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The map label for this gene is yhjW [H]

Identifier: 238897004

GI number: 238897004

Start: 4994109

End: 4995782

Strand: Reverse

Name: yhjW [H]

Synonym: KP1_5249

Alternate gene names: 238897004

Gene position: 4995782-4994109 (Counterclockwise)

Preceding gene: 238897005

Following gene: 238897003

Centisome position: 95.18

GC content: 56.93

Gene sequence:

>1674_bases
ATGAAATATATTAGAACGATGACGCAGCAGAAGCTTAGTTTTTGGCTGGCGCTGTACATCGGCTGGTTTATGAACGTCGC
CGTTTTTTTCCGGCGTTTCGATGGTTATGCTCAAGAGTTCACTTTCTGGAAAGGGCTTTCCGGTGTTGTTGAACTGGTTG
CCACGGTATTTGTCACCTTCTTCCTGTTACGTCTTCTGTCGCTGTTCGGCCGCCGTATCTGGCGCATTCTGGCGACGCTG
ATTGTCCTGTTTTCCGCCGCGGCGAGTTACTACATGACGTTCCTCAATGTGGTGATTGGCTACGGGATTATCGCTTCGGT
GATGACCACCGATATCGACCTGTCGAAAGAGGTCATCGGCTGGCACCTGATCCTCTGGCTGGTGGCGGTGAGCGCGCCGC
CGTTGCTGTTCATCTGGAGCAACCGCTGCCGCCATACGCTGCTGCGCCAGCTGCGCACCCCGGGCCAGCGGGTTAAAAAC
GTGCTGATCGTGGTGCTGGCCGGACTGATTGTCTGGGGACCCATCCGCCTGCTAGAGCTGCGCCAGCATGATGTGGAGCG
CCATTCGGAAGTGGATATGCCGAGCTATGGTGGGGTGATCGCCAACTCTTACCTGCCGTCGAACTGGTTGTCGGCGCTGG
GTCTGTATGCCTGGGCGCAGGTGGATGAATCCTCAGACAACAAATCGCTGATTAACCCGGCGAAGAAGTTCACCTACGTC
GCGCCGGAAGGTCTGGATGATACCTACGTGGTGTTTATCATTGGCGAAACGACCCGCTGGGACCATATGGGCATCCTCGG
CTATAGCCGCAATACCACGCCAGAGCTGGAGAAAGAGAAGAATCTCGTCGCCTTCCGCGGTTACTCGTGCGATACCGCTA
CCAAACTGTCGTTACGCTGCATGTTTGTGCGCGAGGGCGGGGCGGAAGATAACCCACAGCGGACGCTCAAAGAGCAGAAT
GTCTTTGCCGTGCTGCATCAGCTGGGCTTCAGCGGCAATCTGTACGCCATGCAGAGCGAGATGTGGTTCTACAGCAACAC
GATGGCCAACAATATCGCCTACCGCGAGCAGATTGGCGCCGAGCCGCGCAACCGCGGTAAGAGCGTTGACGATATGCTGC
TGGTGGATGAGATGAAGCGCGGTATGGCGCAGGGCAACGCCTCCGGTAAGCATCTGATCATTCTCCACACCAAAGGCTCC
CACTTTAACTACACCCAACGCTACCCGCGCAGCTTCGCCCAGTGGAAACCGGAGTGCGTCGGCGTCGACAACAAGTGCTC
GAAAGCGGAACTGATCAATTCCTACGACAATAGCGTGACCTATGTCGATCACTTTATCGTCAGCGTCCTCGACCAGCTGC
GGGATAAGAAAGCGATTGTGTTCTATGCCGCCGACCACGGGGAGTCGATTAACGAGCGTGAACACCTGCACGGTACGCCG
CGCAAGATGGCGCCGCCGGAGCAGTTCCGCGTGCCGATGATGGTGTGGATGTCGGATAAGTACCTGGAAAATCCCGATCA
CGCCGCCGCGTTTGCCCATCTGCAGCAGCAGGCTGCGATGAAGGTGCCGCGCCGTCACGTCGAGCTGTACGACACCATTA
TGGGCTGCCTCGGCTATACCTCGCCGGATGGCGGGATCAATGAGAACAACAACTGGTGCCGGTGGAAAAAGTAA

Upstream 100 bases:

>100_bases
AGAGGTTTCTGATAACCAACCCTAACGCACCCTATTAGCCTACTGACTCGTGCCACAGCGTCAGGCGGTGTTTTATTCTT
CTTTTTCCAGGTTTGCTTGC

Downstream 100 bases:

>100_bases
GGCCCGATAGTTTTCAGATTCTCGGGTCTGTTTTCCTTTCCTGAGCCCAGGATAAGCTAATCCCTCGCCGGCTGACGCCG
CGCGAGGGATTTTTTTCGCA

Product: phosphoethanolamine transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 557; Mature: 557

Protein sequence:

>557_residues
MKYIRTMTQQKLSFWLALYIGWFMNVAVFFRRFDGYAQEFTFWKGLSGVVELVATVFVTFFLLRLLSLFGRRIWRILATL
IVLFSAAASYYMTFLNVVIGYGIIASVMTTDIDLSKEVIGWHLILWLVAVSAPPLLFIWSNRCRHTLLRQLRTPGQRVKN
VLIVVLAGLIVWGPIRLLELRQHDVERHSEVDMPSYGGVIANSYLPSNWLSALGLYAWAQVDESSDNKSLINPAKKFTYV
APEGLDDTYVVFIIGETTRWDHMGILGYSRNTTPELEKEKNLVAFRGYSCDTATKLSLRCMFVREGGAEDNPQRTLKEQN
VFAVLHQLGFSGNLYAMQSEMWFYSNTMANNIAYREQIGAEPRNRGKSVDDMLLVDEMKRGMAQGNASGKHLIILHTKGS
HFNYTQRYPRSFAQWKPECVGVDNKCSKAELINSYDNSVTYVDHFIVSVLDQLRDKKAIVFYAADHGESINEREHLHGTP
RKMAPPEQFRVPMMVWMSDKYLENPDHAAAFAHLQQQAAMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCRWKK

Sequences:

>Translated_557_residues
MKYIRTMTQQKLSFWLALYIGWFMNVAVFFRRFDGYAQEFTFWKGLSGVVELVATVFVTFFLLRLLSLFGRRIWRILATL
IVLFSAAASYYMTFLNVVIGYGIIASVMTTDIDLSKEVIGWHLILWLVAVSAPPLLFIWSNRCRHTLLRQLRTPGQRVKN
VLIVVLAGLIVWGPIRLLELRQHDVERHSEVDMPSYGGVIANSYLPSNWLSALGLYAWAQVDESSDNKSLINPAKKFTYV
APEGLDDTYVVFIIGETTRWDHMGILGYSRNTTPELEKEKNLVAFRGYSCDTATKLSLRCMFVREGGAEDNPQRTLKEQN
VFAVLHQLGFSGNLYAMQSEMWFYSNTMANNIAYREQIGAEPRNRGKSVDDMLLVDEMKRGMAQGNASGKHLIILHTKGS
HFNYTQRYPRSFAQWKPECVGVDNKCSKAELINSYDNSVTYVDHFIVSVLDQLRDKKAIVFYAADHGESINEREHLHGTP
RKMAPPEQFRVPMMVWMSDKYLENPDHAAAFAHLQQQAAMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCRWKK
>Mature_557_residues
MKYIRTMTQQKLSFWLALYIGWFMNVAVFFRRFDGYAQEFTFWKGLSGVVELVATVFVTFFLLRLLSLFGRRIWRILATL
IVLFSAAASYYMTFLNVVIGYGIIASVMTTDIDLSKEVIGWHLILWLVAVSAPPLLFIWSNRCRHTLLRQLRTPGQRVKN
VLIVVLAGLIVWGPIRLLELRQHDVERHSEVDMPSYGGVIANSYLPSNWLSALGLYAWAQVDESSDNKSLINPAKKFTYV
APEGLDDTYVVFIIGETTRWDHMGILGYSRNTTPELEKEKNLVAFRGYSCDTATKLSLRCMFVREGGAEDNPQRTLKEQN
VFAVLHQLGFSGNLYAMQSEMWFYSNTMANNIAYREQIGAEPRNRGKSVDDMLLVDEMKRGMAQGNASGKHLIILHTKGS
HFNYTQRYPRSFAQWKPECVGVDNKCSKAELINSYDNSVTYVDHFIVSVLDQLRDKKAIVFYAADHGESINEREHLHGTP
RKMAPPEQFRVPMMVWMSDKYLENPDHAAAFAHLQQQAAMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCRWKK

Specific function: Catalyzes the addition of a phosphoethanolamine (pEtN) moiety to the outer 3-deoxy-D-manno-octulosonic acid (KDO) residue of a KDO(2)-lipid A. Phosphatidylethanolamines with one unsaturated acyl group functions as pEtN donors and the reaction releases dia

COG id: COG2194

COG function: function code R; Predicted membrane-associated, metal-dependent hydrolase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoethanolamine transferase family. EptB subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082286, Length=553, Percent_Identity=82.0976491862568, Blast_Score=951, Evalue=0.0,
Organism=Escherichia coli, GI87082372, Length=309, Percent_Identity=31.0679611650485, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI87082223, Length=491, Percent_Identity=25.050916496945, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI1790392, Length=519, Percent_Identity=23.3140655105973, Blast_Score=83, Evalue=6e-17,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR017850
- InterPro:   IPR012549
- InterPro:   IPR000917 [H]

Pfam domain/function: PF08019 DUF1705; PF00884 Sulfatase [H]

EC number: NA

Molecular weight: Translated: 63845; Mature: 63845

Theoretical pI: Translated: 8.85; Mature: 8.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCCCCCEECCCC
>Mature Secondary Structure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CCCCCCCCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677609; 7721701 [H]