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The map label for this gene is yohI [H]

Identifier: 238895707

GI number: 238895707

Start: 3635873

End: 3636805

Strand: Reverse

Name: yohI [H]

Synonym: KP1_3808

Alternate gene names: 238895707

Gene position: 3636805-3635873 (Counterclockwise)

Preceding gene: 238895712

Following gene: 238895706

Centisome position: 69.29

GC content: 59.27

Gene sequence:

>933_bases
ATGCGTGTTCTACTGGCGCCGATGGAAGGCGTGCTCGATTCGCTGGTGCGCGAGCTGCTGACCGAGGTTAACGATTACGA
TCTCTGCATCACCGAATTTCTGCGCGTGGTGGATCAACTGCTGCCGGTAAAATCGTTCTACAGGCTATGCCCGGAACTGC
ATCACCAGAGCCGCACGCCGTCCGGCACCCGCGTACGCGTCCAGCTGCTGGGACAATATCCCGAGTGGCTGGCGGAAAAT
GCCGCCCGCGCGGTGGCGCTGGGTTCATGGGGGGTGGATTTAAACTGCGGCTGTCCGTCGAAGCTGGTAAACGGCAGCGG
CGGCGGGGCAACGCTGCTGAAAGATCCAGAGTTGATCTATCGCGGCGCGAAAGCCATGCGAGAGGCGGTTCCCGAGCATC
TGCCGGTGACGGTGAAAGTTCGTCTTGGCTGGGACAGCGGTGAACGGCGCTTTGAAATCGCCGACGCGGTGCAGCAGGCG
GGGGCCAGCGAACTGGTGGTTCACGGCCGTACCAAAGAAGATGGCTACAAAGCCGAACGCATCAACTGGCAGGCGATCGG
CGAGATCCGCCAGCGCCTGACTATCCCGGTGGTCGCCAACGGTGAAATCTGGGACTGGCAAAGCGCCCAGGATTGTATGG
CTGTCACAGGCTGCGATTCGGTGATGATTGGCCGCGGGGCGCTCAATGTGCCGAACCTCAGCCGGGTGATCAAATACAAT
GAACCGCGCATGCCGTGGCCGCAGGTGGTCCAGCTGTTGCAAAAATATACCCGCCTGGAAAAGCAGGGTGATACCGGCCT
GTATCATGTGGCGAGAATTAAGCAGTGGTTAGGCTATTTACGCAAGGAATATACCGAGGCGCTGACCCTGTTTAATGAAA
TTCGCGCCCTGCAAACGTCTGCTGAAATAGCGGTGGCGATCGGCCGCTATTAA

Upstream 100 bases:

>100_bases
GCGTGCGGGGAACGGCGTGCGTCTGGTCCGACGCATTGAGCGCTTTCAACCAGCGCCCGGAGGCGCTACCATAGCGCCTC
TTTTTTTTCCGGGTAACGAT

Downstream 100 bases:

>100_bases
CCGCCATACTGTTATTCGCTTCACATATTATTACTTGCATGCCTATTGATGATAGGCATGGGTTGAATAAAGTGATAATG
TGCGGTCAATCAATTCAGGA

Product: tRNA-dihydrouridine synthase C

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFLRVVDQLLPVKSFYRLCPELHHQSRTPSGTRVRVQLLGQYPEWLAEN
AARAVALGSWGVDLNCGCPSKLVNGSGGGATLLKDPELIYRGAKAMREAVPEHLPVTVKVRLGWDSGERRFEIADAVQQA
GASELVVHGRTKEDGYKAERINWQAIGEIRQRLTIPVVANGEIWDWQSAQDCMAVTGCDSVMIGRGALNVPNLSRVIKYN
EPRMPWPQVVQLLQKYTRLEKQGDTGLYHVARIKQWLGYLRKEYTEALTLFNEIRALQTSAEIAVAIGRY

Sequences:

>Translated_310_residues
MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFLRVVDQLLPVKSFYRLCPELHHQSRTPSGTRVRVQLLGQYPEWLAEN
AARAVALGSWGVDLNCGCPSKLVNGSGGGATLLKDPELIYRGAKAMREAVPEHLPVTVKVRLGWDSGERRFEIADAVQQA
GASELVVHGRTKEDGYKAERINWQAIGEIRQRLTIPVVANGEIWDWQSAQDCMAVTGCDSVMIGRGALNVPNLSRVIKYN
EPRMPWPQVVQLLQKYTRLEKQGDTGLYHVARIKQWLGYLRKEYTEALTLFNEIRALQTSAEIAVAIGRY
>Mature_310_residues
MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFLRVVDQLLPVKSFYRLCPELHHQSRTPSGTRVRVQLLGQYPEWLAEN
AARAVALGSWGVDLNCGCPSKLVNGSGGGATLLKDPELIYRGAKAMREAVPEHLPVTVKVRLGWDSGERRFEIADAVQQA
GASELVVHGRTKEDGYKAERINWQAIGEIRQRLTIPVVANGEIWDWQSAQDCMAVTGCDSVMIGRGALNVPNLSRVIKYN
EPRMPWPQVVQLLQKYTRLEKQGDTGLYHVARIKQWLGYLRKEYTEALTLFNEIRALQTSAEIAVAIGRY

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusC subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=208, Percent_Identity=31.25, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI8923374, Length=287, Percent_Identity=29.2682926829268, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI40807366, Length=233, Percent_Identity=30.4721030042918, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI239788483, Length=180, Percent_Identity=32.7777777777778, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI239788462, Length=180, Percent_Identity=32.7777777777778, Blast_Score=72, Evalue=7e-13,
Organism=Escherichia coli, GI1788462, Length=307, Percent_Identity=85.0162866449512, Blast_Score=552, Evalue=1e-159,
Organism=Escherichia coli, GI1789660, Length=281, Percent_Identity=31.6725978647687, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI145693211, Length=316, Percent_Identity=25.9493670886076, Blast_Score=65, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI25144369, Length=168, Percent_Identity=35.7142857142857, Blast_Score=98, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17507177, Length=154, Percent_Identity=32.4675324675325, Blast_Score=68, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17543114, Length=220, Percent_Identity=26.3636363636364, Blast_Score=65, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6323560, Length=199, Percent_Identity=31.6582914572864, Blast_Score=88, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6323437, Length=146, Percent_Identity=27.3972602739726, Blast_Score=63, Evalue=7e-11,
Organism=Drosophila melanogaster, GI19921524, Length=145, Percent_Identity=35.1724137931034, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24585320, Length=246, Percent_Identity=32.520325203252, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24580595, Length=170, Percent_Identity=30.5882352941176, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI19920448, Length=170, Percent_Identity=30.5882352941176, Blast_Score=79, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: 1.-.-.-

Molecular weight: Translated: 34798; Mature: 34798

Theoretical pI: Translated: 7.81; Mature: 7.81

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFLRVVDQLLPVKSFYRLCPELHHQSRTP
CEEEECHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCC
SGTRVRVQLLGQYPEWLAENAARAVALGSWGVDLNCGCPSKLVNGSGGGATLLKDPELIY
CCCEEEEEEHHCCHHHHHHHHHHEEEEECCCCEECCCCCHHHCCCCCCCCEEECCHHHHH
RGAKAMREAVPEHLPVTVKVRLGWDSGERRFEIADAVQQAGASELVVHGRTKEDGYKAER
HHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCHHH
INWQAIGEIRQRLTIPVVANGEIWDWQSAQDCMAVTGCDSVMIGRGALNVPNLSRVIKYN
CCHHHHHHHHHHEECEEEECCCCCCCCCCHHHHHHCCCCCEEECCCCCCCCCHHHHHCCC
EPRMPWPQVVQLLQKYTRLEKQGDTGLYHVARIKQWLGYLRKEYTEALTLFNEIRALQTS
CCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEIAVAIGRY
HHEEEEECCC
>Mature Secondary Structure
MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFLRVVDQLLPVKSFYRLCPELHHQSRTP
CEEEECHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCC
SGTRVRVQLLGQYPEWLAENAARAVALGSWGVDLNCGCPSKLVNGSGGGATLLKDPELIY
CCCEEEEEEHHCCHHHHHHHHHHEEEEECCCCEECCCCCHHHCCCCCCCCEEECCHHHHH
RGAKAMREAVPEHLPVTVKVRLGWDSGERRFEIADAVQQAGASELVVHGRTKEDGYKAER
HHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCHHH
INWQAIGEIRQRLTIPVVANGEIWDWQSAQDCMAVTGCDSVMIGRGALNVPNLSRVIKYN
CCHHHHHHHHHHEECEEEECCCCCCCCCCHHHHHHCCCCCEEECCCCCCCCCHHHHHCCC
EPRMPWPQVVQLLQKYTRLEKQGDTGLYHVARIKQWLGYLRKEYTEALTLFNEIRALQTS
CCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEIAVAIGRY
HHEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]