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The map label for this gene is gcvA [H]

Identifier: 238896280

GI number: 238896280

Start: 4263055

End: 4263972

Strand: Reverse

Name: gcvA [H]

Synonym: KP1_4439

Alternate gene names: 238896280

Gene position: 4263972-4263055 (Counterclockwise)

Preceding gene: 238896281

Following gene: 238896279

Centisome position: 81.24

GC content: 53.38

Gene sequence:

>918_bases
ATGTCGAAGCGATTACCACCTCTGAATGCATTACGTGTTTTTGATGCAGCCGCACGTCATCTCAGCTTCACTCGGGCAGC
CGATGAGCTTTTTGTGACGCAGGCCGCAGTCAGCCATCAAATCAAGTCTCTCGAGGATTTTCTGGGCCTTAAGCTGTTCC
GTCGGCGCAATCGTTCGCTGCTGTTAACGGAAGAAGGGCAGAGCTATTTTCAGGATATTAAAGAGATTTTTTCGCAACTG
ACGGAAGCGACGCGTAAACTGCAGGCGCGCAGCGCCAAAGGGGCATTAACGGTCAGTTTATTGCCAAGTTTCGCCATTCA
GTGGCTGGTGCCAAGACTCACAAGCTTTAACTCAGCTTATCCGGGAATCGACGTCAGGATCCAGGCGGTGGACCGTCAGG
AGGATAAGCTGGCGGACGATGTTGATGTGGCGATTTTTTACGGCCGCGGCAACTGGCCGGGCCTGCGTGTGGAAAAATTG
TACGCCGAGTATCTGCTGCCGGTCTGTTCCCCGCTCTTGCTCACCGGCGACAAAGCATTGAAAACGCCTGCCGATCTGGC
GCAACATACCCTGCTTCACGATGCGTCCCGCCGCGACTGGCAAACTTATACCCGTCAGCTTGGTCTAAGCCATATTAATG
TGCAGCAGGGGCCGATTTTCAGCCATAGCGCGATGGTGCTGCAGGCGGCCATTCACGGGCAGGGCGTGGCGCTGGCCAAC
AACGTCATGGCGCAGTCCGAAATTGAAGCCGGTCGTCTGGTGTGTCCGTTTAATGATGTTCTGGTCAGTAAGAATGCGTT
TTACCTTGTTTGCCATGACAGTCAGGCAGAACTGGGTAAAATAGCCGCTTTCCGGCAGTGGATCCTGGCGAAAGCGGCAA
GTGAACAAGAAAAATTCCGCTTTCGTTATGAACAATAA

Upstream 100 bases:

>100_bases
CCTCAACGGACAATTTATAATGGCTCAGATAAAAAAAACTAATAGGTTAATCATTGTTTCCTATTTGTTAAATTGAGTTA
ACATCTAAGCCAGAATAACC

Downstream 100 bases:

>100_bases
TTACTTGTAGGGTAACAACATGACCAGCCGTTTTATGCTGATATTCGCCGCGATTAGCGGCTTTATCTTTGTGGCTCTGG
GCGCCTTTGGCGCGCATGTC

Product: DNA-binding transcriptional activator GcvA

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 305; Mature: 304

Protein sequence:

>305_residues
MSKRLPPLNALRVFDAAARHLSFTRAADELFVTQAAVSHQIKSLEDFLGLKLFRRRNRSLLLTEEGQSYFQDIKEIFSQL
TEATRKLQARSAKGALTVSLLPSFAIQWLVPRLTSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
YAEYLLPVCSPLLLTGDKALKTPADLAQHTLLHDASRRDWQTYTRQLGLSHINVQQGPIFSHSAMVLQAAIHGQGVALAN
NVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQAELGKIAAFRQWILAKAASEQEKFRFRYEQ

Sequences:

>Translated_305_residues
MSKRLPPLNALRVFDAAARHLSFTRAADELFVTQAAVSHQIKSLEDFLGLKLFRRRNRSLLLTEEGQSYFQDIKEIFSQL
TEATRKLQARSAKGALTVSLLPSFAIQWLVPRLTSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
YAEYLLPVCSPLLLTGDKALKTPADLAQHTLLHDASRRDWQTYTRQLGLSHINVQQGPIFSHSAMVLQAAIHGQGVALAN
NVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQAELGKIAAFRQWILAKAASEQEKFRFRYEQ
>Mature_304_residues
SKRLPPLNALRVFDAAARHLSFTRAADELFVTQAAVSHQIKSLEDFLGLKLFRRRNRSLLLTEEGQSYFQDIKEIFSQLT
EATRKLQARSAKGALTVSLLPSFAIQWLVPRLTSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKLY
AEYLLPVCSPLLLTGDKALKTPADLAQHTLLHDASRRDWQTYTRQLGLSHINVQQGPIFSHSAMVLQAAIHGQGVALANN
VMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQAELGKIAAFRQWILAKAASEQEKFRFRYEQ

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=305, Percent_Identity=96.7213114754098, Blast_Score=609, Evalue=1e-176,
Organism=Escherichia coli, GI1786448, Length=286, Percent_Identity=34.6153846153846, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI1788706, Length=288, Percent_Identity=30.5555555555556, Blast_Score=138, Evalue=5e-34,
Organism=Escherichia coli, GI145693193, Length=296, Percent_Identity=27.7027027027027, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1786401, Length=284, Percent_Identity=28.5211267605634, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI157672245, Length=157, Percent_Identity=34.3949044585987, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI87081978, Length=264, Percent_Identity=31.8181818181818, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1789639, Length=250, Percent_Identity=27.2, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1787128, Length=305, Percent_Identity=23.2786885245902, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1788887, Length=176, Percent_Identity=34.0909090909091, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI145693105, Length=143, Percent_Identity=29.3706293706294, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789440, Length=263, Percent_Identity=26.615969581749, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1790262, Length=198, Percent_Identity=27.7777777777778, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1790783, Length=262, Percent_Identity=29.3893129770992, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1787879, Length=130, Percent_Identity=31.5384615384615, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34299; Mature: 34167

Theoretical pI: Translated: 9.31; Mature: 9.31

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKRLPPLNALRVFDAAARHLSFTRAADELFVTQAAVSHQIKSLEDFLGLKLFRRRNRSL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
LLTEEGQSYFQDIKEIFSQLTEATRKLQARSAKGALTVSLLPSFAIQWLVPRLTSFNSAY
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHCCCCC
PGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKLYAEYLLPVCSPLLLTGDKAL
CCCEEEEEEECCCHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHH
KTPADLAQHTLLHDASRRDWQTYTRQLGLSHINVQQGPIFSHSAMVLQAAIHGQGVALAN
CCHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHCCCCHHHHH
NVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQAELGKIAAFRQWILAKAASEQEKFR
HHHHHHHCCCCCEECCCHHHEECCCEEEEEEECCHHHHHHHHHHHHHHHHHHHCHHHHHH
FRYEQ
CCCCC
>Mature Secondary Structure 
SKRLPPLNALRVFDAAARHLSFTRAADELFVTQAAVSHQIKSLEDFLGLKLFRRRNRSL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
LLTEEGQSYFQDIKEIFSQLTEATRKLQARSAKGALTVSLLPSFAIQWLVPRLTSFNSAY
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHCCCCC
PGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKLYAEYLLPVCSPLLLTGDKAL
CCCEEEEEEECCCHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHH
KTPADLAQHTLLHDASRRDWQTYTRQLGLSHINVQQGPIFSHSAMVLQAAIHGQGVALAN
CCHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHCCCCHHHHH
NVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQAELGKIAAFRQWILAKAASEQEKFR
HHHHHHHCCCCCEECCCHHHEECCCEEEEEEECCHHHHHHHHHHHHHHHHHHHCHHHHHH
FRYEQ
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]