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The map label for this gene is pduQ [H]

Identifier: 238896323

GI number: 238896323

Start: 4298589

End: 4299701

Strand: Direct

Name: pduQ [H]

Synonym: KP1_4486

Alternate gene names: 238896323

Gene position: 4298589-4299701 (Clockwise)

Preceding gene: 238896322

Following gene: 238896324

Centisome position: 81.9

GC content: 64.96

Gene sequence:

>1113_bases
ATGCATACCTTTTCTCTGCAAACGCGCCTCTACAGCGGCCCGGGCAGCCTGGCCGCGCTGCAGCGCTTTAGCCATCAGCA
CATCTGGATCGTCTGCGACGGCTTCCTGGCGCGCTCGCCGCTGCTTGACCGACTGCGCGCCGCGCTGCCCGCCAGCAACC
GCGTCAGCGTGTTCAGCGATATTACACCGGATCCGACCATTCACACCGTGGCGAAAGGGATAGCGCAGATGCAGGCCCTG
CGTCCGCAGGTGGTGATCGGCTTCGGCGGCGGCTCGGCGATGGATGCCGCCAAGGCTATCGTCTGGTTCAGCCAGCAGGG
CGGTCTGCCTGTCGACACCTGCGTGGCGATCCCCACCACCAGCGGTACCGGTTCGGAAGTGACCAGCGCCTGCGTCATCA
GCGACCCGGAAAAAGGGATCAAGTACCCGCTGTTCCATGAGGCGCTCTGTCCCGACATGGCGATCATCGACCCGACGCTG
GTGGTTAGCGTACCGCCCACCATCACAGCCCATACCGGGCTGGACGCGCTGACCCACGCCCTGGAGGCATGGGTCTCGCC
GCAGGCCACCGATTTTACCGATGCGCTGGCGGAAAAGGCCGCCAGGCTGGTGTTTCGCGCCCTGCCCGTTGCGATTCGTC
AGGGCGACTGCATTGCGACCCGCAGCAAAATGCACAATGCATCAACCCTCGCCGGTATGGCCTTTAGCCAGGCTGGCCTT
GGGCTCAATCATGCGATCGCCCATCAGCTTGGCGGCCAGTTTCACCTCCCCCATGGCCTGGCCAATGCGCTGCTGCTGAC
CGCGGTGATCCGCTTCAATGCCGGCGAGCCGCGAGCGGCTAAGCGCTATGCACGCCTGGCCAGGGCCTGCCGCTTCTGCC
CGCCCGAAGCTGGCGAACAGGAGGCTTTCCAGGCGCTGCTTACCGCGGTGGAAACGCTGAAACAGCAGTGCGCCATTCCC
ACCCTTAAGGGCGCGCTGCAGGAAAAGTATCCCCTTTTCTTATCACGAATTCCCGCCATGGTGCCGGCCGCGCTGGCCGA
CGCCACCCTGCGCACCAACCCGCGTCCGGTCGATGGCGCGGCCATCGCGCAACTGCTGGAGAACCTGCAATGA

Upstream 100 bases:

>100_bases
CCACGCCAACCGGAGAAGGCACCACCTCCGCGCGGACGTTCGCCCGCCTGCGGCGCTGCGTGTTGACCAACGGTTTTTCC
ATTCGCTAACCGGGGGCGCC

Downstream 100 bases:

>100_bases
GTGAAGCGATAGTGCCACTATCAAGCATTGATGCGACGGAGATCCGCGAACGCGTTCGGGCCGCTGGCGTCGTAGGAGCC
GGTGGCGCAGGCTTTCCGAC

Product: propanol dehydrogenase

Products: NA

Alternate protein names: Alcohol dehydrogenase; ADH; Acetaldehyde dehydrogenase [acetylating]; ACDH; Pyruvate-formate-lyase deactivase; PFL deactivase [H]

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MHTFSLQTRLYSGPGSLAALQRFSHQHIWIVCDGFLARSPLLDRLRAALPASNRVSVFSDITPDPTIHTVAKGIAQMQAL
RPQVVIGFGGGSAMDAAKAIVWFSQQGGLPVDTCVAIPTTSGTGSEVTSACVISDPEKGIKYPLFHEALCPDMAIIDPTL
VVSVPPTITAHTGLDALTHALEAWVSPQATDFTDALAEKAARLVFRALPVAIRQGDCIATRSKMHNASTLAGMAFSQAGL
GLNHAIAHQLGGQFHLPHGLANALLLTAVIRFNAGEPRAAKRYARLARACRFCPPEAGEQEAFQALLTAVETLKQQCAIP
TLKGALQEKYPLFLSRIPAMVPAALADATLRTNPRPVDGAAIAQLLENLQ

Sequences:

>Translated_370_residues
MHTFSLQTRLYSGPGSLAALQRFSHQHIWIVCDGFLARSPLLDRLRAALPASNRVSVFSDITPDPTIHTVAKGIAQMQAL
RPQVVIGFGGGSAMDAAKAIVWFSQQGGLPVDTCVAIPTTSGTGSEVTSACVISDPEKGIKYPLFHEALCPDMAIIDPTL
VVSVPPTITAHTGLDALTHALEAWVSPQATDFTDALAEKAARLVFRALPVAIRQGDCIATRSKMHNASTLAGMAFSQAGL
GLNHAIAHQLGGQFHLPHGLANALLLTAVIRFNAGEPRAAKRYARLARACRFCPPEAGEQEAFQALLTAVETLKQQCAIP
TLKGALQEKYPLFLSRIPAMVPAALADATLRTNPRPVDGAAIAQLLENLQ
>Mature_370_residues
MHTFSLQTRLYSGPGSLAALQRFSHQHIWIVCDGFLARSPLLDRLRAALPASNRVSVFSDITPDPTIHTVAKGIAQMQAL
RPQVVIGFGGGSAMDAAKAIVWFSQQGGLPVDTCVAIPTTSGTGSEVTSACVISDPEKGIKYPLFHEALCPDMAIIDPTL
VVSVPPTITAHTGLDALTHALEAWVSPQATDFTDALAEKAARLVFRALPVAIRQGDCIATRSKMHNASTLAGMAFSQAGL
GLNHAIAHQLGGQFHLPHGLANALLLTAVIRFNAGEPRAAKRYARLARACRFCPPEAGEQEAFQALLTAVETLKQQCAIP
TLKGALQEKYPLFLSRIPAMVPAALADATLRTNPRPVDGAAIAQLLENLQ

Specific function: This enzyme has three activities:ADH, ACDH, and PFL- deactivase. In aerobic conditions it acts as a hydrogen peroxide scavenger. The PFL deactivase activity catalyzes the quenching of the pyruvate-formate-lyase catalyst in an iron, NAD, and CoA dependent

COG id: COG1454

COG function: function code C; Alcohol dehydrogenase, class IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the iron- containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI133922590, Length=310, Percent_Identity=26.1290322580645, Blast_Score=81, Evalue=1e-15,
Organism=Escherichia coli, GI1787493, Length=392, Percent_Identity=36.9897959183673, Blast_Score=225, Evalue=4e-60,
Organism=Escherichia coli, GI87082107, Length=375, Percent_Identity=37.6, Blast_Score=200, Evalue=1e-52,
Organism=Escherichia coli, GI48994951, Length=351, Percent_Identity=36.1823361823362, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI1789163, Length=370, Percent_Identity=33.7837837837838, Blast_Score=162, Evalue=3e-41,
Organism=Escherichia coli, GI1789386, Length=363, Percent_Identity=26.4462809917355, Blast_Score=96, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17537053, Length=266, Percent_Identity=27.4436090225564, Blast_Score=101, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6321181, Length=364, Percent_Identity=34.8901098901099, Blast_Score=184, Evalue=3e-47,
Organism=Drosophila melanogaster, GI24657991, Length=417, Percent_Identity=25.8992805755396, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211
- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR012079 [H]

Pfam domain/function: PF00171 Aldedh; PF00465 Fe-ADH [H]

EC number: =1.1.1.1; =1.2.1.10 [H]

Molecular weight: Translated: 39310; Mature: 39310

Theoretical pI: Translated: 8.24; Mature: 8.24

Prosite motif: PS00913 ADH_IRON_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHTFSLQTRLYSGPGSLAALQRFSHQHIWIVCDGFLARSPLLDRLRAALPASNRVSVFSD
CCCEEEEHEEECCCCHHHHHHHCCCCEEEEEECCHHHCCHHHHHHHHHCCCCCCEEEEEC
ITPDPTIHTVAKGIAQMQALRPQVVIGFGGGSAMDAAKAIVWFSQQGGLPVDTCVAIPTT
CCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHEEEECCCCCCHHHEEEEECC
SGTGSEVTSACVISDPEKGIKYPLFHEALCPDMAIIDPTLVVSVPPTITAHTGLDALTHA
CCCCHHHHHEEEECCCHHCCCCCHHHHHHCCCCEEECCEEEEECCCCEEECCCHHHHHHH
LEAWVSPQATDFTDALAEKAARLVFRALPVAIRQGDCIATRSKMHNASTLAGMAFSQAGL
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHCCC
GLNHAIAHQLGGQFHLPHGLANALLLTAVIRFNAGEPRAAKRYARLARACRFCPPEAGEQ
CHHHHHHHHCCCCEECCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCHH
EAFQALLTAVETLKQQCAIPTLKGALQEKYPLFLSRIPAMVPAALADATLRTNPRPVDGA
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
AIAQLLENLQ
HHHHHHHHCC
>Mature Secondary Structure
MHTFSLQTRLYSGPGSLAALQRFSHQHIWIVCDGFLARSPLLDRLRAALPASNRVSVFSD
CCCEEEEHEEECCCCHHHHHHHCCCCEEEEEECCHHHCCHHHHHHHHHCCCCCCEEEEEC
ITPDPTIHTVAKGIAQMQALRPQVVIGFGGGSAMDAAKAIVWFSQQGGLPVDTCVAIPTT
CCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHEEEECCCCCCHHHEEEEECC
SGTGSEVTSACVISDPEKGIKYPLFHEALCPDMAIIDPTLVVSVPPTITAHTGLDALTHA
CCCCHHHHHEEEECCCHHCCCCCHHHHHHCCCCEEECCEEEEECCCCEEECCCHHHHHHH
LEAWVSPQATDFTDALAEKAARLVFRALPVAIRQGDCIATRSKMHNASTLAGMAFSQAGL
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHCCC
GLNHAIAHQLGGQFHLPHGLANALLLTAVIRFNAGEPRAAKRYARLARACRFCPPEAGEQ
CHHHHHHHHCCCCEECCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCHH
EAFQALLTAVETLKQQCAIPTLKGALQEKYPLFLSRIPAMVPAALADATLRTNPRPVDGA
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
AIAQLLENLQ
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]