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The map label for this gene is cbiJ [H]

Identifier: 238896295

GI number: 238896295

Start: 4276220

End: 4277005

Strand: Reverse

Name: cbiJ [H]

Synonym: KP1_4456

Alternate gene names: 238896295

Gene position: 4277005-4276220 (Counterclockwise)

Preceding gene: 238896296

Following gene: 238896294

Centisome position: 81.49

GC content: 65.65

Gene sequence:

>786_bases
GTGAATCACGGCGACGTGCTGGTTATCGGGGGCACCAGCGATGCGCGGGCGATCTGCCAGCAGCTGGACGCCGCCGACGT
GCGCTACACCCTGTCTGTGGCGACCCCTACCGGCGAGCGGCTGGCCGGCGATATCCGCGGGCAGATACGCTGCGGGCGAA
TGGAGTGGCAGCAGATGGCGGAGTGGCTCAGAGCGCAGCGCACCCGCTGGGTGATCGATGCCTCTCACCCCTACGCCGAG
GTGGTCAGCCAGAATATTGTGCGCGCCTGCGCTAGCGTCGGGGTTCTGCTCAGCCGCTATCAAAGGCCGGAGCAGCTGAG
CGATCTGCGCCATCCGCTACTTCACGTGGTCGGCGATCTGAACGAGGCCTGCGCGGTGGCTCGTCGCTTCGGCGAGCGGA
TCCTGCTGACCACCGGCAGCAAAGATCTGGCGGCCTGGCGGGCGGGACTGGCGGAGAAAACGCTGCTCGCCCGGGTGCTG
CCGGTGCCGGAGGTGGTTCAGCACTGTAGCGATCTGGGGTTTGGCGTCGGGGAGATCTTTGCCCTCTGCGGGCCGTTCAG
CGCCGAATTTAACGCCGCCTTTTATCGCCAGTGCCGGGCCGATGTTGTGGTGACGAAAGCCTCGGGGGCGGAAGGAGGCT
ATCAGGAAAAAGTACAACCCTGTCTTGATGCGGGGATCCCCTGCATCGTCATTACCCGTCCGGCGCCGCTGGTGACGGGC
GATGAACTACTGCAAAGCCAGGCCGATTTCACTGCGCGGCTGACGCGCTGGCTGTCGGCGACTTGA

Upstream 100 bases:

>100_bases
TGGATTTTGCCCCAGTGGATATGACCAGTCTGGTGATCGTTGGCAACAAAGCGACTTACATCGACAACGGCCTGATGATC
ACCCCGCGAGGCTACGCGCT

Downstream 100 bases:

>100_bases
GGAAGAGGATATGAAAAAAGCACTTCTGGTGGTGAGCTTTGGCACCAGCTATCACGACACCCGCGAAAAGAACATCGACG
CCTGCGAGCGCGATCTGGCC

Product: cobalt-precorrin-6x reductase

Products: Precorrin 6A; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MNHGDVLVIGGTSDARAICQQLDAADVRYTLSVATPTGERLAGDIRGQIRCGRMEWQQMAEWLRAQRTRWVIDASHPYAE
VVSQNIVRACASVGVLLSRYQRPEQLSDLRHPLLHVVGDLNEACAVARRFGERILLTTGSKDLAAWRAGLAEKTLLARVL
PVPEVVQHCSDLGFGVGEIFALCGPFSAEFNAAFYRQCRADVVVTKASGAEGGYQEKVQPCLDAGIPCIVITRPAPLVTG
DELLQSQADFTARLTRWLSAT

Sequences:

>Translated_261_residues
MNHGDVLVIGGTSDARAICQQLDAADVRYTLSVATPTGERLAGDIRGQIRCGRMEWQQMAEWLRAQRTRWVIDASHPYAE
VVSQNIVRACASVGVLLSRYQRPEQLSDLRHPLLHVVGDLNEACAVARRFGERILLTTGSKDLAAWRAGLAEKTLLARVL
PVPEVVQHCSDLGFGVGEIFALCGPFSAEFNAAFYRQCRADVVVTKASGAEGGYQEKVQPCLDAGIPCIVITRPAPLVTG
DELLQSQADFTARLTRWLSAT
>Mature_261_residues
MNHGDVLVIGGTSDARAICQQLDAADVRYTLSVATPTGERLAGDIRGQIRCGRMEWQQMAEWLRAQRTRWVIDASHPYAE
VVSQNIVRACASVGVLLSRYQRPEQLSDLRHPLLHVVGDLNEACAVARRFGERILLTTGSKDLAAWRAGLAEKTLLARVL
PVPEVVQHCSDLGFGVGEIFALCGPFSAEFNAAFYRQCRADVVVTKASGAEGGYQEKVQPCLDAGIPCIVITRPAPLVTG
DELLQSQADFTARLTRWLSAT

Specific function: Catalyzes the reduction of the macrocycle of cobalt- precorrin-6X into cobalt-precorrin-6Y [H]

COG id: COG2099

COG function: function code H; Precorrin-6x reductase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin-6x reductase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003723 [H]

Pfam domain/function: PF02571 CbiJ [H]

EC number: 1.3.1.54

Molecular weight: Translated: 28473; Mature: 28473

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: PS51014 COBK_CBIJ

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHGDVLVIGGTSDARAICQQLDAADVRYTLSVATPTGERLAGDIRGQIRCGRMEWQQMA
CCCCCEEEEECCCHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHCCEEECHHHHHHHH
EWLRAQRTRWVIDASHPYAEVVSQNIVRACASVGVLLSRYQRPEQLSDLRHPLLHVVGDL
HHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCH
NEACAVARRFGERILLTTGSKDLAAWRAGLAEKTLLARVLPVPEVVQHCSDLGFGVGEIF
HHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHH
ALCGPFSAEFNAAFYRQCRADVVVTKASGAEGGYQEKVQPCLDAGIPCIVITRPAPLVTG
HHHCCCCCHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCH
DELLQSQADFTARLTRWLSAT
HHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNHGDVLVIGGTSDARAICQQLDAADVRYTLSVATPTGERLAGDIRGQIRCGRMEWQQMA
CCCCCEEEEECCCHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHCCEEECHHHHHHHH
EWLRAQRTRWVIDASHPYAEVVSQNIVRACASVGVLLSRYQRPEQLSDLRHPLLHVVGDL
HHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCH
NEACAVARRFGERILLTTGSKDLAAWRAGLAEKTLLARVLPVPEVVQHCSDLGFGVGEIF
HHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHH
ALCGPFSAEFNAAFYRQCRADVVVTKASGAEGGYQEKVQPCLDAGIPCIVITRPAPLVTG
HHHCCCCCHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCH
DELLQSQADFTARLTRWLSAT
HHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Precorrin 6B; NADP+

Specific reaction: precorrin-6B + NADP+ = precorrin-6A + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501034; 11677609 [H]