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The map label for this gene is cscA [H]

Identifier: 238896369

GI number: 238896369

Start: 4351438

End: 4352880

Strand: Reverse

Name: cscA [H]

Synonym: KP1_4536

Alternate gene names: 238896369

Gene position: 4352880-4351438 (Counterclockwise)

Preceding gene: 238896370

Following gene: 238896368

Centisome position: 82.94

GC content: 62.37

Gene sequence:

>1443_bases
GTGAAAATGATGACTTACACAATCTCCCGGGCTGAACAGGTACTCCAGACACAACGCCAGGCGCTGAATCTGCGCTGGTA
TCCGCACTATCACCTGGCGGCCCGCGCCGGGTGGATCAACGACCCAAACGGTCTGGTGTGGTTCGACGGCTGGTACCATG
CCTTTTATCAACATCACCCGTACTCCACCCAGTGGGGACCGATGCACTGGGGGCACGCGCGCAGTAAGGATCTGGTGCAC
TGGGAGCATTTGCCGGTAGCCCTGGCGCCGGAGGGCCCGGAGGATAAAGACGGCTGCTTCTCCGGCTCGGCGGTGGTCGA
TGGCGATACCCTGGCGCTGATTTACACCGGGCATAAGTTCCACGGCGATCCTGGCGACGAAGCCAATCTGTATCAGGTGC
AGTGTCTGGCCACCAGCCGCGACGGGATCCACTTTGAGCGCCAGGGAATGGTGGTCGACACCCCGCCGGGTATGCACCAC
TTCCGTGACCCGAAGGTCTGGCGTGAAGGAGACAGCTGGTACATGATCGTCGGCGCGCGCGAAGGCGATACCGGCCAGGT
GCGGCTGTACCGTTCAGCCGATCTGCGCCAGTGGCAGGACGCGGGGGTGCTGGATGAAGCGGAAAGCACCATGGGCTATA
TGTGGGAGTGCCCGGACTTCTTCACCCTCAACGGCAAACGCGTTCTGATGTTTTCCCCACAGGGGATGCAGGCCGCGGGG
TTCAGCAACCGCAATCTGTTCCAGAGCGGCTATCTGATCGGCGAGTGGCAGCCAGGCCAGCGTTTTATTCGCCATGGCGA
ATTCCGGGAGATGGATAACGGCCACGATTTCTATGCCCCGCAAAGCTTCGCCACCCCCGATGGCCGGCGGATCGTGATTG
GCTGGCTGGATATGTGGGAATCGCCGCTGCCGGAGCAGCAGGACGGCTGGGCGGGCATGCTCTCCCTGCCGCGCGAGCTG
AGCCTGAGTGCGGATGACCGCCTGCAGATGCGCCCGGCGAAAGAAGTGGAGAGCCTGCGCGGCGCCTGGTTCCCGTGGCC
GGTCAGCACCCTGAACAACCAGCAGACGACGATGGTCGACAACTGCGAGGCGATGGAGGTGAATCTGCGCTGGGACTGCG
CCCGCAGCAGCGCCGAGCAGTATGGTCTGCGCTTTGGCGATGGCCTGCGCATCTACGTGGATGCCCAACAGCAGCGTCTG
GTGCTGGAGCGGCATTATCCGCAGTATGGCCTGTGCGGGACGCGCAGCGTGCCGTTGACCGCCGGAGCGGATCTCAACCT
GCGAATATTTTTCGATAGCTCTTCGGTGGAGGTTTTTGTCAATGACGGCGAAGCCTGCCTGAGCAGCCGTATCTATCCGC
AGGCTCCCCGCCGTGAATTAGCGTTATTTGCCTGGAGCGGCTCGGCGGCATTAAACGAAGCCGGCGCCTGGCAGCTCGAA
TAA

Upstream 100 bases:

>100_bases
GTGTTCTGCACCACCTTTATTTCCATCTTCTTGTTGCGCTCCGGCAAATCCTCCGCCGATCCGCTGGTGTCGCAACCTAC
CGCTATCTGAATCAAAAAGA

Downstream 100 bases:

>100_bases
TAATAAGGCTGTTGTCGTTAATAACCCGTCTTTGACGATGGGCGGGTTATTATTGCCTGCATCGTCGTGCGTTACCCATT
AATAAAAAAATTCAGTTCAT

Product: putative family 32 glycoside hydrolase

Products: NA

Alternate protein names: Sucrase; Invertase [H]

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MKMMTYTISRAEQVLQTQRQALNLRWYPHYHLAARAGWINDPNGLVWFDGWYHAFYQHHPYSTQWGPMHWGHARSKDLVH
WEHLPVALAPEGPEDKDGCFSGSAVVDGDTLALIYTGHKFHGDPGDEANLYQVQCLATSRDGIHFERQGMVVDTPPGMHH
FRDPKVWREGDSWYMIVGAREGDTGQVRLYRSADLRQWQDAGVLDEAESTMGYMWECPDFFTLNGKRVLMFSPQGMQAAG
FSNRNLFQSGYLIGEWQPGQRFIRHGEFREMDNGHDFYAPQSFATPDGRRIVIGWLDMWESPLPEQQDGWAGMLSLPREL
SLSADDRLQMRPAKEVESLRGAWFPWPVSTLNNQQTTMVDNCEAMEVNLRWDCARSSAEQYGLRFGDGLRIYVDAQQQRL
VLERHYPQYGLCGTRSVPLTAGADLNLRIFFDSSSVEVFVNDGEACLSSRIYPQAPRRELALFAWSGSAALNEAGAWQLE

Sequences:

>Translated_480_residues
MKMMTYTISRAEQVLQTQRQALNLRWYPHYHLAARAGWINDPNGLVWFDGWYHAFYQHHPYSTQWGPMHWGHARSKDLVH
WEHLPVALAPEGPEDKDGCFSGSAVVDGDTLALIYTGHKFHGDPGDEANLYQVQCLATSRDGIHFERQGMVVDTPPGMHH
FRDPKVWREGDSWYMIVGAREGDTGQVRLYRSADLRQWQDAGVLDEAESTMGYMWECPDFFTLNGKRVLMFSPQGMQAAG
FSNRNLFQSGYLIGEWQPGQRFIRHGEFREMDNGHDFYAPQSFATPDGRRIVIGWLDMWESPLPEQQDGWAGMLSLPREL
SLSADDRLQMRPAKEVESLRGAWFPWPVSTLNNQQTTMVDNCEAMEVNLRWDCARSSAEQYGLRFGDGLRIYVDAQQQRL
VLERHYPQYGLCGTRSVPLTAGADLNLRIFFDSSSVEVFVNDGEACLSSRIYPQAPRRELALFAWSGSAALNEAGAWQLE
>Mature_480_residues
MKMMTYTISRAEQVLQTQRQALNLRWYPHYHLAARAGWINDPNGLVWFDGWYHAFYQHHPYSTQWGPMHWGHARSKDLVH
WEHLPVALAPEGPEDKDGCFSGSAVVDGDTLALIYTGHKFHGDPGDEANLYQVQCLATSRDGIHFERQGMVVDTPPGMHH
FRDPKVWREGDSWYMIVGAREGDTGQVRLYRSADLRQWQDAGVLDEAESTMGYMWECPDFFTLNGKRVLMFSPQGMQAAG
FSNRNLFQSGYLIGEWQPGQRFIRHGEFREMDNGHDFYAPQSFATPDGRRIVIGWLDMWESPLPEQQDGWAGMLSLPREL
SLSADDRLQMRPAKEVESLRGAWFPWPVSTLNNQQTTMVDNCEAMEVNLRWDCARSSAEQYGLRFGDGLRIYVDAQQQRL
VLERHYPQYGLCGTRSVPLTAGADLNLRIFFDSSSVEVFVNDGEACLSSRIYPQAPRRELALFAWSGSAALNEAGAWQLE

Specific function: Enables the bacterium to metabolize sucrose as a sole carbon source [H]

COG id: COG1621

COG function: function code G; Beta-fructosidases (levanase/invertase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 32 family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6322029, Length=348, Percent_Identity=29.5977011494253, Blast_Score=126, Evalue=9e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008985
- InterPro:   IPR001362
- InterPro:   IPR018053
- InterPro:   IPR013189
- InterPro:   IPR013148
- InterPro:   IPR006232 [H]

Pfam domain/function: PF08244 Glyco_hydro_32C; PF00251 Glyco_hydro_32N [H]

EC number: =3.2.1.26 [H]

Molecular weight: Translated: 54855; Mature: 54855

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMMTYTISRAEQVLQTQRQALNLRWYPHYHLAARAGWINDPNGLVWFDGWYHAFYQHHP
CCEEEEHHHHHHHHHHHHHHHEEEEECCCEEEEEECCCCCCCCCEEEECCCEEEHHHCCC
YSTQWGPMHWGHARSKDLVHWEHLPVALAPEGPEDKDGCFSGSAVVDGDTLALIYTGHKF
CCCCCCCCCCCCCCCCCCEEEECCCEEECCCCCCCCCCCCCCCEEECCCEEEEEEECCEE
HGDPGDEANLYQVQCLATSRDGIHFERQGMVVDTPPGMHHFRDPKVWREGDSWYMIVGAR
CCCCCCCCCEEEEEEEEECCCCCEEEECCEEEECCCCCCCCCCCCCEECCCCEEEEEEEC
EGDTGQVRLYRSADLRQWQDAGVLDEAESTMGYMWECPDFFTLNGKRVLMFSPQGMQAAG
CCCCCEEEEEECCCCHHHHCCCCCCCCHHCCCEEEECCCEEEECCCEEEEECCCCCEECC
FSNRNLFQSGYLIGEWQPGQRFIRHGEFREMDNGHDFYAPQSFATPDGRRIVIGWLDMWE
CCCCCEECCCCEEEECCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEHHHCC
SPLPEQQDGWAGMLSLPRELSLSADDRLQMRPAKEVESLRGAWFPWPVSTLNNQQTTMVD
CCCCCCCCCCCHHHCCCHHCCCCCCCCEECCCHHHHHHHCCCCCCCCHHHCCCCCEEEEC
NCEAMEVNLRWDCARSSAEQYGLRFGDGLRIYVDAQQQRLVLERHYPQYGLCGTRSVPLT
CCEEEEEEEEEECCCCCHHHCCCEECCCEEEEEECCCHHHHHHHCCCCCCCCCCCCCCEE
AGADLNLRIFFDSSSVEVFVNDGEACLSSRIYPQAPRRELALFAWSGSAALNEAGAWQLE
CCCCEEEEEEEECCCEEEEEECCHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCCEECC
>Mature Secondary Structure
MKMMTYTISRAEQVLQTQRQALNLRWYPHYHLAARAGWINDPNGLVWFDGWYHAFYQHHP
CCEEEEHHHHHHHHHHHHHHHEEEEECCCEEEEEECCCCCCCCCEEEECCCEEEHHHCCC
YSTQWGPMHWGHARSKDLVHWEHLPVALAPEGPEDKDGCFSGSAVVDGDTLALIYTGHKF
CCCCCCCCCCCCCCCCCCEEEECCCEEECCCCCCCCCCCCCCCEEECCCEEEEEEECCEE
HGDPGDEANLYQVQCLATSRDGIHFERQGMVVDTPPGMHHFRDPKVWREGDSWYMIVGAR
CCCCCCCCCEEEEEEEEECCCCCEEEECCEEEECCCCCCCCCCCCCEECCCCEEEEEEEC
EGDTGQVRLYRSADLRQWQDAGVLDEAESTMGYMWECPDFFTLNGKRVLMFSPQGMQAAG
CCCCCEEEEEECCCCHHHHCCCCCCCCHHCCCEEEECCCEEEECCCEEEEECCCCCEECC
FSNRNLFQSGYLIGEWQPGQRFIRHGEFREMDNGHDFYAPQSFATPDGRRIVIGWLDMWE
CCCCCEECCCCEEEECCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEHHHCC
SPLPEQQDGWAGMLSLPRELSLSADDRLQMRPAKEVESLRGAWFPWPVSTLNNQQTTMVD
CCCCCCCCCCCHHHCCCHHCCCCCCCCEECCCHHHHHHHCCCCCCCCHHHCCCCCEEEEC
NCEAMEVNLRWDCARSSAEQYGLRFGDGLRIYVDAQQQRLVLERHYPQYGLCGTRSVPLT
CCEEEEEEEEEECCCCCHHHCCCEECCCEEEEEECCCHHHHHHHCCCCCCCCCCCCCCEE
AGADLNLRIFFDSSSVEVFVNDGEACLSSRIYPQAPRRELALFAWSGSAALNEAGAWQLE
CCCCEEEEEEEECCCEEEEEECCHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12218016 [H]