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The map label for this gene is fruA [H]

Identifier: 238896644

GI number: 238896644

Start: 4623545

End: 4624957

Strand: Direct

Name: fruA [H]

Synonym: KP1_4849

Alternate gene names: 238896644

Gene position: 4623545-4624957 (Clockwise)

Preceding gene: 238896643

Following gene: 238896645

Centisome position: 88.09

GC content: 54.42

Gene sequence:

>1413_bases
ATGAAAAAAATTATTGCCGTGACCGGCTGCCCTACAGGTATTGCTCATACATTTATGGCTGAAGAGGCCTTAAAAACCGC
AGCGAAAAAATTAAACGTTGAAATAAAAGTCGAAACCAATGGCGCTTCCGGCATAGAAAACGCCATTACGCCGGAAGACC
TGAAGGATATTTATGGCGTCATTATCGCTGCCGATAAAGATGTTAATGCCGAACGTTTTAATGGTTTACCGGTTATTGAG
GTGCCGGTTAAAGAAGCCATTCACCATCCGGCGGAATTAATTAATAAAGTCGTTAACGGTCAGGCGCCGCGTCGTCAGGG
ATTCTCCGCGGCAACGGATCCAGACGAGAAATATGAGCGAGAATCGTTCGGCCGACAGATTTACAAACATCTGATGAGCG
GCGTTTCCAACATGCTACCCTTCGTTGTCGCCGGCGGGATCTTAATCGCTGTTTCTTTCTTATGGGGTATCTACTCCGCC
GACCCGAATTCACCGCAGTATAACGTGATTGCCGCCACGCTGATGAAAGTCGGTCAGCAGGCGTTCTCCATCATGGTGCC
GGTGTTTACCGCCTATATTGCCTGGTCGATATCTGGCCGCCCTGGCATGGTTGCCGGTTTTGTCGGCGGTCTGCTGGCGA
ACGCCACCGGCGCCGGATTCCTCGGAGGGATCATCGCCGGTTTTGCCGCCGGGTATTTTATGCTGCTGATCCGTCATCTG
CTCGACGGTCTGCCGCGCCAGTACGAAGGGCTGAAGTCGATCTTTATTATGCCGCTGATAGGCGTCCTGGTGATCGGCGT
GATGATGGTCCTGCTCGGCCAGCCGGTGGCAGCGATCAACAATGGCATGATGAACTGGCTCAGCTCTTTACAAGAAGCCA
ACCCAATCCTGCTGGGGATCGTGGTAGGCGCAATGTGCTCCTTCGACTTCGGCGGGCCGGTCAACAAAGCGGCCTATGTC
ACCGGTACGTTGCTGCTGGGTCAAGGTAACTACTTCTTTATGGCCGGTGTATCTGCCGCCTGCATCACGCCGCCGCTGGT
CATCGCTCTGGCGACCACGTTCTTTCCGAAAGGCTTTAGCGAGGAGGAACGCGCCGCCGGGATGGTCAACTATATCCTCG
GCTGCACGCACATTACCGAAGGTGCAATTCCCTTTGCCGCCAAGGATCCGCTGCGGGTGATCCCGATGATGATGATCGCC
TCCTCAATTTCTGCGGTACTCAGCTACAGCCTGCATATTCAGGTTCCGGCACCGCACGGCGGCTTCTTAATCCTGCCGCT
GGTCAGTAAACCGTTGATGTGGGTGCTGTGCATTCTCGCCGGTTCCGCCTGCGGGGCACTTATGCTGGGATGCTGGCGGC
TGTGGCAAAAACGCACTGACAAAGCGACAGCTCTTGCTGGCGCCATGAAATAA

Upstream 100 bases:

>100_bases
ACCGGCGCTGATGTGGCCGCCTCCGCCGGGCTGGGTAAATTAAACCGTACGCAAGAATTATTACAACAACTTCAGATCGT
CCAATTATAAAGGTCCGATT

Downstream 100 bases:

>100_bases
GGAGCAAGCTATGCAACTGACTTCTGCCGATATTTGTTTCGATCGTCAGGTCAAAACGAAAGACGAGGCGCTACAGCGTG
TCGTTGCAGCCCTGACCCAT

Product: fructose-specific PTS family enzyme IIB component

Products: NA

Alternate protein names: EIIABC-Fru; Fructose-specific phosphotransferase enzyme IIA component; EII-Fru; PTS system fructose-specific EIIA component; Fructose-specific phosphotransferase enzyme IIB component; EIII-Fru; PTS system fructose-specific EIIB component; Fructose permease IIC component; PTS system fructose-specific EIIC component [H]

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MKKIIAVTGCPTGIAHTFMAEEALKTAAKKLNVEIKVETNGASGIENAITPEDLKDIYGVIIAADKDVNAERFNGLPVIE
VPVKEAIHHPAELINKVVNGQAPRRQGFSAATDPDEKYERESFGRQIYKHLMSGVSNMLPFVVAGGILIAVSFLWGIYSA
DPNSPQYNVIAATLMKVGQQAFSIMVPVFTAYIAWSISGRPGMVAGFVGGLLANATGAGFLGGIIAGFAAGYFMLLIRHL
LDGLPRQYEGLKSIFIMPLIGVLVIGVMMVLLGQPVAAINNGMMNWLSSLQEANPILLGIVVGAMCSFDFGGPVNKAAYV
TGTLLLGQGNYFFMAGVSAACITPPLVIALATTFFPKGFSEEERAAGMVNYILGCTHITEGAIPFAAKDPLRVIPMMMIA
SSISAVLSYSLHIQVPAPHGGFLILPLVSKPLMWVLCILAGSACGALMLGCWRLWQKRTDKATALAGAMK

Sequences:

>Translated_470_residues
MKKIIAVTGCPTGIAHTFMAEEALKTAAKKLNVEIKVETNGASGIENAITPEDLKDIYGVIIAADKDVNAERFNGLPVIE
VPVKEAIHHPAELINKVVNGQAPRRQGFSAATDPDEKYERESFGRQIYKHLMSGVSNMLPFVVAGGILIAVSFLWGIYSA
DPNSPQYNVIAATLMKVGQQAFSIMVPVFTAYIAWSISGRPGMVAGFVGGLLANATGAGFLGGIIAGFAAGYFMLLIRHL
LDGLPRQYEGLKSIFIMPLIGVLVIGVMMVLLGQPVAAINNGMMNWLSSLQEANPILLGIVVGAMCSFDFGGPVNKAAYV
TGTLLLGQGNYFFMAGVSAACITPPLVIALATTFFPKGFSEEERAAGMVNYILGCTHITEGAIPFAAKDPLRVIPMMMIA
SSISAVLSYSLHIQVPAPHGGFLILPLVSKPLMWVLCILAGSACGALMLGCWRLWQKRTDKATALAGAMK
>Mature_470_residues
MKKIIAVTGCPTGIAHTFMAEEALKTAAKKLNVEIKVETNGASGIENAITPEDLKDIYGVIIAADKDVNAERFNGLPVIE
VPVKEAIHHPAELINKVVNGQAPRRQGFSAATDPDEKYERESFGRQIYKHLMSGVSNMLPFVVAGGILIAVSFLWGIYSA
DPNSPQYNVIAATLMKVGQQAFSIMVPVFTAYIAWSISGRPGMVAGFVGGLLANATGAGFLGGIIAGFAAGYFMLLIRHL
LDGLPRQYEGLKSIFIMPLIGVLVIGVMMVLLGQPVAAINNGMMNWLSSLQEANPILLGIVVGAMCSFDFGGPVNKAAYV
TGTLLLGQGNYFFMAGVSAACITPPLVIALATTFFPKGFSEEERAAGMVNYILGCTHITEGAIPFAAKDPLRVIPMMMIA
SSISAVLSYSLHIQVPAPHGGFLILPLVSKPLMWVLCILAGSACGALMLGCWRLWQKRTDKATALAGAMK

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1299

COG function: function code G; Phosphotransferase system, fructose-specific IIC component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1788492, Length=462, Percent_Identity=43.7229437229437, Blast_Score=347, Evalue=1e-96,
Organism=Escherichia coli, GI1786951, Length=462, Percent_Identity=35.0649350649351, Blast_Score=259, Evalue=2e-70,
Organism=Escherichia coli, GI1790386, Length=320, Percent_Identity=39.6875, Blast_Score=214, Evalue=9e-57,
Organism=Escherichia coli, GI87082348, Length=479, Percent_Identity=31.7327766179541, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI1788729, Length=396, Percent_Identity=26.2626262626263, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1790387, Length=98, Percent_Identity=43.8775510204082, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1788730, Length=107, Percent_Identity=39.2523364485981, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR013011
- InterPro:   IPR003501
- InterPro:   IPR003352
- InterPro:   IPR013014
- InterPro:   IPR004715
- InterPro:   IPR003353
- InterPro:   IPR006327 [H]

Pfam domain/function: PF00359 PTS_EIIA_2; PF02378 PTS_EIIC; PF02302 PTS_IIB [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 49907; Mature: 49907

Theoretical pI: Translated: 8.27; Mature: 8.27

Prosite motif: PS51099 PTS_EIIB_TYPE_2 ; PS51104 PTS_EIIC_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIIAVTGCPTGIAHTFMAEEALKTAAKKLNVEIKVETNGASGIENAITPEDLKDIYGV
CCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCHHHHHHHHHE
IIAADKDVNAERFNGLPVIEVPVKEAIHHPAELINKVVNGQAPRRQGFSAATDPDEKYER
EEECCCCCCHHHHCCCCEEEECHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHH
ESFGRQIYKHLMSGVSNMLPFVVAGGILIAVSFLWGIYSADPNSPQYNVIAATLMKVGQQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
AFSIMVPVFTAYIAWSISGRPGMVAGFVGGLLANATGAGFLGGIIAGFAAGYFMLLIRHL
HHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
LDGLPRQYEGLKSIFIMPLIGVLVIGVMMVLLGQPVAAINNGMMNWLSSLQEANPILLGI
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHH
VVGAMCSFDFGGPVNKAAYVTGTLLLGQGNYFFMAGVSAACITPPLVIALATTFFPKGFS
HHHHHHCCCCCCCCCCCCEEEEEEEEECCCEEEEECCCHHHHCHHHHHHHHHHHCCCCCC
EEERAAGMVNYILGCTHITEGAIPFAAKDPLRVIPMMMIASSISAVLSYSLHIQVPAPHG
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEEECCCCC
GFLILPLVSKPLMWVLCILAGSACGALMLGCWRLWQKRTDKATALAGAMK
CEEEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHCCC
>Mature Secondary Structure
MKKIIAVTGCPTGIAHTFMAEEALKTAAKKLNVEIKVETNGASGIENAITPEDLKDIYGV
CCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCHHHHHHHHHE
IIAADKDVNAERFNGLPVIEVPVKEAIHHPAELINKVVNGQAPRRQGFSAATDPDEKYER
EEECCCCCCHHHHCCCCEEEECHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHH
ESFGRQIYKHLMSGVSNMLPFVVAGGILIAVSFLWGIYSADPNSPQYNVIAATLMKVGQQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
AFSIMVPVFTAYIAWSISGRPGMVAGFVGGLLANATGAGFLGGIIAGFAAGYFMLLIRHL
HHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
LDGLPRQYEGLKSIFIMPLIGVLVIGVMMVLLGQPVAAINNGMMNWLSSLQEANPILLGI
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHH
VVGAMCSFDFGGPVNKAAYVTGTLLLGQGNYFFMAGVSAACITPPLVIALATTFFPKGFS
HHHHHHCCCCCCCCCCCCEEEEEEEEECCCEEEEECCCHHHHCHHHHHHHHHHHCCCCCC
EEERAAGMVNYILGCTHITEGAIPFAAKDPLRVIPMMMIASSISAVLSYSLHIQVPAPHG
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEEECCCCC
GFLILPLVSKPLMWVLCILAGSACGALMLGCWRLWQKRTDKATALAGAMK
CEEEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]