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The map label for this gene is alkB [H]

Identifier: 238895759

GI number: 238895759

Start: 3695821

End: 3696474

Strand: Reverse

Name: alkB [H]

Synonym: KP1_3866

Alternate gene names: 238895759

Gene position: 3696474-3695821 (Counterclockwise)

Preceding gene: 238895760

Following gene: 238895758

Centisome position: 70.43

GC content: 63.3

Gene sequence:

>654_bases
ATGCTCGATCTGTTTAGCGATACCCCACCGTGGCAGGAGCCGCTGGCCCCCGGCGCGGTGGTGCTGCGGCGATTTGCCCG
CGAACGCGCCCCGGCGCTGCTGCAGGCGATAGCCGACGTCGCCAGTCAGTCGCCGTTTCGCCAGATGGTCACGCCGGGCG
GGTACACCATGTCGGTCGCCATGACCAACTGCGGCGCGCTGGGCTGGACTACCGATCGCCACGGCTATTTGTACGCCCCG
GTCGACCCGGTAACCGATCAAACCTGGCCGCCGATGCCTGCCGTCTTTCATGAACTGGCGCTGGCGGCGGCCGCCGCCGG
CGGCTATCCGGAGTTTTCCCCTGACGCCTGTTTGATTAACCGCTATTGTCCTGGCGCAAAACTGTCGTTGCATCAGGATA
AAGATGAACAGGATCTGCGGGCGCCGATTGTCTCGGTCTCGCTGGGCTTGCCGGCCATCTTTCAGTTTGGCGGCTTGCAA
CGCAGCGACCCGCTGCAGCGGCTGCTGCTGGAGCATGGCGATGTGGTGGTCTGGGGCGGCGAGTCGCGCCTGTTTTATCA
TGGCATTCAGCCGCTGAAAGCGGGGCATCATCCGGAAACGGACGATTGTCGTTACAATCTCACCTTCCGACAGGCGGGTG
GTCGTCAGTATTAA

Upstream 100 bases:

>100_bases
ACCGGGTGGTGCGTGAAGATGGCGCCCTGTCCGGTTATCGCTGGGGAACGGCGCGAAAAGCGCAGCTGCTGGCGCGCGAG
GCCCAACATGAGGAGGAGTA

Downstream 100 bases:

>100_bases
AATAAGAATTATTCTTGATGTCATCGGCGAGCCGTTTAAACTGCTGGCTGTTTTGCTTTTGCGGATAGCCTGTTATGGAA
CTTCTCTCTCTCGTCTGGCG

Product: oxidative demethylase of N1-methyladenine or N3-methylcytosine DNA lesions

Products: NA

Alternate protein names: Alkylated DNA repair protein AlkB [H]

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MLDLFSDTPPWQEPLAPGAVVLRRFARERAPALLQAIADVASQSPFRQMVTPGGYTMSVAMTNCGALGWTTDRHGYLYAP
VDPVTDQTWPPMPAVFHELALAAAAAGGYPEFSPDACLINRYCPGAKLSLHQDKDEQDLRAPIVSVSLGLPAIFQFGGLQ
RSDPLQRLLLEHGDVVVWGGESRLFYHGIQPLKAGHHPETDDCRYNLTFRQAGGRQY

Sequences:

>Translated_217_residues
MLDLFSDTPPWQEPLAPGAVVLRRFARERAPALLQAIADVASQSPFRQMVTPGGYTMSVAMTNCGALGWTTDRHGYLYAP
VDPVTDQTWPPMPAVFHELALAAAAAGGYPEFSPDACLINRYCPGAKLSLHQDKDEQDLRAPIVSVSLGLPAIFQFGGLQ
RSDPLQRLLLEHGDVVVWGGESRLFYHGIQPLKAGHHPETDDCRYNLTFRQAGGRQY
>Mature_217_residues
MLDLFSDTPPWQEPLAPGAVVLRRFARERAPALLQAIADVASQSPFRQMVTPGGYTMSVAMTNCGALGWTTDRHGYLYAP
VDPVTDQTWPPMPAVFHELALAAAAAGGYPEFSPDACLINRYCPGAKLSLHQDKDEQDLRAPIVSVSLGLPAIFQFGGLQ
RSDPLQRLLLEHGDVVVWGGESRLFYHGIQPLKAGHHPETDDCRYNLTFRQAGGRQY

Specific function: Dioxygenase that repairs alkylated DNA and RNA containing 3-methylcytosine or 1-methyladenine by oxidative demethylation. Has highest activity towards 3-methylcytosine. Has lower activity towards alkylated DNA containing ethenoadenine, and no detectable a

COG id: COG3145

COG function: function code L; Alkylated DNA repair protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe2OG dioxygenase domain [H]

Homologues:

Organism=Homo sapiens, GI87298840, Length=123, Percent_Identity=32.520325203252, Blast_Score=71, Evalue=6e-13,
Organism=Escherichia coli, GI1788541, Length=216, Percent_Identity=79.1666666666667, Blast_Score=356, Evalue=1e-100,
Organism=Drosophila melanogaster, GI45555401, Length=126, Percent_Identity=36.5079365079365, Blast_Score=76, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004574 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 23829; Mature: 23829

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDLFSDTPPWQEPLAPGAVVLRRFARERAPALLQAIADVASQSPFRQMVTPGGYTMSVA
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCEEEEEE
MTNCGALGWTTDRHGYLYAPVDPVTDQTWPPMPAVFHELALAAAAAGGYPEFSPDACLIN
ECCCCCCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEHC
RYCPGAKLSLHQDKDEQDLRAPIVSVSLGLPAIFQFGGLQRSDPLQRLLLEHGDVVVWGG
CCCCCCCEECCCCCCHHHHHCCHHHHHCCCHHHHHHCCCCCCCHHHHHHHHCCCEEEECC
ESRLFYHGIQPLKAGHHPETDDCRYNLTFRQAGGRQY
CCEEEEECCCHHCCCCCCCCCCCEEEEEEECCCCCCC
>Mature Secondary Structure
MLDLFSDTPPWQEPLAPGAVVLRRFARERAPALLQAIADVASQSPFRQMVTPGGYTMSVA
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCEEEEEE
MTNCGALGWTTDRHGYLYAPVDPVTDQTWPPMPAVFHELALAAAAAGGYPEFSPDACLIN
ECCCCCCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEHC
RYCPGAKLSLHQDKDEQDLRAPIVSVSLGLPAIFQFGGLQRSDPLQRLLLEHGDVVVWGG
CCCCCCCEECCCCCCHHHHHCCHHHHHCCCHHHHHHCCCCCCCHHHHHHHHCCCEEEECC
ESRLFYHGIQPLKAGHHPETDDCRYNLTFRQAGGRQY
CCEEEEECCCHHCCCCCCCCCCCEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3536913; 9097040; 9278503; 3887409; 7928996 [H]