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The map label for this gene is dctA [H]

Identifier: 238896976

GI number: 238896976

Start: 4954678

End: 4955964

Strand: Reverse

Name: dctA [H]

Synonym: KP1_5219

Alternate gene names: 238896976

Gene position: 4955964-4954678 (Counterclockwise)

Preceding gene: 238896977

Following gene: 238896975

Centisome position: 94.43

GC content: 56.33

Gene sequence:

>1287_bases
ATGAAAACCTCTATCTTTAAAAGCCTCTATGTCCAGGTTCTGACGGCCATAGCGATCGGTATTCTGCTGGGCCATTTTTA
CCCGGAACTGGGCGCGCAAATGAAACCCTTCGGCGACGCCTTCGTTAAGCTGATTAAAATGGTGATTGCTCCGGTGATCT
TCTGTACCGTCGTGACGGGGATCGCCGGGATGGAAAGCATGAAAGCCGTTGGCCGTACCGGGGCGGTGGCGTTGCTCTAT
TTCGAAGTGGTCAGCACCATCGCGCTGATTATCGGTCTGATTATCGTCAACGTGGTGCAGCCGGGCGCCGGGATGAACGT
CGACCCGTCGACGCTCGATGCGAAAGCGGTGGCGGTGTACGCTGAGCAGGCTAAAGATCAAGGGGTAGTGGCCTTTTTAC
TGGATGTGATCCCGGGCAGCGTCATTGGCGCCTTCGCCAGCGGCAACATTCTGCAGGTTCTGCTGTTCGCCGTCCTGTTT
GGTTTTGCCCTGCACCGTCTGGGCAGCAAGGGCCAGCTGATTTTCAACGTCATTGAAAGCTTCTCCCAGGTTATCTTCGG
CATTATCAATATGATCATGCGCCTGGCGCCGATCGGCGCCTTCGGAGCCATGGCCTTTACCATCGGTAAATATGGCGTCG
GCACCCTGGTGCAGCTGGGGCAGCTGATCATCTGCTTCTATATCACTTGTATCCTGTTCGTGGTGGTGGTGCTGGGGAGC
ATCGCCCGGGCCACCGGCTTCAGCATCTTCAAATTTATCCGCTATATCCGGGAAGAGCTGCTGATTGTGCTGGGGACCTC
CTCATCAGAATCGGCGCTGCCGAGAATGCTCGACAAGATGGAGAAGCTGGGCTGCCGTAAATCGGTGGTAGGCCTGGTGA
TCCCGACCGGCTATTCGTTCAACCTCGATGGCACCTCTATCTATCTGACGATGGCCGCAGTCTTTATCGCCCAGGCGACC
AACAGCCATATGGATATTTTCCATCAGATCACCCTGCTGGTGGTGCTGTTGCTCTCCTCGAAAGGGGCGGCAGGGGTGAC
CGGCAGCGGCTTCATCGTGCTGGCGGCGACCATCTCGGCCGTCGGTCATTTACCGGTGGCTGGTCTGGCGCTGATCCTCG
GTATCGACCGCTTTATGTCCGAAGCCCGCGCGCTGACTAACCTGGTGGGTAACGGCGTTGCCACGGTGGTGGTGGCGAAA
TGGGTGAAAGAACTGGATGCCAAACAGATGGATGACGTTCTGAATAACCGTGTTCCGGCGAACAAAACGCACGAATTATC
CTCTTAA

Upstream 100 bases:

>100_bases
TCGGCGTTAGTTATTTCTGAAATGTTTCCAGGGTGTTATTTTTAAGCCACAGACGTCAAATATCCCTTCTTGCTCTGTGG
TTATTCCCAAAGGACACCCT

Downstream 100 bases:

