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The map label for this gene is gltX [H]

Identifier: 238895879

GI number: 238895879

Start: 3825778

End: 3827196

Strand: Reverse

Name: gltX [H]

Synonym: KP1_4001

Alternate gene names: 238895879

Gene position: 3827196-3825778 (Counterclockwise)

Preceding gene: 238895880

Following gene: 238895876

Centisome position: 72.92

GC content: 55.46

Gene sequence:

>1419_bases
ATGAAAATCAAAACTCGCTTCGCGCCAAGCCCAACCGGCTATCTGCACGTTGGCGGTGCGCGTACTGCTCTCTATTCCTG
GCTTTTTGCCCGTAACCACGGCGGTGAGTTTGTGCTGCGTATTGAAGACACCGATCTCGAACGCTCCACCCCGGAAGCAA
TTGAAGCCATCATGGATGGCATGAACTGGCTGAATCTGCAATGGGACGAAGGCCCCTATTTCCAGACCAAACGTTTCGAT
CGTTATAACAACGTAATCGATGAGATGCTGGAAGCCGGTACCGCTTACAAATGCTATTGCTCGAAAGAACGTCTGGAAGC
GCTGCGCGAAGAGCAGATGGCGAAGGGTGAGAAGCCGCGTTACGATGGCCGCTGCCGCCATAGCCACGAGCACCATGCTG
ACGATGAGCCGTGCGTCGTGCGTTTCGCCAACCCGCAGGAAGGTTCGGTTATTTTTGACGATCAGATCCGCGGACCTATC
GAGTTCAGCAACCAGGAACTGGACGATCTGATCATTCGCCGTACCGATGGTTCCCCGACCTATAACTTCTGCGTGGTGGT
AGATGACTGGGATATGGCGATCACTCACGTGATCCGCGGCGAAGATCATATCAACAACACGCCGCGCCAGATCAATATCC
TCAAAGCGCTGAATGCGCCGGTGCCGGTTTATGCGCACGTGTCGATGATCAACGGCGATGACGGTAAAAAACTTTCCAAG
CGCCACGGCGCAGTGAGCGTGATGCAGTACCGCGACGACGGCTACCTGCCGGAAGCGCTACTCAACTATCTGGTGCGCCT
GGGCTGGTCTCATGGCGATCAGGAGATTTTCACTCGCGAAGAGATGATTGAGTTCTTCTCGCTGGGCGCGGTGAGCAAAT
CCGCCAGTGCCTTTAACACCGATAAACTGCTGTGGTTAAACCATCACTATATCAATACCCTGCCGGCGGAATATGTGGCA
ACGCATCTGCAGTGGCATATCGAGCAGGAAAATATCGACACTCGCAACGGCCCGCAGCTGGCTGAGCTGGTGAAACTGCT
GGGCGAGCGTTGCAAGACGCTGAAAGAGATGGCCCAGAGCTGCCGCTACTTCTATGAAGAGTTCGCTGAATTCGATGCCG
ACGCCGCGAAAAAACATCTGCGTCCAGTGGCGCGTCAGCCGCTGGAAGTGGTTCGCGATAAGCTGGCGGCGATTAGCGAC
TGGACGGCGGAAAACGTCCATCACGCGATTCAGGCAACTGCTGATGAGCTGGAGGTGGGGATGGGTAAAGTCGGTATGCC
GCTGCGCGTGGCGGTTACCGGGGCAGGGCAATCTCCTGCGCTAGACGTTACTGTCCATGCTATCGGTAAATCCCGTAGCG
TAGATCGTATCAATAAAGCGCTGGCTTTTATCGCTGAACGCGAAGGCCAGGCGTCCTGA

Upstream 100 bases:

>100_bases
ACAACATTGAGGAATCAGCCCACGGTGTTAGAATATTGACCCATAATTTCCACCGGACATTGCCGGCTATCGGCATCTTT
ATTAAACGTAAGGCCAATTC

Downstream 100 bases:

>100_bases
CGGCGTCGCAGCTGAAACAACGGCAGGTTACCCTGCCGTTTTTTTTGTCTGTTATTCCGGACTGGCGATGCCGAGGCGGG
CAAGAAAACCACTAATCCCT

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS [H]

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDTDLERSTPEAIEAIMDGMNWLNLQWDEGPYFQTKRFD
RYNNVIDEMLEAGTAYKCYCSKERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVIFDDQIRGPI
EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMAITHVIRGEDHINNTPRQINILKALNAPVPVYAHVSMINGDDGKKLSK
RHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEIFTREEMIEFFSLGAVSKSASAFNTDKLLWLNHHYINTLPAEYVA
THLQWHIEQENIDTRNGPQLAELVKLLGERCKTLKEMAQSCRYFYEEFAEFDADAAKKHLRPVARQPLEVVRDKLAAISD
WTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGKSRSVDRINKALAFIAEREGQAS

Sequences:

>Translated_472_residues
MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDTDLERSTPEAIEAIMDGMNWLNLQWDEGPYFQTKRFD
RYNNVIDEMLEAGTAYKCYCSKERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVIFDDQIRGPI
EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMAITHVIRGEDHINNTPRQINILKALNAPVPVYAHVSMINGDDGKKLSK
RHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEIFTREEMIEFFSLGAVSKSASAFNTDKLLWLNHHYINTLPAEYVA
THLQWHIEQENIDTRNGPQLAELVKLLGERCKTLKEMAQSCRYFYEEFAEFDADAAKKHLRPVARQPLEVVRDKLAAISD
WTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGKSRSVDRINKALAFIAEREGQAS
>Mature_472_residues
MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDTDLERSTPEAIEAIMDGMNWLNLQWDEGPYFQTKRFD
RYNNVIDEMLEAGTAYKCYCSKERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVIFDDQIRGPI
EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMAITHVIRGEDHINNTPRQINILKALNAPVPVYAHVSMINGDDGKKLSK
RHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEIFTREEMIEFFSLGAVSKSASAFNTDKLLWLNHHYINTLPAEYVA
THLQWHIEQENIDTRNGPQLAELVKLLGERCKTLKEMAQSCRYFYEEFAEFDADAAKKHLRPVARQPLEVVRDKLAAISD
WTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGKSRSVDRINKALAFIAEREGQAS

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=492, Percent_Identity=31.3008130081301, Blast_Score=228, Evalue=1e-59,
Organism=Escherichia coli, GI1788743, Length=470, Percent_Identity=95.1063829787234, Blast_Score=949, Evalue=0.0,
Organism=Escherichia coli, GI226510925, Length=244, Percent_Identity=36.4754098360656, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1786895, Length=122, Percent_Identity=27.8688524590164, Blast_Score=68, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71993394, Length=320, Percent_Identity=38.4375, Blast_Score=216, Evalue=2e-56,
Organism=Saccharomyces cerevisiae, GI6324540, Length=498, Percent_Identity=32.1285140562249, Blast_Score=245, Evalue=1e-65,
Organism=Saccharomyces cerevisiae, GI6321192, Length=229, Percent_Identity=26.6375545851528, Blast_Score=80, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6324742, Length=130, Percent_Identity=31.5384615384615, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24665321, Length=490, Percent_Identity=31.0204081632653, Blast_Score=242, Evalue=4e-64,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 53573; Mature: 53573

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDTDLERSTPEAIEAIMDG
CCCCCCCCCCCCCEEEECCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHCC
MNWLNLQWDEGPYFQTKRFDRYNNVIDEMLEAGTAYKCYCSKERLEALREEQMAKGEKPR
CCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHCCCCCC
YDGRCRHSHEHHADDEPCVVRFANPQEGSVIFDDQIRGPIEFSNQELDDLIIRRTDGSPT
CCCCCCCCCCCCCCCCCEEEEECCCCCCCEEECCCCCCCCCCCCCCCCHHEEEECCCCCC
YNFCVVVDDWDMAITHVIRGEDHINNTPRQINILKALNAPVPVYAHVSMINGDDGKKLSK
EEEEEEEECCHHHHHHHHCCCHHCCCCCCCEEHHHHCCCCCCEEEEEEEEECCCCHHHHH
RHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEIFTREEMIEFFSLGAVSKSASAFNT
HCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
DKLLWLNHHYINTLPAEYVATHLQWHIEQENIDTRNGPQLAELVKLLGERCKTLKEMAQS
CEEEEEECHHHHCCCHHHHHHHHHEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
CRYFYEEFAEFDADAAKKHLRPVARQPLEVVRDKLAAISDWTAENVHHAIQATADELEVG
HHHHHHHHHHCCHHHHHHHHHHHHHCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHC
MGKVGMPLRVAVTGAGQSPALDVTVHAIGKSRSVDRINKALAFIAEREGQAS
CCCCCCCEEEEEECCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDTDLERSTPEAIEAIMDG
CCCCCCCCCCCCCEEEECCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHCC
MNWLNLQWDEGPYFQTKRFDRYNNVIDEMLEAGTAYKCYCSKERLEALREEQMAKGEKPR
CCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHCCCCCC
YDGRCRHSHEHHADDEPCVVRFANPQEGSVIFDDQIRGPIEFSNQELDDLIIRRTDGSPT
CCCCCCCCCCCCCCCCCEEEEECCCCCCCEEECCCCCCCCCCCCCCCCHHEEEECCCCCC
YNFCVVVDDWDMAITHVIRGEDHINNTPRQINILKALNAPVPVYAHVSMINGDDGKKLSK
EEEEEEEECCHHHHHHHHCCCHHCCCCCCCEEHHHHCCCCCCEEEEEEEEECCCCHHHHH
RHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEIFTREEMIEFFSLGAVSKSASAFNT
HCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
DKLLWLNHHYINTLPAEYVATHLQWHIEQENIDTRNGPQLAELVKLLGERCKTLKEMAQS
CEEEEEECHHHHCCCHHHHHHHHHEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
CRYFYEEFAEFDADAAKKHLRPVARQPLEVVRDKLAAISDWTAENVHHAIQATADELEVG
HHHHHHHHHHCCHHHHHHHHHHHHHCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHC
MGKVGMPLRVAVTGAGQSPALDVTVHAIGKSRSVDRINKALAFIAEREGQAS
CCCCCCCEEEEEECCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA