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The map label for this gene is pcm [H]

Identifier: 238896223

GI number: 238896223

Start: 4196916

End: 4197542

Strand: Reverse

Name: pcm [H]

Synonym: KP1_4375

Alternate gene names: 238896223

Gene position: 4197542-4196916 (Counterclockwise)

Preceding gene: 238896224

Following gene: 238896222

Centisome position: 79.98

GC content: 58.37

Gene sequence:

>627_bases
ATGGTAAGTAAACGTGTTGAAAGTTTGTTGAATCAACTGCGCACGCAGGGCATCGTCGATGAACGTGTCCTCGAGGCGAT
CGCGCTGGTCCCGCGCGAAAAGTTTGTTGATGAAGCCTTTGAACATAAAGCGTGGGAAAACACCGCGCTGCCGATTGGCC
AGGGGCAGACCATCTCCCAGCCGTATATGGTGGCGCGGATGACCGAGCTGCTGACGCTGACGCCGGAGTCGCGGGTGCTG
GAGATCGGCACCGGTTCCGGCTACCAGACGGCCATCCTGGCGCATCTGGTGCATCACGTATGCTCGGTAGAGCGAATTAA
GAGTTTACAGTGGCAGGCGCGTCGTCGCCTGAAACAACTTGATTTACATAATGTTTCAACCCGTCATGGCGATGGGTGGC
AAGGTTGGCAGGCGCGGGCGCCGTTTGATGCCATTATTGTGACGGCGGCGCCGCCGGAAATACCGACCGCGCTGCTGGCG
CAGCTGGATGATGACGGCGTGCTGGTGCTGCCGGTCGGCGAAGAGCATCAGTTTCTCAAACGGATCCGTCGTCGGGGCAA
CGAATTTATCATTGATACCGTCGAGGCCGTTCGCTTTGTTCCCCTGGTGAAGGGGGAGCTGGCCTGA

Upstream 100 bases:

>100_bases
AAGGCTATGTTTCCGTGACGCCATTGCACGTCGATTTAACCGCCCATCAGGCGCATGAGGTGGTCACCGATTGGCTGGAA
CGTGTCGGGGTTGACAGGCA

Downstream 100 bases:

>100_bases
GACCCGGATTATTCCAGGTTTTTTTTGCCTGACTTTAGGCGTAGGGTGGACACATTTTTCAGTGTCCTGGCCGGGCGCCG
CCCGGTAAGCAAGATATTGG

Product: protein-L-isoaspartate O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT [H]

Number of amino acids: Translated: 208; Mature: 208

Protein sequence:

>208_residues
MVSKRVESLLNQLRTQGIVDERVLEAIALVPREKFVDEAFEHKAWENTALPIGQGQTISQPYMVARMTELLTLTPESRVL
EIGTGSGYQTAILAHLVHHVCSVERIKSLQWQARRRLKQLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALLA
QLDDDGVLVLPVGEEHQFLKRIRRRGNEFIIDTVEAVRFVPLVKGELA

Sequences:

>Translated_208_residues
MVSKRVESLLNQLRTQGIVDERVLEAIALVPREKFVDEAFEHKAWENTALPIGQGQTISQPYMVARMTELLTLTPESRVL
EIGTGSGYQTAILAHLVHHVCSVERIKSLQWQARRRLKQLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALLA
QLDDDGVLVLPVGEEHQFLKRIRRRGNEFIIDTVEAVRFVPLVKGELA
>Mature_208_residues
MVSKRVESLLNQLRTQGIVDERVLEAIALVPREKFVDEAFEHKAWENTALPIGQGQTISQPYMVARMTELLTLTPESRVL
EIGTGSGYQTAILAHLVHHVCSVERIKSLQWQARRRLKQLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALLA
QLDDDGVLVLPVGEEHQFLKRIRRRGNEFIIDTVEAVRFVPLVKGELA

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins [H]

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI226530908, Length=212, Percent_Identity=31.6037735849057, Blast_Score=80, Evalue=8e-16,
Organism=Escherichia coli, GI1789100, Length=208, Percent_Identity=87.5, Blast_Score=379, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI71983477, Length=211, Percent_Identity=30.3317535545024, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI193207222, Length=210, Percent_Identity=29.0476190476191, Blast_Score=68, Evalue=3e-12,
Organism=Drosophila melanogaster, GI17981723, Length=220, Percent_Identity=31.8181818181818, Blast_Score=93, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000682 [H]

Pfam domain/function: PF01135 PCMT [H]

EC number: =2.1.1.77 [H]

Molecular weight: Translated: 23406; Mature: 23406

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: PS01279 PCMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSKRVESLLNQLRTQGIVDERVLEAIALVPREKFVDEAFEHKAWENTALPIGQGQTISQ
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
PYMVARMTELLTLTPESRVLEIGTGSGYQTAILAHLVHHVCSVERIKSLQWQARRRLKQL
HHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALLAQLDDDGVLVLPVGEEHQFLK
HHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHCCCCCEEEEECCCHHHHHH
RIRRRGNEFIIDTVEAVRFVPLVKGELA
HHHHCCCEEEEHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MVSKRVESLLNQLRTQGIVDERVLEAIALVPREKFVDEAFEHKAWENTALPIGQGQTISQ
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
PYMVARMTELLTLTPESRVLEIGTGSGYQTAILAHLVHHVCSVERIKSLQWQARRRLKQL
HHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALLAQLDDDGVLVLPVGEEHQFLK
HHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHCCCCCEEEEECCCHHHHHH
RIRRRGNEFIIDTVEAVRFVPLVKGELA
HHHHCCCEEEEHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA