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The map label for this gene is dapE [H]

Identifier: 238895931

GI number: 238895931

Start: 3880401

End: 3881528

Strand: Direct

Name: dapE [H]

Synonym: KP1_4056

Alternate gene names: 238895931

Gene position: 3880401-3881528 (Clockwise)

Preceding gene: 238895930

Following gene: 238895932

Centisome position: 73.93

GC content: 60.28

Gene sequence:

>1128_bases
ATGTCTTGCCCGGTCATTGAGCTGGCTCAGCAGCTTATTCGCCGCCCTTCCCTTAGCCCCGATGATGCGGGATGCCAGGC
GTTAATGATTGAACGCCTGCGTGCGATCGGCTTTACCATTGAACCGATGGATTTCGGCGATACGCAGAATTTCTGGGCCT
GGCGCGGCCACGGCGAGACGCTGGCCTTCGCCGGCCACACCGACGTAGTCCCGGCAGGCGACGCCGACCGCTGGATCAAT
CCCCCGTTCGAACCGACCATCCGCGACGGGATGCTGTTTGGCCGCGGAGCGGCGGATATGAAAGGCTCGCTGGCGGCGAT
GGTGGTTGCCGCAGAGCGTTTTGTCGCGCAGTACCCGAACCACAGGGGCCGACTGGCGTTTTTGATCACCTCTGATGAAG
AGGCCAGCGCCAAAAACGGCACCGTCAAAGTGGTGGAGACGCTGATGGCGCGTAACGAGCGTCTGGACTATTGTCTGGTC
GGCGAACCTTCCAGTACCGAAGTGGTCGGCGATGTGGTGAAAAACGGCCGCCGCGGCTCGCTGACCTGCAACCTGACTAT
TCACGGCGTGCAGGGGCATGTCGCCTATCCGCACCTTGCCGATAACCCTGTCCACCGCGCGGCGCCGATGCTGGCGGAAC
TGGTCAATATCGAATGGGATAAAGGCAATGAGTTCTTCCCGCCGACCAGCATGCAGATCGCCAACGTGCAGTCCGGCACC
GGCAGCAATAACGTGATCCCCGGCGATATGTTCGTCCAGTTCAACTTCCGCTTCAGTACGGAGCTGACGGACGAGATGAT
CAAATCCCGGGTGATCGCCCTGCTGGAAAAATACCAGCTGCGTTACAGCGTGGAGTGGTGGCTCTCCGGCCAGCCGTTCC
TCACCGGACGGGGCAAGCTGGTGGATGCGGTCGTCAACGCGATTGAGCACTATAATGAGATCAAACCGCAGCTGTTAACC
AACGGCGGCACCTCAGACGGACGGTTTATCGCGCGCATGGGGGCGCAGGTGGTTGAGCTGGGCCCCGTAAACGCAACGAT
TCACAAAATTAATGAATGTGTGAACGCCGCCGACCTGCAGCTGCTGGCGCGGATGTATCAACGCGTCATGGAACAGCTGG
TCGCCTGA

Upstream 100 bases:

>100_bases
GCCGGCAATTATCAAACGCCCATTGCTCTGCGCGCCCGGTCGTCCTATGCTGCTGGGTTTTAGTGAAGCAAGTTATCAGC
AGTTTAACGAGGTATAGTCT

Downstream 100 bases:

>100_bases
CGAATTGACGTAAAGAGGAAGCGCGCATGGAATGGCTTATTAAACACTGGTGGATTCTGGTGCTGGTCTTTTTGGTGGGG
GTAATTATCAACGTGATTAA

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 375; Mature: 374

Protein sequence:

>375_residues
MSCPVIELAQQLIRRPSLSPDDAGCQALMIERLRAIGFTIEPMDFGDTQNFWAWRGHGETLAFAGHTDVVPAGDADRWIN
PPFEPTIRDGMLFGRGAADMKGSLAAMVVAAERFVAQYPNHRGRLAFLITSDEEASAKNGTVKVVETLMARNERLDYCLV
GEPSSTEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPMLAELVNIEWDKGNEFFPPTSMQIANVQSGT
GSNNVIPGDMFVQFNFRFSTELTDEMIKSRVIALLEKYQLRYSVEWWLSGQPFLTGRGKLVDAVVNAIEHYNEIKPQLLT
NGGTSDGRFIARMGAQVVELGPVNATIHKINECVNAADLQLLARMYQRVMEQLVA

Sequences:

>Translated_375_residues
MSCPVIELAQQLIRRPSLSPDDAGCQALMIERLRAIGFTIEPMDFGDTQNFWAWRGHGETLAFAGHTDVVPAGDADRWIN
PPFEPTIRDGMLFGRGAADMKGSLAAMVVAAERFVAQYPNHRGRLAFLITSDEEASAKNGTVKVVETLMARNERLDYCLV
GEPSSTEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPMLAELVNIEWDKGNEFFPPTSMQIANVQSGT
GSNNVIPGDMFVQFNFRFSTELTDEMIKSRVIALLEKYQLRYSVEWWLSGQPFLTGRGKLVDAVVNAIEHYNEIKPQLLT
NGGTSDGRFIARMGAQVVELGPVNATIHKINECVNAADLQLLARMYQRVMEQLVA
>Mature_374_residues
SCPVIELAQQLIRRPSLSPDDAGCQALMIERLRAIGFTIEPMDFGDTQNFWAWRGHGETLAFAGHTDVVPAGDADRWINP
PFEPTIRDGMLFGRGAADMKGSLAAMVVAAERFVAQYPNHRGRLAFLITSDEEASAKNGTVKVVETLMARNERLDYCLVG
EPSSTEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPMLAELVNIEWDKGNEFFPPTSMQIANVQSGTG
SNNVIPGDMFVQFNFRFSTELTDEMIKSRVIALLEKYQLRYSVEWWLSGQPFLTGRGKLVDAVVNAIEHYNEIKPQLLTN
GGTSDGRFIARMGAQVVELGPVNATIHKINECVNAADLQLLARMYQRVMEQLVA

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788816, Length=375, Percent_Identity=91.4666666666667, Blast_Score=724, Evalue=0.0,
Organism=Escherichia coli, GI1790395, Length=259, Percent_Identity=30.8880308880309, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1789236, Length=385, Percent_Identity=23.1168831168831, Blast_Score=72, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 41385; Mature: 41254

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: PS00758 ARGE_DAPE_CPG2_1 ; PS00759 ARGE_DAPE_CPG2_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSCPVIELAQQLIRRPSLSPDDAGCQALMIERLRAIGFTIEPMDFGDTQNFWAWRGHGET
CCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCEECCCCCCCCCCCEEECCCCCE
LAFAGHTDVVPAGDADRWINPPFEPTIRDGMLFGRGAADMKGSLAAMVVAAERFVAQYPN
EEECCCCCCCCCCCCCCCCCCCCCCCHHCCEEECCCCCCCCCHHHHHHHHHHHHHHHCCC
HRGRLAFLITSDEEASAKNGTVKVVETLMARNERLDYCLVGEPSSTEVVGDVVKNGRRGS
CCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHCCCCCE
LTCNLTIHGVQGHVAYPHLADNPVHRAAPMLAELVNIEWDKGNEFFPPTSMQIANVQSGT
EEEEEEEEECCCCEECCCCCCCCHHHHHHHHHHHHCCEECCCCCCCCCCCCEEEEECCCC
GSNNVIPGDMFVQFNFRFSTELTDEMIKSRVIALLEKYQLRYSVEWWLSGQPFLTGRGKL
CCCCCCCCCEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCEECCCCHH
VDAVVNAIEHYNEIKPQLLTNGGTSDGRFIARMGAQVVELGPVNATIHKINECVNAADLQ
HHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHCCCEEEECCCHHHHHHHHHHHCHHHHH
LLARMYQRVMEQLVA
HHHHHHHHHHHHHCC
>Mature Secondary Structure 
SCPVIELAQQLIRRPSLSPDDAGCQALMIERLRAIGFTIEPMDFGDTQNFWAWRGHGET
CCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCEECCCCCCCCCCCEEECCCCCE
LAFAGHTDVVPAGDADRWINPPFEPTIRDGMLFGRGAADMKGSLAAMVVAAERFVAQYPN
EEECCCCCCCCCCCCCCCCCCCCCCCHHCCEEECCCCCCCCCHHHHHHHHHHHHHHHCCC
HRGRLAFLITSDEEASAKNGTVKVVETLMARNERLDYCLVGEPSSTEVVGDVVKNGRRGS
CCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHCCCCCE
LTCNLTIHGVQGHVAYPHLADNPVHRAAPMLAELVNIEWDKGNEFFPPTSMQIANVQSGT
EEEEEEEEECCCCEECCCCCCCCHHHHHHHHHHHHCCEECCCCCCCCCCCCEEEEECCCC
GSNNVIPGDMFVQFNFRFSTELTDEMIKSRVIALLEKYQLRYSVEWWLSGQPFLTGRGKL
CCCCCCCCCEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCEECCCCHH
VDAVVNAIEHYNEIKPQLLTNGGTSDGRFIARMGAQVVELGPVNATIHKINECVNAADLQ
HHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHCCCEEEECCCHHHHHHHHHHHCHHHHH
LLARMYQRVMEQLVA
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA