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The map label for this gene is hydN [H]

Identifier: 238897038

GI number: 238897038

Start: 5032659

End: 5033144

Strand: Reverse

Name: hydN [H]

Synonym: KP1_5285

Alternate gene names: 238897038

Gene position: 5033144-5032659 (Counterclockwise)

Preceding gene: 238897039

Following gene: 238897035

Centisome position: 95.9

GC content: 65.23

Gene sequence:

>486_bases
ATGAACCAGTTTCTTATTGCCAATGCCCAGCGGTGCATCGGCTGTCGTACCTGCGAAGTGGCCTGCGCGGTGGCGCACCA
GCAGGCGCAGGATGTCGCCGCCCTGTCGACGAACCATTTCGCGCCGCGTATCCGGGTGGTGAAAAGCGGTGACATATCGA
CGGCGATGGCCTGTCGGCAGTGCGAGGATGCGCCCTGCGCCAGCGTTTGCCCACAGGGGGCGATTCAGCGCGACAATGAC
GTCTGGTGGGTCGATCAGCGGCGCTGCATCGGCTGCAAAAGCTGCATGGTGGCCTGCCCGTACGGCGCGATGACCGTTAC
GGTAATGAACCAGCAGGCGCAGGCGTTGAAATGCGATCTATGCCATCACCGCGCCGAAGGACCGGCCTGCGTGGCCGCCT
GCCCGACGCAGGCGCTGCGGGTGATGGTTCCCGCGGAGCTGGAAGCGCTATGCGCGCAAAAACGCCAGCGGCTGGCGCTG
GCGTGA

Upstream 100 bases:

>100_bases
GCCGGCCATCTGACAAAAAACAGCGCTGATTCCATTCAGGATCAAAGCGCGGGCAGGGACTATTCTGGCACCCTGCGGTT
TTATCCCCAGGGAGCAGAGG

Downstream 100 bases:

>100_bases
ATGGGGTCGCTGACGAACGTTACCCGCCCGCGGCGTCAGAGATTATGCTTCCTGTTCGCGCCAGGCAAATTCAACCTCTT
CGGCGAGGATTTTCACTCCG

Product: electron transport protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 161; Mature: 161

Protein sequence:

>161_residues
MNQFLIANAQRCIGCRTCEVACAVAHQQAQDVAALSTNHFAPRIRVVKSGDISTAMACRQCEDAPCASVCPQGAIQRDND
VWWVDQRRCIGCKSCMVACPYGAMTVTVMNQQAQALKCDLCHHRAEGPACVAACPTQALRVMVPAELEALCAQKRQRLAL
A

Sequences:

>Translated_161_residues
MNQFLIANAQRCIGCRTCEVACAVAHQQAQDVAALSTNHFAPRIRVVKSGDISTAMACRQCEDAPCASVCPQGAIQRDND
VWWVDQRRCIGCKSCMVACPYGAMTVTVMNQQAQALKCDLCHHRAEGPACVAACPTQALRVMVPAELEALCAQKRQRLAL
A
>Mature_161_residues
MNQFLIANAQRCIGCRTCEVACAVAHQQAQDVAALSTNHFAPRIRVVKSGDISTAMACRQCEDAPCASVCPQGAIQRDND
VWWVDQRRCIGCKSCMVACPYGAMTVTVMNQQAQALKCDLCHHRAEGPACVAACPTQALRVMVPAELEALCAQKRQRLAL
A

Specific function: Electron transport from formate to hydrogen [H]

COG id: COG1142

COG function: function code C; Fe-S-cluster-containing hydrogenase components 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1789067, Length=173, Percent_Identity=58.3815028901734, Blast_Score=195, Evalue=1e-51,
Organism=Escherichia coli, GI2367245, Length=142, Percent_Identity=62.6760563380282, Blast_Score=182, Evalue=8e-48,
Organism=Escherichia coli, GI1788811, Length=164, Percent_Identity=46.9512195121951, Blast_Score=153, Evalue=6e-39,
Organism=Escherichia coli, GI87082179, Length=149, Percent_Identity=46.9798657718121, Blast_Score=141, Evalue=2e-35,
Organism=Escherichia coli, GI87082180, Length=160, Percent_Identity=43.75, Blast_Score=129, Evalue=9e-32,
Organism=Escherichia coli, GI1789079, Length=188, Percent_Identity=34.5744680851064, Blast_Score=107, Evalue=5e-25,
Organism=Escherichia coli, GI87082114, Length=194, Percent_Identity=35.0515463917526, Blast_Score=103, Evalue=6e-24,
Organism=Escherichia coli, GI1787872, Length=173, Percent_Identity=34.1040462427746, Blast_Score=84, Evalue=3e-18,
Organism=Escherichia coli, GI1787122, Length=161, Percent_Identity=34.7826086956522, Blast_Score=84, Evalue=5e-18,
Organism=Escherichia coli, GI2367345, Length=170, Percent_Identity=31.7647058823529, Blast_Score=74, Evalue=4e-15,
Organism=Escherichia coli, GI226510944, Length=145, Percent_Identity=36.551724137931, Blast_Score=74, Evalue=5e-15,
Organism=Escherichia coli, GI1787963, Length=156, Percent_Identity=33.974358974359, Blast_Score=74, Evalue=6e-15,
Organism=Escherichia coli, GI1789370, Length=184, Percent_Identity=27.7173913043478, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1787749, Length=174, Percent_Identity=28.735632183908, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: NA

Molecular weight: Translated: 17407; Mature: 17407

Theoretical pI: Translated: 7.98; Mature: 7.98

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

10.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
14.3 %Cys+Met (Translated Protein)
10.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
14.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQFLIANAQRCIGCRTCEVACAVAHQQAQDVAALSTNHFAPRIRVVKSGDISTAMACRQ
CCCEEECCCHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHH
CEDAPCASVCPQGAIQRDNDVWWVDQRRCIGCKSCMVACPYGAMTVTVMNQQAQALKCDL
CCCCCCHHHCCCCCCCCCCCEEEECCCCCCCCCHHHEECCCCCEEEEEECCCHHHHHHHH
CHHRAEGPACVAACPTQALRVMVPAELEALCAQKRQRLALA
HHCCCCCCEEEEECCHHHHEEECCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNQFLIANAQRCIGCRTCEVACAVAHQQAQDVAALSTNHFAPRIRVVKSGDISTAMACRQ
CCCEEECCCHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHH
CEDAPCASVCPQGAIQRDNDVWWVDQRRCIGCKSCMVACPYGAMTVTVMNQQAQALKCDL
CCCCCCHHHCCCCCCCCCCCEEEECCCCCCCCCHHHEECCCCCEEEEEECCCHHHHHHHH
CHHRAEGPACVAACPTQALRVMVPAELEALCAQKRQRLALA
HHCCCCCCEEEEECCHHHHEEECCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]