Click here to switch to the map view.

The map label for this gene is yfhD

Identifier: 238896009

GI number: 238896009

Start: 3972422

End: 3973957

Strand: Direct

Name: yfhD

Synonym: KP1_4137

Alternate gene names: 238896009

Gene position: 3972422-3973957 (Clockwise)

Preceding gene: 238896003

Following gene: 238896012

Centisome position: 75.69

GC content: 57.29

Gene sequence:

>1536_bases
CTGAAAAAATTAAAGATTAATTATCTGCTGATCGGCATTGTCACCCTTTTGCTGGCGGCGGCCCTGTGGCCGTCGATCCC
CTGGTCTGGTAAACCCGAGAACCGGGTGGCGGGCATTATCGCTCGCGGCGAGCTGCGCATCAGCACTATCAACTCGCCAA
TGACCTTCGCCACCATGAACAATAAAGCGTTCGGTCTGGATTATGAACTGGCGAAGCAGTTTGCCGACTACCTTGGCGTA
ACGCTTAAGATAACCGTCCGGCAAAACATCAGCCAGCTGTTTGACGACCTCGACGACGGTCAGGCCGATATGCTGGCCGC
CGGACTGGTCTATAACCAGGAGCGAGTGAAAAACTATCAGGCCGGCCCCACCTACTATTCTGTTTCGCAACAGCTGGTCT
ACCGCGTCGGCAATACCCGGCCGCGCACCCTCGCGGCGCTGACCGCCGAGCAGCTCACTATCGCTCCGGGCCACGTGGCG
ATCAACGACCTGCAGACCTTAAAAGCCGAGAAATACCCGGATCTTGCCTGGCGCGTGGACGAAAAACGCGGTACCACGGC
GCTGATGCAGGCGGTGATTGACGGTAAACTTGATTACACCATCGCTGACTCGGTGGCCGTCAGCCTGTTTCAGCGCGTCC
ATCCGGAGCTGGCGGTGGCCCTCGATATCACTGACGAACAGCCGGTCACCTGGTTTAGCGCCCGGGACGACGATAATTCG
CTGTCGGCGGCAATGCTTGATTTCTTCAACAACATCAATGAAGACGGCACCCTGGCGCGCCTGGAGGAGAAATACCTCGG
CCACGGCAACGATTTCGATTATGTCGATACCCGCACCTTCCTGCGCGCGGTGGAAAATATTCTTCCGGAGGTGCAGCCGC
TGTTTGAAAAATACGCCCGCGAGATTGACTGGCGCCTGCTGGCCGCCATCGCCTGGCAGGAATCCCACTGGGATCCGCAG
GCCACCTCCCCCACCGGCGTGCGCGGTATGATGATGCTCACACGCAATACGGCGCAAAGCCTGGGTCTAACCGACCGCAC
CGATGCGGCGCAGAGTATCGACGGCGGCATGCGCTACCTGCAGGATATGATGGATAAAGTGCCGGATTCGATCCCGAAAG
ATGAACGCATCTGGTTTGCGCTGGCCGCTTATAATATGGGCTATGCCCATATGCTGGACGCCATGGCGCTCACCCGCAAA
CAGAAAGGCAATCCTAACAGCTGGGCGGACGTTAAGCTGCGTTTACCGTTGTTGAGCCAGAAACCCTATTACAGCAAACT
AAAATACGGCTATGCCCGCGGCCATGAGGCCTACGCCTATGTGGAAAACATCCGCAAATATCAGATAAGCCTTGTCGGCT
ATTTGTCAGAGAAGGAGCGCCAGCAGCAGCAAACGCTGGCCCTGGCGGAGGATTACCCGGCCGTGCTGCCCAATGAGCTC
GAACAGCCGCAGGAGACCACCCTGCCCTTTTTCAAGTTTCGCGCCGATAAGCAGATGGATAATGCCCGGATGAAACTCCC
CGGCCACCTGTATTAA

Upstream 100 bases:

>100_bases
TGGCGCATTATTAAGCAGAATGCCGCACATTCGACGACAACCTTGCGTTTTTTCCGGACATCGCCTGCTGAGGAACCTGA
AGCTGCAGAAGATTAACTCT

Downstream 100 bases:

>100_bases
CTTTCCCCGGTCTGCGCCCGGGCTTTCTTCAGCGCTTTTTTCTCTTCCCGCCGCCAGCGGAAGAAGTCGCTCAGCATGGC
AGAGCAGGACTCTGCCAGCA

Product: putative transglycosylase

Products: NA

Alternate protein names: Murein lyase F

Number of amino acids: Translated: 511; Mature: 511

Protein sequence:

>511_residues
MKKLKINYLLIGIVTLLLAAALWPSIPWSGKPENRVAGIIARGELRISTINSPMTFATMNNKAFGLDYELAKQFADYLGV
TLKITVRQNISQLFDDLDDGQADMLAAGLVYNQERVKNYQAGPTYYSVSQQLVYRVGNTRPRTLAALTAEQLTIAPGHVA
INDLQTLKAEKYPDLAWRVDEKRGTTALMQAVIDGKLDYTIADSVAVSLFQRVHPELAVALDITDEQPVTWFSARDDDNS
LSAAMLDFFNNINEDGTLARLEEKYLGHGNDFDYVDTRTFLRAVENILPEVQPLFEKYAREIDWRLLAAIAWQESHWDPQ
ATSPTGVRGMMMLTRNTAQSLGLTDRTDAAQSIDGGMRYLQDMMDKVPDSIPKDERIWFALAAYNMGYAHMLDAMALTRK
QKGNPNSWADVKLRLPLLSQKPYYSKLKYGYARGHEAYAYVENIRKYQISLVGYLSEKERQQQQTLALAEDYPAVLPNEL
EQPQETTLPFFKFRADKQMDNARMKLPGHLY

Sequences:

>Translated_511_residues
MKKLKINYLLIGIVTLLLAAALWPSIPWSGKPENRVAGIIARGELRISTINSPMTFATMNNKAFGLDYELAKQFADYLGV
TLKITVRQNISQLFDDLDDGQADMLAAGLVYNQERVKNYQAGPTYYSVSQQLVYRVGNTRPRTLAALTAEQLTIAPGHVA
INDLQTLKAEKYPDLAWRVDEKRGTTALMQAVIDGKLDYTIADSVAVSLFQRVHPELAVALDITDEQPVTWFSARDDDNS
LSAAMLDFFNNINEDGTLARLEEKYLGHGNDFDYVDTRTFLRAVENILPEVQPLFEKYAREIDWRLLAAIAWQESHWDPQ
ATSPTGVRGMMMLTRNTAQSLGLTDRTDAAQSIDGGMRYLQDMMDKVPDSIPKDERIWFALAAYNMGYAHMLDAMALTRK
QKGNPNSWADVKLRLPLLSQKPYYSKLKYGYARGHEAYAYVENIRKYQISLVGYLSEKERQQQQTLALAEDYPAVLPNEL
EQPQETTLPFFKFRADKQMDNARMKLPGHLY
>Mature_511_residues
MKKLKINYLLIGIVTLLLAAALWPSIPWSGKPENRVAGIIARGELRISTINSPMTFATMNNKAFGLDYELAKQFADYLGV
TLKITVRQNISQLFDDLDDGQADMLAAGLVYNQERVKNYQAGPTYYSVSQQLVYRVGNTRPRTLAALTAEQLTIAPGHVA
INDLQTLKAEKYPDLAWRVDEKRGTTALMQAVIDGKLDYTIADSVAVSLFQRVHPELAVALDITDEQPVTWFSARDDDNS
LSAAMLDFFNNINEDGTLARLEEKYLGHGNDFDYVDTRTFLRAVENILPEVQPLFEKYAREIDWRLLAAIAWQESHWDPQ
ATSPTGVRGMMMLTRNTAQSLGLTDRTDAAQSIDGGMRYLQDMMDKVPDSIPKDERIWFALAAYNMGYAHMLDAMALTRK
QKGNPNSWADVKLRLPLLSQKPYYSKLKYGYARGHEAYAYVENIRKYQISLVGYLSEKERQQQQTLALAEDYPAVLPNEL
EQPQETTLPFFKFRADKQMDNARMKLPGHLY

Specific function: Murein-degrading enzyme that degrades murein glycan strands and insoluble, high-molecular weight murein sacculi, with the concomitant formation of a 1,6-anhydromuramoyl product. Lytic transglycosylases (LTs) play an integral role in the metabolism of the

COG id: COG4623

COG function: function code M; Predicted soluble lytic transglycosylase fused to an ABC-type amino acid-binding protein

Gene ontology:

Cell location: Cell outer membrane; Peripheral membrane protein. Note=Attached to the inner leaflet of the outer membrane

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transglycosylase slt family

Homologues:

Organism=Escherichia coli, GI171474010, Length=481, Percent_Identity=80.04158004158, Blast_Score=801, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MLTF_KLEP7 (A6TCH2)

Other databases:

- EMBL:   CP000647
- RefSeq:   YP_001336523.1
- ProteinModelPortal:   A6TCH2
- STRING:   A6TCH2
- GeneID:   5338602
- GenomeReviews:   CP000647_GR
- KEGG:   kpn:KPN_02883
- eggNOG:   COG4623
- HOGENOM:   HBG644469
- ProtClustDB:   PRK10859
- HAMAP:   MF_02016
- InterPro:   IPR008258
- InterPro:   IPR001638
- InterPro:   IPR000189
- SMART:   SM00062

Pfam domain/function: PF00497 SBP_bac_3; PF01464 SLT

EC number: NA

Molecular weight: Translated: 57909; Mature: 57909

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: ACT_SITE 314-314

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLKINYLLIGIVTLLLAAALWPSIPWSGKPENRVAGIIARGELRISTINSPMTFATMN
CCEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECEEEEEECCCCEEEEEEC
NKAFGLDYELAKQFADYLGVTLKITVRQNISQLFDDLDDGQADMLAAGLVYNQERVKNYQ
CCEECCCHHHHHHHHHHHCEEEEEEEHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHCCC
AGPTYYSVSQQLVYRVGNTRPRTLAALTAEQLTIAPGHVAINDLQTLKAEKYPDLAWRVD
CCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHEEECCCCEEHHHHHHHHHCCCCCCEEEEC
EKRGTTALMQAVIDGKLDYTIADSVAVSLFQRVHPELAVALDITDEQPVTWFSARDDDNS
CCCCHHHHHHHHHCCCCCEEHHHHHHHHHHHHHCCCEEEEEEECCCCCEEEEECCCCCCH
LSAAMLDFFNNINEDGTLARLEEKYLGHGNDFDYVDTRTFLRAVENILPEVQPLFEKYAR
HHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
EIDWRLLAAIAWQESHWDPQATSPTGVRGMMMLTRNTAQSLGLTDRTDAAQSIDGGMRYL
HHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEHHHHHHCCCCCCCHHHHHHHHHHHHH
QDMMDKVPDSIPKDERIWFALAAYNMGYAHMLDAMALTRKQKGNPNSWADVKLRLPLLSQ
HHHHHHCCCCCCCCCCEEEEEEHHCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCC
KPYYSKLKYGYARGHEAYAYVENIRKYQISLVGYLSEKERQQQQTLALAEDYPAVLPNEL
CCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
EQPQETTLPFFKFRADKQMDNARMKLPGHLY
CCCHHHCCCHHEEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKKLKINYLLIGIVTLLLAAALWPSIPWSGKPENRVAGIIARGELRISTINSPMTFATMN
CCEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECEEEEEECCCCEEEEEEC
NKAFGLDYELAKQFADYLGVTLKITVRQNISQLFDDLDDGQADMLAAGLVYNQERVKNYQ
CCEECCCHHHHHHHHHHHCEEEEEEEHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHCCC
AGPTYYSVSQQLVYRVGNTRPRTLAALTAEQLTIAPGHVAINDLQTLKAEKYPDLAWRVD
CCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHEEECCCCEEHHHHHHHHHCCCCCCEEEEC
EKRGTTALMQAVIDGKLDYTIADSVAVSLFQRVHPELAVALDITDEQPVTWFSARDDDNS
CCCCHHHHHHHHHCCCCCEEHHHHHHHHHHHHHCCCEEEEEEECCCCCEEEEECCCCCCH
LSAAMLDFFNNINEDGTLARLEEKYLGHGNDFDYVDTRTFLRAVENILPEVQPLFEKYAR
HHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
EIDWRLLAAIAWQESHWDPQATSPTGVRGMMMLTRNTAQSLGLTDRTDAAQSIDGGMRYL
HHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEHHHHHHCCCCCCCHHHHHHHHHHHHH
QDMMDKVPDSIPKDERIWFALAAYNMGYAHMLDAMALTRKQKGNPNSWADVKLRLPLLSQ
HHHHHHCCCCCCCCCCEEEEEEHHCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCC
KPYYSKLKYGYARGHEAYAYVENIRKYQISLVGYLSEKERQQQQTLALAEDYPAVLPNEL
CCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
EQPQETTLPFFKFRADKQMDNARMKLPGHLY
CCCHHHCCCHHEEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA