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The map label for this gene is otsA

Identifier: 238895454

GI number: 238895454

Start: 3340310

End: 3341734

Strand: Reverse

Name: otsA

Synonym: KP1_3523

Alternate gene names: 238895454

Gene position: 3341734-3340310 (Counterclockwise)

Preceding gene: 238895455

Following gene: 238895452

Centisome position: 63.67

GC content: 57.47

Gene sequence:

>1425_bases
ATGAGTCGTTTAGTCGTAGTCTCTAACCGTATCGCCTTACCGGACGATAAAAAATCGAGCGCTGGCGGTCTGGCCGTCGG
CATCCTGGGGGCACTGAGAGCGGCGGGGGGTCTGTGGTTTGGCTGGAGCGGCGAGATTGGCGATGACCAGCAGCCGCTCA
AGCAGGTCTCCCGCGGGAATATTTCCTGGGCGTCGTTCAACCTTAATGAACGGGACCATGATGAATACTACAACCAGTTC
TCTAACGCCGTGCTGTGGCCGGCTTTCCACTATCGCCTTGATCTGGTCAGCTTTCAGCGCGAAGCCTGGGAGGGATATCT
GCGGGTTAACGCCATGCTGGCGGACAAGCTTCTGCCGCTGATTGAGCCAGACGATACGCTGTGGATCCATGATTACCATC
TTCTGCCCTTCGCCAGCGAGCTGCGTAAGCGCGGAGTGAACAATCGCATCGGGTTTTTCTTACATATTCCGTTCCCGACG
CCGGAGATTTTTAACGCTCTGCCGCCGCACGCTGAGCTGCTGGAGCAACTGTGCGATTACGATCTGCTGGGCTTCCAGAC
CGAAAGCGACAGAACCGCTTTTCTCGACAGCATTGCTATGCAGACTCGCCTATCCGACCTCGGCGATAAACGCTATCAGG
CATGGGGTAAGGCGTTCAGTACCGAGGTTTATCCGATAGGGATCGATCCGGACGAAATTACGCGCAACGCTAAAGGTCCG
CTGCCGCCGAAGCTGGCCCAGCTGAAGAACGAGCTGAAAAATGTGAAGAATATCTTCTCCGTGGAGCGTCTTGACTATTC
GAAAGGGTTGCCGGAGCGATTCCTCGCCTACGAGACGCTGCTCGAGAAGTACCCTCAGCACCACGGCAAAATCCGTTATA
CCCAGATCGCGCCGACCTCCCGCGGCGATGTGCAGGCCTATCAGGATATCCGCCACCAGCTGGAAACCGCAGCCGGACGC
ATCAACGGACAGTTTGGTCAGCTTGGCTGGACACCGCTCTACTATCTGAATCAGCATTTTGATCGTAAGCTGCTGATGAA
GGTCTTCCGCTACTCTGACGTCGGGCTGGTGACGCCGCTACGCGATGGTATGAATCTGGTGGCGAAGGAGTACGTCGCGG
CGCAGGATCCGGATAACCCTGGCGTACTGGTCCTGTCGCAGTTTGCCGGGGCGGCGCAGGAGCTGACCAGCGCCCTGATC
GTCAATCCTTACGATCGCGACGAGGTTGCCGCCGCGCTGGATCGCGCGCTCAGCATGCCGCTGGCGGAACGTATCGCCCG
TCATTCAGCGATGCTGGACGTGATCAGAGAAAATGATATTCATAACTGGCAGGCGCGCTTTGTCGAGGATCTGCAGCATA
TTTCACCGCGCAGCGAGGAGAGCCGTCTGCGGGGGAAGATAGCCACCTTCCCTAAACTGGCCTAG

Upstream 100 bases:

>100_bases
GGGGAAACTCAGGCGCATTGGCGGTTGGCGGATGCCGCCGCTGTAAGGACGTGGTTGCAACATCTGGCGTATGACGCGCA
AACCGAAAGGAGGGATGACC

Downstream 100 bases:

>100_bases
TTCAGGAAAAGGGCTAACCGGCGTTAGCCCTTTTCAAGGGAAACCAACAGGACATCAACGCCGCAGCTGCCGACGATATT
TTTCGCCGAGCAGGTGGCGC

Product: trehalose-6-phosphate synthase

Products: NA

Alternate protein names: Osmoregulatory trehalose synthesis protein A; Trehalose-6-phosphate synthase; UDP-glucose-glucosephosphate glucosyltransferase

