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The map label for this gene is prpB [C]

Identifier: 238897093

GI number: 238897093

Start: 5086394

End: 5087140

Strand: Direct

Name: prpB [C]

Synonym: KP1_5346

Alternate gene names: 238897093

Gene position: 5086394-5087140 (Clockwise)

Preceding gene: 238897092

Following gene: 238897094

Centisome position: 96.91

GC content: 51.81

Gene sequence:

>747_bases
ATGGACTTTAATGCGCTTCACCAGCAACACCAACCTTTGTTGCTTGCCAATGTCTGGGATGCCAGCAGCGCTCAGGCTGC
TCAGCAGGCTGGTTATCAGGCGCTGGGATCCTCCAGCGCTGCTATCGCCGCAATGTTAGGGTATGAAGATGGCGAAGAGA
TGAGTTTTGACGAGCTGTTTTATGTCGTCTCACGCATCAAAACCGTTAGCGAACTGCCGTTAAGCGTCGATTTAGAGGCG
GGCTACGGCGCGACAACCAGCCACATCATTGACAATATCCGGCGTCTGGCGCATCTCGGCGTGAGTGGAATAAATCTCGA
GGATAGTCATGTGGTGGATGGTACGCGTCGTCTTGACGATGCGGAGCGCTTTGCCGTCAAACTGCAGGAAATAACCCGCG
CCTGTCCCGGTCTGTTTGTTAATGTCCGCACCGATACCTTTCTTCTCAACGTACAAGATGCGCTGGTTCAGACGCTCTAT
CGCGGTCAACTCTACGCCAAACACGGCGCATGCGGTTTTTTTGTGCCTTGCGTCACCCGCGCGGAGGATATTATCGCCAT
CGTTCACCACGTGCGTTTACCCTTAAACGTAATGTGTATGCCTGAGCTGGCTGACTTCAGCACCTTATCGACACTGGGCG
TAAAACGAATTTCGATGGGCAATTTTATTTATGCTGCGACGCAGGCCAGACTGAAAGACTTATTATGTCAGGTGCAAACC
CAACACTCTTTCTCAGGTGTTTTTTGA

Upstream 100 bases:

>100_bases
TGATAAGAATCTACTTATAAAAAATAGTCGTCCAGTTTTCGGTTGTGGCAAATAAGCACAACGCGGCACAGTCAGGAAAA
CACTACCGCGAGAGAAAAGA

Downstream 100 bases:

>100_bases
CCATGAAAATTACCGACAGCATGCAGTGCGATATCTGGTATCAGGCGCTGCTTGAACGCGCGGTGGAATTCACCGGCGTT
TTTTTCGTCGGCGTGAAAAC

Product: hypothetical protein

Products: Pyruvate; Succinate [C]

Alternate protein names: NA

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MDFNALHQQHQPLLLANVWDASSAQAAQQAGYQALGSSSAAIAAMLGYEDGEEMSFDELFYVVSRIKTVSELPLSVDLEA
GYGATTSHIIDNIRRLAHLGVSGINLEDSHVVDGTRRLDDAERFAVKLQEITRACPGLFVNVRTDTFLLNVQDALVQTLY
RGQLYAKHGACGFFVPCVTRAEDIIAIVHHVRLPLNVMCMPELADFSTLSTLGVKRISMGNFIYAATQARLKDLLCQVQT
QHSFSGVF

Sequences:

>Translated_248_residues
MDFNALHQQHQPLLLANVWDASSAQAAQQAGYQALGSSSAAIAAMLGYEDGEEMSFDELFYVVSRIKTVSELPLSVDLEA
GYGATTSHIIDNIRRLAHLGVSGINLEDSHVVDGTRRLDDAERFAVKLQEITRACPGLFVNVRTDTFLLNVQDALVQTLY
RGQLYAKHGACGFFVPCVTRAEDIIAIVHHVRLPLNVMCMPELADFSTLSTLGVKRISMGNFIYAATQARLKDLLCQVQT
QHSFSGVF
>Mature_248_residues
MDFNALHQQHQPLLLANVWDASSAQAAQQAGYQALGSSSAAIAAMLGYEDGEEMSFDELFYVVSRIKTVSELPLSVDLEA
GYGATTSHIIDNIRRLAHLGVSGINLEDSHVVDGTRRLDDAERFAVKLQEITRACPGLFVNVRTDTFLLNVQDALVQTLY
RGQLYAKHGACGFFVPCVTRAEDIIAIVHHVRLPLNVMCMPELADFSTLSTLGVKRISMGNFIYAATQARLKDLLCQVQT
QHSFSGVF

Specific function: Catalyzes The Formation Of Pyruvate And Succinate From 2-Methylisocitrate (By Similarity). [C]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786525, Length=189, Percent_Identity=26.984126984127, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR015813 [H]

Pfam domain/function: NA

EC number: 4.1.3.30 [C]

Molecular weight: Translated: 27233; Mature: 27233

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFNALHQQHQPLLLANVWDASSAQAAQQAGYQALGSSSAAIAAMLGYEDGEEMSFDELF
CCCHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCHHHHH
YVVSRIKTVSELPLSVDLEAGYGATTSHIIDNIRRLAHLGVSGINLEDSHVVDGTRRLDD
HHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCH
AERFAVKLQEITRACPGLFVNVRTDTFLLNVQDALVQTLYRGQLYAKHGACGFFVPCVTR
HHHHHHHHHHHHHHCCCEEEEEECCEEEEEHHHHHHHHHHHCCCEECCCCCEEHHHHHHH
AEDIIAIVHHVRLPLNVMCMPELADFSTLSTLGVKRISMGNFIYAATQARLKDLLCQVQT
HHHHHHHHHHHCCCCEEEECCCHHCHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHH
QHSFSGVF
CCCCCCCC
>Mature Secondary Structure
MDFNALHQQHQPLLLANVWDASSAQAAQQAGYQALGSSSAAIAAMLGYEDGEEMSFDELF
CCCHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCHHHHH
YVVSRIKTVSELPLSVDLEAGYGATTSHIIDNIRRLAHLGVSGINLEDSHVVDGTRRLDD
HHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCH
AERFAVKLQEITRACPGLFVNVRTDTFLLNVQDALVQTLYRGQLYAKHGACGFFVPCVTR
HHHHHHHHHHHHHHCCCEEEEEECCEEEEEHHHHHHHHHHHCCCEECCCCCEEHHHHHHH
AEDIIAIVHHVRLPLNVMCMPELADFSTLSTLGVKRISMGNFIYAATQARLKDLLCQVQT
HHHHHHHHHHHCCCCEEEECCCHHCHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHH
QHSFSGVF
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: methylisocitrate [C]

Specific reaction: methylisocitrate <==> Pyruvate + Succinate [C]

General reaction: Elimination [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]