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The map label for this gene is dgoA [C]

Identifier: 238895660

GI number: 238895660

Start: 3580419

End: 3581573

Strand: Reverse

Name: dgoA [C]

Synonym: KP1_3756

Alternate gene names: 238895660

Gene position: 3581573-3580419 (Counterclockwise)

Preceding gene: 238895661

Following gene: 238895659

Centisome position: 68.24

GC content: 59.48

Gene sequence:

>1155_bases
ATGAAAATTGAATCTGTGAATGTCACCGTTTTCCAGTATCCCACTCGTCGGGTTTCCGACAGCGCCGGGCACTCCCATCC
GGGGGCTGAAAGCATGGCCAAAATGGCGATGCTGACCATCACCGCTGATGATGGGGCGCAGGGTTTTTCGTTTGCGCCTC
CGGAAGTCGTGCGCCCGTTCGTGGTGAATACTTTTTTTCGCAAAGTGCTGGTGGGGCAGGACCCGTTTAACCGTGAGCGT
ATCTGGCAGGATCTTAACCACTGGCAGCGCGGCAGCGCACATCAGCTAACGGAGCGCGCGCTGTCGTTTGTTGAACAAGC
GCTGTGGGACCTGATTGGCCGCAGCCTCAGGATGCCGGTCTACAAGCTGCTTGGCGGCTATCGCGACACCGTCCCGGCTT
ACGGTAGCACCATGTGCGGCGACGATCTACCGGGCGGCCTGTCGACCCCGGAAGAGTATGCCGCCTTTGCCGAAAAGCTG
GTCGCCCGCGGCTACAAGGCCATCAAACTGCACACCTGGATGCCGCCAATCTCTTTTGCGCCCAATCCCAAAATGGATAT
TAAAGCCTGCGCGGCGGTGCGCGAAGCGGTAGGGCCGGATATTGACCTGATGATTGATGGCTATCACTGGTACAGCCGCG
CCGAGGCGCTGTGGATCGGCAAAGAGCTGGAAAAACTCAATTTCGCCTGGTTTGAAGAGCCGATGGAGGAGGATTCGATG
TCCTCCTATGCGTGGCTGGCGGAGAACCTGTCGATTCCCATCGTCGGGCCGGAGAGCTTCGGCGGCAAGCACCACATGCG
CGCCGAATGGGTGAAAGCCGGGGCCTGCGACATTCTGCGCGCCGGCGCTAACGGCGTGGGGGGGATTACCCCGACCATGA
AGGTGGCGGCGCTGGCGGAATCTTTCGGCATGGATTGCGAAGTCCACGGCAACGGAGCGGCAAGCCTGGCGGTGGTGGGC
GCGATCCGCAACTGCCGCTGGTACGAGCGCGGCCTGCTGCATCCGTTCCTCGACTACGATGAACCGGCGGCCTATCTCAA
CAGCATTGTCGACCCGATGGATGACCAGGGATTTGTGCATTTGTCACAACGTCCGGGTCTCGGTGAAGACATTAATTTTG
CGTATATCGAAGCCAATACCGTTAGCCACGACTGA

Upstream 100 bases:

>100_bases
ATTGTGCTTAGATTATATATTGAAACGTATTTTCAATAATGTGAAACCGCTTTGCCGATTTGCCTCTGTCGATCGGATTT
GGCCGTGATAAGGATTGAGC

Downstream 100 bases:

>100_bases
TTTAAAAAAATAAATACCCGATGGCGCTGCGCTTATCGAGCCTGCGGCCCGGTAGACCGGATAAGCCGAAAATGCCGCCA
TCCGGCAACATGTACCCTAC

Product: putative mandelate racemase / muconate lactonizing enzyme

Products: Glyceraldehyde 3-phosphate; Pyruvate [C]

Alternate protein names: AE epimerase; AEE [H]

Number of amino acids: Translated: 384; Mature: 384

Protein sequence:

>384_residues
MKIESVNVTVFQYPTRRVSDSAGHSHPGAESMAKMAMLTITADDGAQGFSFAPPEVVRPFVVNTFFRKVLVGQDPFNRER
IWQDLNHWQRGSAHQLTERALSFVEQALWDLIGRSLRMPVYKLLGGYRDTVPAYGSTMCGDDLPGGLSTPEEYAAFAEKL
VARGYKAIKLHTWMPPISFAPNPKMDIKACAAVREAVGPDIDLMIDGYHWYSRAEALWIGKELEKLNFAWFEEPMEEDSM
SSYAWLAENLSIPIVGPESFGGKHHMRAEWVKAGACDILRAGANGVGGITPTMKVAALAESFGMDCEVHGNGAASLAVVG
AIRNCRWYERGLLHPFLDYDEPAAYLNSIVDPMDDQGFVHLSQRPGLGEDINFAYIEANTVSHD

Sequences:

>Translated_384_residues
MKIESVNVTVFQYPTRRVSDSAGHSHPGAESMAKMAMLTITADDGAQGFSFAPPEVVRPFVVNTFFRKVLVGQDPFNRER
IWQDLNHWQRGSAHQLTERALSFVEQALWDLIGRSLRMPVYKLLGGYRDTVPAYGSTMCGDDLPGGLSTPEEYAAFAEKL
VARGYKAIKLHTWMPPISFAPNPKMDIKACAAVREAVGPDIDLMIDGYHWYSRAEALWIGKELEKLNFAWFEEPMEEDSM
SSYAWLAENLSIPIVGPESFGGKHHMRAEWVKAGACDILRAGANGVGGITPTMKVAALAESFGMDCEVHGNGAASLAVVG
AIRNCRWYERGLLHPFLDYDEPAAYLNSIVDPMDDQGFVHLSQRPGLGEDINFAYIEANTVSHD
>Mature_384_residues
MKIESVNVTVFQYPTRRVSDSAGHSHPGAESMAKMAMLTITADDGAQGFSFAPPEVVRPFVVNTFFRKVLVGQDPFNRER
IWQDLNHWQRGSAHQLTERALSFVEQALWDLIGRSLRMPVYKLLGGYRDTVPAYGSTMCGDDLPGGLSTPEEYAAFAEKL
VARGYKAIKLHTWMPPISFAPNPKMDIKACAAVREAVGPDIDLMIDGYHWYSRAEALWIGKELEKLNFAWFEEPMEEDSM
SSYAWLAENLSIPIVGPESFGGKHHMRAEWVKAGACDILRAGANGVGGITPTMKVAALAESFGMDCEVHGNGAASLAVVG
AIRNCRWYERGLLHPFLDYDEPAAYLNSIVDPMDDQGFVHLSQRPGLGEDINFAYIEANTVSHD

Specific function: Catalyzes the epimerization of L-Ala-D-Glu to L-Ala-L- Glu and has probably a role in the metabolism of the murein peptide, of which L-Ala-D-Glu is a component. Is also able to catalyze the epimerization of L-Ala-D-Asp [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI48994953, Length=319, Percent_Identity=27.8996865203762, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI226510960, Length=345, Percent_Identity=24.6376811594203, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: 4.1.2.21; 4.2.1.6 [C]

Molecular weight: Translated: 42507; Mature: 42507

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIESVNVTVFQYPTRRVSDSAGHSHPGAESMAKMAMLTITADDGAQGFSFAPPEVVRPF
CCEEEEEEEEEECCCHHHCCCCCCCCCCHHHHHHEEEEEEEECCCCCCCCCCCHHHHHHH
VVNTFFRKVLVGQDPFNRERIWQDLNHWQRGSAHQLTERALSFVEQALWDLIGRSLRMPV
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHH
YKLLGGYRDTVPAYGSTMCGDDLPGGLSTPEEYAAFAEKLVARGYKAIKLHTWMPPISFA
HHHHCCCHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCC
PNPKMDIKACAAVREAVGPDIDLMIDGYHWYSRAEALWIGKELEKLNFAWFEEPMEEDSM
CCCCCHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
SSYAWLAENLSIPIVGPESFGGKHHMRAEWVKAGACDILRAGANGVGGITPTMKVAALAE
HHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHCCCHHHHHCCCCCCCCCCHHHHHHHHHH
SFGMDCEVHGNGAASLAVVGAIRNCRWYERGLLHPFLDYDEPAAYLNSIVDPMDDQGFVH
HHCCCEEEECCCCHHHHHHHHHHHCHHHHHCCCCHHCCCCCHHHHHHHHCCCCCCCCEEE
LSQRPGLGEDINFAYIEANTVSHD
EECCCCCCCCCCEEEEEECCCCCC
>Mature Secondary Structure
MKIESVNVTVFQYPTRRVSDSAGHSHPGAESMAKMAMLTITADDGAQGFSFAPPEVVRPF
CCEEEEEEEEEECCCHHHCCCCCCCCCCHHHHHHEEEEEEEECCCCCCCCCCCHHHHHHH
VVNTFFRKVLVGQDPFNRERIWQDLNHWQRGSAHQLTERALSFVEQALWDLIGRSLRMPV
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHH
YKLLGGYRDTVPAYGSTMCGDDLPGGLSTPEEYAAFAEKLVARGYKAIKLHTWMPPISFA
HHHHCCCHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCC
PNPKMDIKACAAVREAVGPDIDLMIDGYHWYSRAEALWIGKELEKLNFAWFEEPMEEDSM
CCCCCHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
SSYAWLAENLSIPIVGPESFGGKHHMRAEWVKAGACDILRAGANGVGGITPTMKVAALAE
HHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHCCCHHHHHCCCCCCCCCCHHHHHHHHHH
SFGMDCEVHGNGAASLAVVGAIRNCRWYERGLLHPFLDYDEPAAYLNSIVDPMDDQGFVH
HHCCCEEEECCCCHHHHHHHHHHHCHHHHHCCCCHHCCCCCHHHHHHHHCCCCCCCCEEE
LSQRPGLGEDINFAYIEANTVSHD
EECCCCCCCCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-Dehydro-3-deoxy-D-galactonate 6-phosphate [C]

Specific reaction: 2-Dehydro-3-deoxy-D-galactonate 6-phosphate <==> Glyceraldehyde 3-phosphate + Pyruvate [C]

General reaction: elimination of H2O C-O bond; Cleavage [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]