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MSRLVVVSNRIALPDDKKSSAGGLAVGILGALRAAGGLWFGWSGEIGDDQQPLKQVSRGNISWASFNLNERDHDEYYNQF
SNAVLWPAFHYRLDLVSFQREAWEGYLRVNAMLADKLLPLIEPDDTLWIHDYHLLPFASELRKRGVNNRIGFFLHIPFPT
PEIFNALPPHAELLEQLCDYDLLGFQTESDRTAFLDSIAMQTRLSDLGDKRYQAWGKAFSTEVYPIGIDPDEITRNAKGP
LPPKLAQLKNELKNVKNIFSVERLDYSKGLPERFLAYETLLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLETAAGR
INGQFGQLGWTPLYYLNQHFDRKLLMKVFRYSDVGLVTPLRDGMNLVAKEYVAAQDPDNPGVLVLSQFAGAAQELTSALI
VNPYDRDEVAAALDRALSMPLAERIARHSAMLDVIRENDIHNWQARFVEDLQHISPRSEESRLRGKIATFPKLA

Sequences:

>Translated_474_residues
MSRLVVVSNRIALPDDKKSSAGGLAVGILGALRAAGGLWFGWSGEIGDDQQPLKQVSRGNISWASFNLNERDHDEYYNQF
SNAVLWPAFHYRLDLVSFQREAWEGYLRVNAMLADKLLPLIEPDDTLWIHDYHLLPFASELRKRGVNNRIGFFLHIPFPT
PEIFNALPPHAELLEQLCDYDLLGFQTESDRTAFLDSIAMQTRLSDLGDKRYQAWGKAFSTEVYPIGIDPDEITRNAKGP
LPPKLAQLKNELKNVKNIFSVERLDYSKGLPERFLAYETLLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLETAAGR
INGQFGQLGWTPLYYLNQHFDRKLLMKVFRYSDVGLVTPLRDGMNLVAKEYVAAQDPDNPGVLVLSQFAGAAQELTSALI
VNPYDRDEVAAALDRALSMPLAERIARHSAMLDVIRENDIHNWQARFVEDLQHISPRSEESRLRGKIATFPKLA
>Mature_473_residues
SRLVVVSNRIALPDDKKSSAGGLAVGILGALRAAGGLWFGWSGEIGDDQQPLKQVSRGNISWASFNLNERDHDEYYNQFS
NAVLWPAFHYRLDLVSFQREAWEGYLRVNAMLADKLLPLIEPDDTLWIHDYHLLPFASELRKRGVNNRIGFFLHIPFPTP
EIFNALPPHAELLEQLCDYDLLGFQTESDRTAFLDSIAMQTRLSDLGDKRYQAWGKAFSTEVYPIGIDPDEITRNAKGPL
PPKLAQLKNELKNVKNIFSVERLDYSKGLPERFLAYETLLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLETAAGRI
NGQFGQLGWTPLYYLNQHFDRKLLMKVFRYSDVGLVTPLRDGMNLVAKEYVAAQDPDNPGVLVLSQFAGAAQELTSALIV
NPYDRDEVAAALDRALSMPLAERIARHSAMLDVIRENDIHNWQARFVEDLQHISPRSEESRLRGKIATFPKLA

Specific function: Catalyzes the transfer of glucose from UDP-glucose to glucose-6-phosphate to form alpha,alpha-1,1 trehalose-6-phosphate. Acts with retention of the anomeric configuration of the UDP-sugar donor

COG id: COG0380

COG function: function code G; Trehalose-6-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 20 family

Homologues:

Organism=Escherichia coli, GI1788206, Length=474, Percent_Identity=82.0675105485232, Blast_Score=793, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71987012, Length=440, Percent_Identity=30.6818181818182, Blast_Score=176, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI115533560, Length=356, Percent_Identity=32.3033707865169, Blast_Score=163, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI115533558, Length=356, Percent_Identity=32.3033707865169, Blast_Score=163, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6319602, Length=470, Percent_Identity=35.7446808510638, Blast_Score=291, Evalue=1e-79,
Organism=Saccharomyces cerevisiae, GI6323537, Length=387, Percent_Identity=29.7157622739018, Blast_Score=176, Evalue=9e-45,
Organism=Saccharomyces cerevisiae, GI6323917, Length=394, Percent_Identity=28.9340101522843, Blast_Score=175, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6320279, Length=405, Percent_Identity=27.4074074074074, Blast_Score=170, Evalue=4e-43,
Organism=Drosophila melanogaster, GI19920676, Length=486, Percent_Identity=34.9794238683128, Blast_Score=280, Evalue=1e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): OTSA_KLEP7 (A6TB47)

Other databases:

- EMBL:   CP000647
- RefSeq:   YP_001336048.1
- ProteinModelPortal:   A6TB47
- SMR:   A6TB47
- STRING:   A6TB47
- GeneID:   5338205
- GenomeReviews:   CP000647_GR
- KEGG:   kpn:KPN_02392
- eggNOG:   COG0380
- HOGENOM:   HBG559076
- OMA:   CHYRLDL
- ProtClustDB:   PRK10117
- InterPro:   IPR001830
- InterPro:   IPR012766
- TIGRFAMs:   TIGR02400

Pfam domain/function: PF00982 Glyco_transf_20

EC number: =2.4.1.15

Molecular weight: Translated: 53814; Mature: 53683

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: NA

Important sites: BINDING 10-10 BINDING 77-77 BINDING 131-131 BINDING 263-263 BINDING 268-268 BINDING 301-301 BINDING 340-340

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLVVVSNRIALPDDKKSSAGGLAVGILGALRAAGGLWFGWSGEIGDDQQPLKQVSRGN
CCCEEEEECCEECCCCCCCCCCCHHHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHHCCC
ISWASFNLNERDHDEYYNQFSNAVLWPAFHYRLDLVSFQREAWEGYLRVNAMLADKLLPL
CEEEEECCCCCCHHHHHHHHHCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
IEPDDTLWIHDYHLLPFASELRKRGVNNRIGFFLHIPFPTPEIFNALPPHAELLEQLCDY
CCCCCEEEEEEEECCCHHHHHHHCCCCCCCEEEEECCCCCHHHHHCCCCHHHHHHHHCCC
DLLGFQTESDRTAFLDSIAMQTRLSDLGDKRYQAWGKAFSTEVYPIGIDPDEITRNAKGP
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHCCCCCC
LPPKLAQLKNELKNVKNIFSVERLDYSKGLPERFLAYETLLEKYPQHHGKIRYTQIAPTS
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCC
RGDVQAYQDIRHQLETAAGRINGQFGQLGWTPLYYLNQHFDRKLLMKVFRYSDVGLVTPL
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHCCCCCEECCH
RDGMNLVAKEYVAAQDPDNPGVLVLSQFAGAAQELTSALIVNPYDRDEVAAALDRALSMP
HHHHHHHHHHHHHCCCCCCCCEEEEHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCH
LAERIARHSAMLDVIRENDIHNWQARFVEDLQHISPRSEESRLRGKIATFPKLA
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHCHHCCCCCCC
>Mature Secondary Structure 
SRLVVVSNRIALPDDKKSSAGGLAVGILGALRAAGGLWFGWSGEIGDDQQPLKQVSRGN
CCEEEEECCEECCCCCCCCCCCHHHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHHCCC
ISWASFNLNERDHDEYYNQFSNAVLWPAFHYRLDLVSFQREAWEGYLRVNAMLADKLLPL
CEEEEECCCCCCHHHHHHHHHCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
IEPDDTLWIHDYHLLPFASELRKRGVNNRIGFFLHIPFPTPEIFNALPPHAELLEQLCDY
CCCCCEEEEEEEECCCHHHHHHHCCCCCCCEEEEECCCCCHHHHHCCCCHHHHHHHHCCC
DLLGFQTESDRTAFLDSIAMQTRLSDLGDKRYQAWGKAFSTEVYPIGIDPDEITRNAKGP
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHCCCCCC
LPPKLAQLKNELKNVKNIFSVERLDYSKGLPERFLAYETLLEKYPQHHGKIRYTQIAPTS
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCC
RGDVQAYQDIRHQLETAAGRINGQFGQLGWTPLYYLNQHFDRKLLMKVFRYSDVGLVTPL
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHCCCCCEECCH
RDGMNLVAKEYVAAQDPDNPGVLVLSQFAGAAQELTSALIVNPYDRDEVAAALDRALSMP
HHHHHHHHHHHHHCCCCCCCCEEEEHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCH
LAERIARHSAMLDVIRENDIHNWQARFVEDLQHISPRSEESRLRGKIATFPKLA
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA