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The map label for this gene is wcaH [H]

Identifier: 238895621

GI number: 238895621

Start: 3530461

End: 3530919

Strand: Reverse

Name: wcaH [H]

Synonym: KP1_3708

Alternate gene names: 238895621

Gene position: 3530919-3530461 (Counterclockwise)

Preceding gene: 238895622

Following gene: 238895620

Centisome position: 67.27

GC content: 31.81

Gene sequence:

>459_bases
ATGTTTTTAGATGATGATGCATTTAAAAATGTAATAAAGAATACGCCGCTAATCTCAATTGATTTGATCATACAAAATGA
GAAAGGTGAGTATTTAGTTGGTAAAAGAAACAACCGCCCCGCAAGGGGCTTTTGGTTTGTTCCTGGAGGTCGCATACAAA
AAAATGAGACATTAAATAATGGCTTCACCAGATTAATTCAAAATGAAATTGGCATTGAGATGCTGAGAAATGAAGCCACT
TTTCTCGGTGTTTTTGAACATTTCTATGATGATAATTACTTTAATAGTGAATTTAGTACTCACTATATAGTATTGGCTTA
TAAAATATCTATTTTATCCAATGGCTTAGTATTTCCGCATGAACAGCATAATGAATATCTCTGGATGAGTGCGGATGAAA
TCCTTAATAATGATTTAGTGCATTTTAATACTAAAGCTTATTTTATGGATGTTTCTTAG

Upstream 100 bases:

>100_bases
TTGGAAGGCTTAGGTTGGAAGTATCAAATTAAGCTTGAGGCAGGTTTAACTCGGACTTATGAGTGGTTTTTAAAGAATCA
GCAAAGCTTCCGGAGTTAAT

Downstream 100 bases:

>100_bases
GACTTTTAGGTATGAAATTACTTGTATATGGAATCAATTATCGTCCTGAATTAACTGGTATTGGCAAGTATAGTGGTGAA
ATGGTGAAGTGGTTTGTTGA

Product: GDP-mannose mannosyl hydrolase

Products: GDP; mannose [C]

Alternate protein names: GDPMH; Colanic acid biosynthesis protein wcaH [H]

Number of amino acids: Translated: 152; Mature: 152

Protein sequence:

>152_residues
MFLDDDAFKNVIKNTPLISIDLIIQNEKGEYLVGKRNNRPARGFWFVPGGRIQKNETLNNGFTRLIQNEIGIEMLRNEAT
FLGVFEHFYDDNYFNSEFSTHYIVLAYKISILSNGLVFPHEQHNEYLWMSADEILNNDLVHFNTKAYFMDVS

Sequences:

>Translated_152_residues
MFLDDDAFKNVIKNTPLISIDLIIQNEKGEYLVGKRNNRPARGFWFVPGGRIQKNETLNNGFTRLIQNEIGIEMLRNEAT
FLGVFEHFYDDNYFNSEFSTHYIVLAYKISILSNGLVFPHEQHNEYLWMSADEILNNDLVHFNTKAYFMDVS
>Mature_152_residues
MFLDDDAFKNVIKNTPLISIDLIIQNEKGEYLVGKRNNRPARGFWFVPGGRIQKNETLNNGFTRLIQNEIGIEMLRNEAT
FLGVFEHFYDDNYFNSEFSTHYIVLAYKISILSNGLVFPHEQHNEYLWMSADEILNNDLVHFNTKAYFMDVS

Specific function: It could participate in the regulation of cell wall biosynthesis by influencing the concentration of GDP-mannose or GDP-glucose in the cell. May be involved in the degradation of GDP-mannose and GDP-glucose, diverting the GDP to the synthesis of GDP-fucos

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI87082031, Length=149, Percent_Identity=47.6510067114094, Blast_Score=177, Evalue=2e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021161
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: NA

Molecular weight: Translated: 17792; Mature: 17792

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLDDDAFKNVIKNTPLISIDLIIQNEKGEYLVGKRNNRPARGFWFVPGGRIQKNETLNN
CCCCCHHHHHHHCCCCEEEEEEEEECCCCCEEEECCCCCCCCEEEECCCCEEECCCCHHH
GFTRLIQNEIGIEMLRNEATFLGVFEHFYDDNYFNSEFSTHYIVLAYKISILSNGLVFPH
HHHHHHHHHHCHHHHHCCCHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEECCCCEECCC
EQHNEYLWMSADEILNNDLVHFNTKAYFMDVS
CCCCCEEEEEHHHHHCCCEEEEECEEEEEECC
>Mature Secondary Structure
MFLDDDAFKNVIKNTPLISIDLIIQNEKGEYLVGKRNNRPARGFWFVPGGRIQKNETLNN
CCCCCHHHHHHHCCCCEEEEEEEEECCCCCEEEECCCCCCCCEEEECCCCEEECCCCHHH
GFTRLIQNEIGIEMLRNEATFLGVFEHFYDDNYFNSEFSTHYIVLAYKISILSNGLVFPH
HHHHHHHHHHCHHHHHCCCHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEECCCCEECCC
EQHNEYLWMSADEILNNDLVHFNTKAYFMDVS
CCCCCEEEEEHHHHHCCCEEEEECEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GDP-mannose; H2O [C]

Specific reaction: GDP-mannose + H2O = GDP + mannose [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]