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The map label for this gene is ygaA [H]

Identifier: 238896163

GI number: 238896163

Start: 4133614

End: 4135164

Strand: Reverse

Name: ygaA [H]

Synonym: KP1_4309

Alternate gene names: 238896163

Gene position: 4135164-4133614 (Counterclockwise)

Preceding gene: 238896167

Following gene: 238896155

Centisome position: 78.79

GC content: 63.96

Gene sequence:

>1551_bases
ATGAGTTTTTCCGTCGACGAGCTGGCGAGAATCGCCATAGATTTACAAAGCGATATTGGTCACACCGACCGCTTTTCGCG
CCTGATTACTACCCTGCGGCAAATTCTTGGCTGCGATGCCTCGGCGCTGCTGCGCTATGAGGCGCATCAGTTTGTGCCGC
TGGCGATCGATGGCCTGGCGCAGGATGTGCTCGGTCGGCGCTTTGCCCTGGAAGGACACCCGCGGCTGGAGGCGATTGCG
CGCGCCGGGGATGTGGTGCGCTTCCCGGCGGATAGCGACCTGCCCGACCCCTATGACGGGCTGATCCCCGGTCATGAGAG
CCTCAAAGTCCATGCCTGCGTGGGACTGCCGCTGTTTGCCGGGCAGACGCTGATTGGCGCGCTGACCCTGGACGGCATGG
ATGCCGATCGTTTCGATAGCTTCAGCGATGAAGAGCTGCGGCTGATTGCCGCGCTGGTGGCCGGGGCGCTGAATAATGCG
CTGCTTATCGCCCGCCTTGAAGCGCAAAACGTCCTGCCGGCGCAGGCGGTGAATTACCCGCTGCCGGAGCGCCAGGAGAT
CATCGGCCTGTCGGGGCCCATGCTGCAGCTGAAAAAAGAGATCGATATCGTCACCGCCTCGGATTTAAACGTGCTGATCA
GCGGCGAAACCGGCACCGGCAAAGAGCTGGTGGCGAAGGCGGTGCATCAGGGGTCGCCGCGAGCCGCCAACCCTCTGGTG
TACCTCAACTGCGCGGCGCTGCCGGAAAGCGTAGCGGAGAGCGAGCTGTTCGGCCATGTGAAAGGGGCATTTACCGGGGC
CATCAGCAACCGCAGCGGTAAATTTGAGATGGCGGATAACGGCACCCTGTTTCTTGACGAAATCGGCGAACTGTCGCTGG
CGCTGCAGGCCAAACTGCTGCGCGTGCTGCAGTATGGCGATATTCAGCGCGTCGGCGATGACCGCAGCCTGCGGGTCGAT
GTGCGGGTGCTGGCGGCGACGAACCGCGATCTGCGCCAGGAGGTGGTGGAAGGGCGCTTTCGCGCCGACCTGTACCATCG
GCTGAGCGTCTTCCCGCTGTCGGTTCCGCCGCTACGCGAGCGGGAAAGTGACGTGGTGCTGCTGGCGGGCTATTTCTGCG
AGCAGTGCCGCCTGCGGATGGGGCTGGCGCGGGTCATTTTGGCTGAGGCGGCGCGAAACCGGCTGCAGCAGTGGTCCTGG
CCGGGCAACGTGCGCGAACTGGAGCATGCTATCCATCGGGCGGTGGTGCTGGCGCGGGCCACCCAGGCCGGTGATGAAGT
GGTCCTGGAGCCGCAGCATTTCCAGTTTGCGGTGGCGGCGCCGATGTTGCCGACTGAGACGGCGGCAGCGGCGCCCGCGA
CGGGAAACATCAATCTGCGCGAGGCGACCGACTCTTTTCAGCGAGAAGCCATTAGCCGTGCGCTGGAGGCGAACCAGGGC
AACTGGGCGGCGACCGCCCGAGCGCTGGAACTGGACGTCGCCAACCTGCATCGGCTGGCGAAACGTCTGGGGCTTAAAGG
ATCCCCGCCTGGTAAAAGTTCTGCAGGTTAA

Upstream 100 bases:

>100_bases
TCTGTTTTTAATTTTTTCTGATTTTGGTTGTTGTCAAAATGACAATGGTTAAAATAGTCGATATGACAATGCGTATTGTC
AAAATGACAATGGAGCGGCA

Downstream 100 bases:

>100_bases
TGGCGCCGACCAGTTGGCCGTTCTCATCCACTACCGGCGCGGCGCTGATTTTGTGTTTCATCAGCCGCTCTTTGGCTTCC
ACCGCGCGGCTATCCGCCTG

Product: anaerobic nitric oxide reductase transcription regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 516; Mature: 515

Protein sequence:

>516_residues
MSFSVDELARIAIDLQSDIGHTDRFSRLITTLRQILGCDASALLRYEAHQFVPLAIDGLAQDVLGRRFALEGHPRLEAIA
RAGDVVRFPADSDLPDPYDGLIPGHESLKVHACVGLPLFAGQTLIGALTLDGMDADRFDSFSDEELRLIAALVAGALNNA
LLIARLEAQNVLPAQAVNYPLPERQEIIGLSGPMLQLKKEIDIVTASDLNVLISGETGTGKELVAKAVHQGSPRAANPLV
YLNCAALPESVAESELFGHVKGAFTGAISNRSGKFEMADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVD
VRVLAATNRDLRQEVVEGRFRADLYHRLSVFPLSVPPLRERESDVVLLAGYFCEQCRLRMGLARVILAEAARNRLQQWSW
PGNVRELEHAIHRAVVLARATQAGDEVVLEPQHFQFAVAAPMLPTETAAAAPATGNINLREATDSFQREAISRALEANQG
NWAATARALELDVANLHRLAKRLGLKGSPPGKSSAG

Sequences:

>Translated_516_residues
MSFSVDELARIAIDLQSDIGHTDRFSRLITTLRQILGCDASALLRYEAHQFVPLAIDGLAQDVLGRRFALEGHPRLEAIA
RAGDVVRFPADSDLPDPYDGLIPGHESLKVHACVGLPLFAGQTLIGALTLDGMDADRFDSFSDEELRLIAALVAGALNNA
LLIARLEAQNVLPAQAVNYPLPERQEIIGLSGPMLQLKKEIDIVTASDLNVLISGETGTGKELVAKAVHQGSPRAANPLV
YLNCAALPESVAESELFGHVKGAFTGAISNRSGKFEMADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVD
VRVLAATNRDLRQEVVEGRFRADLYHRLSVFPLSVPPLRERESDVVLLAGYFCEQCRLRMGLARVILAEAARNRLQQWSW
PGNVRELEHAIHRAVVLARATQAGDEVVLEPQHFQFAVAAPMLPTETAAAAPATGNINLREATDSFQREAISRALEANQG
NWAATARALELDVANLHRLAKRLGLKGSPPGKSSAG
>Mature_515_residues
SFSVDELARIAIDLQSDIGHTDRFSRLITTLRQILGCDASALLRYEAHQFVPLAIDGLAQDVLGRRFALEGHPRLEAIAR
AGDVVRFPADSDLPDPYDGLIPGHESLKVHACVGLPLFAGQTLIGALTLDGMDADRFDSFSDEELRLIAALVAGALNNAL
LIARLEAQNVLPAQAVNYPLPERQEIIGLSGPMLQLKKEIDIVTASDLNVLISGETGTGKELVAKAVHQGSPRAANPLVY
LNCAALPESVAESELFGHVKGAFTGAISNRSGKFEMADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDV
RVLAATNRDLRQEVVEGRFRADLYHRLSVFPLSVPPLRERESDVVLLAGYFCEQCRLRMGLARVILAEAARNRLQQWSWP
GNVRELEHAIHRAVVLARATQAGDEVVLEPQHFQFAVAAPMLPTETAAAAPATGNINLREATDSFQREAISRALEANQGN
WAATARALELDVANLHRLAKRLGLKGSPPGKSSAG

Specific function: Required for the expression of anaerobic nitric oxide (NO) reductase, acts as a transcriptional activator for at least the norVW operon. Activation also requires sigma-54 [H]

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI87082152, Length=506, Percent_Identity=80.4347826086957, Blast_Score=778, Evalue=0.0,
Organism=Escherichia coli, GI87082117, Length=314, Percent_Identity=49.3630573248408, Blast_Score=270, Evalue=1e-73,
Organism=Escherichia coli, GI1789087, Length=423, Percent_Identity=40.4255319148936, Blast_Score=268, Evalue=4e-73,
Organism=Escherichia coli, GI1788550, Length=303, Percent_Identity=45.5445544554455, Blast_Score=249, Evalue=3e-67,
Organism=Escherichia coli, GI1790437, Length=303, Percent_Identity=46.2046204620462, Blast_Score=240, Evalue=1e-64,
Organism=Escherichia coli, GI1790299, Length=335, Percent_Identity=42.6865671641791, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI1788905, Length=319, Percent_Identity=39.4984326018809, Blast_Score=230, Evalue=2e-61,
Organism=Escherichia coli, GI1789233, Length=328, Percent_Identity=39.0243902439024, Blast_Score=225, Evalue=6e-60,
Organism=Escherichia coli, GI87081872, Length=329, Percent_Identity=38.2978723404255, Blast_Score=211, Evalue=1e-55,
Organism=Escherichia coli, GI1787583, Length=320, Percent_Identity=38.4375, Blast_Score=197, Evalue=2e-51,
Organism=Escherichia coli, GI1786524, Length=302, Percent_Identity=40.0662251655629, Blast_Score=194, Evalue=9e-51,
Organism=Escherichia coli, GI1789828, Length=226, Percent_Identity=38.4955752212389, Blast_Score=141, Evalue=8e-35,
Organism=Escherichia coli, GI87081858, Length=300, Percent_Identity=31, Blast_Score=119, Evalue=5e-28,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR009057
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 55954; Mature: 55822

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFSVDELARIAIDLQSDIGHTDRFSRLITTLRQILGCDASALLRYEAHQFVPLAIDGLA
CCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCEEEHHHHHHH
QDVLGRRFALEGHPRLEAIARAGDVVRFPADSDLPDPYDGLIPGHESLKVHACVGLPLFA
HHHHCCEEECCCCCHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCCEEEEEEECCCHHC
GQTLIGALTLDGMDADRFDSFSDEELRLIAALVAGALNNALLIARLEAQNVLPAQAVNYP
CCCEEEEEEECCCCHHHCCCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHCCCC
LPERQEIIGLSGPMLQLKKEIDIVTASDLNVLISGETGTGKELVAKAVHQGSPRAANPLV
CCCCHHHCCCCCCHHHHHHHCCEEEECCCEEEEECCCCCCHHHHHHHHHCCCCCCCCCEE
YLNCAALPESVAESELFGHVKGAFTGAISNRSGKFEMADNGTLFLDEIGELSLALQAKLL
EEEECCCCHHHHHHHHHHHHHHHEEEEECCCCCCEEECCCCEEEEECHHHHHHHHHHHHH
RVLQYGDIQRVGDDRSLRVDVRVLAATNRDLRQEVVEGRFRADLYHRLSVFPLSVPPLRE
HHHHCCCHHHCCCCCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCC
RESDVVLLAGYFCEQCRLRMGLARVILAEAARNRLQQWSWPGNVRELEHAIHRAVVLARA
CCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
TQAGDEVVLEPQHFQFAVAAPMLPTETAAAAPATGNINLREATDSFQREAISRALEANQG
HCCCCCEEECCCCCEEEEECCCCCCCHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCC
NWAATARALELDVANLHRLAKRLGLKGSPPGKSSAG
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
SFSVDELARIAIDLQSDIGHTDRFSRLITTLRQILGCDASALLRYEAHQFVPLAIDGLA
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCEEEHHHHHHH
QDVLGRRFALEGHPRLEAIARAGDVVRFPADSDLPDPYDGLIPGHESLKVHACVGLPLFA
HHHHCCEEECCCCCHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCCEEEEEEECCCHHC
GQTLIGALTLDGMDADRFDSFSDEELRLIAALVAGALNNALLIARLEAQNVLPAQAVNYP
CCCEEEEEEECCCCHHHCCCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHCCCC
LPERQEIIGLSGPMLQLKKEIDIVTASDLNVLISGETGTGKELVAKAVHQGSPRAANPLV
CCCCHHHCCCCCCHHHHHHHCCEEEECCCEEEEECCCCCCHHHHHHHHHCCCCCCCCCEE
YLNCAALPESVAESELFGHVKGAFTGAISNRSGKFEMADNGTLFLDEIGELSLALQAKLL
EEEECCCCHHHHHHHHHHHHHHHEEEEECCCCCCEEECCCCEEEEECHHHHHHHHHHHHH
RVLQYGDIQRVGDDRSLRVDVRVLAATNRDLRQEVVEGRFRADLYHRLSVFPLSVPPLRE
HHHHCCCHHHCCCCCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCC
RESDVVLLAGYFCEQCRLRMGLARVILAEAARNRLQQWSWPGNVRELEHAIHRAVVLARA
CCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
TQAGDEVVLEPQHFQFAVAAPMLPTETAAAAPATGNINLREATDSFQREAISRALEANQG
HCCCCCEEECCCCCEEEEECCCCCCCHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCC
NWAATARALELDVANLHRLAKRLGLKGSPPGKSSAG
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA