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The map label for this gene is yfaW [H]

Identifier: 238895779

GI number: 238895779

Start: 3723983

End: 3725269

Strand: Reverse

Name: yfaW [H]

Synonym: KP1_3887

Alternate gene names: 238895779

Gene position: 3725269-3723983 (Counterclockwise)

Preceding gene: 238895780

Following gene: 238895778

Centisome position: 70.98

GC content: 59.36

Gene sequence:

>1287_bases
TTGCCTGCGCGAAAGATATTTCACGTCTGCTGGGGTGGAAAAGCCCCTTCGAACAACTCGCCTCATCATAAGGAGAACAT
CATGACCCTACCGAAAATTAAACACGTCCGCGCCTGGTTCATCGGCGGCGCCACAGCAGAGCAGGGCGCCGGCGGCGGCG
ATTACCATGACCAGGGGGCTAACCACTGGATTGACGATCATATCGCCACCCCGATCAGCAAATATAAACAGTATGAGCAG
TCCCGGCAGTCGTTTGGCATCAACGTGCTGGGGACGCTGATTGTGGAAGTGGAAGCCGGCAACGGACAGACCGGCTTTGC
GGTGTCGACGGCCGGGGAGATGGGCTGCTTTATCGTCGAAAAACATCTCAACCGCTTTATCGAAGGCAAATGCGTCAGCG
ATATCAAACTGATCCATGACCAGATGCTTAACGCCACCCTTTACTACGCGGGATCCGGCGGCCTGGTGATGAATACCATC
TCCTGTGTCGACCTGGCGCTGTGGGACCTGTTCGGGAAAGTGGTTGGCCTGCCGGTCTACAAACTGTTGGGTGGGGCGGT
GCGCGACGAAATCCAGTTCTATGCCACCGGCGCACGTCCGGACCTGGCGCAGGAGATGGGCTTTATCGGCGGCAAAATGC
CTACCCACTGGGGGCCGCACGATGGCGACGCCGGGATCCGCAAAGACGTCGCCATGGTGGCGGATATGCGCGAAAAATGC
GGCCCGGATTTCTGGTTAATGCTGGACTGCTGGATGAGCCAGGACGTTAACTATGCCACCAAACTTGCCCATGTCTGCGC
CCCTTATAACCTCAAGTGGATCGAAGAGTGTCTGCCGCCGCAGCAGTATGAAGGCTATCGCGAGCTGAAGCGCCAGGCGC
CGGCCGGGATGATGGTGACCAGCGGCGAGCACCACGGCACGCTGCAGTCCTTCCGCACCCTGTCGGAAACCGGCATCGAT
ATTATGCAGCCCGACGTCGGCTGGTGCGGCGGCTTAACCACCCTGGTGGAGATCGCGGCCATCGCCAAAGCGCGCGGGCA
GCTGGTAGTGCCCCACGGCTCGTCGGTTTACTCACATCATGCGGTGATCACCTTTACCAACACGCCGTTCAGCGAGTTTC
TGATGACCAGTCCGGACTGCGCCACGCTGCGCCCGCAGTTCGACCCGATCCTGCTCGGCGAGCCGGTGCCGGAGAGAGGG
CGCATTCATAAATCGGTCCTGGATAAACCCGGCTTTGGCGTCGAGCTCAATCGCGACTGCAACCTGAAGCGTCCTTATCA
ACACTGA

Upstream 100 bases:

>100_bases
GTATTTAACGCCAATAACGACCTGACCGCCGCCATCTCGGTGGTGGGTACCCGTCTGCAAATCAATGAAGACAATCGCGA
TTACCTGGCTGGTAAGGCGA

Downstream 100 bases:

>100_bases
TTGCGTCAGGCCGCCCGGCGGCCCGCGCCTGAGGATATTATGATGAGCAACACTCTGCTGGAGAGCGTGGTGAAGAAAAA
CCGCGCCCGCTTAATCCCCT

Product: putative dehydratase

Products: NA

Alternate protein names: RhamD [H]

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MPARKIFHVCWGGKAPSNNSPHHKENIMTLPKIKHVRAWFIGGATAEQGAGGGDYHDQGANHWIDDHIATPISKYKQYEQ
SRQSFGINVLGTLIVEVEAGNGQTGFAVSTAGEMGCFIVEKHLNRFIEGKCVSDIKLIHDQMLNATLYYAGSGGLVMNTI
SCVDLALWDLFGKVVGLPVYKLLGGAVRDEIQFYATGARPDLAQEMGFIGGKMPTHWGPHDGDAGIRKDVAMVADMREKC
GPDFWLMLDCWMSQDVNYATKLAHVCAPYNLKWIEECLPPQQYEGYRELKRQAPAGMMVTSGEHHGTLQSFRTLSETGID
IMQPDVGWCGGLTTLVEIAAIAKARGQLVVPHGSSVYSHHAVITFTNTPFSEFLMTSPDCATLRPQFDPILLGEPVPERG
RIHKSVLDKPGFGVELNRDCNLKRPYQH

Sequences:

