Click here to switch to the map view.

The map label for this gene is hycD [H]

Identifier: 238896179

GI number: 238896179

Start: 4150520

End: 4151443

Strand: Reverse

Name: hycD [H]

Synonym: KP1_4326

Alternate gene names: 238896179

Gene position: 4151443-4150520 (Counterclockwise)

Preceding gene: 238896180

Following gene: 238896178

Centisome position: 79.1

GC content: 62.66

Gene sequence:

>924_bases
ATGAGTCTGTTAATTGCATTGCTACAGGCGCTGGTGCTGTTTGCCGTCGCGCCGCTGTTGTCCGGGATCGTTCGCGTCGC
CCGCGCGCGGCTGCATAACCGCCGTGGACCGGGGGTACTGCAGGAGTATCGCGATATCCTGAAACTGCTGGGCCGCCAGA
GCGTCGGTCCTGACGCCTCGGGCTGGGTGTTTCGCCTGACGCCGTACGTCATGGTGGGCGTGATGCTGACCATCGCCACC
GCGCTGCCGGTGGTGACGGTAGACTCCCCGCTGCCGGTGCTGGGGGATCTCATCACCCTGATCTACCTGTTCGCCATCGC
CCGTTTCTTCTTTGCCATTTCCGGTCTGGACACCGGCAGCCCGTTTACCGCGCTGGGCAGCAGTCGCGAAGCGATGCTCG
GCGTGCTGGTGGAGCCGATCCTGCTGTTGGGCCTGTGGGTGGCGGCGCAGGTCGCCGGCTCGACCCATATCAGCGCCATC
ACCGATACCCTTTACCACTGGCCGGCGGCGCGCAGCATTCCACTGATTCTGGCGTTATGCGCCTGCGCCTTCGCCACCTT
TATCGAGATGGGCAAACTGCCGTTCGATCTGGCGGAAGCGGAGCAGGAGCTGCAGGAAGGGCCGCTGTCGGAATACAGCG
GTAGCGGCTTTGGCATCCTGAAGTGGGGCATCAGCCTCAAACAGCTGGTGGTGCTGCAGATGTTTGTCGGGGTCTTCTTT
CCGTGGGGCCAGATGACCAGCTTCAGCGTCGGCGGCCTGCTGCTGGCGCTGGTGGTCGCCATCGTTAAGCTGGTGGTTGG
GGTGCTAATTATCGCGCTGTTTGAAAACAGCATGGCGCGTCTGCGTTTTTGTGCCACCTCCCGCGTCACCTGGGCCGGGT
TTGGTTTCGCCTTTTTAGCTTTCGTCTCCTTGCTGGTGGCGTGA

Upstream 100 bases:

>100_bases
CGGGCTGGCAATCGGCCTCGGCGCCGGCGCTGCTGCGCGGGATCGCGCTGGTTGAGCTGGCGGTGCTGGTGGTGATTGTC
ATTTCGCGAGGAGCCTGAGA

Downstream 100 bases:

>100_bases
TTAAGAGAGTTTGAGCATGTCTGAAGAAAAAATCGGTCAACACTACCTTGCGGCGCTGCACCAGGCCTTTCCCGGCGTGG
TGCTGGACGAAGCCTGGCAG

Product: hydrogenase 3 membrane subunit

Products: NA

Alternate protein names: FHL subunit 4; Hydrogenase 3 component D [H]

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MSLLIALLQALVLFAVAPLLSGIVRVARARLHNRRGPGVLQEYRDILKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIAT
ALPVVTVDSPLPVLGDLITLIYLFAIARFFFAISGLDTGSPFTALGSSREAMLGVLVEPILLLGLWVAAQVAGSTHISAI
TDTLYHWPAARSIPLILALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFGILKWGISLKQLVVLQMFVGVFF
PWGQMTSFSVGGLLLALVVAIVKLVVGVLIIALFENSMARLRFCATSRVTWAGFGFAFLAFVSLLVA

Sequences:

>Translated_307_residues
MSLLIALLQALVLFAVAPLLSGIVRVARARLHNRRGPGVLQEYRDILKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIAT
ALPVVTVDSPLPVLGDLITLIYLFAIARFFFAISGLDTGSPFTALGSSREAMLGVLVEPILLLGLWVAAQVAGSTHISAI
TDTLYHWPAARSIPLILALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFGILKWGISLKQLVVLQMFVGVFF
PWGQMTSFSVGGLLLALVVAIVKLVVGVLIIALFENSMARLRFCATSRVTWAGFGFAFLAFVSLLVA
>Mature_306_residues
SLLIALLQALVLFAVAPLLSGIVRVARARLHNRRGPGVLQEYRDILKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATA
LPVVTVDSPLPVLGDLITLIYLFAIARFFFAISGLDTGSPFTALGSSREAMLGVLVEPILLLGLWVAAQVAGSTHISAIT
DTLYHWPAARSIPLILALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFGILKWGISLKQLVVLQMFVGVFFP
WGQMTSFSVGGLLLALVVAIVKLVVGVLIIALFENSMARLRFCATSRVTWAGFGFAFLAFVSLLVA

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG0650

COG function: function code C; Formate hydrogenlyase subunit 4

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 1 family [H]

Homologues:

Organism=Escherichia coli, GI1789077, Length=307, Percent_Identity=88.9250814332247, Blast_Score=546, Evalue=1e-157,
Organism=Escherichia coli, GI87082115, Length=301, Percent_Identity=53.4883720930233, Blast_Score=285, Evalue=3e-78,
Organism=Escherichia coli, GI1788618, Length=306, Percent_Identity=26.1437908496732, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001694
- InterPro:   IPR018086 [H]

Pfam domain/function: PF00146 NADHdh [H]

EC number: NA

Molecular weight: Translated: 32957; Mature: 32826

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: PS00667 COMPLEX1_ND1_1 ; PS00668 COMPLEX1_ND1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLLIALLQALVLFAVAPLLSGIVRVARARLHNRRGPGVLQEYRDILKLLGRQSVGPDAS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCC
GWVFRLTPYVMVGVMLTIATALPVVTVDSPLPVLGDLITLIYLFAIARFFFAISGLDTGS
CHHHHHHHHHHHHHHHHHHHHHCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PFTALGSSREAMLGVLVEPILLLGLWVAAQVAGSTHISAITDTLYHWPAARSIPLILALC
CHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHH
ACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFGILKWGISLKQLVVLQMFVGVFF
HHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHC
PWGQMTSFSVGGLLLALVVAIVKLVVGVLIIALFENSMARLRFCATSRVTWAGFGFAFLA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
FVSLLVA
HHHHHHC
>Mature Secondary Structure 
SLLIALLQALVLFAVAPLLSGIVRVARARLHNRRGPGVLQEYRDILKLLGRQSVGPDAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCC
GWVFRLTPYVMVGVMLTIATALPVVTVDSPLPVLGDLITLIYLFAIARFFFAISGLDTGS
CHHHHHHHHHHHHHHHHHHHHHCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PFTALGSSREAMLGVLVEPILLLGLWVAAQVAGSTHISAITDTLYHWPAARSIPLILALC
CHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHH
ACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFGILKWGISLKQLVVLQMFVGVFF
HHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHC
PWGQMTSFSVGGLLLALVVAIVKLVVGVLIIALFENSMARLRFCATSRVTWAGFGFAFLA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
FVSLLVA
HHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2187144; 9278503 [H]