>100_bases
ATCCTCCTCTTAGGCCCGTACTCCCTTGCTGGAGTACGGCCTCCTGCGCATAATTAGAGGTCATTCCCGCCCGGCGGGAA
TGACACGAAATGATTATTTT

Product: C4-dicarboxylate transporter DctA

Products: orotate [Cytoplasm]; fumarate [Cytoplasm]; malate [Cytoplasm]; Na (I) [Cytoplasm]; succinate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MKTSIFKSLYVQVLTAIAIGILLGHFYPELGAQMKPFGDAFVKLIKMVIAPVIFCTVVTGIAGMESMKAVGRTGAVALLY
FEVVSTIALIIGLIIVNVVQPGAGMNVDPSTLDAKAVAVYAEQAKDQGVVAFLLDVIPGSVIGAFASGNILQVLLFAVLF
GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQLGQLIICFYITCILFVVVVLGS
IARATGFSIFKFIRYIREELLIVLGTSSSESALPRMLDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQAT
NSHMDIFHQITLLVVLLLSSKGAAGVTGSGFIVLAATISAVGHLPVAGLALILGIDRFMSEARALTNLVGNGVATVVVAK
WVKELDAKQMDDVLNNRVPANKTHELSS

Sequences:

>Translated_428_residues
MKTSIFKSLYVQVLTAIAIGILLGHFYPELGAQMKPFGDAFVKLIKMVIAPVIFCTVVTGIAGMESMKAVGRTGAVALLY
FEVVSTIALIIGLIIVNVVQPGAGMNVDPSTLDAKAVAVYAEQAKDQGVVAFLLDVIPGSVIGAFASGNILQVLLFAVLF
GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQLGQLIICFYITCILFVVVVLGS
IARATGFSIFKFIRYIREELLIVLGTSSSESALPRMLDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQAT
NSHMDIFHQITLLVVLLLSSKGAAGVTGSGFIVLAATISAVGHLPVAGLALILGIDRFMSEARALTNLVGNGVATVVVAK
WVKELDAKQMDDVLNNRVPANKTHELSS
>Mature_428_residues
MKTSIFKSLYVQVLTAIAIGILLGHFYPELGAQMKPFGDAFVKLIKMVIAPVIFCTVVTGIAGMESMKAVGRTGAVALLY
FEVVSTIALIIGLIIVNVVQPGAGMNVDPSTLDAKAVAVYAEQAKDQGVVAFLLDVIPGSVIGAFASGNILQVLLFAVLF
GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQLGQLIICFYITCILFVVVVLGS
IARATGFSIFKFIRYIREELLIVLGTSSSESALPRMLDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQAT
NSHMDIFHQITLLVVLLLSSKGAAGVTGSGFIVLAATISAVGHLPVAGLALILGIDRFMSEARALTNLVGNGVATVVVAK
WVKELDAKQMDDVLNNRVPANKTHELSS

Specific function: Responsible for the transport of dicarboxylates such as succinate, fumarate, and malate from the periplasm across the membrane [H]

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI5032093, Length=401, Percent_Identity=28.6783042394015, Blast_Score=132, Evalue=5e-31,
Organism=Homo sapiens, GI40254478, Length=455, Percent_Identity=26.1538461538462, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI169790839, Length=479, Percent_Identity=26.7223382045929, Blast_Score=125, Evalue=7e-29,
Organism=Homo sapiens, GI21314632, Length=444, Percent_Identity=25.9009009009009, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI223468566, Length=270, Percent_Identity=31.8518518518519, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI223468564, Length=270, Percent_Identity=31.8518518518519, Blast_Score=122, Evalue=8e-28,
Organism=Homo sapiens, GI4827012, Length=389, Percent_Identity=26.7352185089974, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI66773030, Length=425, Percent_Identity=25.1764705882353, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI194239697, Length=465, Percent_Identity=23.4408602150538, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI262359914, Length=413, Percent_Identity=26.1501210653753, Blast_Score=92, Evalue=8e-19,
Organism=Homo sapiens, GI301601644, Length=189, Percent_Identity=27.5132275132275, Blast_Score=79, Evalue=1e-14,
Organism=Escherichia coli, GI1789947, Length=428, Percent_Identity=93.6915887850467, Blast_Score=807, Evalue=0.0,
Organism=Escherichia coli, GI1790514, Length=416, Percent_Identity=39.9038461538462, Blast_Score=318, Evalue=6e-88,
Organism=Escherichia coli, GI1788024, Length=397, Percent_Identity=21.6624685138539, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1789473, Length=254, Percent_Identity=25.5905511811024, Blast_Score=73, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17537407, Length=397, Percent_Identity=24.9370277078086, Blast_Score=138, Evalue=7e-33,
Organism=Caenorhabditis elegans, GI71983106, Length=463, Percent_Identity=25.2699784017279, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI71983099, Length=463, Percent_Identity=25.2699784017279, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI71996953, Length=419, Percent_Identity=24.8210023866348, Blast_Score=115, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI193206654, Length=470, Percent_Identity=22.7659574468085, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI193206505, Length=452, Percent_Identity=22.787610619469, Blast_Score=104, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI17541374, Length=443, Percent_Identity=24.3792325056433, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24583025, Length=425, Percent_Identity=23.0588235294118, Blast_Score=108, Evalue=8e-24,
Organism=Drosophila melanogaster, GI17137668, Length=425, Percent_Identity=23.0588235294118, Blast_Score=108, Evalue=8e-24,
Organism=Drosophila melanogaster, GI24583023, Length=425, Percent_Identity=23.0588235294118, Blast_Score=108, Evalue=8e-24,
Organism=Drosophila melanogaster, GI281360481, Length=457, Percent_Identity=24.2888402625821, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI281360483, Length=457, Percent_Identity=24.2888402625821, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI17137666, Length=457, Percent_Identity=24.2888402625821, Blast_Score=87, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR018107 [H]

Pfam domain/function: PF00375 SDF [H]

EC number: NA

Molecular weight: Translated: 45371; Mature: 45371

Theoretical pI: Translated: 9.28; Mature: 9.28

Prosite motif: PS00713 NA_DICARBOXYL_SYMP_1 ; PS00714 NA_DICARBOXYL_SYMP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTSIFKSLYVQVLTAIAIGILLGHFYPELGAQMKPFGDAFVKLIKMVIAPVIFCTVVTG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
IAGMESMKAVGRTGAVALLYFEVVSTIALIIGLIIVNVVQPGAGMNVDPSTLDAKAVAVY
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHH
AEQAKDQGVVAFLLDVIPGSVIGAFASGNILQVLLFAVLFGFALHRLGSKGQLIFNVIES
HHHCCCCHHHHHHHHHHCHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
FSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQLGQLIICFYITCILFVVVVLGS
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IARATGFSIFKFIRYIREELLIVLGTSSSESALPRMLDKMEKLGCRKSVVGLVIPTGYSF
HHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHCCHHHHHEEEEECCEEE
NLDGTSIYLTMAAVFIAQATNSHMDIFHQITLLVVLLLSSKGAAGVTGSGFIVLAATISA
CCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
VGHLPVAGLALILGIDRFMSEARALTNLVGNGVATVVVAKWVKELDAKQMDDVLNNRVPA
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
NKTHELSS
CCCCCCCC
>Mature Secondary Structure
MKTSIFKSLYVQVLTAIAIGILLGHFYPELGAQMKPFGDAFVKLIKMVIAPVIFCTVVTG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
IAGMESMKAVGRTGAVALLYFEVVSTIALIIGLIIVNVVQPGAGMNVDPSTLDAKAVAVY
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHH
AEQAKDQGVVAFLLDVIPGSVIGAFASGNILQVLLFAVLFGFALHRLGSKGQLIFNVIES
HHHCCCCHHHHHHHHHHCHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
FSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQLGQLIICFYITCILFVVVVLGS
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IARATGFSIFKFIRYIREELLIVLGTSSSESALPRMLDKMEKLGCRKSVVGLVIPTGYSF
HHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHCCHHHHHEEEEECCEEE
NLDGTSIYLTMAAVFIAQATNSHMDIFHQITLLVVLLLSSKGAAGVTGSGFIVLAATISA
CCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
VGHLPVAGLALILGIDRFMSEARALTNLVGNGVATVVVAKWVKELDAKQMDDVLNNRVPA
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
NKTHELSS
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: orotate [Periplasm]; fumarate [Periplasm]; malate [Periplasm]; Na (I) [Periplasm]; succinate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + orotate [Periplasm] = Na (I) [Cytoplasm] + orotate [Cytoplasm] Na (I) [Periplasm] + fumarate [Periplasm] = Na (I) [Cytoplasm] + fumarate [Cytoplasm] Na (I) [Periplasm] + malate [Periplasm] = Na (I) [Cytoplasm] + malate [Cytoplasm] Na

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA