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The map label for this gene is rpoN [H]

Identifier: 238896709

GI number: 238896709

Start: 4684640

End: 4686073

Strand: Direct

Name: rpoN [H]

Synonym: KP1_4924

Alternate gene names: 238896709

Gene position: 4684640-4686073 (Clockwise)

Preceding gene: 238896708

Following gene: 238896710

Centisome position: 89.26

GC content: 56.35

Gene sequence:

>1434_bases
ATGAAGCAAGGTTTGCAATTAAGGCTTAGTCAACAGCTTGCCATGACGCCGCAGCTGCAGCAGGCGATTCGTTTGCTGCA
GTTGTCCACGTTAGAACTCCAGCAAGAGCTCCAGCAAGCGCTGGAAAGTAACCCGTTGCTTGAGCAAACCGATCTTCACG
ACGAGGTAGAGGCAAAAGAGGTGGAGGACCGCGAGTCCCTCGATACCGTCGATGCGCTTGAGCAAAAAGAGATGCCCGAC
GAACTGCCTCTCGATGCCAGCTGGGATGAAATCTATACCGCTGGCACGCCGTCGGGTAACGGCGTCGACTATCAGGACGA
CGAGCTTCCCGTCTATCAAGGCGAGACCACCCAGACGCTGCAGGACTATCTGATGTGGCAGGTTGAGCTGACGCCTTTCA
CCGACACCGACCGCGCTATTGCCACCTCTATCGTCGACGCCGTCGACGATACGGGGTACCTCACGATTCAGATTGAAGAT
ATTGTCGACAGCATCGGCGATGACGAGATCGGCCTGGAGGAGGTGGAAGCGGTCCTCAAGCGAATCCAGCGTTTTGATCC
GGTTGGCGTCGCAGCGAAAGATCTGCGCGACTGCCTGCTGATCCAGCTGTCGCAATTTGCTAAAGAGACGCCGTGGCTGG
AAGAGGCCCGGCTGATTATCAGCGACCATCTTGATCTGCTGGCCAACCATGATTTCCGCACTCTAATGCGAGTCACCCGC
CTGAAAGAAGAGGTGCTTAAAGAGGCGGTCAACCTAATCCAGTCCCTGGATCCTCGCCCTGGCCAGTCCATTCAGACCAG
CGAACCGGAATATGTCATCCCGGATGTGCTGGTGCGCAAGGTGAGCGGCCGCTGGACCGTCGAGCTCAACGCCGACAGCA
TTCCACGGTTGAAAATAAATCAGCAGTATGCGGCAATGGGCAACAGCGCCCGCAATGACGCCGACGGCCAGTTCATTCGC
AGCAATCTGCAGGAAGCCCGCTGGCTGATTAAAAGTCTGGAAAGCCGCAACGATACCCTGCTGCGCGTCAGCCGCTGCAT
CGTAGAGCAGCAGCAGGCTTTTTTCGAACAGGGTGAAGAGTATATGAAGCCGATGGTGCTGGCCGATATCGCCCAGGCCG
TCGAGATGCATGAATCGACGATTTCCCGTGTCACCACGCAAAAATATCTGCACAGTCCGCGCGGTATTTTTGAACTGAAG
TATTTCTTCTCCAGCCATGTGAATACCGAAGGCGGAGGCGAAGCCTCGTCGACGGCTATTCGCGCGCTGGTGAAGAAGCT
CATCGCCGCGGAAAACCCCGCGAAGCCGTTGAGCGACAGTAAGCTCACCTCCATGCTGTCCGAGCAGGGTATTATGGTGG
CGCGCCGCACTGTCGCGAAGTACAGAGAGTCTTTATCCATCCCGCCGTCAAACCAGCGTAAACAACTGGTCTGA

Upstream 100 bases:

>100_bases
TCGAAGATGAGCAGGTTAAGCGCGTGTATCTTGGGGAAGACTTCAGACTCTGATAGGGTAGAGGTTACAGACGTTTTAGC
CGGAGATATTTACCCTGAAC

Downstream 100 bases:

>100_bases
CATAACCGATAAGGAAGACACTATGCAGCTCAACATTACAGGACACAACGTCGAAATTACGCCTGCGATGCGCGAATTCG
TTACGGCGAAGTTCAGCAAG

Product: RNA polymerase factor sigma-54

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQTDLHDEVEAKEVEDRESLDTVDALEQKEMPD
ELPLDASWDEIYTAGTPSGNGVDYQDDELPVYQGETTQTLQDYLMWQVELTPFTDTDRAIATSIVDAVDDTGYLTIQIED
IVDSIGDDEIGLEEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFAKETPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKVSGRWTVELNADSIPRLKINQQYAAMGNSARNDADGQFIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSMLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Sequences:

>Translated_477_residues
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQTDLHDEVEAKEVEDRESLDTVDALEQKEMPD
ELPLDASWDEIYTAGTPSGNGVDYQDDELPVYQGETTQTLQDYLMWQVELTPFTDTDRAIATSIVDAVDDTGYLTIQIED
IVDSIGDDEIGLEEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFAKETPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKVSGRWTVELNADSIPRLKINQQYAAMGNSARNDADGQFIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSMLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV
>Mature_477_residues
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQTDLHDEVEAKEVEDRESLDTVDALEQKEMPD
ELPLDASWDEIYTAGTPSGNGVDYQDDELPVYQGETTQTLQDYLMWQVELTPFTDTDRAIATSIVDAVDDTGYLTIQIED
IVDSIGDDEIGLEEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFAKETPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKVSGRWTVELNADSIPRLKINQQYAAMGNSARNDADGQFIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSMLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of the nitrogen fixation genes. The open complex (sigma-54 and core

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=477, Percent_Identity=90.146750524109, Blast_Score=886, Evalue=0.0,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 53926; Mature: 53926

Theoretical pI: Translated: 4.35; Mature: 4.35

Prosite motif: PS00717 SIGMA54_1 ; PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQTDLHDEVEAKE
CCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCHHHHHHH
VEDRESLDTVDALEQKEMPDELPLDASWDEIYTAGTPSGNGVDYQDDELPVYQGETTQTL
HHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHEECCCCCCCCCCCCCCCCCCCCCCHHHHH
QDYLMWQVELTPFTDTDRAIATSIVDAVDDTGYLTIQIEDIVDSIGDDEIGLEEVEAVLK
HHHHEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEEHHHHHHCCCCCCCHHHHHHHHH
RIQRFDPVGVAAKDLRDCLLIQLSQFAKETPWLEEARLIISDHLDLLANHDFRTLMRVTR
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHH
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKVSGRWTVELNADSIPRLKIN
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECHHHHHHHCCCCEEEEECCCCCCCEEEC
QQYAAMGNSARNDADGQFIRSNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEE
CHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHH
YMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELKYFFSSHVNTEGGGEASSTAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCHHHHHH
RALVKKLIAAENPAKPLSDSKLTSMLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV
HHHHHHHHHCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQTDLHDEVEAKE
CCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCHHHHHHH
VEDRESLDTVDALEQKEMPDELPLDASWDEIYTAGTPSGNGVDYQDDELPVYQGETTQTL
HHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHEECCCCCCCCCCCCCCCCCCCCCCHHHHH
QDYLMWQVELTPFTDTDRAIATSIVDAVDDTGYLTIQIEDIVDSIGDDEIGLEEVEAVLK
HHHHEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEEHHHHHHCCCCCCCHHHHHHHHH
RIQRFDPVGVAAKDLRDCLLIQLSQFAKETPWLEEARLIISDHLDLLANHDFRTLMRVTR
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHH
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKVSGRWTVELNADSIPRLKIN
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECHHHHHHHCCCCEEEEECCCCCCCEEEC
QQYAAMGNSARNDADGQFIRSNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEE
CHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHH
YMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELKYFFSSHVNTEGGGEASSTAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCHHHHHH
RALVKKLIAAENPAKPLSDSKLTSMLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV
HHHHHHHHHCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2999700; 2695747 [H]