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The map label for this gene is ccmE

Identifier: 238895113

GI number: 238895113

Start: 2991540

End: 2991998

Strand: Reverse

Name: ccmE

Synonym: KP1_3152

Alternate gene names: 238895113

Gene position: 2991998-2991540 (Counterclockwise)

Preceding gene: 238895114

Following gene: 238895112

Centisome position: 57.01

GC content: 62.31

Gene sequence:

>459_bases
ATGCAAGCGCGTCGTAAAACCCGGCTGTATATCGTGCTGGCGGTGCTCGCCGGACTTGGGCTCACCGTCAGCCTGACGCT
GTACGCCCTGAGCAGCAATATCGATCTGTTCTACACCCCGGGGGAAATTATCTACGGCAAAACGGAAACCCGGGCGCTGC
CCCATACCGGTCAGCGGCTGCGGGTGGGAGGCTACGTTCAGCCGGGCTCCCTGCAGCGCGACCCGCAAACCCTCGACGTG
CGCTTTAAGCTGTATGACGCCCGCGGGGTGGTGGACGTCAGCTATAAGGGCATTCTGCCGGACCTGTTTCGTGAAGGGCA
GGGCGTGGTGGCCCAGGGCGTACTGGACGGCGAGCGGCATATTACCGCCCAGCAGGTGCTGGCCAAACATGATGAAAATT
ACACGCCGCCGGAGGTGAAAAACGCCATGACGCCGGAGAAAACGGGGGCGCAGCCATGA

Upstream 100 bases:

>100_bases
ACCCTGTGGGCGCGGCGGGCGCTGTTCCATGAGGTACGACGCCAGCAGGCGCGCGAGCGGCGGATCGCTGCCGCCCGCGA
ACATGACAAGGAGGCCGCGG

Downstream 100 bases:

>100_bases
TGCCGGAACTGGGCAACTTTTTATTGTGTCTGGCGGCGGGGCTGGCGCTGCTCCTGAGCGTCTATCCCCTGTGGGGCGCG
GCGCGGCAGGATCGGCGGCT

Product: cytochrome c-type biogenesis heme-carrier protein

Products: NA

Alternate protein names: Cytochrome c maturation protein E; Heme chaperone CcmE

Number of amino acids: Translated: 152; Mature: 152

Protein sequence:

>152_residues
MQARRKTRLYIVLAVLAGLGLTVSLTLYALSSNIDLFYTPGEIIYGKTETRALPHTGQRLRVGGYVQPGSLQRDPQTLDV
RFKLYDARGVVDVSYKGILPDLFREGQGVVAQGVLDGERHITAQQVLAKHDENYTPPEVKNAMTPEKTGAQP

Sequences:

>Translated_152_residues
MQARRKTRLYIVLAVLAGLGLTVSLTLYALSSNIDLFYTPGEIIYGKTETRALPHTGQRLRVGGYVQPGSLQRDPQTLDV
RFKLYDARGVVDVSYKGILPDLFREGQGVVAQGVLDGERHITAQQVLAKHDENYTPPEVKNAMTPEKTGAQP
>Mature_152_residues
MQARRKTRLYIVLAVLAGLGLTVSLTLYALSSNIDLFYTPGEIIYGKTETRALPHTGQRLRVGGYVQPGSLQRDPQTLDV
RFKLYDARGVVDVSYKGILPDLFREGQGVVAQGVLDGERHITAQQVLAKHDENYTPPEVKNAMTPEKTGAQP

Specific function: Heme chaperone required for the biogenesis of c-type cytochromes. Transiently binds heme delivered by CcmC and transfers the heme to apo-cytochromes in a process facilitated by CcmF and CcmH

COG id: COG2332

COG function: function code O; Cytochrome c-type biogenesis protein CcmE

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein; Periplasmic side (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CcmE/CycJ family

Homologues:

Organism=Escherichia coli, GI1788525, Length=143, Percent_Identity=71.3286713286713, Blast_Score=214, Evalue=2e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CCME_KLEP3 (B5XWC8)

Other databases:

- EMBL:   CP000964
- RefSeq:   YP_002238092.1
- ProteinModelPortal:   B5XWC8
- SMR:   B5XWC8
- GeneID:   6935635
- GenomeReviews:   CP000964_GR
- KEGG:   kpe:KPK_2256
- HOGENOM:   HBG296631
- OMA:   HVEFAVH
- ProtClustDB:   CLSK2400778
- HAMAP:   MF_01959
- InterPro:   IPR004329
- InterPro:   IPR012340
- Gene3D:   G3DSA:2.40.50.140

Pfam domain/function: PF03100 CcmE; SSF82093 Pyrv/PenolPyrv_Kinase_cat

EC number: NA

Molecular weight: Translated: 16766; Mature: 16766

Theoretical pI: Translated: 9.54; Mature: 9.54

Prosite motif: NA

Important sites: BINDING 130-130

Signals:

None

Transmembrane regions:

HASH(0x1bd08ccc)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQARRKTRLYIVLAVLAGLGLTVSLTLYALSSNIDLFYTPGEIIYGKTETRALPHTGQRL
CCCCHHHHHHHHHHHHHCCCCEEEEEEEEEECCCEEEECCCEEEECCCCCCCCCCCCCEE
RVGGYVQPGSLQRDPQTLDVRFKLYDARGVVDVSYKGILPDLFREGQGVVAQGVLDGERH
EECCEECCCCCCCCCCEEEEEEEEEECCCEEEEECCCCCHHHHHCCCCEEEECEECCCHH
ITAQQVLAKHDENYTPPEVKNAMTPEKTGAQP
HHHHHHHHHCCCCCCCCHHHHCCCCCCCCCCH
>Mature Secondary Structure
MQARRKTRLYIVLAVLAGLGLTVSLTLYALSSNIDLFYTPGEIIYGKTETRALPHTGQRL
CCCCHHHHHHHHHHHHHCCCCEEEEEEEEEECCCEEEECCCEEEECCCCCCCCCCCCCEE
RVGGYVQPGSLQRDPQTLDVRFKLYDARGVVDVSYKGILPDLFREGQGVVAQGVLDGERH
EECCEECCCCCCCCCCEEEEEEEEEECCCEEEEECCCCCHHHHHCCCCEEEECEECCCHH
ITAQQVLAKHDENYTPPEVKNAMTPEKTGAQP
HHHHHHHHHCCCCCCCCHHHHCCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA