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The map label for this gene is pfkB [H]

Identifier: 238896643

GI number: 238896643

Start: 4622620

End: 4623534

Strand: Direct

Name: pfkB [H]

Synonym: KP1_4848

Alternate gene names: 238896643

Gene position: 4622620-4623534 (Clockwise)

Preceding gene: 238896642

Following gene: 238896644

Centisome position: 88.07

GC content: 53.55

Gene sequence:

>915_bases
GTGATCCACACCCTGACACTGAATACCGCGATCGATATGAATATCTTTTGCGATCCGCTCCAGCCGGCGGCAGTCAACCG
CACCCGGCAAACGGAATATTGCCCGAATGGTAAAGGGGTTAACGTCTCGCTGGTATTACATCATTACCAGCAGCCAACCC
ATGTCATGGGCATATTCGGCGGTTTTACCGGCCGCTATATCGTTGAGGAACTACGCAAACAGCATATTAATGTCACACCG
GCATGGGTCGCTGAACCGACCCGTATAAATATATTTATTAACGACGGCAGTGATGAATACAAACTGGTAAATCCCGGCGC
GCGGATTGACGATGAAGGAAAAGAACAAATTCTTCACCATTTACAGTGCGTCGCGCCCGGTGATTATTTAGTTATCAGCG
GCAGTCTGCCACCGGGAATAGAGAGCCGGTTCTATAGCGAGATTCTGGTGCTGTGCCAGCAGAAAGGGTGTGAAGTCATC
TTAGATATCAGCCACCCGGTGCTGCGCCAGCTGTTAGAGTGGCGTCCACTATTGATTAAACCCAATGACGACGAGCTGAA
AGAGATCTTTGGTCTGGATGTCAGCAGCCCCCGGCGGGTACGCGAAGCAATGCAAACGCTCCACCAGCTCGGGGCTCGCA
ATGTTCTGCTGACCCTTGGCGCGCAAGGGCTCTATTTTTCGAACGGCGCGCAACTGTGGTTCTGCTCGGCACCGAAAATC
GAACTGGTCAGCTCGGCCTGCGCCGGCGATGCCGCGCTGGGTGCATTCCTCAGCCATTGGCTTAACACAGACGATATTCC
CCAGGCCCTGGCGCTGGCCAGCGCCACCGGCGCTGATGTGGCCGCCTCCGCCGGGCTGGGTAAATTAAACCGTACGCAAG
AATTATTACAACAACTTCAGATCGTCCAATTATAA

Upstream 100 bases:

>100_bases
GAAACCGGCGAAAGCGGCAATGAAAGAGGTCGTTCGCAAAGTCATTCACGTTTGTGGCTGCGAAGGACAACTGTAATCAG
CGGATCGAAGGAGCCTTGCT

Downstream 100 bases:

>100_bases
AGGTCCGATTATGAAAAAAATTATTGCCGTGACCGGCTGCCCTACAGGTATTGCTCATACATTTATGGCTGAAGAGGCCT
TAAAAACCGCAGCGAAAAAA

Product: 6-phosphofructokinase II

Products: NA

Alternate protein names: Fructose 1-phosphate kinase [H]

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MIHTLTLNTAIDMNIFCDPLQPAAVNRTRQTEYCPNGKGVNVSLVLHHYQQPTHVMGIFGGFTGRYIVEELRKQHINVTP
AWVAEPTRINIFINDGSDEYKLVNPGARIDDEGKEQILHHLQCVAPGDYLVISGSLPPGIESRFYSEILVLCQQKGCEVI
LDISHPVLRQLLEWRPLLIKPNDDELKEIFGLDVSSPRRVREAMQTLHQLGARNVLLTLGAQGLYFSNGAQLWFCSAPKI
ELVSSACAGDAALGAFLSHWLNTDDIPQALALASATGADVAASAGLGKLNRTQELLQQLQIVQL

Sequences:

>Translated_304_residues
MIHTLTLNTAIDMNIFCDPLQPAAVNRTRQTEYCPNGKGVNVSLVLHHYQQPTHVMGIFGGFTGRYIVEELRKQHINVTP
AWVAEPTRINIFINDGSDEYKLVNPGARIDDEGKEQILHHLQCVAPGDYLVISGSLPPGIESRFYSEILVLCQQKGCEVI
LDISHPVLRQLLEWRPLLIKPNDDELKEIFGLDVSSPRRVREAMQTLHQLGARNVLLTLGAQGLYFSNGAQLWFCSAPKI
ELVSSACAGDAALGAFLSHWLNTDDIPQALALASATGADVAASAGLGKLNRTQELLQQLQIVQL
>Mature_304_residues
MIHTLTLNTAIDMNIFCDPLQPAAVNRTRQTEYCPNGKGVNVSLVLHHYQQPTHVMGIFGGFTGRYIVEELRKQHINVTP
AWVAEPTRINIFINDGSDEYKLVNPGARIDDEGKEQILHHLQCVAPGDYLVISGSLPPGIESRFYSEILVLCQQKGCEVI
LDISHPVLRQLLEWRPLLIKPNDDELKEIFGLDVSSPRRVREAMQTLHQLGARNVLLTLGAQGLYFSNGAQLWFCSAPKI
ELVSSACAGDAALGAFLSHWLNTDDIPQALALASATGADVAASAGLGKLNRTQELLQQLQIVQL

Specific function: Key control step of glycolysis. [C]

COG id: COG1105

COG function: function code G; Fructose-1-phosphate kinase and related fructose-6-phosphate kinase (PfkB)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carbohydrate kinase pfkB family [H]

Homologues:

Organism=Escherichia coli, GI1788017, Length=281, Percent_Identity=26.6903914590747, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1788493, Length=294, Percent_Identity=27.2108843537415, Blast_Score=98, Evalue=5e-22,

Paralogues:

None

Copy number: 496 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022463
- InterPro:   IPR011611
- InterPro:   IPR002173
- InterPro:   IPR017583 [H]

Pfam domain/function: PF00294 PfkB [H]

EC number: =2.7.1.56 [H]

Molecular weight: Translated: 33478; Mature: 33478

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIHTLTLNTAIDMNIFCDPLQPAAVNRTRQTEYCPNGKGVNVSLVLHHYQQPTHVMGIFG
CEEEEEECCEEECEEEECCCCCHHCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEHHHC
GFTGRYIVEELRKQHINVTPAWVAEPTRINIFINDGSDEYKLVNPGARIDDEGKEQILHH
CCCHHHHHHHHHHHHCCCCHHHCCCCEEEEEEEECCCCCEEEECCCCCCCCCHHHHHHHH
LQCVAPGDYLVISGSLPPGIESRFYSEILVLCQQKGCEVILDISHPVLRQLLEWRPLLIK
HHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHCCCCEEEC
PNDDELKEIFGLDVSSPRRVREAMQTLHQLGARNVLLTLGAQGLYFSNGAQLWFCSAPKI
CCHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCEEEECCCEEEEECCCCH
ELVSSACAGDAALGAFLSHWLNTDDIPQALALASATGADVAASAGLGKLNRTQELLQQLQ
HHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHCCCCCCHHHHHHHHHHH
IVQL
HHCC
>Mature Secondary Structure
MIHTLTLNTAIDMNIFCDPLQPAAVNRTRQTEYCPNGKGVNVSLVLHHYQQPTHVMGIFG
CEEEEEECCEEECEEEECCCCCHHCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEHHHC
GFTGRYIVEELRKQHINVTPAWVAEPTRINIFINDGSDEYKLVNPGARIDDEGKEQILHH
CCCHHHHHHHHHHHHCCCCHHHCCCCEEEEEEEECCCCCEEEECCCCCCCCCHHHHHHHH
LQCVAPGDYLVISGSLPPGIESRFYSEILVLCQQKGCEVILDISHPVLRQLLEWRPLLIK
HHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHCCCCEEEC
PNDDELKEIFGLDVSSPRRVREAMQTLHQLGARNVLLTLGAQGLYFSNGAQLWFCSAPKI
CCHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCEEEECCCEEEEECCCCH
ELVSSACAGDAALGAFLSHWLNTDDIPQALALASATGADVAASAGLGKLNRTQELLQQLQ
HHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHCCCCCCHHHHHHHHHHH
IVQL
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]