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The map label for this gene is grpE [H]

Identifier: 238896059

GI number: 238896059

Start: 4026980

End: 4027570

Strand: Reverse

Name: grpE [H]

Synonym: KP1_4192

Alternate gene names: 238896059

Gene position: 4027570-4026980 (Counterclockwise)

Preceding gene: 238896063

Following gene: 238896056

Centisome position: 76.74

GC content: 56.01

Gene sequence:

>591_bases
ATGAGTAGTAAAGAACAGAAAACGCCTGAGGGGCAAGCCCCGGAAGAAATTATCACGGAGCAGCACGACGACGTTGAGGC
GGTAGAGCCTGAAGTTTCGGCTGAGCAGGTGGATCCGCGCGATGAAAAAATTGCGAATCTGGAAGCCCAGCTGGCTGAAG
CGCAGAAACGTGAACGTGAAGTGATGCTGCGTGCCAAAGCCGACGAAGATAACCTGCGTCGTCGTACCGAGCAGGATATC
GAGAAAGCGCACAAATTCGCGCTGGAAAAATTCGTCAATGAACTGCTGCCGGTAATCGACAGCCTGGACCGCGCGCTGGA
AGTGGCTGACAAGGCTAACCCGGAGCTGGCGCCGATGGTGGAGGGGATCGAACTGACCCTGAAATCTATGCTGGACGTAG
TGCGTAAGTTCGGCGTGGAAGTGATCGCCGACACCAACGTTCCGCTGGATCCGAACGTCCACCAGGCCATTGCGATGGTC
GAGTCTGAAGATGTGGCGGCGGGCAACGTGCTGTCCGTGATGCAGAAAGGTTATACCCTGAACGGCCGTACTATTCGCGC
CGCGATGGTGACGGTAGCGAAAGCGAAATAA

Upstream 100 bases:

>100_bases
GAAATAGTGACAATCTGTCCGCTTCCCTTGAAACCCGGAAACTGATCCCCATAATAAGCGAAGTTAGCGAGATGAATGCG
AAAAAAACGCGGAGAAATTC

Downstream 100 bases:

>100_bases
CGCTTGCTGCCTTGCTCAACGGCTGCCTGCGGGCGGCCGTTTTTTGTGTCATCGCTGCTGGCGCGAGATAAAAAAACGGC
GGGCCGCAGCCCACCGTGAT

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 196; Mature: 195

Protein sequence:

>196_residues
MSSKEQKTPEGQAPEEIITEQHDDVEAVEPEVSAEQVDPRDEKIANLEAQLAEAQKREREVMLRAKADEDNLRRRTEQDI
EKAHKFALEKFVNELLPVIDSLDRALEVADKANPELAPMVEGIELTLKSMLDVVRKFGVEVIADTNVPLDPNVHQAIAMV
ESEDVAAGNVLSVMQKGYTLNGRTIRAAMVTVAKAK

Sequences:

>Translated_196_residues
MSSKEQKTPEGQAPEEIITEQHDDVEAVEPEVSAEQVDPRDEKIANLEAQLAEAQKREREVMLRAKADEDNLRRRTEQDI
EKAHKFALEKFVNELLPVIDSLDRALEVADKANPELAPMVEGIELTLKSMLDVVRKFGVEVIADTNVPLDPNVHQAIAMV
ESEDVAAGNVLSVMQKGYTLNGRTIRAAMVTVAKAK
>Mature_195_residues
SSKEQKTPEGQAPEEIITEQHDDVEAVEPEVSAEQVDPRDEKIANLEAQLAEAQKREREVMLRAKADEDNLRRRTEQDIE
KAHKFALEKFVNELLPVIDSLDRALEVADKANPELAPMVEGIELTLKSMLDVVRKFGVEVIADTNVPLDPNVHQAIAMVE
SEDVAAGNVLSVMQKGYTLNGRTIRAAMVTVAKAK

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

Organism=Homo sapiens, GI24308295, Length=181, Percent_Identity=32.0441988950276, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI40255109, Length=137, Percent_Identity=35.7664233576642, Blast_Score=74, Evalue=6e-14,
Organism=Escherichia coli, GI1788967, Length=196, Percent_Identity=86.734693877551, Blast_Score=345, Evalue=1e-96,
Organism=Caenorhabditis elegans, GI17552458, Length=139, Percent_Identity=33.0935251798561, Blast_Score=67, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6324806, Length=180, Percent_Identity=32.7777777777778, Blast_Score=95, Evalue=9e-21,
Organism=Drosophila melanogaster, GI24653432, Length=175, Percent_Identity=32.5714285714286, Blast_Score=89, Evalue=2e-18,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 21751; Mature: 21620

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSKEQKTPEGQAPEEIITEQHDDVEAVEPEVSAEQVDPRDEKIANLEAQLAEAQKRERE
CCCCCCCCCCCCCCHHHHHCCCCCHHHCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHH
VMLRAKADEDNLRRRTEQDIEKAHKFALEKFVNELLPVIDSLDRALEVADKANPELAPMV
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
EGIELTLKSMLDVVRKFGVEVIADTNVPLDPNVHQAIAMVESEDVAAGNVLSVMQKGYTL
HHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCC
NGRTIRAAMVTVAKAK
CCCHHHHHHHHHHCCC
>Mature Secondary Structure 
SSKEQKTPEGQAPEEIITEQHDDVEAVEPEVSAEQVDPRDEKIANLEAQLAEAQKRERE
CCCCCCCCCCCCCHHHHHCCCCCHHHCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHH
VMLRAKADEDNLRRRTEQDIEKAHKFALEKFVNELLPVIDSLDRALEVADKANPELAPMV
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
EGIELTLKSMLDVVRKFGVEVIADTNVPLDPNVHQAIAMVESEDVAAGNVLSVMQKGYTL
HHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCC
NGRTIRAAMVTVAKAK
CCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA