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The map label for this gene is rob [H]

Identifier: 238896069

GI number: 238896069

Start: 4035617

End: 4035979

Strand: Reverse

Name: rob [H]

Synonym: KP1_4202

Alternate gene names: 238896069

Gene position: 4035979-4035617 (Counterclockwise)

Preceding gene: 238896072

Following gene: 238896067

Centisome position: 76.9

GC content: 57.58

Gene sequence:

>363_bases
ATGAACACTGGCGCCATCATTCAGGATCTGATTGACTGGATCGACAACCATCTTGATAGCCGTCTGGATATTGACACCGT
TGCCCGACGAGCCGGCTATTCGAAGTGGCACCTGCAGCGGATCTTCAAAGAACATACCGGGCAGCCCCTCGGAGAATATA
TTCGGGCGAAAAAGCTGCAAAAGTCGATCGAACGCTTGGCCCACAGCAACGAGCCGATCCTGAACGTGGCGATTGCCCTC
GGCTTTGACTCCCAGCAGTCCTTCAACCGCAGCTTCAAGCGCCAGTACGGCCAGGCGCCCGGCGTCTGGCGCCGGAGTAT
CAGCCGCTCTGTTGCGCAGACATCTCGTCAGCGATCCGCATGA

Upstream 100 bases:

>100_bases
ATTTAAATGTAACAATAAAACTTGCATTTTGTGTAAAAACAGGCCACCTTTAAATGCAACTGACAAAGATGCTTTTAACC
GGCCGCGAGGGGGTAACATG

Downstream 100 bases:

>100_bases
CCGGGGAGGGTCAGTCCTGACCCGGCCCATGTTCGAATTTGCTGCCTTTGCCGATGGCATAGAACTGCCCGCCTGCGACA
TAGTGAACGGTCCGTAGCTC

Product: putative helix-turn-helix AraC-type trancriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 120; Mature: 120

Protein sequence:

>120_residues
MNTGAIIQDLIDWIDNHLDSRLDIDTVARRAGYSKWHLQRIFKEHTGQPLGEYIRAKKLQKSIERLAHSNEPILNVAIAL
GFDSQQSFNRSFKRQYGQAPGVWRRSISRSVAQTSRQRSA

Sequences:

>Translated_120_residues
MNTGAIIQDLIDWIDNHLDSRLDIDTVARRAGYSKWHLQRIFKEHTGQPLGEYIRAKKLQKSIERLAHSNEPILNVAIAL
GFDSQQSFNRSFKRQYGQAPGVWRRSISRSVAQTSRQRSA
>Mature_120_residues
MNTGAIIQDLIDWIDNHLDSRLDIDTVARRAGYSKWHLQRIFKEHTGQPLGEYIRAKKLQKSIERLAHSNEPILNVAIAL
GFDSQQSFNRSFKRQYGQAPGVWRRSISRSVAQTSRQRSA

Specific function: Binds to the right arm of the replication origin oriC of the chromosome. Rob binding may influence the formation of the nucleoprotein structure, required for oriC function in the initiation of replication [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790857, Length=106, Percent_Identity=51.8867924528302, Blast_Score=123, Evalue=3e-30,
Organism=Escherichia coli, GI1790497, Length=104, Percent_Identity=48.0769230769231, Blast_Score=110, Evalue=2e-26,
Organism=Escherichia coli, GI87081928, Length=104, Percent_Identity=48.0769230769231, Blast_Score=110, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010499
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR020449
- InterPro:   IPR018060
- InterPro:   IPR011256 [H]

Pfam domain/function: PF06445 AraC_E_bind; PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 13819; Mature: 13819

Theoretical pI: Translated: 11.06; Mature: 11.06

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTGAIIQDLIDWIDNHLDSRLDIDTVARRAGYSKWHLQRIFKEHTGQPLGEYIRAKKLQ
CCCHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHH
KSIERLAHSNEPILNVAIALGFDSQQSFNRSFKRQYGQAPGVWRRSISRSVAQTSRQRSA
HHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNTGAIIQDLIDWIDNHLDSRLDIDTVARRAGYSKWHLQRIFKEHTGQPLGEYIRAKKLQ
CCCHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHH
KSIERLAHSNEPILNVAIALGFDSQQSFNRSFKRQYGQAPGVWRRSISRSVAQTSRQRSA
HHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]