Click here to switch to the map view.

The map label for this gene is rpoS [H]

Identifier: 238896221

GI number: 238896221

Start: 4194510

End: 4195610

Strand: Reverse

Name: rpoS [H]

Synonym: KP1_4373

Alternate gene names: 238896221

Gene position: 4195610-4194510 (Counterclockwise)

Preceding gene: 238896222

Following gene: 238896218

Centisome position: 79.94

GC content: 54.77

Gene sequence:

>1101_bases
GTGTCTACGTTCCTCTCGCAGAGAGGGCAGGGGAAATCGTCCAGGCGTCGTTTGTTGATGTGCCGTGGAAGGTGCTTTGC
TCAGGGATCACGGGTAGGAGCCACCTTTATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGACGCGGAATTTG
ATGAGAACGGAATTGAGGTTTTCGACGAGAAAGCCTTAGTAGAAGAGGAACCCAGTGATAGCGATCTGGCTGAGGAAGAG
CTGCTGTCGCAAGGCGCAACCCAGCGCGTACTTGACGCCACTCAGCTTTATCTTGGAGAGATTGGTTATTCCCCACTGCT
GACCGCGGAAGAGGAAGTCTATTTCGCGCGTCGCGCACTGCGTGGTGATGTCGCTTCACGCCGTCGCATGATTGAAAGTA
ACTTGCGTCTGGTGGTGAAGATTGCCCGTCGTTACAGCAATCGTGGTCTGGCGCTGCTGGATCTGATTGAAGAAGGTAAC
CTCGGCCTGATCCGCGCCGTTGAGAAGTTTGACCCGGAACGTGGGTTCCGTTTTTCGACCTACGCGACCTGGTGGATCCG
CCAGACTATTGAACGGGCGATCATGAACCAAACCCGTACGATCCGTTTGCCGATCCATATTGTTAAAGAGCTGAACGTCT
ATCTGCGTACCGCGCGCGAGTTGTCCCATAAGCTGGACCACGAGCCTAGCGCGGAAGAGATCGCCGAACAGCTGGACAAA
CCGGTCGATGACGTCAGCCGTATGCTGCGTCTGAACGAGCGCATCACCTCCGTGGATACTCCGCTGGGTGGCGATTCAGA
AAAAGCGCTGCTGGATATTCTGGCCGATGAGAAAGAGAACGGCCCGGAAGACACCACGCAGGACGATGATATGAAGCAAA
GTATCGTCAAATGGCTGTTCGAACTGAACGCCAAGCAGCGTGAAGTTCTGGCACGTCGTTTTGGTCTGCTGGGTTATGAA
GCTGCCACGCTGGAGGATGTGGGCCGTGAAATTGGCCTGACCCGCGAACGCGTGCGGCAGATCCAGGTGGAAGGTCTGCG
TCGCCTGCGGGAAATTCTGCAGGGACAGGGGCTGAATATCGAAGCGCTCTTCCGCGAATAA

Upstream 100 bases:

>100_bases
CGTTACAAGGGGAAATCCGTCAACCCGCTGCAGTACTTACCGCAGCGATAATTTGGCAAAGTACGGATAAGCGAAGCATC
AGCTAACAGGCGCCAGGGCG

Downstream 100 bases:

>100_bases
GACGTTTTCTGTCAAAAAGGCCCCCTCGAAAGTCGGGGCCTTTTTCTTTGGGCTAGCCGAGGCGGGTTTCCTGCAGATGG
CGCCAGACAATTTTCCCTTC

Product: RNA polymerase sigma factor RpoS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 366; Mature: 365

Protein sequence:

>366_residues
MSTFLSQRGQGKSSRRRLLMCRGRCFAQGSRVGATFMSQNTLKVHDLNEDAEFDENGIEVFDEKALVEEEPSDSDLAEEE
LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLVVKIARRYSNRGLALLDLIEEGN
LGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMNQTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDK
PVDDVSRMLRLNERITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYE
AATLEDVGREIGLTRERVRQIQVEGLRRLREILQGQGLNIEALFRE

Sequences:

>Translated_366_residues
MSTFLSQRGQGKSSRRRLLMCRGRCFAQGSRVGATFMSQNTLKVHDLNEDAEFDENGIEVFDEKALVEEEPSDSDLAEEE
LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLVVKIARRYSNRGLALLDLIEEGN
LGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMNQTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDK
PVDDVSRMLRLNERITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYE
AATLEDVGREIGLTRERVRQIQVEGLRRLREILQGQGLNIEALFRE
>Mature_365_residues
STFLSQRGQGKSSRRRLLMCRGRCFAQGSRVGATFMSQNTLKVHDLNEDAEFDENGIEVFDEKALVEEEPSDSDLAEEEL
LSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLVVKIARRYSNRGLALLDLIEEGNL
GLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMNQTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKP
VDDVSRMLRLNERITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEA
ATLEDVGREIGLTRERVRQIQVEGLRRLREILQGQGLNIEALFRE

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is required for the normal expression of the enterotoxin, yst, in the stationary phase [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789098, Length=330, Percent_Identity=98.4848484848485, Blast_Score=651, Evalue=0.0,
Organism=Escherichia coli, GI1789448, Length=247, Percent_Identity=42.1052631578947, Blast_Score=210, Evalue=1e-55,
Organism=Escherichia coli, GI1789871, Length=268, Percent_Identity=26.865671641791, Blast_Score=92, Evalue=5e-20,

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012761
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 41966; Mature: 41835

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTFLSQRGQGKSSRRRLLMCRGRCFAQGSRVGATFMSQNTLKVHDLNEDAEFDENGIEV
CCCHHHHCCCCHHHHHHHHHHHCCHHHCCCCCCHHHHCCCCEEEEECCCCCCCCCCCCHH
FDEKALVEEEPSDSDLAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRAL
HHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
RGDVASRRRMIESNLRLVVKIARRYSNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFST
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHCCCCHHHHHHHHCCCCCCCEEHH
YATWWIRQTIERAIMNQTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDK
HHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCC
PVDDVSRMLRLNERITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLF
CHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHH
ELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQGQGLNI
HCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCE
EALFRE
EEECCC
>Mature Secondary Structure 
STFLSQRGQGKSSRRRLLMCRGRCFAQGSRVGATFMSQNTLKVHDLNEDAEFDENGIEV
CCHHHHCCCCHHHHHHHHHHHCCHHHCCCCCCHHHHCCCCEEEEECCCCCCCCCCCCHH
FDEKALVEEEPSDSDLAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRAL
HHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
RGDVASRRRMIESNLRLVVKIARRYSNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFST
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHCCCCHHHHHHHHCCCCCCCEEHH
YATWWIRQTIERAIMNQTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDK
HHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCC
PVDDVSRMLRLNERITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLF
CHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHH
ELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQGQGLNI
HCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCE
EALFRE
EEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7729893 [H]