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The map label for this gene is hmpA [H]

Identifier: 238896003

GI number: 238896003

Start: 3962557

End: 3963747

Strand: Direct

Name: hmpA [H]

Synonym: KP1_4131

Alternate gene names: 238896003

Gene position: 3962557-3963747 (Clockwise)

Preceding gene: 238896001

Following gene: 238896009

Centisome position: 75.5

GC content: 57.18

Gene sequence:

>1191_bases
ATGCTTGACGCTCAAACCATCGCCACGGTAAAAGCCACCATTCCCCTGCTGGTAGAGACCGGCCCGAAATTAACCGCCCA
TTTCTACGATCGTATGTTCGCGCATAATCCGGAGCTCAAAGAGATCTTCAACATGAGCAACCAGCGCAACGGCGATCAGC
GTGAAGCGTTATTCAACGCTATTGCCGCCTACGCCAGCAATATCGATAACCTGCCTGCCCTGCTGCCGGCGGTGGAAAAG
ATCGCCCAGAAACACACCAGCTTCCAGATCAAACCGGAGCAGTACAATATTGTCGGCAGCCATCTGCTGGCGACCCTCGA
TGAAATGTTCAGCCCGGGCCAGGAAGTGCTCGATGCCTGGGGTAAAGCCTACGGCGTGCTGGCGAACGTCTTTATCGGCC
GTGAAGCCGAGATCTATCAGCAAAACGCCAGTAAAACCGGCGGCTGGGAAGGGACTCGCGCGTTCCGCATCGTGAAGAAA
ACCCCGCGCAGCCAGCTTATCATCAGCTTTGAGCTCGAACCGGTTGATGGCCAGCCGGTCGCCGATTACCAGCCTGGCCA
GTACCTGGCCATCTGGCTGAAACCGGAAGGCTTCGAATATCAGGAAATCCGCCAGTACTCCCTGACCCGCAAAGCGGACG
GCAAAGGCTACCGCATTGCGGTTAAACGCGAGGAAGGCGGTCAGGTTTCCAGCTGGCTGCATAACCATGCCAGCGAAGGC
GATGTGGTCTATCTGGCCGCCCCGGCGGGAGATTTCTTCCTTAACGTTAAGCCACAGACGCCGGTCACTCTGCTCTCTGG
CGGCGTCGGGCAAACGCCGATGCTGGCGATGCTGGATGCGCTGGCGAAGAGCGGTCATCAGGGTCAGGTGAACTGGTTCC
ATGCGGCGGAAAATGGCGACGTTCACGCCTTTGCCGATGAAGTGAAGGCGCTCGGCGCGGCGCTGCCGGCGTTTACCTCT
CACGTCTGGTATCGCACCCCGACTGAGGCCGACCGTCAGGCTGGACGTTTTGATAGCGAAGGTTTGATGGATTTAGCAGC
AGTGGCGGACAATATTCGCGACCCGCAGATGCAGTATTATCTCTGCGGCCCGGTAGCGTTTATGCAGTTTGCAGCGAAGC
AGCTGGTCGAATTAGGGATCAACAAAGACAACATTCATTACGAATGCTTCGGCCCGCATAAGGTGCTGTAA

Upstream 100 bases:

>100_bases
GGATCGGGGGAATGTGTTAAAGAACCATTTACAACGCAGGGTTATTTCTTTAAGATGCATTTAAAATGCATTATTAAGAT
AACACATAAGGAAGCACGCT

Downstream 100 bases:

>100_bases
GCCCCGACGATGCGGGGATCTGTAGGCCGGGTAAGCACAGCGCCACCCGGCAATACTGCAGCGAGCGGCGAGCGTTAAAT
CGCCGCGTCGTCTTCTTCGC

Product: nitric oxide dioxygenase

Products: NA

Alternate protein names: Flavohemoglobin; Hemoglobin-like protein; Nitric oxide dioxygenase; NO oxygenase; NOD [H]

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFAHNPELKEIFNMSNQRNGDQREALFNAIAAYASNIDNLPALLPAVEK
IAQKHTSFQIKPEQYNIVGSHLLATLDEMFSPGQEVLDAWGKAYGVLANVFIGREAEIYQQNASKTGGWEGTRAFRIVKK
TPRSQLIISFELEPVDGQPVADYQPGQYLAIWLKPEGFEYQEIRQYSLTRKADGKGYRIAVKREEGGQVSSWLHNHASEG
DVVYLAAPAGDFFLNVKPQTPVTLLSGGVGQTPMLAMLDALAKSGHQGQVNWFHAAENGDVHAFADEVKALGAALPAFTS
HVWYRTPTEADRQAGRFDSEGLMDLAAVADNIRDPQMQYYLCGPVAFMQFAAKQLVELGINKDNIHYECFGPHKVL

Sequences:

>Translated_396_residues
MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFAHNPELKEIFNMSNQRNGDQREALFNAIAAYASNIDNLPALLPAVEK
IAQKHTSFQIKPEQYNIVGSHLLATLDEMFSPGQEVLDAWGKAYGVLANVFIGREAEIYQQNASKTGGWEGTRAFRIVKK
TPRSQLIISFELEPVDGQPVADYQPGQYLAIWLKPEGFEYQEIRQYSLTRKADGKGYRIAVKREEGGQVSSWLHNHASEG
DVVYLAAPAGDFFLNVKPQTPVTLLSGGVGQTPMLAMLDALAKSGHQGQVNWFHAAENGDVHAFADEVKALGAALPAFTS
HVWYRTPTEADRQAGRFDSEGLMDLAAVADNIRDPQMQYYLCGPVAFMQFAAKQLVELGINKDNIHYECFGPHKVL
>Mature_396_residues
MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFAHNPELKEIFNMSNQRNGDQREALFNAIAAYASNIDNLPALLPAVEK
IAQKHTSFQIKPEQYNIVGSHLLATLDEMFSPGQEVLDAWGKAYGVLANVFIGREAEIYQQNASKTGGWEGTRAFRIVKK
TPRSQLIISFELEPVDGQPVADYQPGQYLAIWLKPEGFEYQEIRQYSLTRKADGKGYRIAVKREEGGQVSSWLHNHASEG
DVVYLAAPAGDFFLNVKPQTPVTLLSGGVGQTPMLAMLDALAKSGHQGQVNWFHAAENGDVHAFADEVKALGAALPAFTS
HVWYRTPTEADRQAGRFDSEGLMDLAAVADNIRDPQMQYYLCGPVAFMQFAAKQLVELGINKDNIHYECFGPHKVL

Specific function: Is involved in NO detoxification in an aerobic process, termed nitric oxide dioxygenase (NOD) reaction that utilizes O(2) and NAD(P)H to convert NO to nitrate, which protects the bacterium from various noxious nitrogen compounds. Therefore, plays a centra

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1788903, Length=396, Percent_Identity=83.8383838383838, Blast_Score=702, Evalue=0.0,
Organism=Escherichia coli, GI1787658, Length=239, Percent_Identity=26.7782426778243, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1788104, Length=234, Percent_Identity=25.2136752136752, Blast_Score=64, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6321673, Length=411, Percent_Identity=34.7931873479319, Blast_Score=214, Evalue=2e-56,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017927
- InterPro:   IPR012292
- InterPro:   IPR009050
- InterPro:   IPR000971
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR001221
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00042 Globin; PF00175 NAD_binding_1 [H]

EC number: =1.14.12.17 [H]

Molecular weight: Translated: 43841; Mature: 43841

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS01033 GLOBIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFAHNPELKEIFNMSNQRNGDQREALFNA
CCCCHHHHHHHHEEEEEEECCCCHHHHHHHHHHHCCCCHHHHHCCCCCCCCHHHHHHHHH
IAAYASNIDNLPALLPAVEKIAQKHTSFQIKPEQYNIVGSHLLATLDEMFSPGQEVLDAW
HHHHHCCCCCCHHHHHHHHHHHHHHCCEEECCHHHHHHHHHHHHHHHHHHCCHHHHHHHH
GKAYGVLANVFIGREAEIYQQNASKTGGWEGTRAFRIVKKTPRSQLIISFELEPVDGQPV
HHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCHHEEEEECCCCCCEEEEEEEEECCCCCCC
ADYQPGQYLAIWLKPEGFEYQEIRQYSLTRKADGKGYRIAVKREEGGQVSSWLHNHASEG
CCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHCCCCC
DVVYLAAPAGDFFLNVKPQTPVTLLSGGVGQTPMLAMLDALAKSGHQGQVNWFHAAENGD
CEEEEEECCCCEEEEECCCCCEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCC
VHAFADEVKALGAALPAFTSHVWYRTPTEADRQAGRFDSEGLMDLAAVADNIRDPQMQYY
CHHHHHHHHHHHHHHHHHHHCCEEECCCHHHHHCCCCCCCHHHHHHHHHHCCCCCCEEEE
LCGPVAFMQFAAKQLVELGINKDNIHYECFGPHKVL
EECHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCC
>Mature Secondary Structure
MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFAHNPELKEIFNMSNQRNGDQREALFNA
CCCCHHHHHHHHEEEEEEECCCCHHHHHHHHHHHCCCCHHHHHCCCCCCCCHHHHHHHHH
IAAYASNIDNLPALLPAVEKIAQKHTSFQIKPEQYNIVGSHLLATLDEMFSPGQEVLDAW
HHHHHCCCCCCHHHHHHHHHHHHHHCCEEECCHHHHHHHHHHHHHHHHHHCCHHHHHHHH
GKAYGVLANVFIGREAEIYQQNASKTGGWEGTRAFRIVKKTPRSQLIISFELEPVDGQPV
HHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCHHEEEEECCCCCCEEEEEEEEECCCCCCC
ADYQPGQYLAIWLKPEGFEYQEIRQYSLTRKADGKGYRIAVKREEGGQVSSWLHNHASEG
CCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHCCCCC
DVVYLAAPAGDFFLNVKPQTPVTLLSGGVGQTPMLAMLDALAKSGHQGQVNWFHAAENGD
CEEEEEECCCCEEEEECCCCCEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCC
VHAFADEVKALGAALPAFTSHVWYRTPTEADRQAGRFDSEGLMDLAAVADNIRDPQMQYY
CHHHHHHHHHHHHHHHHHHHCCEEECCCHHHHHCCCCCCCHHHHHHHHHHCCCCCCEEEE
LCGPVAFMQFAAKQLVELGINKDNIHYECFGPHKVL
EECHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]