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The map label for this gene is nrdE [H]

Identifier: 238896130

GI number: 238896130

Start: 4099319

End: 4101460

Strand: Direct

Name: nrdE [H]

Synonym: KP1_4269

Alternate gene names: 238896130

Gene position: 4099319-4101460 (Clockwise)

Preceding gene: 238896129

Following gene: 238896131

Centisome position: 78.1

GC content: 61.3

Gene sequence:

>2142_bases
TTGGCAACGACAACCGCAGAACGTATAACCGCCGCCGTGGATTTTCACGCCCTCAACGCGATGCTGAATTTGTACGACAG
CGAGGGCCGCATCCCGTTTGAGAAAGATCGCCAGGCGGTTGAGGCCTTTATGGCGACCCAGGTTCAGCCCAACACCCTCA
CCTTCCCCAGCCAGGAGGATAAGCTCAGCTGGCTCGTCAGCGAGGGCTATTACGATCCGCAGGTGCTGGCCGGCTATGAC
CGCGGGTTTGTGCTGGCGTTGTTCGCCCATGCCCGGCGCGCTCCGTTTCGCTTTCAGACCTTCCTCGGCGCCTGGAAGTT
CTATACCAGCTATGCGCTGAAGACCTTTGACGGCAAGCACTACCTCGAGGATTTCGCCGAACGCAGCGTGATGGTGGCCC
TGACGCTGGCCCGGGGCGATGAACAGCAGGCGCGGCAGCTGACGGAGGAGATACTCAGCGGCCGCTTTCAGCCCGCCACA
CCGACCTTCCTTAACGCTGGCAAACAGCAGCGCGGGGAGCTGATCTCCTGCTTCCTGCTGCGTATTGAGGATAATATGGA
GTCCATCGGCCGGGCGGTGAACTCGGCGCTACAGCTGTCGAAGCGCGGCGGCGGGGTGGCCTTTTTACTCTCCAACCTGC
GCGAGGCCGGGGCGCCGATCAAGCGCATCGAAAACCAGTCTTCTGGGGTGGTGCCGGTGATGAAGATGCTGGAGGACGCC
TTCTCCTATGCCAATCAGCTCGGCGCCCGCCAGGGGGCGGGTGCGGTCTGGCTGCATGTTCACCATCCCGATATTTTACG
CTTTCTTGATACCCGGCGTGAAAATGCCGATGAGAAAATCCGCATCAAAACCTTGTCGCTGGGGGTGGTGATCCCTGATA
TCACCTTCCAGCTGGCGAAAGAAGATGCGCAGATGGCCCTGTTCTCGCCTTATGACGTCGAACGGCTGTACGGCAAACCT
TTCGCCGATTGCGCCATCGGCGACCTCTATCCGCAGCTGGTGGCCGACGAGCGGGTCCGCAAACGCTGGATCCGCGCCCG
CGACCTGTTCCAGCGCCTGGCGGAGATCCAGTTTGAATCCGGCTACCCGTACATCATGTTTGAAGACACCGTGAACCGCG
CCAGTCCGGTGGCCGGGCGGGTCACCATGAGCAATCTGTGTTCGGAGATCCTGCAGGTCAGTACGCCGTCCGCCTATAAT
GAAGACCTCAGCTATGCCCATATCGGGGAAGATATCTCCTGCAACCTCGGCTCGCTGAACATCGCCCATACCATGGACTC
GCCGGATTTCGGCCGCACAATCGCCACCGCGGTGCGCGCCCTGACCGCCGTGTCCGACATGAGCCACATTCAGTCGGTTC
CCTCGGTGGCGGCCGGCAACGCGGCCTCCCACGCCATCGGCCTGGGGCAGATGAATCTGCACGGTTACCTGGCGCGGGAG
GGCATTGCCTACGGCAGCCCGGAGGGGCTGGACTTCACCAATATCTACTTCTACACCGTGACCTGGCACGCGCTGCACAC
CTCGATGCAGCTGGCCCGCGAGCGCGGCCAGCGCTTCGCCGGCTTCGAGCAGTCGCGCTACGCCAGCGGGGCGTACTTCG
ACAAATATCTGCAGGAGGAGTGGCAGCCGAAAACCGAACGGGTGCGCACGCTGTTCGCCCGCGCCGGCATCTCTCTGCCC
GACCGGGAAAGCTGGCGCCAGCTGCGCGACGATGTCATGCGCTATGGCATCTACAATCGCTATCTGCAGGCGGTGCCGCC
CACCGGCTCGATCTCGTACATCAACCATGCCACCTCCAGCATTCACCCGATCGTCTCGAAGATTGAGATCCGCAAAGAGG
GGAAAACCGGGCGAGTGTACTACCCCGCGCCATTTATGAATAACGACAATCTGGCCCTCTACCAGGATGCGTATGAGATA
GGGCCGGAGAAGATCATTGACACTTACGCCGAGGCGACGCGCCACGTCGACCAGGGGCTGTCGCTGACCCTGTTCTTCCC
CGATACCGCCACCACCCGCGATATCAACAAGGCGCAAATTTACGCCTGGAAGAAAGGCATCAAGACGCTCTACTACATCC
GTCTGCGCCAGCTGGCGCTGGAAGGCACTGAAATTGAAGGCTGCGTGTCCTGCGCGCTGTAA

Upstream 100 bases:

>100_bases
ATGTCATCGCGCAAAAATGCGCCGTGCCCTATCTCTACCGCTTCGAGCTGATGGGAACCCCCGACGATATCAACACCGTG
CGTAAAGGAGTCAGCGAATT

Downstream 100 bases:

>100_bases
GGAGAACAGGATGACCCATTTAACGCGCGTCAGCGCAATCAACTGGAACCGCATTGATGACGATAAAGATCTTGAGGTCT
GGAACCGTCTCACCAGCAAC

Product: ribonucleotide-diphosphate reductase subunit alpha

Products: NA

Alternate protein names: R1E protein; Ribonucleotide reductase 2 [H]

Number of amino acids: Translated: 713; Mature: 712

Protein sequence:

>713_residues
MATTTAERITAAVDFHALNAMLNLYDSEGRIPFEKDRQAVEAFMATQVQPNTLTFPSQEDKLSWLVSEGYYDPQVLAGYD
RGFVLALFAHARRAPFRFQTFLGAWKFYTSYALKTFDGKHYLEDFAERSVMVALTLARGDEQQARQLTEEILSGRFQPAT
PTFLNAGKQQRGELISCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAGAPIKRIENQSSGVVPVMKMLEDA
FSYANQLGARQGAGAVWLHVHHPDILRFLDTRRENADEKIRIKTLSLGVVIPDITFQLAKEDAQMALFSPYDVERLYGKP
FADCAIGDLYPQLVADERVRKRWIRARDLFQRLAEIQFESGYPYIMFEDTVNRASPVAGRVTMSNLCSEILQVSTPSAYN
EDLSYAHIGEDISCNLGSLNIAHTMDSPDFGRTIATAVRALTAVSDMSHIQSVPSVAAGNAASHAIGLGQMNLHGYLARE
GIAYGSPEGLDFTNIYFYTVTWHALHTSMQLARERGQRFAGFEQSRYASGAYFDKYLQEEWQPKTERVRTLFARAGISLP
DRESWRQLRDDVMRYGIYNRYLQAVPPTGSISYINHATSSIHPIVSKIEIRKEGKTGRVYYPAPFMNNDNLALYQDAYEI
GPEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIYAWKKGIKTLYYIRLRQLALEGTEIEGCVSCAL

Sequences:

>Translated_713_residues
MATTTAERITAAVDFHALNAMLNLYDSEGRIPFEKDRQAVEAFMATQVQPNTLTFPSQEDKLSWLVSEGYYDPQVLAGYD
RGFVLALFAHARRAPFRFQTFLGAWKFYTSYALKTFDGKHYLEDFAERSVMVALTLARGDEQQARQLTEEILSGRFQPAT
PTFLNAGKQQRGELISCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAGAPIKRIENQSSGVVPVMKMLEDA
FSYANQLGARQGAGAVWLHVHHPDILRFLDTRRENADEKIRIKTLSLGVVIPDITFQLAKEDAQMALFSPYDVERLYGKP
FADCAIGDLYPQLVADERVRKRWIRARDLFQRLAEIQFESGYPYIMFEDTVNRASPVAGRVTMSNLCSEILQVSTPSAYN
EDLSYAHIGEDISCNLGSLNIAHTMDSPDFGRTIATAVRALTAVSDMSHIQSVPSVAAGNAASHAIGLGQMNLHGYLARE
GIAYGSPEGLDFTNIYFYTVTWHALHTSMQLARERGQRFAGFEQSRYASGAYFDKYLQEEWQPKTERVRTLFARAGISLP
DRESWRQLRDDVMRYGIYNRYLQAVPPTGSISYINHATSSIHPIVSKIEIRKEGKTGRVYYPAPFMNNDNLALYQDAYEI
GPEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIYAWKKGIKTLYYIRLRQLALEGTEIEGCVSCAL
>Mature_712_residues
ATTTAERITAAVDFHALNAMLNLYDSEGRIPFEKDRQAVEAFMATQVQPNTLTFPSQEDKLSWLVSEGYYDPQVLAGYDR
GFVLALFAHARRAPFRFQTFLGAWKFYTSYALKTFDGKHYLEDFAERSVMVALTLARGDEQQARQLTEEILSGRFQPATP
TFLNAGKQQRGELISCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAGAPIKRIENQSSGVVPVMKMLEDAF
SYANQLGARQGAGAVWLHVHHPDILRFLDTRRENADEKIRIKTLSLGVVIPDITFQLAKEDAQMALFSPYDVERLYGKPF
ADCAIGDLYPQLVADERVRKRWIRARDLFQRLAEIQFESGYPYIMFEDTVNRASPVAGRVTMSNLCSEILQVSTPSAYNE
DLSYAHIGEDISCNLGSLNIAHTMDSPDFGRTIATAVRALTAVSDMSHIQSVPSVAAGNAASHAIGLGQMNLHGYLAREG
IAYGSPEGLDFTNIYFYTVTWHALHTSMQLARERGQRFAGFEQSRYASGAYFDKYLQEEWQPKTERVRTLFARAGISLPD
RESWRQLRDDVMRYGIYNRYLQAVPPTGSISYINHATSSIHPIVSKIEIRKEGKTGRVYYPAPFMNNDNLALYQDAYEIG
PEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIYAWKKGIKTLYYIRLRQLALEGTEIEGCVSCAL

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. R1E contains the binding sites for both substrates and allosteric effectors and carries out the actual reductio

COG id: COG0209

COG function: function code F; Ribonucleotide reductase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase large chain family [H]

Homologues:

Organism=Homo sapiens, GI4506749, Length=643, Percent_Identity=26.7496111975117, Blast_Score=191, Evalue=2e-48,
Organism=Escherichia coli, GI1789030, Length=714, Percent_Identity=82.7731092436975, Blast_Score=1251, Evalue=0.0,
Organism=Escherichia coli, GI2367133, Length=581, Percent_Identity=25.3012048192771, Blast_Score=158, Evalue=9e-40,
Organism=Caenorhabditis elegans, GI17555242, Length=640, Percent_Identity=26.5625, Blast_Score=190, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6322123, Length=644, Percent_Identity=26.3975155279503, Blast_Score=185, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6320914, Length=645, Percent_Identity=26.5116279069767, Blast_Score=183, Evalue=7e-47,
Organism=Drosophila melanogaster, GI17352481, Length=643, Percent_Identity=24.4167962674961, Blast_Score=162, Evalue=6e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013346
- InterPro:   IPR013509
- InterPro:   IPR000788
- InterPro:   IPR008926
- InterPro:   IPR013554 [H]

Pfam domain/function: PF02867 Ribonuc_red_lgC; PF00317 Ribonuc_red_lgN; PF08343 RNR_N [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 80202; Mature: 80071

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS00089 RIBORED_LARGE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATTTAERITAAVDFHALNAMLNLYDSEGRIPFEKDRQAVEAFMATQVQPNTLTFPSQED
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEECCCCCCC
KLSWLVSEGYYDPQVLAGYDRGFVLALFAHARRAPFRFQTFLGAWKFYTSYALKTFDGKH
HHHHEECCCCCCCHHEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHH
YLEDFAERSVMVALTLARGDEQQARQLTEEILSGRFQPATPTFLNAGKQQRGELISCFLL
HHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCHHHHCCHHHHHHHHHHHHH
RIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAGAPIKRIENQSSGVVPVMKMLEDA
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHH
FSYANQLGARQGAGAVWLHVHHPDILRFLDTRRENADEKIRIKTLSLGVVIPDITFQLAK
HHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHCCCCCCEEEEEEEEEEEEECCHHHHHHH
EDAQMALFSPYDVERLYGKPFADCAIGDLYPQLVADERVRKRWIRARDLFQRLAEIQFES
CCCCEEEECCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEECC
GYPYIMFEDTVNRASPVAGRVTMSNLCSEILQVSTPSAYNEDLSYAHIGEDISCNLGSLN
CCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHCCCCCCCCCCCCEE
IAHTMDSPDFGRTIATAVRALTAVSDMSHIQSVPSVAAGNAASHAIGLGQMNLHGYLARE
EEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCHHHHEECCCCCHHHHHHHC
GIAYGSPEGLDFTNIYFYTVTWHALHTSMQLARERGQRFAGFEQSRYASGAYFDKYLQEE
CCCCCCCCCCCEEEEEEEEEEHHHHHHHHHHHHHHCHHHCCCCHHHCCCCHHHHHHHHHH
WQPKTERVRTLFARAGISLPDRESWRQLRDDVMRYGIYNRYLQAVPPTGSISYINHATSS
CCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
IHPIVSKIEIRKEGKTGRVYYPAPFMNNDNLALYQDAYEIGPEKIIDTYAEATRHVDQGL
HHHHHHHHHHEECCCCCEEEECCCCCCCCCEEEEHHHHHCCHHHHHHHHHHHHHHHHCCC
SLTLFFPDTATTRDINKAQIYAWKKGIKTLYYIRLRQLALEGTEIEGCVSCAL
EEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCC
>Mature Secondary Structure 
ATTTAERITAAVDFHALNAMLNLYDSEGRIPFEKDRQAVEAFMATQVQPNTLTFPSQED
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEECCCCCCC
KLSWLVSEGYYDPQVLAGYDRGFVLALFAHARRAPFRFQTFLGAWKFYTSYALKTFDGKH
HHHHEECCCCCCCHHEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHH
YLEDFAERSVMVALTLARGDEQQARQLTEEILSGRFQPATPTFLNAGKQQRGELISCFLL
HHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCHHHHCCHHHHHHHHHHHHH
RIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAGAPIKRIENQSSGVVPVMKMLEDA
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHH
FSYANQLGARQGAGAVWLHVHHPDILRFLDTRRENADEKIRIKTLSLGVVIPDITFQLAK
HHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHCCCCCCEEEEEEEEEEEEECCHHHHHHH
EDAQMALFSPYDVERLYGKPFADCAIGDLYPQLVADERVRKRWIRARDLFQRLAEIQFES
CCCCEEEECCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEECC
GYPYIMFEDTVNRASPVAGRVTMSNLCSEILQVSTPSAYNEDLSYAHIGEDISCNLGSLN
CCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHCCCCCCCCCCCCEE
IAHTMDSPDFGRTIATAVRALTAVSDMSHIQSVPSVAAGNAASHAIGLGQMNLHGYLARE
EEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCHHHHEECCCCCHHHHHHHC
GIAYGSPEGLDFTNIYFYTVTWHALHTSMQLARERGQRFAGFEQSRYASGAYFDKYLQEE
CCCCCCCCCCCEEEEEEEEEEHHHHHHHHHHHHHHCHHHCCCCHHHCCCCHHHHHHHHHH
WQPKTERVRTLFARAGISLPDRESWRQLRDDVMRYGIYNRYLQAVPPTGSISYINHATSS
CCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
IHPIVSKIEIRKEGKTGRVYYPAPFMNNDNLALYQDAYEIGPEKIIDTYAEATRHVDQGL
HHHHHHHHHHEECCCCCEEEECCCCCCCCCEEEEHHHHHCCHHHHHHHHHHHHHHHHCCC
SLTLFFPDTATTRDINKAQIYAWKKGIKTLYYIRLRQLALEGTEIEGCVSCAL
EEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 8820648; 2158980 [H]