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The map label for this gene is yfgB [H]

Identifier: 238895973

GI number: 238895973

Start: 3926818

End: 3927984

Strand: Reverse

Name: yfgB [H]

Synonym: KP1_4098

Alternate gene names: 238895973

Gene position: 3927984-3926818 (Counterclockwise)

Preceding gene: 238895974

Following gene: 238895972

Centisome position: 74.84

GC content: 57.67

Gene sequence:

>1167_bases
ATGTCTGAACAAATTGTCACGCCTGATACCGCCGCTCTGACGGTGCCGAACAAAGATGCCAAAATCAACCTGCTGGATTT
AAACCGTCAGCAGATGCGCGAGTTTTTCAAAAACATGGGCGAGAAGCCTTTCCGCGCCGATCAGGTGATGAAATGGATGT
ATCACTACTGCTGCGACGACTTTGATGAGATGACCGACATCAACAAAGTCCTGCGCAGTAAACTGAAAGAAGTGGCGGAG
ATCCGCGCCCCGGAAGTGGTTGAAGAGCAACGCTCAACCGACGGGACCATTAAATGGGCGATTGCCGTTGGCGATCAGCG
CGTCGAGACCGTCTATATCCCGGAAGAAGACCGCGCCACCCTGTGCGTCTCCTCCCAGGTAGGCTGCGCGCTGGAGTGCA
AATTCTGCTCCACCGCCCAGCAGGGCTTTAACCGCAACCTGCGGGTATCCGAAATTATCGGCCAGGTCTGGCGCGCGGCG
AAGATCGTCGGCGCGGTGAAGACCACCGGCGTGCGTCCCATTACCAACGTAGTAATGATGGGCATGGGCGAGCCGCTGCT
GAACCTCAACAACGTCGTTCCGGCGATGGAAATCATGCTCGACGATTTCGGCTTTGGCCTTTCCAAGCGTCGTGTAACCT
TGTCTACCTCCGGCGTGGTGCCGGCGCTGGATAAACTCGGCGATATGATCGACGTTGCGCTGGCGATCTCCTTGCACGCG
CCGAACGACACCATTCGTGACGAAATCGTCCCGATCAACAAAAAGTACAACATCGAGACGTTCCTCAACTCGGTGCGCGG
CTATATTTCGAAGTCCAACGCCAACCAGGGCCGGGTGACCATCGAGTACGTGATGCTGGATCACGTCAACGACGGCACCG
AGCACGCCCATGAGCTGGCGGCGCTGCTGAAAGATACGCCGTGCAAGATCAACCTGATCCCATGGAACCCGTTCCCGGGC
GCGCCGTATGGCCGCAGCTCCAACAGCCGCATCGACCGTTTCTCCAAAGTCCTGATGGAGTACGGCTTTACGACCATCGT
GCGTAAAACGCGCGGCGACGATATCGATGCCGCCTGCGGCCAGCTGGCGGGCGACGTCATTGACCGTACCAAGCGTACGC
TGCGTAAGCGGATGCAGGGCGAAGCGATTGACGTAAAAGCGGTCTGA

Upstream 100 bases:

>100_bases
TGTAGAATATAGCGCCCCTGGACGAGCAGTCGCTCTCCGGGGCGTTTCTTTTTCAACCCACCTGGGGCCATAACGTGTAA
TAACGAGGCCGGAAAATATT

Downstream 100 bases:

>100_bases
CAAATGATGACCCGTGCAGCGCAGGCGGCACGGGTCATATTTATGGCCGATAGCCAGGCGAAATACGCTGATTCGGTTAA
TAATTACGGTGCGTTTTTGT

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase [H]

Number of amino acids: Translated: 388; Mature: 387

Protein sequence:

>388_residues
MSEQIVTPDTAALTVPNKDAKINLLDLNRQQMREFFKNMGEKPFRADQVMKWMYHYCCDDFDEMTDINKVLRSKLKEVAE
IRAPEVVEEQRSTDGTIKWAIAVGDQRVETVYIPEEDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAA
KIVGAVKTTGVRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPALDKLGDMIDVALAISLHA
PNDTIRDEIVPINKKYNIETFLNSVRGYISKSNANQGRVTIEYVMLDHVNDGTEHAHELAALLKDTPCKINLIPWNPFPG
APYGRSSNSRIDRFSKVLMEYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDVKAV

Sequences:

>Translated_388_residues
MSEQIVTPDTAALTVPNKDAKINLLDLNRQQMREFFKNMGEKPFRADQVMKWMYHYCCDDFDEMTDINKVLRSKLKEVAE
IRAPEVVEEQRSTDGTIKWAIAVGDQRVETVYIPEEDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAA
KIVGAVKTTGVRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPALDKLGDMIDVALAISLHA
PNDTIRDEIVPINKKYNIETFLNSVRGYISKSNANQGRVTIEYVMLDHVNDGTEHAHELAALLKDTPCKINLIPWNPFPG
APYGRSSNSRIDRFSKVLMEYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDVKAV
>Mature_387_residues
SEQIVTPDTAALTVPNKDAKINLLDLNRQQMREFFKNMGEKPFRADQVMKWMYHYCCDDFDEMTDINKVLRSKLKEVAEI
RAPEVVEEQRSTDGTIKWAIAVGDQRVETVYIPEEDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAK
IVGAVKTTGVRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPALDKLGDMIDVALAISLHAP
NDTIRDEIVPINKKYNIETFLNSVRGYISKSNANQGRVTIEYVMLDHVNDGTEHAHELAALLKDTPCKINLIPWNPFPGA
PYGRSSNSRIDRFSKVLMEYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDVKAV

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family [H]

Homologues:

Organism=Escherichia coli, GI1788865, Length=388, Percent_Identity=92.5257731958763, Blast_Score=749, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.1.1.192 [H]

Molecular weight: Translated: 43376; Mature: 43245

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQIVTPDTAALTVPNKDAKINLLDLNRQQMREFFKNMGEKPFRADQVMKWMYHYCCDD
CCCCCCCCCCCEEEECCCCCEEEEEECCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCC
FDEMTDINKVLRSKLKEVAEIRAPEVVEEQRSTDGTIKWAIAVGDQRVETVYIPEEDRAT
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCEEEEEEEECCCCEEEEECCCCCCCE
LCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVGAVKTTGVRPITNVVMM
EEEECCCCCEEEEHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
GMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPALDKLGDMIDVALAISLHA
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHEEHHEEEEEC
PNDTIRDEIVPINKKYNIETFLNSVRGYISKSNANQGRVTIEYVMLDHVNDGTEHAHELA
CCCCHHHCEEECCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEEEEECCCCCHHHHHHHH
ALLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVLMEYGFTTIVRKTRGDDIDAACG
HHHCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHCCCCCHHHHHH
QLAGDVIDRTKRTLRKRMQGEAIDVKAV
HHHHHHHHHHHHHHHHHHCCCCEEEEEC
>Mature Secondary Structure 
SEQIVTPDTAALTVPNKDAKINLLDLNRQQMREFFKNMGEKPFRADQVMKWMYHYCCDD
CCCCCCCCCCEEEECCCCCEEEEEECCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCC
FDEMTDINKVLRSKLKEVAEIRAPEVVEEQRSTDGTIKWAIAVGDQRVETVYIPEEDRAT
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCEEEEEEEECCCCEEEEECCCCCCCE
LCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVGAVKTTGVRPITNVVMM
EEEECCCCCEEEEHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
GMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPALDKLGDMIDVALAISLHA
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHEEHHEEEEEC
PNDTIRDEIVPINKKYNIETFLNSVRGYISKSNANQGRVTIEYVMLDHVNDGTEHAHELA
CCCCHHHCEEECCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEEEEECCCCCHHHHHHHH
ALLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVLMEYGFTTIVRKTRGDDIDAACG
HHHCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHCCCCCHHHHHH
QLAGDVIDRTKRTLRKRMQGEAIDVKAV
HHHHHHHHHHHHHHHHHHCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA