
WHQFDLVRKRPFIRWVLAMGIPVPQGALAQLESENWHLLAAKSEAQWRTLPGVGEIRARQ
HHHHHHHHHCHHHHHHHHHCCCCCCCHHHHHCCCCEEEEEECCCCCCCCCCCCHHHHHHH
LVAFLHHPDVVALAQWLSGQRIPGF
HHHHHCCCCHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA