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The map label for this gene is pcaK [H]

Identifier: 238897017

GI number: 238897017

Start: 5008948

End: 5010303

Strand: Direct

Name: pcaK [H]

Synonym: KP1_5263

Alternate gene names: 238897017

Gene position: 5008948-5010303 (Clockwise)

Preceding gene: 238897015

Following gene: 238897018

Centisome position: 95.44

GC content: 61.58

Gene sequence:

>1356_bases
ATGACTAAGACAACCATCGACATTCAGGCCTTTATCAACGAGCACCCCTTCACCCGCTACCAGTGGATGATCCTTGCGCT
CTGCTTTATCACCGTCGCGATGGACGGGTTCGATACCGCAATTATCGGGTTCATCGCCTCCGACCTGGTGCAAGAGTGGG
GCGTGGAAAAATCGGCCCTCGGCCCGGTGATGAGCGCCGCGCTGGTGGGCCTGGCGGTCGGCGCCTTAACCGCAGGGCCG
CTGGCTGACCGCATCGGCCGCAAAAAAGTGCTGATCATGTCGATCGTGGTGTTCGGCGGATTCAGCCTGCTCACCGCCTT
CGCCACCAGCCTTAATCAGCTGACGCTGCTGCGCTTCCTGACCGGTCTGGGCCTTGGCGCGGCGATGCCGAATGCCGCGA
CGCTAATGAGTGAATATGCGCCGGAGCGCCGTCGGGCGCTGCTGGTGAACCTGATGTTTGTCGGCTTTCCGATGGGATCT
TCGCTGGGCGGCTTTTTATCCGCCTGGATGATCCCGCACTATGGCTGGCAGAGCGTGCTGGTGCTGGGCGGCGTGATGCC
CCTCCTGCTGGCGGTGGCATTAATATTCCTGCTGCCAGAGTCCGTCCGCTATCTGGTGGTGAAGCAGCACCCGGCGCAGC
GCATTGCTGCTATCCTGCGCCGTATCGCGCCGCTGCCGAAGGCAGTGGAATTTGTACTACGCGAAGCAGGCCAGGTGAAA
GAGAAATCCGCGATCGGCGTCATTTTCTCCCCTCGCTACGCGGTGGGTACCGTGATGCTGTGCCTGACCTACTTTATGGG
TCTGCTGATTTTCTATCTGCTGACCAGCTGGCTGCCGCTGCTCATCCGTGAAACCGGCGCCTCGATGAGTCAGGCCTCAA
TTATCACCGCCCTGTTCCCGCTCGGCGGCGGGATTGGCGTGCTGATCCTCGGCGCCCTGATGGACAAGATCAACCCCAAT
AAAGTGGTGGCCGTCGGCTGGCTGCTGACCGGCGTTTTTGTCTGCCTGGTGGGTTTCTCCACCAGCAGCCTGGCGCTAAT
GGGAGTGATGGTCTTTATCGCCGGCAGCATTATGAACGGCGCGCAGTCGTCGATGCCGGCGCTGGCGGCTGGCTTCTATC
CGACGCAGGGGCGCGCCACCGGCGTCGCCTGGATGCTGGGTATCGGCCGCTTCGGCGGCATCCTCGGCGCGTTCAGCGGC
GCCTTCCTGATGCAGGCTCAGCTCTCCTTTGAGACCATTTTCACCCTGCTGGCCATCCCGGCGTTCCTGTCAGCCATTGC
GCTGCTGATCAAGTACCGGGTCAGTAAATCCGTGCCGGCGACGACAGATGAGGCGAGAAGCCTGCAGAAAGCCTAA

Upstream 100 bases:

>100_bases
ACGGCGACATGTTCGATTATCGCACAAATCAAACTCAACGACGCGAAGGCGACGATAATAATAAACCTCTGTCCCTCGCT
CAGTCTGCCAGGAAAGCCGT

Downstream 100 bases:

>100_bases
CCCTATAAGCCGGCGCCCCGCGCCGGCTTTGTCGCTTTTTTCTTATCCGTTTACAAAAATTTTCGAAGCCACCTCATCGC
GTTAATAATCATTCTTTTAG

Product: putative permease of the major facilitator superfamily

Products: Proton [Cytoplasm]; 3-(3-hydroxyphenyl)propionate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 451; Mature: 450

Protein sequence:

>451_residues
MTKTTIDIQAFINEHPFTRYQWMILALCFITVAMDGFDTAIIGFIASDLVQEWGVEKSALGPVMSAALVGLAVGALTAGP
LADRIGRKKVLIMSIVVFGGFSLLTAFATSLNQLTLLRFLTGLGLGAAMPNAATLMSEYAPERRRALLVNLMFVGFPMGS
SLGGFLSAWMIPHYGWQSVLVLGGVMPLLLAVALIFLLPESVRYLVVKQHPAQRIAAILRRIAPLPKAVEFVLREAGQVK
EKSAIGVIFSPRYAVGTVMLCLTYFMGLLIFYLLTSWLPLLIRETGASMSQASIITALFPLGGGIGVLILGALMDKINPN
KVVAVGWLLTGVFVCLVGFSTSSLALMGVMVFIAGSIMNGAQSSMPALAAGFYPTQGRATGVAWMLGIGRFGGILGAFSG
AFLMQAQLSFETIFTLLAIPAFLSAIALLIKYRVSKSVPATTDEARSLQKA

Sequences:

>Translated_451_residues
MTKTTIDIQAFINEHPFTRYQWMILALCFITVAMDGFDTAIIGFIASDLVQEWGVEKSALGPVMSAALVGLAVGALTAGP
LADRIGRKKVLIMSIVVFGGFSLLTAFATSLNQLTLLRFLTGLGLGAAMPNAATLMSEYAPERRRALLVNLMFVGFPMGS
SLGGFLSAWMIPHYGWQSVLVLGGVMPLLLAVALIFLLPESVRYLVVKQHPAQRIAAILRRIAPLPKAVEFVLREAGQVK
EKSAIGVIFSPRYAVGTVMLCLTYFMGLLIFYLLTSWLPLLIRETGASMSQASIITALFPLGGGIGVLILGALMDKINPN
KVVAVGWLLTGVFVCLVGFSTSSLALMGVMVFIAGSIMNGAQSSMPALAAGFYPTQGRATGVAWMLGIGRFGGILGAFSG
AFLMQAQLSFETIFTLLAIPAFLSAIALLIKYRVSKSVPATTDEARSLQKA
>Mature_450_residues
TKTTIDIQAFINEHPFTRYQWMILALCFITVAMDGFDTAIIGFIASDLVQEWGVEKSALGPVMSAALVGLAVGALTAGPL
ADRIGRKKVLIMSIVVFGGFSLLTAFATSLNQLTLLRFLTGLGLGAAMPNAATLMSEYAPERRRALLVNLMFVGFPMGSS
LGGFLSAWMIPHYGWQSVLVLGGVMPLLLAVALIFLLPESVRYLVVKQHPAQRIAAILRRIAPLPKAVEFVLREAGQVKE
KSAIGVIFSPRYAVGTVMLCLTYFMGLLIFYLLTSWLPLLIRETGASMSQASIITALFPLGGGIGVLILGALMDKINPNK
VVAVGWLLTGVFVCLVGFSTSSLALMGVMVFIAGSIMNGAQSSMPALAAGFYPTQGRATGVAWMLGIGRFGGILGAFSGA
FLMQAQLSFETIFTLLAIPAFLSAIALLIKYRVSKSVPATTDEARSLQKA

Specific function: Transporter for 4-hydroxybenzoate. Also required for chemotaxis to aromatic acids [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Homo sapiens, GI24308167, Length=440, Percent_Identity=25.9090909090909, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI7662270, Length=375, Percent_Identity=22.9333333333333, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI213021148, Length=461, Percent_Identity=23.4273318872017, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI216548223, Length=340, Percent_Identity=23.5294117647059, Blast_Score=79, Evalue=8e-15,
Organism=Homo sapiens, GI262399383, Length=264, Percent_Identity=25.7575757575758, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI73695465, Length=212, Percent_Identity=25.4716981132075, Blast_Score=74, Evalue=4e-13,
Organism=Homo sapiens, GI154350196, Length=424, Percent_Identity=23.5849056603774, Blast_Score=72, Evalue=9e-13,
Organism=Escherichia coli, GI87081723, Length=394, Percent_Identity=37.0558375634518, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI87082231, Length=455, Percent_Identity=22.8571428571429, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI87082159, Length=391, Percent_Identity=25.0639386189258, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI87082404, Length=367, Percent_Identity=22.3433242506812, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1788074, Length=390, Percent_Identity=24.3589743589744, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1786229, Length=175, Percent_Identity=27.4285714285714, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1789152, Length=419, Percent_Identity=22.4343675417661, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1789312, Length=236, Percent_Identity=30.9322033898305, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1789207, Length=203, Percent_Identity=30.5418719211823, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1788068, Length=439, Percent_Identity=22.3234624145786, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI48994889, Length=172, Percent_Identity=27.3255813953488, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI87082320, Length=310, Percent_Identity=22.258064516129, Blast_Score=62, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI32564663, Length=460, Percent_Identity=22.1739130434783, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24640198, Length=452, Percent_Identity=23.8938053097345, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24640196, Length=452, Percent_Identity=23.8938053097345, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24640200, Length=452, Percent_Identity=23.8938053097345, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI19922874, Length=419, Percent_Identity=24.582338902148, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24646916, Length=366, Percent_Identity=25.6830601092896, Blast_Score=69, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004746
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 48072; Mature: 47941

Theoretical pI: Translated: 10.24; Mature: 10.24

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKTTIDIQAFINEHPFTRYQWMILALCFITVAMDGFDTAIIGFIASDLVQEWGVEKSAL
CCCCEEHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHH
GPVMSAALVGLAVGALTAGPLADRIGRKKVLIMSIVVFGGFSLLTAFATSLNQLTLLRFL
HHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TGLGLGAAMPNAATLMSEYAPERRRALLVNLMFVGFPMGSSLGGFLSAWMIPHYGWQSVL
HHCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHH
VLGGVMPLLLAVALIFLLPESVRYLVVKQHPAQRIAAILRRIAPLPKAVEFVLREAGQVK
HHHHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCHH
EKSAIGVIFSPRYAVGTVMLCLTYFMGLLIFYLLTSWLPLLIRETGASMSQASIITALFP
HHCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
LGGGIGVLILGALMDKINPNKVVAVGWLLTGVFVCLVGFSTSSLALMGVMVFIAGSIMNG
CCCCHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
AQSSMPALAAGFYPTQGRATGVAWMLGIGRFGGILGAFSGAFLMQAQLSFETIFTLLAIP
CHHCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFLSAIALLIKYRVSKSVPATTDEARSLQKA
HHHHHHHHHHHHHHCCCCCCCCHHHHHHCCC
>Mature Secondary Structure 
TKTTIDIQAFINEHPFTRYQWMILALCFITVAMDGFDTAIIGFIASDLVQEWGVEKSAL
CCCEEHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHH
GPVMSAALVGLAVGALTAGPLADRIGRKKVLIMSIVVFGGFSLLTAFATSLNQLTLLRFL
HHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TGLGLGAAMPNAATLMSEYAPERRRALLVNLMFVGFPMGSSLGGFLSAWMIPHYGWQSVL
HHCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHH
VLGGVMPLLLAVALIFLLPESVRYLVVKQHPAQRIAAILRRIAPLPKAVEFVLREAGQVK
HHHHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCHH
EKSAIGVIFSPRYAVGTVMLCLTYFMGLLIFYLLTSWLPLLIRETGASMSQASIITALFP
HHCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
LGGGIGVLILGALMDKINPNKVVAVGWLLTGVFVCLVGFSTSSLALMGVMVFIAGSIMNG
CCCCHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
AQSSMPALAAGFYPTQGRATGVAWMLGIGRFGGILGAFSGAFLMQAQLSFETIFTLLAIP
CHHCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFLSAIALLIKYRVSKSVPATTDEARSLQKA
HHHHHHHHHHHHHHCCCCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 3-(3-hydroxyphenyl)propionate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 3-(3-hydroxyphenyl)propionate [Periplasm] = Proton [Cytoplasm] + 3-(3-hydroxyphenyl)propionate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7961399 [H]