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The map label for this gene is tkrA [H]

Identifier: 238897015

GI number: 238897015

Start: 5006717

End: 5007688

Strand: Direct

Name: tkrA [H]

Synonym: KP1_5261

Alternate gene names: 238897015

Gene position: 5006717-5007688 (Clockwise)

Preceding gene: 238897014

Following gene: 238897017

Centisome position: 95.39

GC content: 60.91

Gene sequence:

>972_bases
ATGAAGCCGTCTGTCATTCTCTATAAAACGCTTCCCGACGACCTGCTACAGCGTCTGGAGGAGCACTTTTCCGTCACTCA
GGTGAAAAACCTGCGGCCGGAGACCGTTTCGCAGCACGCTGAGGCCTTCGCCCAGGCTGAAGGCCTGCTGGGCTCCAGCG
AAAAGGTTGACGCCGCGCTGCTGGAGAAGATGCCGAAGCTGCGTGCGACCTCCACCGTTTCCGTTGGCTATGACAACTTC
GACGTCGAGGCGCTGAACGCCCGCCGGGTGCTGCTGATGCATACCCCGACCGTGCTGACCGAGACCGTCGCCGATACGGT
CATGGCGCTGGTGCTGAGTACCGCGCGTCGGGTAGTGGAAGTGGCGGAGCGCGTCAAAGCGGGTGAATGGACGAAGAGCA
TTGGTCCGGACTGGTTCGGCACGGATGTTCACCATAAAACCTTAGGCATCGTCGGCATGGGCCGCATCGGTATGGCGCTG
GCGCAGCGCGCCCACTTCGGGTTCGGCATGCCGATCCTGTATAACGCGCGTCGCCAGCATCCGCAGGCGGAAGAACGCTT
CAATGCCCGCTATTGCGATCTCGATACCCTGCTGCAGGAGGCTGACTTTGTCTGCCTGATCCTGCCCCTGAGCGAAGAGA
CTCACCATCTGTTCGGTCAGGCGCAGTTTGCCAAAATGAAATCTTCCGCCATCTTCATTAACGCCGGGCGTGGCCCGGTA
GTGGACGAGCAGGCGCTGATCGCCGCCCTGCAGAACGGTGAGATCCACGCTGCCGGTCTCGATGTGTTCGAACACGAACC
GCTGGCCAAGGACTCACCGCTGCTGAGCCTGCCGAACGTCGTCGCGCTGCCGCATATCGGCTCCGCCACCCATGAGACGC
GCTACAACATGGCGGCCTGTGCGGTGGACAACCTGATCGACGCCCTGAACGGCAACGTAGAGAAAAACTGCGTCAATCCG
CAGGTAAAATAA

Upstream 100 bases:

>100_bases
CGCCTCAGTATGGCTGACTTTGATTGTTAAGCCTGCTAATAATCAAAACCTTCCACTTGGCGCTCATCACGCCTGAACGA
CTCATACTACGGAGATTCGC

Downstream 100 bases:

>100_bases
GCCATAAAAAAACCCGGGTAACGGCGGCAACCGCCCTCCCCGGGTTCTTTTTGCATCCTGTCCATCATCCTGCCCGTTCG
CCGCAGCCAACGTCAGCCAG

Product: 2-hydroxyacid dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MKPSVILYKTLPDDLLQRLEEHFSVTQVKNLRPETVSQHAEAFAQAEGLLGSSEKVDAALLEKMPKLRATSTVSVGYDNF
DVEALNARRVLLMHTPTVLTETVADTVMALVLSTARRVVEVAERVKAGEWTKSIGPDWFGTDVHHKTLGIVGMGRIGMAL
AQRAHFGFGMPILYNARRQHPQAEERFNARYCDLDTLLQEADFVCLILPLSEETHHLFGQAQFAKMKSSAIFINAGRGPV
VDEQALIAALQNGEIHAAGLDVFEHEPLAKDSPLLSLPNVVALPHIGSATHETRYNMAACAVDNLIDALNGNVEKNCVNP
QVK

Sequences:

>Translated_323_residues
MKPSVILYKTLPDDLLQRLEEHFSVTQVKNLRPETVSQHAEAFAQAEGLLGSSEKVDAALLEKMPKLRATSTVSVGYDNF
DVEALNARRVLLMHTPTVLTETVADTVMALVLSTARRVVEVAERVKAGEWTKSIGPDWFGTDVHHKTLGIVGMGRIGMAL
AQRAHFGFGMPILYNARRQHPQAEERFNARYCDLDTLLQEADFVCLILPLSEETHHLFGQAQFAKMKSSAIFINAGRGPV
VDEQALIAALQNGEIHAAGLDVFEHEPLAKDSPLLSLPNVVALPHIGSATHETRYNMAACAVDNLIDALNGNVEKNCVNP
QVK
>Mature_323_residues
MKPSVILYKTLPDDLLQRLEEHFSVTQVKNLRPETVSQHAEAFAQAEGLLGSSEKVDAALLEKMPKLRATSTVSVGYDNF
DVEALNARRVLLMHTPTVLTETVADTVMALVLSTARRVVEVAERVKAGEWTKSIGPDWFGTDVHHKTLGIVGMGRIGMAL
AQRAHFGFGMPILYNARRQHPQAEERFNARYCDLDTLLQEADFVCLILPLSEETHHLFGQAQFAKMKSSAIFINAGRGPV
VDEQALIAALQNGEIHAAGLDVFEHEPLAKDSPLLSLPNVVALPHIGSATHETRYNMAACAVDNLIDALNGNVEKNCVNP
QVK

Specific function: Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively [H]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrB subfamily [H]

Homologues:

Organism=Homo sapiens, GI6912396, Length=291, Percent_Identity=40.2061855670103, Blast_Score=191, Evalue=8e-49,
Organism=Homo sapiens, GI23308577, Length=314, Percent_Identity=30.5732484076433, Blast_Score=145, Evalue=6e-35,
Organism=Homo sapiens, GI145580578, Length=270, Percent_Identity=31.8518518518519, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI4557499, Length=270, Percent_Identity=31.8518518518519, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI145580575, Length=272, Percent_Identity=31.6176470588235, Blast_Score=128, Evalue=6e-30,
Organism=Homo sapiens, GI61743967, Length=268, Percent_Identity=30.5970149253731, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI4557497, Length=268, Percent_Identity=30.5970149253731, Blast_Score=123, Evalue=2e-28,
Organism=Escherichia coli, GI87082289, Length=322, Percent_Identity=86.3354037267081, Blast_Score=585, Evalue=1e-168,
Organism=Escherichia coli, GI1787645, Length=210, Percent_Identity=30.952380952381, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1789279, Length=263, Percent_Identity=30.4182509505703, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI87081824, Length=254, Percent_Identity=27.5590551181102, Blast_Score=79, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17532191, Length=304, Percent_Identity=30.9210526315789, Blast_Score=143, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI25147481, Length=287, Percent_Identity=28.9198606271777, Blast_Score=105, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6324055, Length=241, Percent_Identity=37.7593360995851, Blast_Score=157, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6320925, Length=261, Percent_Identity=32.183908045977, Blast_Score=125, Evalue=9e-30,
Organism=Saccharomyces cerevisiae, GI6322116, Length=284, Percent_Identity=30.6338028169014, Blast_Score=120, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6325144, Length=198, Percent_Identity=31.8181818181818, Blast_Score=92, Evalue=8e-20,
Organism=Saccharomyces cerevisiae, GI6324964, Length=240, Percent_Identity=26.25, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6321253, Length=279, Percent_Identity=24.7311827956989, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI45551003, Length=312, Percent_Identity=37.5, Blast_Score=200, Evalue=9e-52,
Organism=Drosophila melanogaster, GI45552429, Length=312, Percent_Identity=37.5, Blast_Score=200, Evalue=1e-51,
Organism=Drosophila melanogaster, GI24585514, Length=312, Percent_Identity=37.5, Blast_Score=200, Evalue=1e-51,
Organism=Drosophila melanogaster, GI28574282, Length=312, Percent_Identity=37.5, Blast_Score=200, Evalue=1e-51,
Organism=Drosophila melanogaster, GI28574284, Length=312, Percent_Identity=37.5, Blast_Score=200, Evalue=1e-51,
Organism=Drosophila melanogaster, GI28574286, Length=311, Percent_Identity=37.620578778135, Blast_Score=196, Evalue=1e-50,
Organism=Drosophila melanogaster, GI28571528, Length=312, Percent_Identity=38.7820512820513, Blast_Score=184, Evalue=7e-47,
Organism=Drosophila melanogaster, GI24585516, Length=247, Percent_Identity=35.6275303643725, Blast_Score=156, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24646446, Length=272, Percent_Identity=33.8235294117647, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24646448, Length=272, Percent_Identity=33.8235294117647, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24646452, Length=272, Percent_Identity=33.8235294117647, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24646450, Length=272, Percent_Identity=33.8235294117647, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI19921140, Length=316, Percent_Identity=30.0632911392405, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI62472511, Length=272, Percent_Identity=33.8235294117647, Blast_Score=128, Evalue=4e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.79; =1.1.1.81 [H]

Molecular weight: Translated: 35433; Mature: 35433

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS00670 D_2_HYDROXYACID_DH_2 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPSVILYKTLPDDLLQRLEEHFSVTQVKNLRPETVSQHAEAFAQAEGLLGSSEKVDAAL
CCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHH
LEKMPKLRATSTVSVGYDNFDVEALNARRVLLMHTPTVLTETVADTVMALVLSTARRVVE
HHHCCCCCCCCEEEECCCCCCEEEECCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHH
VAERVKAGEWTKSIGPDWFGTDVHHKTLGIVGMGRIGMALAQRAHFGFGMPILYNARRQH
HHHHHHCCCCHHHCCCCCCCCCCCHHHCCEEECCHHHHHHHHHHHCCCCCHHEECCHHCC
PQAEERFNARYCDLDTLLQEADFVCLILPLSEETHHLFGQAQFAKMKSSAIFINAGRGPV
CCHHHHHCCCCCCHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHCCCEEEEECCCCCC
VDEQALIAALQNGEIHAAGLDVFEHEPLAKDSPLLSLPNVVALPHIGSATHETRYNMAAC
CCHHHHHHHHCCCCEEEECCCHHCCCCCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHH
AVDNLIDALNGNVEKNCVNPQVK
HHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MKPSVILYKTLPDDLLQRLEEHFSVTQVKNLRPETVSQHAEAFAQAEGLLGSSEKVDAAL
CCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHH
LEKMPKLRATSTVSVGYDNFDVEALNARRVLLMHTPTVLTETVADTVMALVLSTARRVVE
HHHCCCCCCCCEEEECCCCCCEEEECCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHH
VAERVKAGEWTKSIGPDWFGTDVHHKTLGIVGMGRIGMALAQRAHFGFGMPILYNARRQH
HHHHHHCCCCHHHCCCCCCCCCCCHHHCCEEECCHHHHHHHHHHHCCCCCHHEECCHHCC
PQAEERFNARYCDLDTLLQEADFVCLILPLSEETHHLFGQAQFAKMKSSAIFINAGRGPV
CCHHHHHCCCCCCHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHCCCEEEEECCCCCC
VDEQALIAALQNGEIHAAGLDVFEHEPLAKDSPLLSLPNVVALPHIGSATHETRYNMAAC
CCHHHHHHHHCCCCEEEECCCHHCCCCCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHH
AVDNLIDALNGNVEKNCVNPQVK
HHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA