Definition Klebsiella pneumoniae NTUH-K2044 chromosome, complete genome.
Accession NC_012731
Length 5,248,520

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The map label for this gene is yahD [C]

Identifier: 238897003

GI number: 238897003

Start: 4992523

End: 4993989

Strand: Reverse

Name: yahD [C]

Synonym: KP1_5248

Alternate gene names: 238897003

Gene position: 4993989-4992523 (Counterclockwise)

Preceding gene: 238897004

Following gene: 238897000

Centisome position: 95.15

GC content: 60.53

Gene sequence:

>1467_bases
GTGACCTCTTCTTCTTTGATTACCCCTGCTTCCTCGCTTCCCCTGTGCATGAACTTTTCATGGAGAGAAAGGATGAAGGT
GATAAAGGGGCTGGTCCTACTGTGCCTGATGATGCTCGCGGGCTGCCAGTCTGAGGAAGAGACCAGCCAGTTCCTGCTGG
CCTGTAAATACGATGCGCCAGCGACGATCGCAGCCATGCTGGATAACGGCATTGATGTGGATGGTCAGGATAAAACCGGC
TTAAGCGGCCTGATGGTCGCTGCTGCGGAAAATCGTCGCGATGTGGTGGAATTGCTGCTTAAGCGTCGGGCGAAGCCGAA
CCTCCAGACGCGCCAGGGCGTCACGGCGCTAATGCTGGCGGCGGCGCGTGGCAGCGATACCGCGATTATCGGCGATCTTC
TCCAGGCGGGAGCGTCGGTGAATCAGACCTCCATCGACAAGAGTACGGCATTGATGTCCGCCATCTCCGACGGCGGGGAC
GTCAGAAACGACTATCAGCATATTCTGGCGATGAAAAAGCCCGATGCGCCTGTCGAAGAGGAGAGTACGCTGGACAAGAT
AGTTGGCGCAACGGCGGCGAAATCGCTGGCGACGGGCAATCGCGCGCTGATGACTGAAGACATGGCGTTACAACTGGCGC
CCGGCGCGTTTAAGAAGAACGTCGACGAGATCGTGGCGCTGCTCCTCAAGCACGGCGCGGACGTGAAGGCCGTCAACGCG
TCCGGGGAATCGGCCTTTTTCCTCGCTGTCGATCACGCGCGATCGGCGGAAACCATCACGACGTTGGCCAACGCCGGGGC
GGACACCAGCCTGGCGGATAAATCCGGCACCACACCGCTGATGCTGGCGGCGGCGGGGGATGATCCCGATCTGGTGCTGG
CGCTGTCGGCCTCCGGGGTGGAAGTGGATAAGCCGAACCGTGAAGGACTCACGGCGCTGCAGGTGGCGGCGGGTCAGGGG
GCGCCTGCGGTGATCGCGGCGCTGGTGCAGCGCGGCGCAAAGGTGGACCAGCTTTCCGCCAACGATCTCAGCCCGCTGAT
GCTGGCGGTCAAAATGAATAATAAAGCCAATGTCGAGGCGCTGCTTGCGGCTGGCGCCAGCGTCAACCTCAGCAATAAGG
CCGGCTATACGGCGATTGGCTACAGCCGGGCCGGCGAGGTTCGCCAGCTGCTGCTGGCACAGCATGCCGAGCTGAAGGGG
CAGGCCGCGCATATGGCGCAAAGCGAACTGCAGTTTTGTGCTAATGCCTTTGCCGACAAGCTGGCATACAGCGATATCGC
CCGGGCGGTGAATAACGATACCCGCCCGGATATTATGCGTCATCAGCAATCCTGCCCGGAGCTGGGGGAGCTGACGATGC
TACTGGGAGAGTTTACATTCACCCCGGCGGGCGCAACCTATCTCGGCGAGCCGGTGACATGTAAGGTATCCGAATATCGC
AAAACGTTTGAGGTGAACTGCCGCTAA

Upstream 100 bases:

>100_bases
TCTCGGGTCTGTTTTCCTTTCCTGAGCCCAGGATAAGCTAATCCCTCGCCGGCTGACGCCGCGCGAGGGATTTTTTTCGC
ACAGTGGACGTGTCTGGCAG

Downstream 100 bases:

>100_bases
AAACGTTGAGCAACCGGCGATTCGCTGCTCAGGCTGCTGAGTTTCTCGCCGGTTGAGCAATTTTTTGAAAATAAGGCATT
GACGGGTAGGCATCCCAGCA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 488; Mature: 487

Protein sequence:

>488_residues
MTSSSLITPASSLPLCMNFSWRERMKVIKGLVLLCLMMLAGCQSEEETSQFLLACKYDAPATIAAMLDNGIDVDGQDKTG
LSGLMVAAAENRRDVVELLLKRRAKPNLQTRQGVTALMLAAARGSDTAIIGDLLQAGASVNQTSIDKSTALMSAISDGGD
VRNDYQHILAMKKPDAPVEEESTLDKIVGATAAKSLATGNRALMTEDMALQLAPGAFKKNVDEIVALLLKHGADVKAVNA
SGESAFFLAVDHARSAETITTLANAGADTSLADKSGTTPLMLAAAGDDPDLVLALSASGVEVDKPNREGLTALQVAAGQG
APAVIAALVQRGAKVDQLSANDLSPLMLAVKMNNKANVEALLAAGASVNLSNKAGYTAIGYSRAGEVRQLLLAQHAELKG
QAAHMAQSELQFCANAFADKLAYSDIARAVNNDTRPDIMRHQQSCPELGELTMLLGEFTFTPAGATYLGEPVTCKVSEYR
KTFEVNCR

Sequences:

>Translated_488_residues
MTSSSLITPASSLPLCMNFSWRERMKVIKGLVLLCLMMLAGCQSEEETSQFLLACKYDAPATIAAMLDNGIDVDGQDKTG
LSGLMVAAAENRRDVVELLLKRRAKPNLQTRQGVTALMLAAARGSDTAIIGDLLQAGASVNQTSIDKSTALMSAISDGGD
VRNDYQHILAMKKPDAPVEEESTLDKIVGATAAKSLATGNRALMTEDMALQLAPGAFKKNVDEIVALLLKHGADVKAVNA
SGESAFFLAVDHARSAETITTLANAGADTSLADKSGTTPLMLAAAGDDPDLVLALSASGVEVDKPNREGLTALQVAAGQG
APAVIAALVQRGAKVDQLSANDLSPLMLAVKMNNKANVEALLAAGASVNLSNKAGYTAIGYSRAGEVRQLLLAQHAELKG
QAAHMAQSELQFCANAFADKLAYSDIARAVNNDTRPDIMRHQQSCPELGELTMLLGEFTFTPAGATYLGEPVTCKVSEYR
KTFEVNCR
>Mature_487_residues
TSSSLITPASSLPLCMNFSWRERMKVIKGLVLLCLMMLAGCQSEEETSQFLLACKYDAPATIAAMLDNGIDVDGQDKTGL
SGLMVAAAENRRDVVELLLKRRAKPNLQTRQGVTALMLAAARGSDTAIIGDLLQAGASVNQTSIDKSTALMSAISDGGDV
RNDYQHILAMKKPDAPVEEESTLDKIVGATAAKSLATGNRALMTEDMALQLAPGAFKKNVDEIVALLLKHGADVKAVNAS
GESAFFLAVDHARSAETITTLANAGADTSLADKSGTTPLMLAAAGDDPDLVLALSASGVEVDKPNREGLTALQVAAGQGA
PAVIAALVQRGAKVDQLSANDLSPLMLAVKMNNKANVEALLAAGASVNLSNKAGYTAIGYSRAGEVRQLLLAQHAELKGQ
AAHMAQSELQFCANAFADKLAYSDIARAVNNDTRPDIMRHQQSCPELGELTMLLGEFTFTPAGATYLGEPVTCKVSEYRK
TFEVNCR

Specific function: Unknown

COG id: COG0666

COG function: function code R; FOG: Ankyrin repeat

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 7 ANK repeats [H]

Homologues:

Organism=Homo sapiens, GI62988328, Length=370, Percent_Identity=30.5405405405405, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI268607595, Length=370, Percent_Identity=30.5405405405405, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI55741641, Length=328, Percent_Identity=28.6585365853659, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI188595682, Length=367, Percent_Identity=28.6103542234332, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI52426737, Length=367, Percent_Identity=28.6103542234332, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI30425444, Length=294, Percent_Identity=30.6122448979592, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI225543463, Length=351, Percent_Identity=28.2051282051282, Blast_Score=93, Evalue=5e-19,
Organism=Homo sapiens, GI32967601, Length=340, Percent_Identity=29.1176470588235, Blast_Score=93, Evalue=6e-19,
Organism=Homo sapiens, GI225543461, Length=351, Percent_Identity=28.2051282051282, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI157739945, Length=323, Percent_Identity=30.030959752322, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI24233530, Length=338, Percent_Identity=25.1479289940828, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI52426735, Length=323, Percent_Identity=28.7925696594427, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI70780355, Length=330, Percent_Identity=26.3636363636364, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI70780359, Length=330, Percent_Identity=26.3636363636364, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI70780353, Length=330, Percent_Identity=26.3636363636364, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI70780357, Length=330, Percent_Identity=26.3636363636364, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI215598574, Length=330, Percent_Identity=26.3636363636364, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI68131557, Length=323, Percent_Identity=27.8637770897833, Blast_Score=79, Evalue=7e-15,
Organism=Homo sapiens, GI10863929, Length=313, Percent_Identity=28.4345047923323, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI46519147, Length=382, Percent_Identity=29.5811518324607, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI37620163, Length=382, Percent_Identity=29.5811518324607, Blast_Score=76, Evalue=6e-14,
Organism=Homo sapiens, GI38683816, Length=387, Percent_Identity=28.6821705426357, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI38683807, Length=387, Percent_Identity=28.6821705426357, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI89363047, Length=277, Percent_Identity=26.3537906137184, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI157743284, Length=289, Percent_Identity=28.0276816608997, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI13376842, Length=343, Percent_Identity=28.8629737609329, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI304361760, Length=157, Percent_Identity=32.484076433121, Blast_Score=69, Evalue=9e-12,
Organism=Homo sapiens, GI304361757, Length=157, Percent_Identity=32.484076433121, Blast_Score=69, Evalue=9e-12,
Organism=Homo sapiens, GI223634006, Length=332, Percent_Identity=30.421686746988, Blast_Score=69, Evalue=9e-12,
Organism=Homo sapiens, GI121582655, Length=167, Percent_Identity=30.5389221556886, Blast_Score=69, Evalue=9e-12,
Organism=Homo sapiens, GI224809474, Length=217, Percent_Identity=30.4147465437788, Blast_Score=68, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71981411, Length=352, Percent_Identity=27.8409090909091, Blast_Score=92, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI25145251, Length=353, Percent_Identity=28.328611898017, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI71981389, Length=353, Percent_Identity=28.328611898017, Blast_Score=86, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17542700, Length=353, Percent_Identity=28.328611898017, Blast_Score=85, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI71981414, Length=353, Percent_Identity=28.328611898017, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI212646033, Length=273, Percent_Identity=27.4725274725275, Blast_Score=68, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI212646031, Length=273, Percent_Identity=27.4725274725275, Blast_Score=68, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI212646035, Length=273, Percent_Identity=27.4725274725275, Blast_Score=68, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17555340, Length=281, Percent_Identity=28.1138790035587, Blast_Score=65, Evalue=8e-11,
Organism=Drosophila melanogaster, GI281359521, Length=340, Percent_Identity=31.4705882352941, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI281359519, Length=340, Percent_Identity=31.4705882352941, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI28558752, Length=340, Percent_Identity=31.4705882352941, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI28558750, Length=340, Percent_Identity=31.4705882352941, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI28558756, Length=340, Percent_Identity=31.4705882352941, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI28558754, Length=340, Percent_Identity=31.4705882352941, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI161082092, Length=365, Percent_Identity=28.7671232876712, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI161082096, Length=356, Percent_Identity=28.3707865168539, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI161082110, Length=356, Percent_Identity=28.3707865168539, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI161082081, Length=356, Percent_Identity=28.3707865168539, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI221330474, Length=342, Percent_Identity=28.6549707602339, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI221330482, Length=342, Percent_Identity=28.6549707602339, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI161082106, Length=356, Percent_Identity=28.3707865168539, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI221330480, Length=342, Percent_Identity=28.6549707602339, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI28573593, Length=342, Percent_Identity=28.6549707602339, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI281363919, Length=342, Percent_Identity=28.6549707602339, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI221330478, Length=342, Percent_Identity=28.6549707602339, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI221330476, Length=342, Percent_Identity=28.6549707602339, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI45550479, Length=342, Percent_Identity=28.6549707602339, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI161082089, Length=373, Percent_Identity=29.2225201072386, Blast_Score=87, Evalue=4e-17,
Organism=Drosophila melanogaster, GI45551135, Length=342, Percent_Identity=28.6549707602339, Blast_Score=87, Evalue=4e-17,
Organism=Drosophila melanogaster, GI161082085, Length=373, Percent_Identity=29.2225201072386, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI161082099, Length=373, Percent_Identity=29.2225201072386, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24650843, Length=357, Percent_Identity=26.6106442577031, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI45552223, Length=389, Percent_Identity=25.1928020565553, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI221472807, Length=389, Percent_Identity=25.1928020565553, Blast_Score=75, Evalue=9e-14,
Organism=Drosophila melanogaster, GI17998549, Length=391, Percent_Identity=25.0639386189258, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24663095, Length=345, Percent_Identity=26.3768115942029, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI21357681, Length=345, Percent_Identity=26.3768115942029, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24663102, Length=345, Percent_Identity=26.3768115942029, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24663106, Length=345, Percent_Identity=26.3768115942029, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24663091, Length=345, Percent_Identity=26.0869565217391, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI28571867, Length=360, Percent_Identity=28.3333333333333, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI28571865, Length=360, Percent_Identity=28.3333333333333, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002110
- InterPro:   IPR020683 [H]

Pfam domain/function: PF00023 Ank [H]

EC number: NA

Molecular weight: Translated: 51312; Mature: 51181

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS50088 ANK_REPEAT ; PS50297 ANK_REP_REGION

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure: 

>Translated Secondary Structure
MTSSSLITPASSLPLCMNFSWRERMKVIKGLVLLCLMMLAGCQSEEETSQFLLACKYDAP
CCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHEEEEEECCCC
ATIAAMLDNGIDVDGQDKTGLSGLMVAAAENRRDVVELLLKRRAKPNLQTRQGVTALMLA
HHHHHHHCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCHHHHCHHHHHHH
AARGSDTAIIGDLLQAGASVNQTSIDKSTALMSAISDGGDVRNDYQHILAMKKPDAPVEE
HCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCH
ESTLDKIVGATAAKSLATGNRALMTEDMALQLAPGAFKKNVDEIVALLLKHGADVKAVNA
HHHHHHHHHHHHHHHHHCCCCEEEECCCEEEECCCHHHCCHHHHHHHHHHCCCCEEEEEC
SGESAFFLAVDHARSAETITTLANAGADTSLADKSGTTPLMLAAAGDDPDLVLALSASGV
CCCCEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCEEEEEECCCC
EVDKPNREGLTALQVAAGQGAPAVIAALVQRGAKVDQLSANDLSPLMLAVKMNNKANVEA
CCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCCCCHHH
LLAAGASVNLSNKAGYTAIGYSRAGEVRQLLLAQHAELKGQAAHMAQSELQFCANAFADK
HHHCCCCEEECCCCCCEEECCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
LAYSDIARAVNNDTRPDIMRHQQSCPELGELTMLLGEFTFTPAGATYLGEPVTCKVSEYR
HHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHCCCCCEEEHHHCC
KTFEVNCR
CCEEECCC
>Mature Secondary Structure 
TSSSLITPASSLPLCMNFSWRERMKVIKGLVLLCLMMLAGCQSEEETSQFLLACKYDAP
CCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHEEEEEECCCC
ATIAAMLDNGIDVDGQDKTGLSGLMVAAAENRRDVVELLLKRRAKPNLQTRQGVTALMLA
HHHHHHHCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCHHHHCHHHHHHH
AARGSDTAIIGDLLQAGASVNQTSIDKSTALMSAISDGGDVRNDYQHILAMKKPDAPVEE
HCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCH
ESTLDKIVGATAAKSLATGNRALMTEDMALQLAPGAFKKNVDEIVALLLKHGADVKAVNA
HHHHHHHHHHHHHHHHHCCCCEEEECCCEEEECCCHHHCCHHHHHHHHHHCCCCEEEEEC
SGESAFFLAVDHARSAETITTLANAGADTSLADKSGTTPLMLAAAGDDPDLVLALSASGV
CCCCEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCEEEEEECCCC
EVDKPNREGLTALQVAAGQGAPAVIAALVQRGAKVDQLSANDLSPLMLAVKMNNKANVEA
CCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCCCCHHH
LLAAGASVNLSNKAGYTAIGYSRAGEVRQLLLAQHAELKGQAAHMAQSELQFCANAFADK
HHHCCCCEEECCCCCCEEECCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
LAYSDIARAVNNDTRPDIMRHQQSCPELGELTMLLGEFTFTPAGATYLGEPVTCKVSEYR
HHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHCCCCCEEEHHHCC
KTFEVNCR
CCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12125824 [H]