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The map label for this gene is mak [H]

Identifier: 238896196

GI number: 238896196

Start: 4169333

End: 4170247

Strand: Direct

Name: mak [H]

Synonym: KP1_4345

Alternate gene names: 238896196

Gene position: 4169333-4170247 (Clockwise)

Preceding gene: 238896195

Following gene: 238896197

Centisome position: 79.44

GC content: 64.59

Gene sequence:

>915_bases
ATGTTACATCTTGGAATAGATATTGGCGGCACCAAAATGGAAGCCGTACTGCTGGACCCGGCGGGCGAATGCGTCCAGCG
CCTGCGCCGGCCGACCCACAAAGAGAGCTATGACGCCTTTATGCGTCAGCTGCTGACCCTGATTGCCGATATTCGCGCGG
TCAGCCCGCAGCCGTTCACCCTGGGGATCGGCCTGCCCGGGGCTATCGATCCGCAAAGCGGACGCATTAAAAACTGCAAC
TGCCTGGTGCTCAACGGTCACGATCTGCGCCGGGATATCATGCAGCAGCTTGGCCAGCCGGTGTGGATGGCAAACGATGC
CGACTGCTTCACTCTTTCGGAGGCGGTGGATGGCGCCGGCGCCGGAGCTACCACCGTCTTCGGGGTGATTATCGGTACCG
GCTGCGGCGGCGGGATCGCCGTTCATCAACAGCTGCTCAGCGGCCCCAACGCTATCGCCGGGGAGTGGGGGCATAATCCG
CTGCCGGGCTATACTCCTGAGCGCGATGGCCCGCCGCAGCCCTGCTACTGCGGCAAAACGAACTGCATCGAAAGCTTTCT
CTCCGGCACCGGCTTCGCCCGCCGCTATGGGGAACAAGCCCGTGCGGAAGCCATTGTCGCCGCGGCGCAAAACGGCGATC
CGCGCGCGCTGGCCCACTGGCGACACTTTATCGATGCCTTTGCCCGCAGCCTGGCGTCGGTGATCAATATTCTCGACCCG
CAGGTGATTGTGCTGGGCGGCGGTCTGTCCAACGTCAGCCAGATCTATCGCGATCTGCCTGCCGCCATCGTGCCGTGGAT
CTTCTCCGACACCTGCCGCACGCAGATCAAACCGGCCCGTTTCGGCGACGCCAGCGGCGTGCGCGGCGCGGCCTGGCTGC
CCCGGCTTCCCGGCGTCGCGCAAGGGCAGCGGTAG

Upstream 100 bases:

>100_bases
TGATTGCCGAACTGATTGCGATTTACCGCGCGCAGCCGCTTCACGCCTAGCGCCTTGCCCGGCCAGACGCGCTGGCCGGG
CCTGATTCCCCGAGGTCACT

Downstream 100 bases:

>100_bases
ACACTTTTTTCAGCAGGTATGTTAAGCTAGGCGAAAATGTTCAGACAGAAAAAGCCATGCCAACGATTGATGATGTTTCA
AGATTAGCGAATGTCTCAAG

Product: putative NAGC-like transcriptional regulator

Products: NA

Alternate protein names: D-fructose kinase; Manno(fructo)kinase [H]

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MLHLGIDIGGTKMEAVLLDPAGECVQRLRRPTHKESYDAFMRQLLTLIADIRAVSPQPFTLGIGLPGAIDPQSGRIKNCN
CLVLNGHDLRRDIMQQLGQPVWMANDADCFTLSEAVDGAGAGATTVFGVIIGTGCGGGIAVHQQLLSGPNAIAGEWGHNP
LPGYTPERDGPPQPCYCGKTNCIESFLSGTGFARRYGEQARAEAIVAAAQNGDPRALAHWRHFIDAFARSLASVINILDP
QVIVLGGGLSNVSQIYRDLPAAIVPWIFSDTCRTQIKPARFGDASGVRGAAWLPRLPGVAQGQR

Sequences:

>Translated_304_residues
MLHLGIDIGGTKMEAVLLDPAGECVQRLRRPTHKESYDAFMRQLLTLIADIRAVSPQPFTLGIGLPGAIDPQSGRIKNCN
CLVLNGHDLRRDIMQQLGQPVWMANDADCFTLSEAVDGAGAGATTVFGVIIGTGCGGGIAVHQQLLSGPNAIAGEWGHNP
LPGYTPERDGPPQPCYCGKTNCIESFLSGTGFARRYGEQARAEAIVAAAQNGDPRALAHWRHFIDAFARSLASVINILDP
QVIVLGGGLSNVSQIYRDLPAAIVPWIFSDTCRTQIKPARFGDASGVRGAAWLPRLPGVAQGQR
>Mature_304_residues
MLHLGIDIGGTKMEAVLLDPAGECVQRLRRPTHKESYDAFMRQLLTLIADIRAVSPQPFTLGIGLPGAIDPQSGRIKNCN
CLVLNGHDLRRDIMQQLGQPVWMANDADCFTLSEAVDGAGAGATTVFGVIIGTGCGGGIAVHQQLLSGPNAIAGEWGHNP
LPGYTPERDGPPQPCYCGKTNCIESFLSGTGFARRYGEQARAEAIVAAAQNGDPRALAHWRHFIDAFARSLASVINILDP
QVIVLGGGLSNVSQIYRDLPAAIVPWIFSDTCRTQIKPARFGDASGVRGAAWLPRLPGVAQGQR

Specific function: Catalyzes the phosphorylation of fructose to fructose-6- P. Has also low level glucokinase activity in vitro. Is not able to phosphorylate D-ribose, D-mannitol, D-sorbitol, inositol, and L-threonine [H]

COG id: COG1940

COG function: function code KG; Transcriptional regulator/sugar kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family [H]

Homologues:

Organism=Homo sapiens, GI298566325, Length=280, Percent_Identity=26.4285714285714, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI298566315, Length=280, Percent_Identity=26.4285714285714, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI4885285, Length=280, Percent_Identity=26.4285714285714, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI190014632, Length=280, Percent_Identity=26.4285714285714, Blast_Score=82, Evalue=5e-16,
Organism=Escherichia coli, GI87081733, Length=298, Percent_Identity=46.3087248322148, Blast_Score=263, Evalue=1e-71,
Organism=Escherichia coli, GI1787363, Length=299, Percent_Identity=36.1204013377926, Blast_Score=176, Evalue=1e-45,
Organism=Escherichia coli, GI1790522, Length=299, Percent_Identity=24.7491638795987, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1787878, Length=234, Percent_Identity=26.4957264957265, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI87082230, Length=262, Percent_Identity=29.3893129770992, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1786891, Length=240, Percent_Identity=24.5833333333333, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000600 [H]

Pfam domain/function: PF00480 ROK [H]

EC number: =2.7.1.4 [H]

Molecular weight: Translated: 32383; Mature: 32383

Theoretical pI: Translated: 7.42; Mature: 7.42

Prosite motif: PS01125 ROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHLGIDIGGTKMEAVLLDPAGECVQRLRRPTHKESYDAFMRQLLTLIADIRAVSPQPFT
CEEEEEECCCCEEEEEEECCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEE
LGIGLPGAIDPQSGRIKNCNCLVLNGHDLRRDIMQQLGQPVWMANDADCFTLSEAVDGAG
EECCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHCCCCEEECCCCCCEEHHHHHCCCC
AGATTVFGVIIGTGCGGGIAVHQQLLSGPNAIAGEWGHNPLPGYTPERDGPPQPCYCGKT
CCHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCH
NCIESFLSGTGFARRYGEQARAEAIVAAAQNGDPRALAHWRHFIDAFARSLASVINILDP
HHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
QVIVLGGGLSNVSQIYRDLPAAIVPWIFSDTCRTQIKPARFGDASGVRGAAWLPRLPGVA
CEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHCCCCCCCC
QGQR
CCCC
>Mature Secondary Structure
MLHLGIDIGGTKMEAVLLDPAGECVQRLRRPTHKESYDAFMRQLLTLIADIRAVSPQPFT
CEEEEEECCCCEEEEEEECCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEE
LGIGLPGAIDPQSGRIKNCNCLVLNGHDLRRDIMQQLGQPVWMANDADCFTLSEAVDGAG
EECCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHCCCCEEECCCCCCEEHHHHHCCCC
AGATTVFGVIIGTGCGGGIAVHQQLLSGPNAIAGEWGHNPLPGYTPERDGPPQPCYCGKT
CCHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCH
NCIESFLSGTGFARRYGEQARAEAIVAAAQNGDPRALAHWRHFIDAFARSLASVINILDP
HHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
QVIVLGGGLSNVSQIYRDLPAAIVPWIFSDTCRTQIKPARFGDASGVRGAAWLPRLPGVA
CEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHCCCCCCCC
QGQR
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8807285; 9278503; 1744033 [H]