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The map label for this gene is lacY [H]

Identifier: 238896370

GI number: 238896370

Start: 4352891

End: 4354132

Strand: Reverse

Name: lacY [H]

Synonym: KP1_4537

Alternate gene names: 238896370

Gene position: 4354132-4352891 (Counterclockwise)

Preceding gene: 238896371

Following gene: 238896369

Centisome position: 82.96

GC content: 57.81

Gene sequence:

>1242_bases
ATGATGAAAGCGCCTCACTCACACAGCTACCCGTTGCTCAGTGCCTTACTGTTTTTCTTTTTCGTGACCTGGTCGTCATC
CGGCTCCTTGCTCTCTATCTGGCTTCACCAGGAGGTTGGGCTGAAGGCAGGCGATACCGGTATTATCTATGCCGTACTGT
CGGTTTCCGCGCTGTTTGCGCAAGTTTGCTATGGATTTATCCAGGACAAGCTGGGGCTGCGCAAACATCTGCTCTGGTAT
ATCACCGCGCTGCTGATCCTCTCTGGTCCGGCGTATCTGCTGTTCGGCCACCTGCTGAAAATCAACGTCCTGTTGGGCAG
CATCTTTGGCGGGATCTATATCGGTTTGACCTTTAACGGCGGCATCGGCGTGCTGGAATCTTACACCGAGCGCGTGGCGC
GTCAGAGCCAGTTTGAATTCGGCAGGGCGCGGATGTGGGGCTCGCTGGGCTGGGCGGTGGCCACCTTCTTCGCCGGCCTG
CTGTTTAACATCAATCCGCAGCTAAACTTCCTCGTCGCCAGCTGTTCCGGGCTGGTGTTCTTTATCCTGTTGGCGCGGCT
GCGGGTCTCCTCGGCGCCGCATGCAATGCAGGAGGCGGTATCGGGCGGTAAGGTGACCCTCGAAGATGCGCTGCGCCTGC
TGACGCTGCCGCGCTTTTGGGCGCTGGTCTTCTTCGTCATCGGCACCTGCATTTACGGCGTCTATGACCAGCAGTTCCCG
GTCTATTTCTCATCGCAGTTCGCGACCCTGCAGGAAGGCAATGAGATGTATGGCTACCTCAACTCCTTCCAGGTGTTCCT
CGAGGCGGCCGGCATGTTCTGCGCGCCGTGGCTGGTGAACCGCATCGGCGCCAAAAACGGTCTGATTTTCGCCGGCATGG
TGATGGCGATGCGCATGGTGGCCTCCGGTCTGGTGGAGGGGCCGCTGCTGATCTCCATCACCAAACTGCTGCACGCGGTG
GAGTTGCCGATACTGCTGGTGGCTATCTTTAAGTACAACAGCCTCAACTTCGATAAACGTCTCTCCTCCACCCTCTATCT
GGTGGGCTTCGCCTGCACCAGCTCGATTATCGCCAGCGTATTGTCGCCGCTGGCGGGCTACAGCTATGAAAAATATGGCT
TCGCCCAGTCCTACCTGATCATGGGCCTGCTGGTGTTCTGCACCACCTTTATTTCCATCTTCTTGTTGCGCTCCGGCAAA
TCCTCCGCCGATCCGCTGGTGTCGCAACCTACCGCTATCTGA

Upstream 100 bases:

>100_bases
GTTAACTTTTGTGATTAACATCATATATCGCAGCGAATCCACCTGCGTTTTGTTTTTTGTTAACGTTACCATAATTGCAT
TCCTCTAACACGAGAATGTC

Downstream 100 bases:

>100_bases
ATCAAAAAGAGTGAAAATGATGACTTACACAATCTCCCGGGCTGAACAGGTACTCCAGACACAACGCCAGGCGCTGAATC
TGCGCTGGTATCCGCACTAT

Product: galactoside permease

Products: Proton [Cytoplasm]; lactose [Cytoplasm] [C]

Alternate protein names: Sucrose permease [H]

Number of amino acids: Translated: 413; Mature: 413

Protein sequence:

>413_residues
MMKAPHSHSYPLLSALLFFFFVTWSSSGSLLSIWLHQEVGLKAGDTGIIYAVLSVSALFAQVCYGFIQDKLGLRKHLLWY
ITALLILSGPAYLLFGHLLKINVLLGSIFGGIYIGLTFNGGIGVLESYTERVARQSQFEFGRARMWGSLGWAVATFFAGL
LFNINPQLNFLVASCSGLVFFILLARLRVSSAPHAMQEAVSGGKVTLEDALRLLTLPRFWALVFFVIGTCIYGVYDQQFP
VYFSSQFATLQEGNEMYGYLNSFQVFLEAAGMFCAPWLVNRIGAKNGLIFAGMVMAMRMVASGLVEGPLLISITKLLHAV
ELPILLVAIFKYNSLNFDKRLSSTLYLVGFACTSSIIASVLSPLAGYSYEKYGFAQSYLIMGLLVFCTTFISIFLLRSGK
SSADPLVSQPTAI

Sequences:

>Translated_413_residues
MMKAPHSHSYPLLSALLFFFFVTWSSSGSLLSIWLHQEVGLKAGDTGIIYAVLSVSALFAQVCYGFIQDKLGLRKHLLWY
ITALLILSGPAYLLFGHLLKINVLLGSIFGGIYIGLTFNGGIGVLESYTERVARQSQFEFGRARMWGSLGWAVATFFAGL
LFNINPQLNFLVASCSGLVFFILLARLRVSSAPHAMQEAVSGGKVTLEDALRLLTLPRFWALVFFVIGTCIYGVYDQQFP
VYFSSQFATLQEGNEMYGYLNSFQVFLEAAGMFCAPWLVNRIGAKNGLIFAGMVMAMRMVASGLVEGPLLISITKLLHAV
ELPILLVAIFKYNSLNFDKRLSSTLYLVGFACTSSIIASVLSPLAGYSYEKYGFAQSYLIMGLLVFCTTFISIFLLRSGK
SSADPLVSQPTAI
>Mature_413_residues
MMKAPHSHSYPLLSALLFFFFVTWSSSGSLLSIWLHQEVGLKAGDTGIIYAVLSVSALFAQVCYGFIQDKLGLRKHLLWY
ITALLILSGPAYLLFGHLLKINVLLGSIFGGIYIGLTFNGGIGVLESYTERVARQSQFEFGRARMWGSLGWAVATFFAGL
LFNINPQLNFLVASCSGLVFFILLARLRVSSAPHAMQEAVSGGKVTLEDALRLLTLPRFWALVFFVIGTCIYGVYDQQFP
VYFSSQFATLQEGNEMYGYLNSFQVFLEAAGMFCAPWLVNRIGAKNGLIFAGMVMAMRMVASGLVEGPLLISITKLLHAV
ELPILLVAIFKYNSLNFDKRLSSTLYLVGFACTSSIIASVLSPLAGYSYEKYGFAQSYLIMGLLVFCTTFISIFLLRSGK
SSADPLVSQPTAI

Specific function: Proton symport transport system. Has an essential role in sucrose metabolism [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lacY/rafB permease family [H]

Homologues:

Organism=Escherichia coli, GI1786538, Length=369, Percent_Identity=42.5474254742547, Blast_Score=287, Evalue=9e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022814
- InterPro:   IPR000576
- InterPro:   IPR018457
- InterPro:   IPR020846
- InterPro:   IPR016196 [H]

Pfam domain/function: PF01306 LacY_symp [H]

EC number: NA

Molecular weight: Translated: 45374; Mature: 45374

Theoretical pI: Translated: 9.04; Mature: 9.04

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKAPHSHSYPLLSALLFFFFVTWSSSGSLLSIWLHQEVGLKAGDTGIIYAVLSVSALFA
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEHHCCCCCCCCHHHHHHHHHHHHHH
QVCYGFIQDKLGLRKHLLWYITALLILSGPAYLLFGHLLKINVLLGSIFGGIYIGLTFNG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHEEEEEEECC
GIGVLESYTERVARQSQFEFGRARMWGSLGWAVATFFAGLLFNINPQLNFLVASCSGLVF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
FILLARLRVSSAPHAMQEAVSGGKVTLEDALRLLTLPRFWALVFFVIGTCIYGVYDQQFP
HHHHHHHHHCCCCHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
VYFSSQFATLQEGNEMYGYLNSFQVFLEAAGMFCAPWLVNRIGAKNGLIFAGMVMAMRMV
EEECCHHHHHHCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
ASGLVEGPLLISITKLLHAVELPILLVAIFKYNSLNFDKRLSSTLYLVGFACTSSIIASV
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LSPLAGYSYEKYGFAQSYLIMGLLVFCTTFISIFLLRSGKSSADPLVSQPTAI
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MMKAPHSHSYPLLSALLFFFFVTWSSSGSLLSIWLHQEVGLKAGDTGIIYAVLSVSALFA
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEHHCCCCCCCCHHHHHHHHHHHHHH
QVCYGFIQDKLGLRKHLLWYITALLILSGPAYLLFGHLLKINVLLGSIFGGIYIGLTFNG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHEEEEEEECC
GIGVLESYTERVARQSQFEFGRARMWGSLGWAVATFFAGLLFNINPQLNFLVASCSGLVF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
FILLARLRVSSAPHAMQEAVSGGKVTLEDALRLLTLPRFWALVFFVIGTCIYGVYDQQFP
HHHHHHHHHCCCCHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
VYFSSQFATLQEGNEMYGYLNSFQVFLEAAGMFCAPWLVNRIGAKNGLIFAGMVMAMRMV
EEECCHHHHHHCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
ASGLVEGPLLISITKLLHAVELPILLVAIFKYNSLNFDKRLSSTLYLVGFACTSSIIASV
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LSPLAGYSYEKYGFAQSYLIMGLLVFCTTFISIFLLRSGKSSADPLVSQPTAI
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; lactose [Periplasm] [C]

Specific reaction: Proton [Periplasm] + lactose [Periplasm] = Proton [Cytoplasm] + lactose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1435727 [H]