>Translated_428_residues
MPARKIFHVCWGGKAPSNNSPHHKENIMTLPKIKHVRAWFIGGATAEQGAGGGDYHDQGANHWIDDHIATPISKYKQYEQ
SRQSFGINVLGTLIVEVEAGNGQTGFAVSTAGEMGCFIVEKHLNRFIEGKCVSDIKLIHDQMLNATLYYAGSGGLVMNTI
SCVDLALWDLFGKVVGLPVYKLLGGAVRDEIQFYATGARPDLAQEMGFIGGKMPTHWGPHDGDAGIRKDVAMVADMREKC
GPDFWLMLDCWMSQDVNYATKLAHVCAPYNLKWIEECLPPQQYEGYRELKRQAPAGMMVTSGEHHGTLQSFRTLSETGID
IMQPDVGWCGGLTTLVEIAAIAKARGQLVVPHGSSVYSHHAVITFTNTPFSEFLMTSPDCATLRPQFDPILLGEPVPERG
RIHKSVLDKPGFGVELNRDCNLKRPYQH
>Mature_427_residues
PARKIFHVCWGGKAPSNNSPHHKENIMTLPKIKHVRAWFIGGATAEQGAGGGDYHDQGANHWIDDHIATPISKYKQYEQS
RQSFGINVLGTLIVEVEAGNGQTGFAVSTAGEMGCFIVEKHLNRFIEGKCVSDIKLIHDQMLNATLYYAGSGGLVMNTIS
CVDLALWDLFGKVVGLPVYKLLGGAVRDEIQFYATGARPDLAQEMGFIGGKMPTHWGPHDGDAGIRKDVAMVADMREKCG
PDFWLMLDCWMSQDVNYATKLAHVCAPYNLKWIEECLPPQQYEGYRELKRQAPAGMMVTSGEHHGTLQSFRTLSETGIDI
MQPDVGWCGGLTTLVEIAAIAKARGQLVVPHGSSVYSHHAVITFTNTPFSEFLMTSPDCATLRPQFDPILLGEPVPERGR
IHKSVLDKPGFGVELNRDCNLKRPYQH

Specific function: Catalyzes the dehydration of L-rhamnonate to 2-keto-3- deoxy-L-rhamnonate (KDR) [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. RhamD subfamily [H]

Homologues:

Organism=Escherichia coli, GI226510960, Length=401, Percent_Identity=94.0149625935162, Blast_Score=798, Evalue=0.0,
Organism=Escherichia coli, GI48994953, Length=243, Percent_Identity=31.6872427983539, Blast_Score=93, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =4.2.1.90 [H]

Molecular weight: Translated: 47193; Mature: 47061

Theoretical pI: Translated: 6.82; Mature: 6.82

Prosite motif: PS00908 MR_MLE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPARKIFHVCWGGKAPSNNSPHHKENIMTLPKIKHVRAWFIGGATAEQGAGGGDYHDQGA
CCCHHEEEEEECCCCCCCCCCCHHHCCCCCCCHHHEEEEEECCCCCCCCCCCCCCCCCCC
NHWIDDHIATPISKYKQYEQSRQSFGINVLGTLIVEVEAGNGQTGFAVSTAGEMGCFIVE
CCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEECCCCCCCEEEECCCCCHHHHHH
KHLNRFIEGKCVSDIKLIHDQMLNATLYYAGSGGLVMNTISCVDLALWDLFGKVVGLPVY
HHHHHHHCCCHHHHHHHHHHHHHCEEEEEECCCCEEEHHHHHHHHHHHHHHHHHHCCHHH
KLLGGAVRDEIQFYATGARPDLAQEMGFIGGKMPTHWGPHDGDAGIRKDVAMVADMREKC
HHHCHHHHHCEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
GPDFWLMLDCWMSQDVNYATKLAHVCAPYNLKWIEECLPPQQYEGYRELKRQAPAGMMVT
CCCEEEEEEHHHCCCCCHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHCCCCEEEE
SGEHHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAKARGQLVVPHGSSVYSHH
CCCCCCHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCEECCE
AVITFTNTPFSEFLMTSPDCATLRPQFDPILLGEPVPERGRIHKSVLDKPGFGVELNRDC
EEEEECCCCHHHHHCCCCCCCCCCCCCCCEECCCCCCCCHHHHHHHHCCCCCCEEECCCC
NLKRPYQH
CCCCCCCC
>Mature Secondary Structure 
PARKIFHVCWGGKAPSNNSPHHKENIMTLPKIKHVRAWFIGGATAEQGAGGGDYHDQGA
CCHHEEEEEECCCCCCCCCCCHHHCCCCCCCHHHEEEEEECCCCCCCCCCCCCCCCCCC
NHWIDDHIATPISKYKQYEQSRQSFGINVLGTLIVEVEAGNGQTGFAVSTAGEMGCFIVE
CCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEECCCCCCCEEEECCCCCHHHHHH
KHLNRFIEGKCVSDIKLIHDQMLNATLYYAGSGGLVMNTISCVDLALWDLFGKVVGLPVY
HHHHHHHCCCHHHHHHHHHHHHHCEEEEEECCCCEEEHHHHHHHHHHHHHHHHHHCCHHH
KLLGGAVRDEIQFYATGARPDLAQEMGFIGGKMPTHWGPHDGDAGIRKDVAMVADMREKC
HHHCHHHHHCEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
GPDFWLMLDCWMSQDVNYATKLAHVCAPYNLKWIEECLPPQQYEGYRELKRQAPAGMMVT
CCCEEEEEEHHHCCCCCHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHCCCCEEEE
SGEHHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAKARGQLVVPHGSSVYSHH
CCCCCCHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCEECCE
AVITFTNTPFSEFLMTSPDCATLRPQFDPILLGEPVPERGRIHKSVLDKPGFGVELNRDC
EEEEECCCCHHHHHCCCCCCCCCCCCCCCEECCCCCCCCHHHHHHHHCCCCCCEEECCCC
NLKRPYQH
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA