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The map label for this gene is ilvI [H]

Identifier: 238896205

GI number: 238896205

Start: 4177397

End: 4179106

Strand: Direct

Name: ilvI [H]

Synonym: KP1_4354

Alternate gene names: 238896205

Gene position: 4177397-4179106 (Clockwise)

Preceding gene: 238896204

Following gene: 238896207

Centisome position: 79.59

GC content: 64.27

Gene sequence:

>1710_bases
ATGGCCTCTATCACATTATCGGCTGCTGAACTGTTACTGCATCGGCTACAGGCGCTGGACGTGGCGTATATTTTTATCAA
CTCGGGCACCGATTATCCTCCAGTCATTGAAGCCTGGGCAAAGGCCCGGGCGACCGGGCAAAAGGTACCGGAGTTAGTGA
TCTGCCCGCATGAAAACGCCGCCATCGGCATGGCCCATGGCTATTACCTCGGCACCGGCAAGGTGCAGGCGGTGATGGTT
CACACCAACGTCGGGCTAGCCAACGCCGCCTGCGGCGTCATCAATCTGGCGAACAGCAATATTCCGGTGCTGATTTTTGG
CGGCCGCACGCCGATAAGCGAACATTCCCACTTCGGCTGCCGTAATACGCCGATTGGCTACGGCCAGGAGATGCGCGACC
AGGCGGCGCTGATCCGTGAATCCGTGAAGTGGGATTTTGAACTGCGGCTGGCCGATCAGATCGGCGAGCATGTTGACCGG
GCCTGGGCGATCGCTAGCTCCCTGCCGAAAGGGCCGGTCTACCTGAGCTTACCGCGTGAGCCACTGTGCGAAACCTTTGC
CGTCGATGAGGCCGCGCTGCAGGCTGGCCCTTCGCAGCAGCCGGTCCGCTATGCGCCAGTCCGGGAAGATATCGCCCGTG
CAGCCGAGGCAATCGCCTGCGCCAGACACCCGGTCATCTTCGCCCAGCGCGGCGCCCGAACGGCGGAAGGCTTTGCGCGC
CTCGATAGTCTGGTGCGCGAATGGGCGATCCCTCTGGTGGAATACTGGGGCACCGAGGTTACCCTGAGCGCCGATAATCC
CCTGCTCGCCGGGGCTGATCCCAGCGTCTGGCTCGCTGACGCCGATGTGATTCTGGTGGTCGACTCGCAGGCACCATGGA
TGATCGCCGAGGGCGACTGTCGCCAGGACTGCACCGTGATCCAGATGGGGCCCGATCCGCTCTTCTCCCGCTACCCGGTG
CGCGGCTATCGCGCGGACATCAACCTGGCGGGCGAGACCGATGAAGTCTTCAGCCTGCTGGAAGAGGCGCTGCGCCCGCT
GCAGGCCGCCCGCCAGCAGCATGTGGCGGAGCGGGCGGCATATACGCAGAGCCGCATCCAGCAACAGAAAAACCAGCGCG
ACGCGCTTCTCCATGCCAGCCAGACCGGGGCCATTACCAAACCCTGGCTCAGCTACTGCCTCGGCCATCTGGCTAATCAA
CACCGCGGCCGGATCGTTTCTGAACTGACCACGCTGCCGCAGTTTGCCGGACTGACCCATGCCGAGAGCTATTACCAGGA
AGCGCTGGCCGGCGGCCTGGGCGAAGCCCTGCCCATCGCGCTGGGGCTGCAGCTTGCGCGCCGGGAAGAGCTGATTATTG
CCGCCGTCGGCGACGGGTCGTACCTGTTCGCCAACCCTGCGGTCTGCCACCATATTGCGGAGGTAATGAAGCTTCCTGTC
CTCGTCGTGGTCGGAAATAACGGCGGATGGGGCGCCGTCGCGGGCGGCACCAAAGCGCTCTACCCGGACGGCTACGCGGC
CCGCGCGGAAACCATTCCGGCCACCGCCTTTACCACCTCGCCGGATTTCGCCGCGATCGCCGCCTCCAGCCGCGCGGCGG
CCCTGTCGGTTTCCCGGGCCGAAGATCTCCCCGGCGTGCTGGAAGAAGCGGTATCCCTCATACGCACCCGCCGACAGAGC
GTGCTGGTTGACGTGCAGTTAGCCCGCTAA

Upstream 100 bases:

>100_bases
CGATGGCGCTGAGTATTATTGCCTCCATCATTACGATGGGCGGATTTATTTTCTTAACCTTTTAATTACCGCGATTACAC
CGCGTGAATACGGAGAGAAT

Downstream 100 bases:

>100_bases
CGGCTTCTCCTCCGGGCGCTTGTCGTCCGGAGGGGGTTGTCACCCCTTCTGGTCACCTTTATCCACATGGCCCAGCGGCT
TCTCCGGGAAGCAGACATCG

Product: acetolactate synthase catalytic subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 569; Mature: 568

Protein sequence:

>569_residues
MASITLSAAELLLHRLQALDVAYIFINSGTDYPPVIEAWAKARATGQKVPELVICPHENAAIGMAHGYYLGTGKVQAVMV
HTNVGLANAACGVINLANSNIPVLIFGGRTPISEHSHFGCRNTPIGYGQEMRDQAALIRESVKWDFELRLADQIGEHVDR
AWAIASSLPKGPVYLSLPREPLCETFAVDEAALQAGPSQQPVRYAPVREDIARAAEAIACARHPVIFAQRGARTAEGFAR
LDSLVREWAIPLVEYWGTEVTLSADNPLLAGADPSVWLADADVILVVDSQAPWMIAEGDCRQDCTVIQMGPDPLFSRYPV
RGYRADINLAGETDEVFSLLEEALRPLQAARQQHVAERAAYTQSRIQQQKNQRDALLHASQTGAITKPWLSYCLGHLANQ
HRGRIVSELTTLPQFAGLTHAESYYQEALAGGLGEALPIALGLQLARREELIIAAVGDGSYLFANPAVCHHIAEVMKLPV
LVVVGNNGGWGAVAGGTKALYPDGYAARAETIPATAFTTSPDFAAIAASSRAAALSVSRAEDLPGVLEEAVSLIRTRRQS
VLVDVQLAR

Sequences:

>Translated_569_residues
MASITLSAAELLLHRLQALDVAYIFINSGTDYPPVIEAWAKARATGQKVPELVICPHENAAIGMAHGYYLGTGKVQAVMV
HTNVGLANAACGVINLANSNIPVLIFGGRTPISEHSHFGCRNTPIGYGQEMRDQAALIRESVKWDFELRLADQIGEHVDR
AWAIASSLPKGPVYLSLPREPLCETFAVDEAALQAGPSQQPVRYAPVREDIARAAEAIACARHPVIFAQRGARTAEGFAR
LDSLVREWAIPLVEYWGTEVTLSADNPLLAGADPSVWLADADVILVVDSQAPWMIAEGDCRQDCTVIQMGPDPLFSRYPV
RGYRADINLAGETDEVFSLLEEALRPLQAARQQHVAERAAYTQSRIQQQKNQRDALLHASQTGAITKPWLSYCLGHLANQ
HRGRIVSELTTLPQFAGLTHAESYYQEALAGGLGEALPIALGLQLARREELIIAAVGDGSYLFANPAVCHHIAEVMKLPV
LVVVGNNGGWGAVAGGTKALYPDGYAARAETIPATAFTTSPDFAAIAASSRAAALSVSRAEDLPGVLEEAVSLIRTRRQS
VLVDVQLAR
>Mature_568_residues
ASITLSAAELLLHRLQALDVAYIFINSGTDYPPVIEAWAKARATGQKVPELVICPHENAAIGMAHGYYLGTGKVQAVMVH
TNVGLANAACGVINLANSNIPVLIFGGRTPISEHSHFGCRNTPIGYGQEMRDQAALIRESVKWDFELRLADQIGEHVDRA
WAIASSLPKGPVYLSLPREPLCETFAVDEAALQAGPSQQPVRYAPVREDIARAAEAIACARHPVIFAQRGARTAEGFARL
DSLVREWAIPLVEYWGTEVTLSADNPLLAGADPSVWLADADVILVVDSQAPWMIAEGDCRQDCTVIQMGPDPLFSRYPVR
GYRADINLAGETDEVFSLLEEALRPLQAARQQHVAERAAYTQSRIQQQKNQRDALLHASQTGAITKPWLSYCLGHLANQH
RGRIVSELTTLPQFAGLTHAESYYQEALAGGLGEALPIALGLQLARREELIIAAVGDGSYLFANPAVCHHIAEVMKLPVL
VVVGNNGGWGAVAGGTKALYPDGYAARAETIPATAFTTSPDFAAIAASSRAAALSVSRAEDLPGVLEEAVSLIRTRRQSV
LVDVQLAR

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081685, Length=584, Percent_Identity=25.1712328767123, Blast_Score=97, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6320816, Length=582, Percent_Identity=20.9621993127148, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 61259; Mature: 61128

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASITLSAAELLLHRLQALDVAYIFINSGTDYPPVIEAWAKARATGQKVPELVICPHENA
CCEEEHHHHHHHHHHHHHCEEEEEEEECCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCC
AIGMAHGYYLGTGKVQAVMVHTNVGLANAACGVINLANSNIPVLIFGGRTPISEHSHFGC
EEEEECCEEEECCCEEEEEEEECCCCCHHCCCEEEECCCCCCEEEECCCCCCCCCCCCCC
RNTPIGYGQEMRDQAALIRESVKWDFELRLADQIGEHVDRAWAIASSLPKGPVYLSLPRE
CCCCCCCCHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCEEEECCCC
PLCETFAVDEAALQAGPSQQPVRYAPVREDIARAAEAIACARHPVIFAQRGARTAEGFAR
HHHHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHH
LDSLVREWAIPLVEYWGTEVTLSADNPLLAGADPSVWLADADVILVVDSQAPWMIAEGDC
HHHHHHHHHHHHHHHCCCEEEEECCCCEEECCCCCEEEECCCEEEEECCCCCEEEECCCC
RQDCTVIQMGPDPLFSRYPVRGYRADINLAGETDEVFSLLEEALRPLQAARQQHVAERAA
CCCCEEEEECCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YTQSRIQQQKNQRDALLHASQTGAITKPWLSYCLGHLANQHRGRIVSELTTLPQFAGLTH
HHHHHHHHHHCHHHHHEECCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHH
AESYYQEALAGGLGEALPIALGLQLARREELIIAAVGDGSYLFANPAVCHHIAEVMKLPV
HHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEEEECCCCEEEECHHHHHHHHHHHCCCE
LVVVGNNGGWGAVAGGTKALYPDGYAARAETIPATAFTTSPDFAAIAASSRAAALSVSRA
EEEEECCCCCEEECCCCCEECCCCCCCCCCCCCCEEEECCCCCCEEECCCCHHEEEHHHH
EDLPGVLEEAVSLIRTRRQSVLVDVQLAR
HCCHHHHHHHHHHHHHHHHHEEEEEEECC
>Mature Secondary Structure 
ASITLSAAELLLHRLQALDVAYIFINSGTDYPPVIEAWAKARATGQKVPELVICPHENA
CEEEHHHHHHHHHHHHHCEEEEEEEECCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCC
AIGMAHGYYLGTGKVQAVMVHTNVGLANAACGVINLANSNIPVLIFGGRTPISEHSHFGC
EEEEECCEEEECCCEEEEEEEECCCCCHHCCCEEEECCCCCCEEEECCCCCCCCCCCCCC
RNTPIGYGQEMRDQAALIRESVKWDFELRLADQIGEHVDRAWAIASSLPKGPVYLSLPRE
CCCCCCCCHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCEEEECCCC
PLCETFAVDEAALQAGPSQQPVRYAPVREDIARAAEAIACARHPVIFAQRGARTAEGFAR
HHHHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHH
LDSLVREWAIPLVEYWGTEVTLSADNPLLAGADPSVWLADADVILVVDSQAPWMIAEGDC
HHHHHHHHHHHHHHHCCCEEEEECCCCEEECCCCCEEEECCCEEEEECCCCCEEEECCCC
RQDCTVIQMGPDPLFSRYPVRGYRADINLAGETDEVFSLLEEALRPLQAARQQHVAERAA
CCCCEEEEECCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YTQSRIQQQKNQRDALLHASQTGAITKPWLSYCLGHLANQHRGRIVSELTTLPQFAGLTH
HHHHHHHHHHCHHHHHEECCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHH
AESYYQEALAGGLGEALPIALGLQLARREELIIAAVGDGSYLFANPAVCHHIAEVMKLPV
HHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEEEECCCCEEEECHHHHHHHHHHHCCCE
LVVVGNNGGWGAVAGGTKALYPDGYAARAETIPATAFTTSPDFAAIAASSRAAALSVSRA
EEEEECCCCCEEECCCCCEECCCCCCCCCCCCCCEEEECCCCCCEEECCCCHHEEEHHHH
EDLPGVLEEAVSLIRTRRQSVLVDVQLAR
HCCHHHHHHHHHHHHHHHHHEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]