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The map label for this gene is wcaG [H]

Identifier: 238895622

GI number: 238895622

Start: 3530921

End: 3531961

Strand: Reverse

Name: wcaG [H]

Synonym: KP1_3709

Alternate gene names: 238895622

Gene position: 3531961-3530921 (Counterclockwise)

Preceding gene: 238895623

Following gene: 238895621

Centisome position: 67.29

GC content: 37.75

Gene sequence:

>1041_bases
TTGAGGCAGCTAAAAAGCATTCTCTTCTCAAATCACATGGTTACGAGGTTTCAATTTCTCTGGAGTAAAGAAATGACTAT
TAAGAAGAGAGTTTTTGTTGCTGGGTATAATGGTATGGTTGGTTCAGCAATCGTAAGACAATTGAAAAATAGAGACGACA
TTGAACTCGTACTGCGTTCACGTCAGGAGCTCAACCTGCTTAATGCTCAAGACGTAAATCATTTTTTCGCTAATGAGCGT
ATTAATGAAGTATATTTAGCTGCTGCAAAAGTTGGCGGAATAGTCGCGAATAACACTTATCCAGCTGATTTTATTTATGA
AAATATTCTTATTGAAGCAAACATCATACATGCTGCACATGTTAATGATGTAAATAAAATAATGTTTTTAGGCTCATCAT
GTATATACCCTAAATTTGCTAAGCAACCGATAAGCGAGTCTGAATTACTGCAAGGAACACTTGAATCTACAAATGAGCCG
TATGCTATAGCAAAAATTGCCGGTATAAAACTCTGCGAGTCTTATAATCGGCAACATAATAGAGATTATCGTTCAGTAAT
GCCAACTAATTTATATGGAGAAAATGATAATTTCCATCCTTCTAACTCGCATGTAATTCCTGCATTATTGCGGCGCTTTC
ATGAGGCAAAGTTAGAAGGACTACCACAAGTCGTTGTTTGGGGTAGTGGAACGCCGATGCGTGAGTTTCTACATGTTGAT
GACATGGCTGCTGCATCTATTTATGTCATGGACCTTGATAAATCTATTTGGCAGGAGCAAACACAACCAATGTTGTCACA
TATTAACGTTGGTACTGGAGTTGATTGTTCTATTAGGGAATTAGCCGAAACCATATCAAAAGTGGTTGGTTATCGCGGAG
AGGTAGTTTTTGATTCAACCAAACCTGATGGCACTCCAAGAAAATTATTAGATGTTTCGAGATTGGAAGGCTTAGGTTGG
AAGTATCAAATTAAGCTTGAGGCAGGTTTAACTCGGACTTATGAGTGGTTTTTAAAGAATCAGCAAAGCTTCCGGAGTTA
A

Upstream 100 bases:

>100_bases
GAAACCTTGCTTGGAGACCCTTCGAAAGCCCACGATAAATTAGGATGGAAACCTGAAACAACATTACAAGAAATGGTTGT
CGAAATGGTAAGAAAGGATC

Downstream 100 bases:

>100_bases
TATGTTTTTAGATGATGATGCATTTAAAAATGTAATAAAGAATACGCCGCTAATCTCAATTGATTTGATCATACAAAATG
AGAAAGGTGAGTATTTAGTT

Product: GDP-fucose synthetase

Products: NA

Alternate protein names: GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase [H]

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MRQLKSILFSNHMVTRFQFLWSKEMTIKKRVFVAGYNGMVGSAIVRQLKNRDDIELVLRSRQELNLLNAQDVNHFFANER
INEVYLAAAKVGGIVANNTYPADFIYENILIEANIIHAAHVNDVNKIMFLGSSCIYPKFAKQPISESELLQGTLESTNEP
YAIAKIAGIKLCESYNRQHNRDYRSVMPTNLYGENDNFHPSNSHVIPALLRRFHEAKLEGLPQVVVWGSGTPMREFLHVD
DMAAASIYVMDLDKSIWQEQTQPMLSHINVGTGVDCSIRELAETISKVVGYRGEVVFDSTKPDGTPRKLLDVSRLEGLGW
KYQIKLEAGLTRTYEWFLKNQQSFRS

Sequences:

>Translated_346_residues
MRQLKSILFSNHMVTRFQFLWSKEMTIKKRVFVAGYNGMVGSAIVRQLKNRDDIELVLRSRQELNLLNAQDVNHFFANER
INEVYLAAAKVGGIVANNTYPADFIYENILIEANIIHAAHVNDVNKIMFLGSSCIYPKFAKQPISESELLQGTLESTNEP
YAIAKIAGIKLCESYNRQHNRDYRSVMPTNLYGENDNFHPSNSHVIPALLRRFHEAKLEGLPQVVVWGSGTPMREFLHVD
DMAAASIYVMDLDKSIWQEQTQPMLSHINVGTGVDCSIRELAETISKVVGYRGEVVFDSTKPDGTPRKLLDVSRLEGLGW
KYQIKLEAGLTRTYEWFLKNQQSFRS
>Mature_346_residues
MRQLKSILFSNHMVTRFQFLWSKEMTIKKRVFVAGYNGMVGSAIVRQLKNRDDIELVLRSRQELNLLNAQDVNHFFANER
INEVYLAAAKVGGIVANNTYPADFIYENILIEANIIHAAHVNDVNKIMFLGSSCIYPKFAKQPISESELLQGTLESTNEP
YAIAKIAGIKLCESYNRQHNRDYRSVMPTNLYGENDNFHPSNSHVIPALLRRFHEAKLEGLPQVVVWGSGTPMREFLHVD
DMAAASIYVMDLDKSIWQEQTQPMLSHINVGTGVDCSIRELAETISKVVGYRGEVVFDSTKPDGTPRKLLDVSRLEGLGW
KYQIKLEAGLTRTYEWFLKNQQSFRS

Specific function: Two step NADP-dependent conversion of GDP-4-dehydro-6- deoxy-D-mannose to GDP-fucose, involving an epimerase and a reductase reaction [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fucose synthase family [H]

Homologues:

Organism=Homo sapiens, GI4507709, Length=320, Percent_Identity=29.0625, Blast_Score=148, Evalue=6e-36,
Organism=Homo sapiens, GI7657641, Length=355, Percent_Identity=21.6901408450704, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1788365, Length=318, Percent_Identity=78.3018867924528, Blast_Score=540, Evalue=1e-155,
Organism=Escherichia coli, GI1788353, Length=356, Percent_Identity=24.438202247191, Blast_Score=68, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17554428, Length=321, Percent_Identity=30.8411214953271, Blast_Score=159, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI71982038, Length=304, Percent_Identity=23.3552631578947, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI71982035, Length=304, Percent_Identity=23.3552631578947, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI19922778, Length=326, Percent_Identity=28.8343558282209, Blast_Score=149, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =1.1.1.271 [H]

Molecular weight: Translated: 39434; Mature: 39434

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQLKSILFSNHMVTRFQFLWSKEMTIKKRVFVAGYNGMVGSAIVRQLKNRDDIELVLRS
CHHHHHHHHCCHHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHH
RQELNLLNAQDVNHFFANERINEVYLAAAKVGGIVANNTYPADFIYENILIEANIIHAAH
HHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHCCEEECCCCCHHHHHHHEEEEEEEEEEEH
VNDVNKIMFLGSSCIYPKFAKQPISESELLQGTLESTNEPYAIAKIAGIKLCESYNRQHN
HCCHHHEEEECCCCCCCHHHCCCCCHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHCHHCC
RDYRSVMPTNLYGENDNFHPSNSHVIPALLRRFHEAKLEGLPQVVVWGSGTPMREFLHVD
CHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHC
DMAAASIYVMDLDKSIWQEQTQPMLSHINVGTGVDCSIRELAETISKVVGYRGEVVFDST
CHHHHEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECC
KPDGTPRKLLDVSRLEGLGWKYQIKLEAGLTRTYEWFLKNQQSFRS
CCCCCHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHCCHHHHCC
>Mature Secondary Structure
MRQLKSILFSNHMVTRFQFLWSKEMTIKKRVFVAGYNGMVGSAIVRQLKNRDDIELVLRS
CHHHHHHHHCCHHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHH
RQELNLLNAQDVNHFFANERINEVYLAAAKVGGIVANNTYPADFIYENILIEANIIHAAH
HHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHCCEEECCCCCHHHHHHHEEEEEEEEEEEH
VNDVNKIMFLGSSCIYPKFAKQPISESELLQGTLESTNEPYAIAKIAGIKLCESYNRQHN
HCCHHHEEEECCCCCCCHHHCCCCCHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHCHHCC
RDYRSVMPTNLYGENDNFHPSNSHVIPALLRRFHEAKLEGLPQVVVWGSGTPMREFLHVD
CHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHC
DMAAASIYVMDLDKSIWQEQTQPMLSHINVGTGVDCSIRELAETISKVVGYRGEVVFDST
CHHHHEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECC
KPDGTPRKLLDVSRLEGLGWKYQIKLEAGLTRTYEWFLKNQQSFRS
CCCCCHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7815923; 8759852; 9097040; 9278503; 9473059; 9862812 [H]